Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine-protein kinase receptor Tie-2

Gene

tie2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Tyrosine-protein kinase that acts as cell-surface receptor for angiopoietins and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Can activate or inhibit angiogenesis, depending on the context. Angiopoietin signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Activity regulationi

Angiopoietin binding leads to receptor dimerization and activation by autophosphorylation at Tyr-984 on the kinase activation loop.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei847ATPPROSITE-ProRule annotation1
Active sitei956Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi822 – 830ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • angiogenesis Source: ZFIN
  • heart development Source: ZFIN
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: InterPro

Keywordsi

Molecular functionKinase, Receptor, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1 928

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase receptor Tie-2 (EC:2.7.10.1)
Alternative name(s):
Tyrosine kinase with Ig and EGF homology domains-2
Gene namesi
Name:tie2
Synonyms:tie-2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-990415-56 tek

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 745ExtracellularSequence analysisAdd BLAST724
Transmembranei746 – 766HelicalSequence analysisAdd BLAST21
Topological domaini767 – 1116CytoplasmicSequence analysisAdd BLAST350

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000002447622 – 1116Tyrosine-protein kinase receptor Tie-2Add BLAST1095

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi46 ↔ 106By similarity
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi143N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi215 ↔ 224By similarity
Glycosylationi223N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi228 ↔ 237By similarity
Disulfide bondi231 ↔ 244By similarity
Disulfide bondi246 ↔ 255By similarity
Disulfide bondi259 ↔ 268By similarity
Disulfide bondi272 ↔ 277By similarity
Disulfide bondi283 ↔ 290By similarity
Disulfide bondi292 ↔ 301By similarity
Disulfide bondi305 ↔ 314By similarity
Disulfide bondi318 ↔ 325By similarity
Disulfide bondi320 ↔ 331By similarity
Disulfide bondi333 ↔ 341By similarity
Glycosylationi367N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi368 ↔ 422By similarity
Glycosylationi387N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi425N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi590N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi637N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi642N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei852Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei984Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1094Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1100Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner, where Tyr-984 in the kinase activation loop is phosphorylated first, followed by autophosphorylation at additional tyrosine residues. Phosphorylation is important for interaction with scaffold proteins and effectors.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO73791
PRIDEiO73791

Expressioni

Tissue specificityi

Expressed in most populations of endothelial cells in 24 hours embryos. Not present in intersegmental vessels.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000055680

Structurei

3D structure databases

ProteinModelPortaliO73791
SMRiO73791
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 126Ig-like C2-type 1Add BLAST81
Domaini214 – 256EGF-like 1PROSITE-ProRule annotationAdd BLAST43
Domaini258 – 302EGF-like 2PROSITE-ProRule annotationAdd BLAST45
Domaini304 – 342EGF-like 3PROSITE-ProRule annotationAdd BLAST39
Domaini348 – 438Ig-like C2-type 2Add BLAST91
Domaini444 – 538Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST95
Domaini540 – 633Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST94
Domaini634 – 729Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST96
Domaini816 – 1095Protein kinasePROSITE-ProRule annotationAdd BLAST280

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0200 Eukaryota
COG0515 LUCA
HOGENOMiHOG000049232
HOVERGENiHBG007316
InParanoidiO73791
KOiK05121
PhylomeDBiO73791

Family and domain databases

CDDicd00063 FN3, 2 hits
Gene3Di2.60.40.10, 6 hits
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR018941 Tyr_kin_Tie2_Ig-like_dom-1_N
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF00041 fn3, 2 hits
PF07679 I-set, 1 hit
PF10430 Ig_Tie2_1, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00060 FN3, 3 hits
SM00409 IG, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
SSF49265 SSF49265, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 1 hit
PS50853 FN3, 3 hits
PS50835 IG_LIKE, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O73791-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCLLDSCTAL LLLGCWMSGS AVRISDVTLV NPDPVVSPLT APSLLCVSSD
60 70 80 90 100
WSSGGSVLAL GQEFPRPQGS VLALGQEFPH TEPRPHPAAA TVTWSSRSHA
110 120 130 140 150
FGAFYCQIRN STGRKIYTYK MLQEAAFLPE SLTITVNQGE NINISYSRRL
160 170 180 190 200
YSPEDTVIHK NGHFEHSSPK EDISDIIHYP VTNAKAESHA GIYAIRYISA
210 220 230 240 250
APSSAAITRL IVRSCRAGFW GPNCTESCPR CANGGVCDDT TGECVCPPGF
260 270 280 290 300
RGHTCDIVCG EGRFGAGCKE RCVDGVCRAL VFCLRDPYGC SCASGWRGLS
310 320 330 340 350
CNDACPDGYY GAGCTQKCVC AKGRCDRFRG CVCAGRHGSR CEEADSSPVI
360 370 380 390 400
SHLRDVEINT GVELSVNCSA SGRPAPLHGD ITLITANRTT IAAVDTHTLN
410 420 430 440 450
DQSTSVFRVQ QVRVSSAGRW RCQVNNTHMQ VEDEFTVEVK VPPRPQNPPV
460 470 480 490 500
LQGSGPRHLL LLLNTEPYSG DGPIATTTLL YRPASAHTWS SVTAHGPLVR
510 520 530 540 550
LDNLYPMTQY LTQVQLSRPG PGGAGQAGPA ATFSTQVLEL PVGVKLSAVS
560 570 580 590 600
QTALLLSWDI APAEQHCTYE VSCLQAGAPG TLRTFQLPSN SSAMHLSDLK
610 620 630 640 650
PRHKYQCTVR SSCGVGQNHP SASAWTLSDQ LPPPPANISI WNISDTSAVL
660 670 680 690 700
TWAVAEGESV SRAVIRFQQV EQAQYRQQVE LPVQTQQLHM RFQLLGLRPN
710 720 730 740 750
TGYQLQLWTV NNMGESAESP PVSLMTLPQQ ESSALFAAHG HLLLYAILGS
760 770 780 790 800
AGMTCCTVLL AFCIVLQLKR NTLQRRIHSI LREEPAVHFS SAPPPHRRSA
810 820 830 840 850
VVSRSLVFPA LQWSDIQFQD VLGEGNFGQV LKARIRKDGL RMDAAVKRMK
860 870 880 890 900
DYASQDDHRD FAGELEVLCR LGPHKNIIHL LGACEHRGYL YLAIEFAPHG
910 920 930 940 950
NLLDFLRKSR VLETDPAFAI AHRTASTLSS QQLLAFSADV ARGMSYLSQK
960 970 980 990 1000
QFIHRDLAAR NVLVGENFVA KIADFGLSRG QEVYVKKTMG RLPVRWMAIE
1010 1020 1030 1040 1050
SLNYSVYTTN SDVWSYGVLL WEVVSLGGTP YCGMTCAELY EKLPLGFRLE
1060 1070 1080 1090 1100
KPLNCDDEVY ELMQQCWREK PFERPSFSQI LLSLGRMLEE RKTYVNTTLY
1110
EKFTYAGIDC SAEEAG
Length:1,116
Mass (Da):122,361
Last modified:August 1, 1998 - v1
Checksum:iAA414E8C745A8937
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B3DJR6B3DJR6_DANRE
Endothelium-specific receptor tyros...
tek tie2
1,116Annotation score:
F8W2R6F8W2R6_DANRE
TEK tyrosine kinase, endothelial
tek tie2
1,100Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053632 mRNA Translation: AAC09331.1
RefSeqiNP_571536.1, NM_131461.1
UniGeneiDr.75820

Genome annotation databases

GeneIDi30747
KEGGidre:30747

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053632 mRNA Translation: AAC09331.1
RefSeqiNP_571536.1, NM_131461.1
UniGeneiDr.75820

3D structure databases

ProteinModelPortaliO73791
SMRiO73791
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000055680

Proteomic databases

PaxDbiO73791
PRIDEiO73791

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi30747
KEGGidre:30747

Organism-specific databases

CTDi7010
ZFINiZDB-GENE-990415-56 tek

Phylogenomic databases

eggNOGiKOG0200 Eukaryota
COG0515 LUCA
HOGENOMiHOG000049232
HOVERGENiHBG007316
InParanoidiO73791
KOiK05121
PhylomeDBiO73791

Enzyme and pathway databases

BRENDAi2.7.10.1 928

Miscellaneous databases

PROiPR:O73791

Family and domain databases

CDDicd00063 FN3, 2 hits
Gene3Di2.60.40.10, 6 hits
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR018941 Tyr_kin_Tie2_Ig-like_dom-1_N
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF00041 fn3, 2 hits
PF07679 I-set, 1 hit
PF10430 Ig_Tie2_1, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00060 FN3, 3 hits
SM00409 IG, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
SSF49265 SSF49265, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 1 hit
PS50853 FN3, 3 hits
PS50835 IG_LIKE, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTIE2_DANRE
AccessioniPrimary (citable) accession number: O73791
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: August 1, 1998
Last modified: September 12, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again