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Protein

Radical S-adenosyl methionine domain-containing protein 2

Gene

Rsad2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interferon-inducible iron-sulfur (4FE-4S) cluster-binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of viruses, including west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus and vesicular stomatitis virus (VSV). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphosphate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cell activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins (By similarity).By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi82Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi86Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi89Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAntiviral defense, Immunity, Innate immunity
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Radical S-adenosyl methionine domain-containing protein 2
Alternative name(s):
Bone-expressed sequence tag 5 protein
Viperin
Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible
Gene namesi
Name:Rsad2By similarity
Synonyms:Best5Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620495 Rsad2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003095861 – 360Radical S-adenosyl methionine domain-containing protein 2Add BLAST360

Proteomic databases

PaxDbiO70600
PRIDEiO70600

Expressioni

Tissue specificityi

In neonatal rat tibia, specifically localized in cells of the periosteum, in osteoblasts lining endosteal and peristeal bone surfaces, to articular surfaces of cartilage and in perichondral cells but not in chondrocytes (at protein level). Expressed predominantly in bone marrow and spleen.1 Publication

Developmental stagei

Not detected in undifferentiated primary osteoblasts. Expression increases during differentiation and declines to much reduced levels in mature osteoblasts.1 Publication

Inductioni

By interferon type I, type II and LPS. Interferon alpha induction is rapid and transient, peaks 4-6 hours after stimulation and returns to basal levels 24 hours after stimulation. Interferon gamma elicits a more prolonged response where expression remains elevated 48 hours after stimulation. Induced by infection with Vesicular stomatitis virus and pseudorabies virus, presumably through type I interferon pathway.1 Publication

Gene expression databases

GenevisibleiO70600 RN

Interactioni

Subunit structurei

Homodimer. Interacts (via C-terminus) with VAPA/VAP33 (via C-terminus) and inhibits its interaction with hepatitis virus C (HCV) protein NS5A. Interacts with HADHB, IRAK1 and TRAF6 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010092

Structurei

3D structure databases

ProteinModelPortaliO70600
SMRiO70600
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region (1-41) is necessary for its localization to the endoplasmic reticulum membrane and lipid droplet.By similarity

Sequence similaritiesi

Belongs to the radical SAM superfamily. RSAD2 family.Curated

Phylogenomic databases

eggNOGiENOG410IGZE Eukaryota
ENOG410XQM5 LUCA
HOGENOMiHOG000194793
HOVERGENiHBG053099
InParanoidiO70600
KOiK15045
OrthoDBiEOG091G0GXY
PhylomeDBiO70600

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
IPR034395 SPASM/twitch_domain-containing
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01067 SPASM/twitch_domain_containing, 1 hit
SFLDS00029 Radical_SAM, 2 hits
SMARTiView protein in SMART
SM00729 Elp3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O70600-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLVPTALAAR LLSLFQQQLG SLWSGLAMLF CWLRIALGWP DPGKGQPRVR
60 70 80 90 100
GEPKETQETH EDPGSAQPTT PVSVNYHFTR QCNYKCGFCF HTAKTSFVLP
110 120 130 140 150
LEEAKRGLLL LKQAGMEKIN FSGGEPFLQD RGEYLGKLVR FCKEELALPS
160 170 180 190 200
VSIVSNGSLI RERWFKDYGD YLDILAISCD SFDEQVNVLI GRGQGKKNHV
210 220 230 240 250
ENLQKLRKWC RDYKVAFKIN SVINRFNVDE DMNEHIKALS PVRWKVFQCL
260 270 280 290 300
LIEGENSGED ALREAERFLI SNEEFEAFLQ RHKDVSCLVP ESNQKMKDSY
310 320 330 340 350
LILDEYMRFL NCTGGRKDPS RSILDVGVEE AIKFSGFDEK MFLKRGGKYV
360
WSKADLKLDW
Length:360
Mass (Da):41,255
Last modified:August 1, 1998 - v1
Checksum:i4947AF2A286CB500
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0H2UHF4A0A0H2UHF4_RAT
RCG62278
Rsad2 rCG_62278
362Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07704 mRNA Translation: CAA68971.1
RefSeqiNP_620236.1, NM_138881.1
UniGeneiRn.14882

Genome annotation databases

GeneIDi65190
KEGGirno:65190

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07704 mRNA Translation: CAA68971.1
RefSeqiNP_620236.1, NM_138881.1
UniGeneiRn.14882

3D structure databases

ProteinModelPortaliO70600
SMRiO70600
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010092

Proteomic databases

PaxDbiO70600
PRIDEiO70600

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65190
KEGGirno:65190

Organism-specific databases

CTDi91543
RGDi620495 Rsad2

Phylogenomic databases

eggNOGiENOG410IGZE Eukaryota
ENOG410XQM5 LUCA
HOGENOMiHOG000194793
HOVERGENiHBG053099
InParanoidiO70600
KOiK15045
OrthoDBiEOG091G0GXY
PhylomeDBiO70600

Miscellaneous databases

PROiPR:O70600

Gene expression databases

GenevisibleiO70600 RN

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
IPR034395 SPASM/twitch_domain-containing
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01067 SPASM/twitch_domain_containing, 1 hit
SFLDS00029 Radical_SAM, 2 hits
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRSAD2_RAT
AccessioniPrimary (citable) accession number: O70600
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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