UniProtKB - O73875 (EPB4A_DANRE)
Ephrin type-B receptor 4a
ephb4a
Functioni
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 is involved in the regulation of cell adhesion and cell migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability (PubMed:29444212).
EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells (By similarity) (PubMed:29444212).
Involved in somitogenesis (PubMed:9765210).
By similarity2 PublicationsCatalytic activityi
- EC:2.7.10.1PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 653 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 746 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 627 – 635 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ephrin receptor activity Source: InterPro
- protein serine/threonine/tyrosine kinase activity Source: RHEA
GO - Biological processi
- angioblast cell migration involved in selective angioblast sprouting Source: ZFIN
- blood vessel development Source: ZFIN
- convergent extension Source: ZFIN
- ephrin receptor signaling pathway Source: ZFIN
- lymph vessel development Source: ZFIN
- venous blood vessel morphogenesis Source: ZFIN
Keywordsi
Molecular function | Kinase, Receptor, Transferase, Tyrosine-protein kinase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DRE-2682334, EPH-Ephrin signaling R-DRE-3928662, EPHB-mediated forward signaling R-DRE-3928664, Ephrin signaling R-DRE-3928665, EPH-ephrin mediated repulsion of cells |
Names & Taxonomyi
Protein namesi | Recommended name: Ephrin type-B receptor 4aBy similarity (EC:2.7.10.1PROSITE-ProRule annotation)Alternative name(s): Eph receptor B4aImported Eph-like receptor tyrosine kinase rtk5Imported |
Gene namesi | |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-990415-62, ephb4a |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: InterPro
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 25 – 548 | ExtracellularSequence analysisAdd BLAST | 524 | |
Transmembranei | 549 – 569 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 570 – 987 | CytoplasmicSequence analysisAdd BLAST | 418 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_5004159945 | 25 – 987 | Ephrin type-B receptor 4aSequence analysisAdd BLAST | 963 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 70 ↔ 187 | By similarity | ||
Disulfide bondi | 104 ↔ 114 | By similarity |
Keywords - PTMi
Disulfide bondFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 205 | Eph LBDPROSITE-ProRule annotationAdd BLAST | 180 | |
Domaini | 328 – 438 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 111 | |
Domaini | 442 – 536 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 621 – 884 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 264 | |
Domaini | 914 – 978 | SAMPROSITE-ProRule annotationAdd BLAST | 65 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 319 – 340 | DisorderedSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00940000166305 |
OrthoDBi | 933071at2759 |
PhylomeDBi | O73875 |
Family and domain databases
CDDi | cd10474, EphR_LBD_B4, 1 hit cd00063, FN3, 2 hits cd09554, SAM_EPH-B4, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR037636, EPH-B4_SAM IPR027936, Eph_TM IPR034290, EphB4_rcpt_lig-bd IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF00041, fn3, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MELFSRNVAA FWIILLEFLL GSVAEEEVLM NTKTETSDLK WTTHSRSKPE
60 70 80 90 100
WEEVSGLDEE NNSVRTYQIC QADGSSSHWL RSKLIERRGA SQVYVELFFT
110 120 130 140 150
MVECSSRNTH HRSCKETFNL YYYQSDTDDA TATHPAWMEN PYTKVDTVAA
160 170 180 190 200
DFLLRKGGEK KVNVKTLRLG PLSKRGFYLA FQAQGACMAL LSVRVFFKKC
210 220 230 240 250
PALTRSLSVF PETVPRSLVQ EAVGQCVANA AQPGPSPRPP KMFCGEDGQW
260 270 280 290 300
VDQPTTTCTC LPGFEASHGE LECRACPVGL FKMGSGTGPC SVCPENSQTG
310 320 330 340 350
ETGSAACVCR SGFYRALSDS ADTPCTRPPS SPRSPVPQVN DTSLTLEWSE
360 370 380 390 400
PLDSGGRSDL SYSVECRMCS TPGSPCTLCS DGVNYRPSQT GIQGRRVSIW
410 420 430 440 450
GLRPHTTYSF TVMALNGVSA QSQQGPAGET INITTSPNVP VLVSGLRKST
460 470 480 490 500
ATESSLTLYW NTPTQSHYRI LQYQIRYCEK ERGSEENSCH YMESNNNEVV
510 520 530 540 550
LSDLRRATQY EVQVRARTFA GYGSFGKAIL FRTLPDEDDS SSPLLVTGIL
560 570 580 590 600
IAMGMLLLII VIGAAIYCIR KQNNYKDPEL SDKNGQYLMG QGVKVYIDPF
610 620 630 640 650
TYEDPNEAVR EFAKEIDVSC VKIEEVIGAG EFGEVCRGRL RIPGKKENYV
660 670 680 690 700
AIKTLKGGYT DKQRRDFLAE ASIMGQFQHP NIIHLEGIIT ASCPVMILTE
710 720 730 740 750
FMENGALDSF LRLNDGQFTP IQLVGMLRGI ASGMKYLSEM SYVHRDLAAR
760 770 780 790 800
NILVNSNLVC KVSDFGLSRF LQENSSDPTY TSSLGGKIPI RWTAPEAIAF
810 820 830 840 850
RKFTCASDVW SYGIVMWEVM SFGERPYWDM SNQDVINAIE QDYRLPPPPD
860 870 880 890 900
CPTYLHQLML DCWQKERTAR PRFANIVSAL DKLIRNPASL KITAQEGAGP
910 920 930 940 950
SHPLLDQRSP LTPSSCGTVG DWLRAIKMER YEETFLQAGY TSMQLVTHIN
960 970 980
TEDLLRLGIT LAGHQKKILS SIEALGIQNK APGNVLY
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4IJK3 | A0A0R4IJK3_DANRE | Receptor protein-tyrosine kinase | ephb4a | 960 | Annotation score: | ||
A4FUN2 | A4FUN2_DANRE | Ephrin type-B receptor 4a | ephb4a LOC100000756 | 293 | Annotation score: | ||
A0A0R4INV2 | A0A0R4INV2_DANRE | Ephrin type-B receptor 4a | ephb4a | 268 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 13 | I → V in CAA06299 (PubMed:27747378). | 1 | |
Sequence conflicti | 79 – 80 | WL → CV in CAA06299 (PubMed:27747378). | 2 | |
Sequence conflicti | 852 | P → S in CAA06299 (PubMed:27747378). | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005026 mRNA Translation: CAA06299.2 CABZ01093042 Genomic DNA No translation available. FO904976 Genomic DNA No translation available. CABZ01093043 Genomic DNA No translation available. CABZ01112153 Genomic DNA No translation available. CABZ01112154 Genomic DNA No translation available. CABZ01112155 Genomic DNA No translation available. CABZ01112156 Genomic DNA No translation available. CABZ01112157 Genomic DNA No translation available. CU682352 Genomic DNA No translation available. CU928075 Genomic DNA No translation available. |
RefSeqi | NP_571489.1, NM_131414.1 |
Genome annotation databases
Ensembli | ENSDART00000187955; ENSDARP00000152897; ENSDARG00000100725 |
GeneIDi | 30688 |
KEGGi | dre:30688 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005026 mRNA Translation: CAA06299.2 CABZ01093042 Genomic DNA No translation available. FO904976 Genomic DNA No translation available. CABZ01093043 Genomic DNA No translation available. CABZ01112153 Genomic DNA No translation available. CABZ01112154 Genomic DNA No translation available. CABZ01112155 Genomic DNA No translation available. CABZ01112156 Genomic DNA No translation available. CABZ01112157 Genomic DNA No translation available. CU682352 Genomic DNA No translation available. CU928075 Genomic DNA No translation available. |
RefSeqi | NP_571489.1, NM_131414.1 |
3D structure databases
AlphaFoldDBi | O73875 |
SMRi | O73875 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000111946 |
Genome annotation databases
Ensembli | ENSDART00000187955; ENSDARP00000152897; ENSDARG00000100725 |
GeneIDi | 30688 |
KEGGi | dre:30688 |
Organism-specific databases
CTDi | 30688 |
ZFINi | ZDB-GENE-990415-62, ephb4a |
Phylogenomic databases
GeneTreei | ENSGT00940000166305 |
OrthoDBi | 933071at2759 |
PhylomeDBi | O73875 |
Enzyme and pathway databases
Reactomei | R-DRE-2682334, EPH-Ephrin signaling R-DRE-3928662, EPHB-mediated forward signaling R-DRE-3928664, Ephrin signaling R-DRE-3928665, EPH-ephrin mediated repulsion of cells |
Miscellaneous databases
PROi | PR:O73875 |
Gene expression databases
ExpressionAtlasi | O73875, baseline and differential |
Family and domain databases
CDDi | cd10474, EphR_LBD_B4, 1 hit cd00063, FN3, 2 hits cd09554, SAM_EPH-B4, 1 hit |
Gene3Di | 1.10.150.50, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR037636, EPH-B4_SAM IPR027936, Eph_TM IPR034290, EphB4_rcpt_lig-bd IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF00041, fn3, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EPB4A_DANRE | |
Accessioni | O73875Primary (citable) accession number: O73875 Secondary accession number(s): A0A2R8QG60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 5, 2019 |
Last sequence update: | June 5, 2019 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families