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Protein

E3 ubiquitin-protein ligase Midline-1

Gene

Mid1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi119Zinc 1By similarity1
Metal bindingi122Zinc 1By similarity1
Metal bindingi134Zinc 2By similarity1
Metal bindingi137Zinc 2By similarity1
Metal bindingi142Zinc 1By similarity1
Metal bindingi145Zinc 1By similarity1
Metal bindingi150Zinc 2By similarity1
Metal bindingi159Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 60RING-typePROSITE-ProRule annotationAdd BLAST51
Zinc fingeri116 – 165B box-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri172 – 212B box-type 2PROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Midline-1 (EC:2.3.2.27)
Alternative name(s):
Midin
RING finger protein Midline-1
RING-type E3 ubiquitin transferase Midline-1Curated
Tripartite motif-containing protein 18
Gene namesi
Name:Mid1
Synonyms:Fxy, Trim18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1100537 Mid1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562281 – 680E3 ubiquitin-protein ligase Midline-1Add BLAST680

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei92PhosphoserineBy similarity1
Modified residuei96PhosphoserineBy similarity1
Modified residuei524PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO70583
PaxDbiO70583
PRIDEiO70583

PTM databases

iPTMnetiO70583
PhosphoSitePlusiO70583

Expressioni

Tissue specificityi

Ubiquitously expressed in fetus and adult. At E9-E10.5, highest expression found in frontonasal processes, branchial arches and CNS. From E12.5 to E16.5, high levels found in rostral part of CNS. At E14.5, begins to be highly expressed in kidney and lung. At E16.5, highly expressed in the mucosa of the hindgut and cutaneous region of the stomach.1 Publication

Developmental stagei

Expressed throughout embryonic development with highest levels from E7-E11. Also expressed in the adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000035299 Expressed in 196 organ(s), highest expression level in head
CleanExiMM_MID1
ExpressionAtlasiO70583 baseline and differential
GenevisibleiO70583 MM

Interactioni

Subunit structurei

Homodimer or heterodimer with MID2. Interacts with IGBP1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Mid1ip1Q9CQ208EBI-472994,EBI-473024

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201417, 2 interactors
IntActiO70583, 2 interactors
MINTiO70583
STRINGi10090.ENSMUSP00000038765

Structurei

3D structure databases

ProteinModelPortaliO70583
SMRiO70583
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini320 – 379COSPROSITE-ProRule annotationAdd BLAST60
Domaini384 – 494Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST111
Domaini495 – 672B30.2/SPRYPROSITE-ProRule annotationAdd BLAST178

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili205 – 264Sequence analysisAdd BLAST60

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 60RING-typePROSITE-ProRule annotationAdd BLAST51
Zinc fingeri116 – 165B box-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri172 – 212B box-type 2PROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG4111G04 LUCA
GeneTreeiENSGT00760000118878
HOGENOMiHOG000049193
HOVERGENiHBG056432
InParanoidiO70583
KOiK08285
OMAiCRCHNNW
OrthoDBiEOG091G02K9
TreeFamiTF333654

Family and domain databases

CDDicd00021 BBOX, 1 hit
cd00063 FN3, 1 hit
Gene3Di2.60.40.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003649 Bbox_C
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR017903 COS_domain
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR027727 MID1
IPR006574 PRY
IPR003877 SPRY_dom
IPR027370 Znf-RING_LisH
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR44073:SF3 PTHR44073:SF3, 1 hit
PfamiView protein in Pfam
PF00041 fn3, 1 hit
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PF13445 zf-RING_UBOX, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00502 BBC, 1 hit
SM00336 BBOX, 2 hits
SM00060 FN3, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49265 SSF49265, 1 hit
SSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS51262 COS, 1 hit
PS50853 FN3, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O70583-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNEPVES
60 70 80 90 100
INAFQCPTCR HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET
110 120 130 140 150
RRERAFDANT MSSAEKVLCQ FCDQDPAQDA VKTCVTCEVS YCDECLKATH
160 170 180 190 200
PNKKPFTGHR LIEPIPDSHI RGLMCLEHED EKVNMYCVTD DQLICALCKL
210 220 230 240 250
VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL LAKLIQTCQH
260 270 280 290 300
VEVNASRQEA KLTEECDLLI EIIQQRRQII GTKIKEGKVI RLRKLAQQIA
310 320 330 340 350
NCKQCLERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI
360 370 380 390 400
PEINLNDTFD TFALDFSREK KLLECLDYLT APNPPAIREE LCTASYDTIT
410 420 430 440 450
VHWTSEDEFS VVSYELQYTI FTGQANVVNV ACDGTCLLGS AGLCNSADSW
460 470 480 490 500
MIVPNIKQNH YTVHGLQSGT KYIFTVKAIN QAGSRSSEPG KLKTNSQPFR
510 520 530 540 550
LDPKSAHRKL KVSHDNLTVE RDESSSKKSH APERFAGQGS YGVAGNVFID
560 570 580 590 600
SGRHYWEVVT SGSTWYAIGL AYRSAPKHEW IGKNAASWAL CRCHNHWAVR
610 620 630 640 650
HDGKETPIAP APHLRRVGVL LDYDNGSIAF YDALSSVHLH TFHAALAQPV
660 670 680
CPTFTVWNKC LTIVTGLPIP DHLDCTEQRP
Length:680
Mass (Da):76,136
Last modified:July 27, 2011 - v2
Checksum:i47D8551531157308
GO
Isoform 2 (identifier: O70583-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-442: NVACDGTCLLGSAG → S

Show »
Length:667
Mass (Da):74,960
Checksum:i0844933089B553D3
GO
Isoform 3 (identifier: O70583-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     183-220: Missing.
     429-442: NVACDGTCLLGSAG → S

Note: No experimental confirmation available.
Show »
Length:629
Mass (Da):70,572
Checksum:i1C4C2BD6B092B25E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AV01B1AV01_MOUSE
E3 ubiquitin-protein ligase Midline...
Mid1
642Annotation score:
Q6PD02Q6PD02_MOUSE
E3 ubiquitin-protein ligase Midline...
Mid1
400Annotation score:
B1AUZ9B1AUZ9_MOUSE
E3 ubiquitin-protein ligase Midline...
Mid1
474Annotation score:

Sequence cautioni

The sequence AAH53704 differs from that shown. Reason: Erroneous termination at position 497. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65L → P in AAH53704 (PubMed:15489334).Curated1
Sequence conflicti109N → D in AAH53704 (PubMed:15489334).Curated1
Sequence conflicti174M → T in AAB83986 (PubMed:9342357).Curated1
Sequence conflicti464H → Y in AAH53704 (PubMed:15489334).Curated1
Sequence conflicti523E → D in CAA75113 (PubMed:9467009).Curated1
Sequence conflicti633A → T in AAB83986 (PubMed:9342357).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010811183 – 220Missing in isoform 3. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_005736429 – 442NVACD…LGSAG → S in isoform 2 and isoform 3. 3 PublicationsAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14848 mRNA Translation: CAA75113.1
AF026565 mRNA Translation: AAB83986.1
AL672015, CR478112 Genomic DNA Translation: CAM15638.1
CR478112, AL672015 Genomic DNA Translation: CAM28195.1
BC053704 mRNA Translation: AAH53704.1 Sequence problems.
CCDSiCCDS41215.1 [O70583-1]
PIRiT09013
RefSeqiNP_001277433.1, NM_001290504.1 [O70583-2]
NP_001277434.1, NM_001290505.1 [O70583-2]
NP_001277435.1, NM_001290506.1 [O70583-3]
NP_034927.2, NM_010797.3 [O70583-1]
XP_011246091.1, XM_011247789.2 [O70583-2]
XP_017173897.1, XM_017318408.1 [O70583-1]
XP_017173898.1, XM_017318409.1 [O70583-1]
XP_017173899.1, XM_017318410.1 [O70583-1]
UniGeneiMm.34441

Genome annotation databases

EnsembliENSMUST00000036753; ENSMUSP00000038765; ENSMUSG00000035299 [O70583-1]
ENSMUST00000112104; ENSMUSP00000107732; ENSMUSG00000035299 [O70583-1]
ENSMUST00000112105; ENSMUSP00000107733; ENSMUSG00000035299 [O70583-1]
ENSMUST00000163810; ENSMUSP00000128176; ENSMUSG00000035299 [O70583-1]
ENSMUST00000171433; ENSMUSP00000126746; ENSMUSG00000035299 [O70583-1]
GeneIDi17318
KEGGimmu:17318
UCSCiuc009uya.2 mouse [O70583-1]
uc009uyc.2 mouse [O70583-2]
uc057ats.1 mouse [O70583-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14848 mRNA Translation: CAA75113.1
AF026565 mRNA Translation: AAB83986.1
AL672015, CR478112 Genomic DNA Translation: CAM15638.1
CR478112, AL672015 Genomic DNA Translation: CAM28195.1
BC053704 mRNA Translation: AAH53704.1 Sequence problems.
CCDSiCCDS41215.1 [O70583-1]
PIRiT09013
RefSeqiNP_001277433.1, NM_001290504.1 [O70583-2]
NP_001277434.1, NM_001290505.1 [O70583-2]
NP_001277435.1, NM_001290506.1 [O70583-3]
NP_034927.2, NM_010797.3 [O70583-1]
XP_011246091.1, XM_011247789.2 [O70583-2]
XP_017173897.1, XM_017318408.1 [O70583-1]
XP_017173898.1, XM_017318409.1 [O70583-1]
XP_017173899.1, XM_017318410.1 [O70583-1]
UniGeneiMm.34441

3D structure databases

ProteinModelPortaliO70583
SMRiO70583
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201417, 2 interactors
IntActiO70583, 2 interactors
MINTiO70583
STRINGi10090.ENSMUSP00000038765

PTM databases

iPTMnetiO70583
PhosphoSitePlusiO70583

Proteomic databases

MaxQBiO70583
PaxDbiO70583
PRIDEiO70583

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036753; ENSMUSP00000038765; ENSMUSG00000035299 [O70583-1]
ENSMUST00000112104; ENSMUSP00000107732; ENSMUSG00000035299 [O70583-1]
ENSMUST00000112105; ENSMUSP00000107733; ENSMUSG00000035299 [O70583-1]
ENSMUST00000163810; ENSMUSP00000128176; ENSMUSG00000035299 [O70583-1]
ENSMUST00000171433; ENSMUSP00000126746; ENSMUSG00000035299 [O70583-1]
GeneIDi17318
KEGGimmu:17318
UCSCiuc009uya.2 mouse [O70583-1]
uc009uyc.2 mouse [O70583-2]
uc057ats.1 mouse [O70583-3]

Organism-specific databases

CTDi4281
MGIiMGI:1100537 Mid1

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG4111G04 LUCA
GeneTreeiENSGT00760000118878
HOGENOMiHOG000049193
HOVERGENiHBG056432
InParanoidiO70583
KOiK08285
OMAiCRCHNNW
OrthoDBiEOG091G02K9
TreeFamiTF333654

Miscellaneous databases

ChiTaRSiMid1 mouse
PROiPR:O70583
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035299 Expressed in 196 organ(s), highest expression level in head
CleanExiMM_MID1
ExpressionAtlasiO70583 baseline and differential
GenevisibleiO70583 MM

Family and domain databases

CDDicd00021 BBOX, 1 hit
cd00063 FN3, 1 hit
Gene3Di2.60.40.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003649 Bbox_C
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR017903 COS_domain
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR027727 MID1
IPR006574 PRY
IPR003877 SPRY_dom
IPR027370 Znf-RING_LisH
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR44073:SF3 PTHR44073:SF3, 1 hit
PfamiView protein in Pfam
PF00041 fn3, 1 hit
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PF13445 zf-RING_UBOX, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00502 BBC, 1 hit
SM00336 BBOX, 2 hits
SM00060 FN3, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49265 SSF49265, 1 hit
SSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS51262 COS, 1 hit
PS50853 FN3, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRI18_MOUSE
AccessioniPrimary (citable) accession number: O70583
Secondary accession number(s): B1AV00, O35418, Q7TPT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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