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Protein

E3 ubiquitin-protein ligase Midline-1

Gene

Mid1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi119Zinc 1By similarity1
Metal bindingi122Zinc 1By similarity1
Metal bindingi134Zinc 2By similarity1
Metal bindingi137Zinc 2By similarity1
Metal bindingi142Zinc 1By similarity1
Metal bindingi145Zinc 1By similarity1
Metal bindingi150Zinc 2By similarity1
Metal bindingi159Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri10 – 60RING-typePROSITE-ProRule annotationAdd BLAST51
Zinc fingeri116 – 165B box-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri172 – 212B box-type 2PROSITE-ProRule annotationAdd BLAST41

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Midline-1 (EC:2.3.2.27)
Alternative name(s):
Midin
RING finger protein Midline-1
RING-type E3 ubiquitin transferase Midline-1Curated
Tripartite motif-containing protein 18
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Mid1
Synonyms:Fxy, Trim18
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome X

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:1100537 Mid1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000562281 – 680E3 ubiquitin-protein ligase Midline-1Add BLAST680

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei92PhosphoserineBy similarity1
Modified residuei96PhosphoserineBy similarity1
Modified residuei524PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
O70583

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
O70583

PRoteomics IDEntifications database

More...
PRIDEi
O70583

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
O70583

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
O70583

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed in fetus and adult. At E9-E10.5, highest expression found in frontonasal processes, branchial arches and CNS. From E12.5 to E16.5, high levels found in rostral part of CNS. At E14.5, begins to be highly expressed in kidney and lung. At E16.5, highly expressed in the mucosa of the hindgut and cutaneous region of the stomach.1 Publication

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed throughout embryonic development with highest levels from E7-E11. Also expressed in the adult.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000035299 Expressed in 196 organ(s), highest expression level in head

CleanEx database of gene expression profiles

More...
CleanExi
MM_MID1

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
O70583 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
O70583 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer or heterodimer with MID2. Interacts with IGBP1.By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
Mid1ip1Q9CQ208EBI-472994,EBI-473024

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
201417, 2 interactors

Protein interaction database and analysis system

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IntActi
O70583, 2 interactors

Molecular INTeraction database

More...
MINTi
O70583

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000038765

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

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ProteinModelPortali
O70583

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
O70583

Database of comparative protein structure models

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ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini320 – 379COSPROSITE-ProRule annotationAdd BLAST60
Domaini384 – 494Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST111
Domaini495 – 672B30.2/SPRYPROSITE-ProRule annotationAdd BLAST178

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili205 – 264Sequence analysisAdd BLAST60

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 60RING-typePROSITE-ProRule annotationAdd BLAST51
Zinc fingeri116 – 165B box-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri172 – 212B box-type 2PROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2177 Eukaryota
ENOG4111G04 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000155821

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000049193

The HOVERGEN Database of Homologous Vertebrate Genes

More...
HOVERGENi
HBG056432

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O70583

KEGG Orthology (KO)

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KOi
K08285

Identification of Orthologs from Complete Genome Data

More...
OMAi
CRCHNNW

TreeFam database of animal gene trees

More...
TreeFami
TF333654

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00021 BBOX, 1 hit
cd00063 FN3, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.60.40.10, 1 hit
3.30.40.10, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR001870 B30.2/SPRY
IPR003649 Bbox_C
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR017903 COS_domain
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR027727 MID1
IPR006574 PRY
IPR003877 SPRY_dom
IPR027370 Znf-RING_LisH
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS

The PANTHER Classification System

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PANTHERi
PTHR44073:SF3 PTHR44073:SF3, 1 hit

Pfam protein domain database

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Pfami
View protein in Pfam
PF00041 fn3, 1 hit
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PF13445 zf-RING_UBOX, 1 hit

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR01407 BUTYPHLNCDUF

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00502 BBC, 1 hit
SM00336 BBOX, 2 hits
SM00060 FN3, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF49265 SSF49265, 1 hit
SSF49899 SSF49899, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS51262 COS, 1 hit
PS50853 FN3, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O70583-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNEPVES
60 70 80 90 100
INAFQCPTCR HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET
110 120 130 140 150
RRERAFDANT MSSAEKVLCQ FCDQDPAQDA VKTCVTCEVS YCDECLKATH
160 170 180 190 200
PNKKPFTGHR LIEPIPDSHI RGLMCLEHED EKVNMYCVTD DQLICALCKL
210 220 230 240 250
VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL LAKLIQTCQH
260 270 280 290 300
VEVNASRQEA KLTEECDLLI EIIQQRRQII GTKIKEGKVI RLRKLAQQIA
310 320 330 340 350
NCKQCLERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI
360 370 380 390 400
PEINLNDTFD TFALDFSREK KLLECLDYLT APNPPAIREE LCTASYDTIT
410 420 430 440 450
VHWTSEDEFS VVSYELQYTI FTGQANVVNV ACDGTCLLGS AGLCNSADSW
460 470 480 490 500
MIVPNIKQNH YTVHGLQSGT KYIFTVKAIN QAGSRSSEPG KLKTNSQPFR
510 520 530 540 550
LDPKSAHRKL KVSHDNLTVE RDESSSKKSH APERFAGQGS YGVAGNVFID
560 570 580 590 600
SGRHYWEVVT SGSTWYAIGL AYRSAPKHEW IGKNAASWAL CRCHNHWAVR
610 620 630 640 650
HDGKETPIAP APHLRRVGVL LDYDNGSIAF YDALSSVHLH TFHAALAQPV
660 670 680
CPTFTVWNKC LTIVTGLPIP DHLDCTEQRP
Length:680
Mass (Da):76,136
Last modified:July 27, 2011 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i47D8551531157308
GO
Isoform 2 (identifier: O70583-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-442: NVACDGTCLLGSAG → S

Show »
Length:667
Mass (Da):74,960
Checksum:i0844933089B553D3
GO
Isoform 3 (identifier: O70583-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     183-220: Missing.
     429-442: NVACDGTCLLGSAG → S

Note: No experimental confirmation available.
Show »
Length:629
Mass (Da):70,572
Checksum:i1C4C2BD6B092B25E
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AV01B1AV01_MOUSE
E3 ubiquitin-protein ligase Midline...
Mid1
642Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q6PD02Q6PD02_MOUSE
E3 ubiquitin-protein ligase Midline...
Mid1
400Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
B1AUZ9B1AUZ9_MOUSE
E3 ubiquitin-protein ligase Midline...
Mid1
474Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAH53704 differs from that shown. Reason: Erroneous termination at position 497. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti65L → P in AAH53704 (PubMed:15489334).Curated1
Sequence conflicti109N → D in AAH53704 (PubMed:15489334).Curated1
Sequence conflicti174M → T in AAB83986 (PubMed:9342357).Curated1
Sequence conflicti464H → Y in AAH53704 (PubMed:15489334).Curated1
Sequence conflicti523E → D in CAA75113 (PubMed:9467009).Curated1
Sequence conflicti633A → T in AAB83986 (PubMed:9342357).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting. The information stored in this subsection is used to automatically construct alternative protein sequence(s) for display.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_010811183 – 220Missing in isoform 3. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_005736429 – 442NVACD…LGSAG → S in isoform 2 and isoform 3. 3 PublicationsAdd BLAST14

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
Y14848 mRNA Translation: CAA75113.1
AF026565 mRNA Translation: AAB83986.1
AL672015, CR478112 Genomic DNA Translation: CAM15638.1
CR478112, AL672015 Genomic DNA Translation: CAM28195.1
BC053704 mRNA Translation: AAH53704.1 Sequence problems.

The Consensus CDS (CCDS) project

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CCDSi
CCDS41215.1 [O70583-1]

Protein sequence database of the Protein Information Resource

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PIRi
T09013

NCBI Reference Sequences

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RefSeqi
NP_001277433.1, NM_001290504.1 [O70583-2]
NP_001277434.1, NM_001290505.1 [O70583-2]
NP_001277435.1, NM_001290506.1 [O70583-3]
NP_034927.2, NM_010797.3 [O70583-1]
XP_011246091.1, XM_011247789.2 [O70583-2]
XP_017173897.1, XM_017318408.1 [O70583-1]
XP_017173898.1, XM_017318409.1 [O70583-1]
XP_017173899.1, XM_017318410.1 [O70583-1]

UniGene gene-oriented nucleotide sequence clusters

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UniGenei
Mm.34441

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENSMUST00000036753; ENSMUSP00000038765; ENSMUSG00000035299 [O70583-1]
ENSMUST00000112104; ENSMUSP00000107732; ENSMUSG00000035299 [O70583-1]
ENSMUST00000112105; ENSMUSP00000107733; ENSMUSG00000035299 [O70583-1]
ENSMUST00000163810; ENSMUSP00000128176; ENSMUSG00000035299 [O70583-1]
ENSMUST00000171433; ENSMUSP00000126746; ENSMUSG00000035299 [O70583-1]

Database of genes from NCBI RefSeq genomes

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GeneIDi
17318

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
mmu:17318

UCSC genome browser

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UCSCi
uc009uya.2 mouse [O70583-1]
uc009uyc.2 mouse [O70583-2]
uc057ats.1 mouse [O70583-3]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14848 mRNA Translation: CAA75113.1
AF026565 mRNA Translation: AAB83986.1
AL672015, CR478112 Genomic DNA Translation: CAM15638.1
CR478112, AL672015 Genomic DNA Translation: CAM28195.1
BC053704 mRNA Translation: AAH53704.1 Sequence problems.
CCDSiCCDS41215.1 [O70583-1]
PIRiT09013
RefSeqiNP_001277433.1, NM_001290504.1 [O70583-2]
NP_001277434.1, NM_001290505.1 [O70583-2]
NP_001277435.1, NM_001290506.1 [O70583-3]
NP_034927.2, NM_010797.3 [O70583-1]
XP_011246091.1, XM_011247789.2 [O70583-2]
XP_017173897.1, XM_017318408.1 [O70583-1]
XP_017173898.1, XM_017318409.1 [O70583-1]
XP_017173899.1, XM_017318410.1 [O70583-1]
UniGeneiMm.34441

3D structure databases

ProteinModelPortaliO70583
SMRiO70583
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201417, 2 interactors
IntActiO70583, 2 interactors
MINTiO70583
STRINGi10090.ENSMUSP00000038765

PTM databases

iPTMnetiO70583
PhosphoSitePlusiO70583

Proteomic databases

MaxQBiO70583
PaxDbiO70583
PRIDEiO70583

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036753; ENSMUSP00000038765; ENSMUSG00000035299 [O70583-1]
ENSMUST00000112104; ENSMUSP00000107732; ENSMUSG00000035299 [O70583-1]
ENSMUST00000112105; ENSMUSP00000107733; ENSMUSG00000035299 [O70583-1]
ENSMUST00000163810; ENSMUSP00000128176; ENSMUSG00000035299 [O70583-1]
ENSMUST00000171433; ENSMUSP00000126746; ENSMUSG00000035299 [O70583-1]
GeneIDi17318
KEGGimmu:17318
UCSCiuc009uya.2 mouse [O70583-1]
uc009uyc.2 mouse [O70583-2]
uc057ats.1 mouse [O70583-3]

Organism-specific databases

Comparative Toxicogenomics Database

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CTDi
4281
MGIiMGI:1100537 Mid1

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG4111G04 LUCA
GeneTreeiENSGT00940000155821
HOGENOMiHOG000049193
HOVERGENiHBG056432
InParanoidiO70583
KOiK08285
OMAiCRCHNNW
TreeFamiTF333654

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

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ChiTaRSi
Mid1 mouse

Protein Ontology

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PROi
PR:O70583

The Stanford Online Universal Resource for Clones and ESTs

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SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000035299 Expressed in 196 organ(s), highest expression level in head
CleanExiMM_MID1
ExpressionAtlasiO70583 baseline and differential
GenevisibleiO70583 MM

Family and domain databases

CDDicd00021 BBOX, 1 hit
cd00063 FN3, 1 hit
Gene3Di2.60.40.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003649 Bbox_C
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR017903 COS_domain
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR027727 MID1
IPR006574 PRY
IPR003877 SPRY_dom
IPR027370 Znf-RING_LisH
IPR000315 Znf_B-box
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR44073:SF3 PTHR44073:SF3, 1 hit
PfamiView protein in Pfam
PF00041 fn3, 1 hit
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PF00643 zf-B_box, 1 hit
PF13445 zf-RING_UBOX, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00502 BBC, 1 hit
SM00336 BBOX, 2 hits
SM00060 FN3, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49265 SSF49265, 1 hit
SSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS51262 COS, 1 hit
PS50853 FN3, 1 hit
PS50119 ZF_BBOX, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTRI18_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O70583
Secondary accession number(s): B1AV00, O35418, Q7TPT6
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: July 27, 2011
Last modified: January 16, 2019
This is version 173 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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