UniProtKB - O70551 (SRPK1_MOUSE)

BLAST

>sp|O70551|SRPK1_MOUSE SRSF protein kinase 1 OS=Mus musculus OX=10090 GN=Srpk1 PE=1 SV=2
MERKVLALQARKKRTKAKKDKAQRKPETQHRGSAPHSESDIPEQEEEILGSDDDEQEDPN
DYCKGGYHLVKIGDLFNGRYHVIRKLGWGHFSTVWLSWDIQGKKFVAMKVVKSAEHYTET
ALDEIRLLKSVRNSDPNDPNGEMVVQLLDDFKISGVNGTHICMVFEVLGHHLLKWIIKSN
YQGLPLPCVKKIIQQVLQGLDYLHTKCRIIHTDIKPENILLSVNEQYIRRLAAEATEWQR
SGAPPPSGSAVSTAPQPKPADKMSKNKKKKLKKKQKRQAELLEKRMQEIEEMEKESGPGQ
KRPNKQEESESPVDRPLTENPPNKMTQEKLEESNSIGQDQTLTERGGEGGAPEINCNGVI
GVVNYPENSNNETLRHKEDLHNANDCDVHTLKQEPSFLNSSNGDSSPSQDTDSCTPTASE
TMVCQSSAEQSLTRQDITQLEESIRADTPSGDEQEPNGALDSKGKFSAGNFLINPLEPKN
AEKLQVKIADLGNACWVHKHFTEDIQTRQYRSLEVLIGSGYNTPADIWSTACMAFELATG
DYLFEPHSGEDYTRDEDHIALIIELLGKVPRKLIVAGKYSKEFFTKKGDLKHITKLKPWG
LLEVLVEKYEWPQEEAAGFTDFLLPMLELMPEKRATAAECLRHPWLNS

Align
Format
Add to basket Added to basket
History
Entry version 158 (12 Sep 2018)
Sequence version 2 (21 Jun 2005)
Previous versions | rss
Other tutorials and videos
Help video
Feedback

Protein

SRSF protein kinase 1

Gene

Srpk1

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Phosphorylates SFRS2, ZRSR2, LBR and PRM1. Phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Can induce splicing of exon 10 in MAPT/TAU (By similarity).By similarity2 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactorⁱ

Mg²⁺2 Publications

Activity regulationⁱ

Activated by phosphorylation on Ser-51 and Ser-548.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	109	ATPPROSITE-ProRule annotationBy similarity		1
Active siteⁱ	213	Proton acceptorPROSITE-ProRule annotationBy similarity		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	86 – 94	ATPPROSITE-ProRule annotationBy similarity		9

GO - Molecular functionⁱ

ATP binding
Source: UniProtKB
Inferred from direct assayⁱ
- 9446799
magnesium ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 9446799
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 9446799

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell differentiation Source: UniProtKB-KW
chromosome segregation Source: UniProtKB
intracellular signal transduction
Source: UniProtKB
Inferred from direct assayⁱ
- 9446799
negative regulation of viral genome replication Source: MGI
positive regulation of viral genome replication Source: MGI
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 9446799
regulation of mRNA processing
Source: UniProtKB
Inferred from direct assayⁱ
- 9446799
spliceosomal complex assembly Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Chromosome partition, Differentiation, mRNA processing, mRNA splicing
Ligand	ATP-binding, Nucleotide-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: SRSF protein kinase 1 (EC:2.7.11.1) Alternative name(s): SFRS protein kinase 1 Serine/arginine-rich protein-specific kinase 1 Short name: SR-protein-specific kinase 1
Gene namesⁱ	Name:Srpk1Imported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 17

Organism-specific databases

MGIⁱ	MGI:106908 Srpk1

Keywords - Cellular componentⁱ

Cytoplasm, Endoplasmic reticulum, Microsome, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000086675	1 – 648	SRSF protein kinase 1Add BLAST		648

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	51	PhosphoserineCombined sources	1
Modified residueⁱ	309	PhosphoserineCombined sources	1
Modified residueⁱ	311	PhosphoserineCombined sources	1
Modified residueⁱ	333	PhosphoserineBy similarity	1
Modified residueⁱ	448	PhosphothreonineCombined sources	1
Modified residueⁱ	450	PhosphoserineCombined sources	1
Modified residueⁱ	548	Phosphoserine; by CK2By similarity	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	O70551
PaxDbⁱ	O70551
PeptideAtlas More...PeptideAtlasⁱ	O70551
PRIDEⁱ	O70551

PTM databases

iPTMnetⁱ	O70551
PhosphoSitePlusⁱ	O70551

Expressionⁱ

Tissue specificityⁱ

Predominantly expressed in the testis but is also present at lower levels in heart, spleen, liver, brain, kidney, lung and skeletal muscle. Present in all germinal cells in the seminiferous tubules but not in mature spermatozoa.2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000004865 Expressed in 309 organ(s), highest expression level in ear
CleanExⁱ	MM_SRPK1
ExpressionAtlasⁱ	O70551 baseline and differential
Genevisibleⁱ	O70551 MM

Interactionⁱ

Subunit structureⁱ

Monomer. Found in a multisubunit complex containing seven proteins, named toposome, which separates entangled circular chromatin DNA during chromosome segregation. Interacts with HHV-1 ICP27 protein. Interacts with DNAJC8 and AHSA1/AHA1 and this mediates formation of a complex with the Hsp70 /Hsp90 machinery. Binds to IGF2BP1, SYNCRIP, HNRNPA2B1 and HNRNPC. Interacts with SAFB/SAFB1 and SAFB2 which inhibits its activity (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
SRSF1	Q07955	5	EBI-593343,EBI-398920	From Homo sapiens.

With

Entry

#Exp.

IntAct

Notes

SRSF1

Q07955

EBI-593343,EBI-398920

From Homo sapiens.

Protein-protein interaction databases

IntActⁱ	O70551, 3 interactors
Molecular INTeraction database More...MINTⁱ	O70551
STRINGⁱ	10090.ENSMUSP00000116259

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O70551
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	80 – 646	Protein kinasePROSITE-ProRule annotationAdd BLAST		567

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1290 Eukaryota ENOG410XRBH LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063566
HOGENOMⁱ	HOG000171558
HOVERGENⁱ	HBG108512
InParanoidⁱ	O70551
KEGG Orthology (KO) More...KOⁱ	K15409
OMAⁱ	EKYEWSK
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G05H9
PhylomeDBⁱ	O70551
TreeFamⁱ	TF105334

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069 Pkinase, 2 hits
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 2 hits
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

O70551-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MERKVLALQA RKKRTKAKKD KAQRKPETQH RGSAPHSESD IPEQEEEILG 
        60         70         80         90        100
SDDDEQEDPN DYCKGGYHLV KIGDLFNGRY HVIRKLGWGH FSTVWLSWDI 
       110        120        130        140        150
QGKKFVAMKV VKSAEHYTET ALDEIRLLKS VRNSDPNDPN GEMVVQLLDD 
       160        170        180        190        200
FKISGVNGTH ICMVFEVLGH HLLKWIIKSN YQGLPLPCVK KIIQQVLQGL 
       210        220        230        240        250
DYLHTKCRII HTDIKPENIL LSVNEQYIRR LAAEATEWQR SGAPPPSGSA 
       260        270        280        290        300
VSTAPQPKPA DKMSKNKKKK LKKKQKRQAE LLEKRMQEIE EMEKESGPGQ 
       310        320        330        340        350
KRPNKQEESE SPVDRPLTEN PPNKMTQEKL EESNSIGQDQ TLTERGGEGG 
       360        370        380        390        400
APEINCNGVI GVVNYPENSN NETLRHKEDL HNANDCDVHT LKQEPSFLNS 
       410        420        430        440        450
SNGDSSPSQD TDSCTPTASE TMVCQSSAEQ SLTRQDITQL EESIRADTPS 
       460        470        480        490        500
GDEQEPNGAL DSKGKFSAGN FLINPLEPKN AEKLQVKIAD LGNACWVHKH 
       510        520        530        540        550
FTEDIQTRQY RSLEVLIGSG YNTPADIWST ACMAFELATG DYLFEPHSGE 
       560        570        580        590        600
DYTRDEDHIA LIIELLGKVP RKLIVAGKYS KEFFTKKGDL KHITKLKPWG 
       610        620        630        640 
LLEVLVEKYE WPQEEAAGFT DFLLPMLELM PEKRATAAEC LRHPWLNS

Length:648

Mass (Da):73,088

Last modified:June 21, 2005 - v2

Checksum:ⁱCB779C163A11ECEB

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F8WGJ7	F8WGJ7_MOUSE	SRSF protein kinase 1 SRSF protein kinase 1	Srpk1	215	Annotation score:
B2KF32	B2KF32_MOUSE	SRSF protein kinase 1 SRSF protein kinase 1	Srpk1	35	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	113	S → I in CAA11833 (PubMed:10390541).Curated	1
Sequence conflictⁱ	155	G → V in CAA11833 (PubMed:10390541).Curated	1
Sequence conflictⁱ	256	Q → T in CAA11833 (PubMed:10390541).Curated	1
Sequence conflictⁱ	279	A → P in BAA25299 (PubMed:9446799).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB012290 mRNA Translation: BAA25299.1 AJ224115 mRNA Translation: CAA11833.1 BC005707 mRNA Translation: AAH05707.1 BC050761 mRNA Translation: AAH50761.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS37531.1
PIRⁱ	JC5930
NCBI Reference Sequences More...RefSeqⁱ	NP_058075.2, NM_016795.4
UniGeneⁱ	Mm.15252 Mm.479188

Genome annotation databases

Ensemblⁱ	ENSMUST00000130643; ENSMUSP00000116259; ENSMUSG00000004865
GeneIDⁱ	20815
KEGGⁱ	mmu:20815
UCSC genome browser More...UCSCⁱ	uc008bri.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O70551	Uncharacterized protein		MOUSE		648	UniRef100_O70551
Uncharacterized protein (Fragment)		MOUSE		465

Protein	Similar proteins		Species	Score	Length	Source
O70551	SRSF protein kinase 1	)	HUMAN		655	UniRef90_Q96SB4
SRSF protein kinase 1		PONAB		655
SRSF protein kinase 1		GORGO		655
Uncharacterized protein		PIG		766
Uncharacterized protein		MACNE		700
+108

Protein	Similar proteins		Species	Score	Length	Source
O70551	SRSF protein kinase 1	)	HUMAN		655	UniRef50_Q96SB4
SRSF protein kinase 1		PONAB		655
SRSF protein kinase 1		GORGO		655
Uncharacterized protein		PIG		766
Uncharacterized protein		MACNE		700
+171

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB012290 mRNA Translation: BAA25299.1 AJ224115 mRNA Translation: CAA11833.1 BC005707 mRNA Translation: AAH05707.1 BC050761 mRNA Translation: AAH50761.1
CCDSⁱ	CCDS37531.1
PIRⁱ	JC5930
RefSeqⁱ	NP_058075.2, NM_016795.4
UniGeneⁱ	Mm.15252 Mm.479188

3D structure databases

ProteinModelPortalⁱ	O70551
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	O70551, 3 interactors
MINTⁱ	O70551
STRINGⁱ	10090.ENSMUSP00000116259

PTM databases

iPTMnetⁱ	O70551
PhosphoSitePlusⁱ	O70551

Proteomic databases

EPDⁱ	O70551
PaxDbⁱ	O70551
PeptideAtlasⁱ	O70551
PRIDEⁱ	O70551

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000130643; ENSMUSP00000116259; ENSMUSG00000004865
GeneIDⁱ	20815
KEGGⁱ	mmu:20815
UCSCⁱ	uc008bri.1 mouse

Organism-specific databases

CTDⁱ	6732
MGIⁱ	MGI:106908 Srpk1

Phylogenomic databases

eggNOGⁱ	KOG1290 Eukaryota ENOG410XRBH LUCA
GeneTreeⁱ	ENSGT00530000063566
HOGENOMⁱ	HOG000171558
HOVERGENⁱ	HBG108512
InParanoidⁱ	O70551
KOⁱ	K15409
OMAⁱ	EKYEWSK
OrthoDBⁱ	EOG091G05H9
PhylomeDBⁱ	O70551
TreeFamⁱ	TF105334

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O70551
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000004865 Expressed in 309 organ(s), highest expression level in ear
CleanExⁱ	MM_SRPK1
ExpressionAtlasⁱ	O70551 baseline and differential
Genevisibleⁱ	O70551 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS
Pfamⁱ	View protein in Pfam PF00069 Pkinase, 2 hits
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit
SUPFAMⁱ	SSF56112 SSF56112, 2 hits
PROSITEⁱ	View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SRPK1_MOUSE
Accessionⁱ	O70551Primary (citable) accession number: O70551 Secondary accession number(s): O70193, Q99JT3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
	Last sequence update:	June 21, 2005
	Last modified:	September 12, 2018
	This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

Human and mouse protein kinases
Human and mouse protein kinases: classification and index
SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O70551 (SRPK1_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

SRSF protein kinase 1

Srpk1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Endoplasmic reticulum

Other locations

Cytosol

Endoplasmic reticulum

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ