UniProtKB - O73947 (PCNA_PYRFU)
DNA polymerase sliding clamp
pcn
Functioni
Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Unlike its eukaryotic paralog, loads on circular DNA without the replication factor C (RFC) clamp loader, although RFC greatly increases loading efficiency. Stimulates the ATPase activity of replication factor C (RFC) in the presence of ssDNA. Stimulates the helicase activity of Hel308 and may alter its substrate specificity.
3 PublicationsGO - Molecular functioni
- DNA binding Source: UniProtKB-UniRule
- DNA polymerase processivity factor activity Source: UniProtKB-UniRule
GO - Biological processi
- DNA replication Source: UniProtKB-KW
- regulation of DNA replication Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase sliding clampUniRule annotationAlternative name(s): Proliferating cell nuclear antigen homologUniRule annotation Short name: PCNAUniRule annotation |
Gene namesi | Name:pcnUniRule annotation Ordered Locus Names:PF0983 |
Organismi | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
Taxonomic identifieri | 186497 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 73 | M → L: No observable effect. 1 Publication | 1 | |
Mutagenesisi | 143 – 147 | DAVKD → AAVKA: No homotrimer formation. Stimulates ATPase activity of RFC, no stimulation of DNA synthesis by Pol I in presence and absence of RFC. Crystallizes as tail-to-tail homodimers. 1 Publication | 5 | |
Mutagenesisi | 143 | D → A: Small amount of homotrimer in solution. Stimulates ATPase activity of RFC and DNA synthesis by Pol I in presence and absence of RFC. Crystallizes as tail-to-tail homodimers. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149206 | 1 – 249 | DNA polymerase sliding clampAdd BLAST | 249 |
Proteomic databases
PRIDEi | O73947 |
Interactioni
Subunit structurei
Homotrimer which circularizes head-to-tail (head is a N-terminus, tail is at C-terminus) to form a toroid. RFC opens the toroid so it can load on DNA.
Interacts with both Pol I (pol) and Pol II (polB-polC), with Hel308 (hjm) and with Hjc. Interaction with the C-terminal PIP-box of RfcL may stabilize the toroidal structure.
5 PublicationsBinary interactionsi
O73947
With | #Exp. | IntAct |
---|---|---|
polB [P81412] | 2 | EBI-15762053,EBI-15906191 |
Protein-protein interaction databases
DIPi | DIP-48777N |
IntActi | O73947, 2 interactors |
STRINGi | 186497.PF0983 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O73947 |
SMRi | O73947 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O73947 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | arCOG00488, Archaea |
HOGENOMi | CLU_043978_1_0_2 |
OMAi | EMKLINM |
OrthoDBi | 70433at2157 |
PhylomeDBi | O73947 |
Family and domain databases
HAMAPi | MF_00317, DNApol_clamp_arch, 1 hit |
IDEALi | IID90015 |
InterProi | View protein in InterPro IPR000730, Pr_cel_nuc_antig IPR022649, Pr_cel_nuc_antig_C IPR022659, Pr_cel_nuc_antig_CS IPR022648, Pr_cel_nuc_antig_N |
PANTHERi | PTHR11352:SF0, PTHR11352:SF0, 1 hit |
Pfami | View protein in Pfam PF02747, PCNA_C, 1 hit PF00705, PCNA_N, 1 hit |
PRINTSi | PR00339, PCNACYCLIN |
TIGRFAMsi | TIGR00590, pcna, 1 hit |
PROSITEi | View protein in PROSITE PS01251, PCNA_1, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MPFEIVFEGA KEFAQLIDTA SKLIDEAAFK VTEDGISMRA MDPSRVVLID
60 70 80 90 100
LNLPSSIFSK YEVVEPETIG VNMDHLKKIL KRGKAKDTLI LKKGEENFLE
110 120 130 140 150
ITIQGTATRT FRVPLIDVEE MEVDLPELPF TAKVVVLGEV LKDAVKDASL
160 170 180 190 200
VSDSIKFIAR ENEFIMKAEG ETQEVEIKLT LEDEGLLDIE VQEETKSAYG
210 220 230 240
VSYLSDMVKG LGKADEVTIK FGNEMPMQME YYIRDEGRLT FLLAPRVEE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB017486 Genomic DNA Translation: BAA33020.2 AE009950 Genomic DNA Translation: AAL81107.1 |
RefSeqi | WP_011012120.1, NC_018092.1 |
Genome annotation databases
EnsemblBacteriai | AAL81107; AAL81107; PF0983 |
GeneIDi | 41712796 |
KEGGi | pfu:PF0983 |
PATRICi | fig|186497.12.peg.1042 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB017486 Genomic DNA Translation: BAA33020.2 AE009950 Genomic DNA Translation: AAL81107.1 |
RefSeqi | WP_011012120.1, NC_018092.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GE8 | X-ray | 2.10 | A | 1-249 | [»] | |
1ISQ | X-ray | 2.30 | A | 1-249 | [»] | |
1IZ4 | X-ray | 2.00 | A | 1-249 | [»] | |
1IZ5 | X-ray | 1.80 | A/B | 1-249 | [»] | |
3A2F | X-ray | 2.67 | B | 2-249 | [»] | |
5AUJ | X-ray | 2.50 | A | 1-249 | [»] | |
AlphaFoldDBi | O73947 | |||||
SMRi | O73947 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-48777N |
IntActi | O73947, 2 interactors |
STRINGi | 186497.PF0983 |
Proteomic databases
PRIDEi | O73947 |
Genome annotation databases
EnsemblBacteriai | AAL81107; AAL81107; PF0983 |
GeneIDi | 41712796 |
KEGGi | pfu:PF0983 |
PATRICi | fig|186497.12.peg.1042 |
Phylogenomic databases
eggNOGi | arCOG00488, Archaea |
HOGENOMi | CLU_043978_1_0_2 |
OMAi | EMKLINM |
OrthoDBi | 70433at2157 |
PhylomeDBi | O73947 |
Miscellaneous databases
EvolutionaryTracei | O73947 |
Family and domain databases
HAMAPi | MF_00317, DNApol_clamp_arch, 1 hit |
IDEALi | IID90015 |
InterProi | View protein in InterPro IPR000730, Pr_cel_nuc_antig IPR022649, Pr_cel_nuc_antig_C IPR022659, Pr_cel_nuc_antig_CS IPR022648, Pr_cel_nuc_antig_N |
PANTHERi | PTHR11352:SF0, PTHR11352:SF0, 1 hit |
Pfami | View protein in Pfam PF02747, PCNA_C, 1 hit PF00705, PCNA_N, 1 hit |
PRINTSi | PR00339, PCNACYCLIN |
TIGRFAMsi | TIGR00590, pcna, 1 hit |
PROSITEi | View protein in PROSITE PS01251, PCNA_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PCNA_PYRFU | |
Accessioni | O73947Primary (citable) accession number: O73947 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | February 21, 2001 | |
Last modified: | May 25, 2022 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families