UniProtKB - O73556 (POLG_HPE2W)
Protein
Genome polyprotein
Gene
N/A
Organism
Human parechovirus 2 (strain Williamson) (HPeV-2) (Echovirus 23)
Status
Functioni
Is not a protease.By similarity
Affects membrane integrity and cause an increase in membrane permeability.By similarity
Associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).By similarity
Protein 3A, via its hydrophobic domain, serves as membrane anchor.By similarity
cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind cooperatively to the protease (By similarity).By similarity
RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals.PROSITE-ProRule annotation
Catalytic activityi
- Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein. EC:3.4.22.29
- Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.PROSITE-ProRule annotation EC:3.4.22.28
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1557 | For protease 3C activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1595 | For protease 3C activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1669 | For protease 3C activityPROSITE-ProRule annotation | 1 | |
Active sitei | 2044 | For RdRp activityBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 1184 – 1191 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cysteine-type endopeptidase activity Source: UniProtKB-EC
- ion channel activity Source: UniProtKB-KW
- RNA binding Source: UniProtKB-KW
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- RNA helicase activity Source: InterPro
- structural molecule activity Source: InterPro
GO - Biological processi
- induction by virus of host autophagy Source: UniProtKB
- pore formation by virus in membrane of host cell Source: UniProtKB-KW
- protein complex oligomerization Source: UniProtKB-KW
- RNA-protein covalent cross-linking Source: UniProtKB-KW
- suppression by virus of host translation initiation factor activity Source: UniProtKB
- transcription, DNA-templated Source: InterPro
- viral entry into host cell Source: UniProtKB-KW
- viral RNA genome replication Source: InterPro
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Molecular function | Helicase, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-binding, RNA-directed RNA polymerase, Thiol protease, Transferase, Viral ion channel |
Biological process | Host-virus interaction, Ion transport, Transport, Viral attachment to host cell, Viral RNA replication, Virus entry into host cell |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Genome polyproteinCleaved into the following 10 chains: Alternative name(s): P1AB Virion protein 0 Alternative name(s): P1C Virion protein 3 Alternative name(s): P1D Virion protein 1 Alternative name(s): VPg Alternative name(s): Picornain 3C |
Organismi | Human parechovirus 2 (strain Williamson) (HPeV-2) (Echovirus 23) |
Taxonomic identifieri | 122962 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Picornavirales › Picornaviridae › Parechovirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion By similarity
- Host cytoplasm Curated
- Virion By similarity
- Host cytoplasm Curated
- Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity).By similarity
- Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity).By similarity
- Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity).By similarity
- Virion Curated
- Host cytoplasm Curated
- Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity).By similarity
GO - Cellular componenti
- host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
- integral to membrane of host cell Source: UniProtKB-KW
- membrane Source: UniProtKB-KW
- T=pseudo3 icosahedral viral capsid Source: UniProtKB-KW
Keywords - Cellular componenti
Capsid protein, Host cytoplasm, Host cytoplasmic vesicle, Host membrane, Membrane, T=pseudo3 icosahedral capsid protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000039750 | 1 – 289 | Protein VP0Sequence analysisAdd BLAST | 289 | |
ChainiPRO_0000039751 | 290 – 542 | Protein VP3Sequence analysisAdd BLAST | 253 | |
ChainiPRO_0000039752 | 543 – 775 | Protein VP1Sequence analysisAdd BLAST | 233 | |
ChainiPRO_0000039753 | 776 – 922 | Protein 2ASequence analysisAdd BLAST | 147 | |
ChainiPRO_0000039754 | 923 – 1044 | Protein 2BSequence analysisAdd BLAST | 122 | |
ChainiPRO_0000039755 | 1045 – 1373 | Protein 2CSequence analysisAdd BLAST | 329 | |
ChainiPRO_0000039756 | 1374 – 1490 | Protein 3ASequence analysisAdd BLAST | 117 | |
ChainiPRO_0000039757 | 1491 – 1510 | Protein 3BSequence analysisAdd BLAST | 20 | |
ChainiPRO_0000039758 | 1511 – 1710 | Protease 3CSequence analysisAdd BLAST | 200 | |
ChainiPRO_0000039759 | 1711 – 2179 | RNA-directed RNA polymerase 3D-POLSequence analysisAdd BLAST | 469 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1493 | O-(5'-phospho-RNA)-tyrosineBy similarity | 1 |
Post-translational modificationi
VPg is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity
Specific enzymatic cleavages yield mature proteins. All cleavages are catalyzed by P3C.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 775 – 776 | Cleavage; by protease 2ASequence analysis | 2 |
Keywords - PTMi
Covalent protein-RNA linkage, Lipoprotein, Myristate, PhosphoproteinProteomic databases
PRIDEi | O73556 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 786 – 881 | LRATPROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 1156 – 1317 | SF3 helicasePROSITE-ProRule annotationAdd BLAST | 162 | |
Domaini | 1517 – 1707 | Peptidase C3PROSITE-ProRule annotationAdd BLAST | 191 | |
Domaini | 1944 – 2058 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 115 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 763 – 765 | Cell attachment siteSequence analysis | 3 |
Sequence similaritiesi
Belongs to the picornaviruses polyprotein family.Curated
Family and domain databases
CDDi | cd00205, rhv_like, 2 hits |
Gene3Di | 2.40.10.10, 1 hit 2.60.120.20, 3 hits 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR004004, Helic/Pol/Pept_Calicivir-typ IPR000605, Helicase_SF3_ssDNA/RNA_vir IPR014759, Helicase_SF3_ssRNA_vir IPR027417, P-loop_NTPase IPR044067, PCV_3C_PRO IPR000199, Peptidase_C3A/C3B_picornavir IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001676, Picornavirus_capsid IPR043128, Rev_trsase/Diguanyl_cyclase IPR033703, Rhv-like IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus IPR029053, Viral_coat IPR009419, VPP_parechovir_P3A IPR009407, VPP_parechovir_P3B |
Pfami | View protein in Pfam PF06344, Parecho_VpG, 1 hit PF00548, Peptidase_C3, 1 hit PF06363, Picorna_P3A, 1 hit PF00680, RdRP_1, 1 hit PF00073, Rhv, 1 hit PF00910, RNA_helicase, 1 hit |
PRINTSi | PR00918, CALICVIRUSNS |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51934, LRAT, 1 hit PS51874, PCV_3C_PRO, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit PS51218, SF3_HELICASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O73556-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METIKSIADM ATGVTKTIDA TINSVNEIIT NTDNASGGDI LTKVADDASN
60 70 80 90 100
ILGPNCYATT SEPENKDVVQ ATTTVNTTNL TQHPSAPTLP FTPDFSNVDT
110 120 130 140 150
FHSMAYDTTT GSKNPNKLVR LTTHAWASTL QRGHQIDHVN LPVDFWDEQR
160 170 180 190 200
KPAYGHAKYF AAVRCGFHFQ VQVNVNQGTA GSALVVYEPK PVVDYDKDLE
210 220 230 240 250
FGAFTNLPHV LMNLAETTQA DLCIPYVADT NYVKTDSSDL GQLKVYVWTP
260 270 280 290 300
LSIPSGSSNQ VDVTILGSLL QLDFQNPRVY GQNVDIYDTA PSKPIPLRKT
310 320 330 340 350
KYLTMSTKYK WTRNKVDIAE GPGSMNMANV LSTTAAQSVA LVGERAFYDP
360 370 380 390 400
RTAGSKSRFD DLVKISQLFS VMADSTTPSA NHGIDQKGYF KWSANSDPQA
410 420 430 440 450
IVHRNLVHLN LFPNLKVFEN SYSYFRGSLI IRLSVYASTF NRGRLNGFFP
460 470 480 490 500
NSSTDETSEI DNAIYTICDI GSDNSFEITI PYSFSTWMRK THGKPIGLFQ
510 520 530 540 550
IEVLNRLTYN YSSPNEVYCI VQGKMGQDAK FFCPTGSLVT FQNSWGSQMD
560 570 580 590 600
LTDPLCIEDS VEDCKQTITP TELGLTSAQD DGPLGNDKPN YFLNFKSMNV
610 620 630 640 650
DIFTVSHTKV DNIFGRAWFA HVHDFTNDGL WRQGLEFPKE GHGALSLLFA
660 670 680 690 700
YFTGELNIHV LFLSDRGFLR VGHTYDTETN RTNFLSSSGI ITVPAGEQMT
710 720 730 740 750
LSVPSYSNKP LRTVRSSNAL GYLLCKPLLT GTSSGRIEIF LSLRCPNFFF
760 770 780 790 800
PLPAPKPATR KYRGDLATWS DQSPYGRQGK KQLMKLAYLD RGFYKHYGIV
810 820 830 840 850
VGDDVYQLDS DDIFKTALTG KAKFTKTRLT PDWVVEEECE LDYFRIKYLE
860 870 880 890 900
SSVNSEHIFS VDNNCETIAK DIFGSHSLSQ HQQIGLIGTI LLTAGLMSTI
910 920 930 940 950
KTPVNPTTIK EFFNHAIEGD EQGLSLLVQK CTTFFSSAAT ELLDNDLVKF
960 970 980 990 1000
IIKILVRILC YMVLYCHKPN ILTTACLSTL LVMDVTSSSV LSPSCKALMQ
1010 1020 1030 1040 1050
CLMDGDVKKL AEVVAESMSN TDDDEIKEQI CDTVKYTKQI LSNQGPFKGF
1060 1070 1080 1090 1100
NEISTAFRHI DWWIQTLLKI KDMVLSVFKP SVEKRAVEWL ERNKEHVCSI
1110 1120 1130 1140 1150
LDYASDIIVK SKDQTKMKTQ EFYQRYNDCL SKFKPIMAMC FRSCHNSISN
1160 1170 1180 1190 1200
TVYRLFQELA RIPNRMATQN DLIRVEPIGI WIQGEPGQGK SFLTHTLSKQ
1210 1220 1230 1240 1250
LQKTCGLQGI YTNPTASEFM DGYDNQDIHL IDDLGQTRKE RDIEMLCNCI
1260 1270 1280 1290 1300
SSDPDIVPMA HLEEKGKFYT SKLVIATTNK PDFSSTVLLD SGALRRRFPY
1310 1320 1330 1340 1350
IMHIRAAKHY SKSGKLNVSQ AMPHMSTGEC WEVSKNGRDW ETLKLKELID
1360 1370 1380 1390 1400
KITVDYKERI ANYNTWKKQL EDQTLDDLDD AVSYIKHNYP DAIPYIDEYL
1410 1420 1430 1440 1450
NIEMSTLIEQ MEAFIEPKPS VFKCFASRVG DKIKEASREV VKWFSDKLKS
1460 1470 1480 1490 1500
MLNFVERNKA WLTVVSAVTS AIGILLLVTK IFKKEESKDE RAYNPTLPVA
1510 1520 1530 1540 1550
KPKGTFPVSQ REFKNEAPYD GQLEHIISQM AYITGSTTGH ITHCAGYQHD
1560 1570 1580 1590 1600
EIILHGHSIK YLEQEEELTL HYKNKVFPIE QPSVTQVTLG GKPMDLAIVK
1610 1620 1630 1640 1650
CKLPFRFKKN SKYYTNKIGT ESMLIWMTEQ GIITKEVQRV HHSGGIKTRE
1660 1670 1680 1690 1700
GTESTKTISY TVKSCKGMCG GLLISKVEGN FKILGMHIAG NGEMGVAIPF
1710 1720 1730 1740 1750
NFLKNDMSDQ GIVTEVTPIQ PMYINTKSQI HKSPVYGAVE VKMGPAVLSK
1760 1770 1780 1790 1800
SDTRLEEPVD CLVKKSASKY RVNKFQVNNE LWQGVKACVK SKFREIFGVN
1810 1820 1830 1840 1850
GIVDMKTAIL GTSHVNSMDL STSAGYSFVK SGYKKKDLIC LEPFSVSPML
1860 1870 1880 1890 1900
EKLVQEKFHN LLKGNQITTI FNTCLKDELR KLDKIATGKT RCIEACEIDY
1910 1920 1930 1940 1950
CIVYRMIMME IYDKIYQTPC YYSGLAVGIN PYRDWHFMIN ALNDYNYEMD
1960 1970 1980 1990 2000
YSQYDGSLSS MLLWEAVQVL AYCHDSPDLV MQLHKPVIDS DHVVFNERWL
2010 2020 2030 2040 2050
IHGGMPSGSP CTTVLNSLCN LMMCIYTTNL ISPGIDCLPI VYGDDVILSL
2060 2070 2080 2090 2100
DKEIEPERLQ SIMAESFGAE VTGSRKDEPP SLKPRMEVEF LKRKPGYFPE
2110 2120 2130 2140 2150
STFIVGKLDT ENMIQHLMWM KNFSTFKQQL QSYLMELCLH GKDTYQHYVK
2160 2170
ILNPYLKEWN IPVDDYEVVI GKLVPMVFD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005695 Genomic RNA Translation: CAA06679.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005695 Genomic RNA Translation: CAA06679.1 |
3D structure databases
SMRi | O73556 |
ModBasei | Search... |
Proteomic databases
PRIDEi | O73556 |
Family and domain databases
CDDi | cd00205, rhv_like, 2 hits |
Gene3Di | 2.40.10.10, 1 hit 2.60.120.20, 3 hits 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR043502, DNA/RNA_pol_sf IPR004004, Helic/Pol/Pept_Calicivir-typ IPR000605, Helicase_SF3_ssDNA/RNA_vir IPR014759, Helicase_SF3_ssRNA_vir IPR027417, P-loop_NTPase IPR044067, PCV_3C_PRO IPR000199, Peptidase_C3A/C3B_picornavir IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001676, Picornavirus_capsid IPR043128, Rev_trsase/Diguanyl_cyclase IPR033703, Rhv-like IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus IPR029053, Viral_coat IPR009419, VPP_parechovir_P3A IPR009407, VPP_parechovir_P3B |
Pfami | View protein in Pfam PF06344, Parecho_VpG, 1 hit PF00548, Peptidase_C3, 1 hit PF06363, Picorna_P3A, 1 hit PF00680, RdRP_1, 1 hit PF00073, Rhv, 1 hit PF00910, RNA_helicase, 1 hit |
PRINTSi | PR00918, CALICVIRUSNS |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51934, LRAT, 1 hit PS51874, PCV_3C_PRO, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit PS51218, SF3_HELICASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | POLG_HPE2W | |
Accessioni | O73556Primary (citable) accession number: O73556 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | August 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families