UniProtKB - O70551 (SRPK1_MOUSE)
SRSF protein kinase 1
Srpk1
Functioni
Catalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 109 | ATPPROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 213 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 86 – 94 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- MAP kinase activity Source: GO_Central
- protein serine/threonine kinase activity Source: UniProtKB
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB
- intracellular signal transduction Source: UniProtKB
- negative regulation of viral genome replication Source: MGI
- positive regulation of viral genome replication Source: MGI
- protein phosphorylation Source: UniProtKB
- regulation of gene expression Source: GO_Central
- regulation of mRNA processing Source: UniProtKB
- spliceosomal complex assembly Source: GO_Central
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Chromosome partition, Differentiation, mRNA processing, mRNA splicing |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: SRSF protein kinase 1 (EC:2.7.11.1)Alternative name(s): SFRS protein kinase 1 Serine/arginine-rich protein-specific kinase 1 Short name: SR-protein-specific kinase 1 |
Gene namesi | Name:Srpk1Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106908, Srpk1 |
Subcellular locationi
Endoplasmic reticulum
- Microsome By similarity
Nucleus
- nucleoplasm By similarity
- Nucleus matrix By similarity
- Nucleus speckle By similarity
Other locations
- Cytoplasm By similarity
- Chromosome By similarity
Note: Shuttles between the nucleus and the cytoplasm (By similarity). Inhibition of the Hsp90 ATPase activity, osmotic stress and interaction with HHV-1 ICP27 protein can induce its translocation to the nucleus (By similarity). KAT5/TIP60 inhibits its nuclear translocation (By similarity). Preferentially localizes to the promoter of gene coding regions (By similarity).By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-KW
Nucleus
- nuclear matrix Source: UniProtKB-SubCell
- nuclear speck Source: UniProtKB
- nucleoplasm Source: UniProtKB
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
Keywords - Cellular componenti
Chromosome, Cytoplasm, Endoplasmic reticulum, Microsome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086675 | 1 – 648 | SRSF protein kinase 1Add BLAST | 648 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 51 | PhosphoserineCombined sources | 1 | |
Modified residuei | 309 | PhosphoserineCombined sources | 1 | |
Modified residuei | 311 | PhosphoserineCombined sources | 1 | |
Modified residuei | 333 | PhosphoserineBy similarity | 1 | |
Modified residuei | 448 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 450 | PhosphoserineCombined sources | 1 | |
Modified residuei | 548 | Phosphoserine; by CK2By similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O70551 |
jPOSTi | O70551 |
PaxDbi | O70551 |
PeptideAtlasi | O70551 |
PRIDEi | O70551 |
PTM databases
iPTMneti | O70551 |
PhosphoSitePlusi | O70551 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000004865, Expressed in secondary oocyte and 324 other tissues |
ExpressionAtlasi | O70551, baseline and differential |
Genevisiblei | O70551, MM |
Interactioni
Subunit structurei
Monomer.
Found in a multisubunit complex containing seven proteins, named toposome, which separates entangled circular chromatin DNA during chromosome segregation.
Interacts with HHV-1 ICP27 protein.
Interacts with DNAJC8 and AHSA1/AHA1 and this mediates formation of a complex with the Hsp70 /Hsp90 machinery. Binds to IGF2BP1, SYNCRIP, HNRNPA2B1 and HNRNPC.
Interacts with SAFB/SAFB1 and SAFB2 which inhibits its activity (By similarity).
By similarityBinary interactionsi
O70551
With | #Exp. | IntAct |
---|---|---|
SRSF1 [Q07955] from Homo sapiens. | 5 | EBI-593343,EBI-398920 |
Protein-protein interaction databases
BioGRIDi | 203502, 1 interactor |
IntActi | O70551, 3 interactors |
MINTi | O70551 |
STRINGi | 10090.ENSMUSP00000116259 |
Chemistry databases
BindingDBi | O70551 |
Miscellaneous databases
RNActi | O70551, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 80 – 646 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 567 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1290, Eukaryota |
GeneTreei | ENSGT00940000155264 |
HOGENOMi | CLU_000288_81_9_1 |
InParanoidi | O70551 |
OMAi | HEGQRPP |
OrthoDBi | 290680at2759 |
PhylomeDBi | O70551 |
TreeFami | TF105334 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MERKVLALQA RKKRTKAKKD KAQRKPETQH RGSAPHSESD IPEQEEEILG
60 70 80 90 100
SDDDEQEDPN DYCKGGYHLV KIGDLFNGRY HVIRKLGWGH FSTVWLSWDI
110 120 130 140 150
QGKKFVAMKV VKSAEHYTET ALDEIRLLKS VRNSDPNDPN GEMVVQLLDD
160 170 180 190 200
FKISGVNGTH ICMVFEVLGH HLLKWIIKSN YQGLPLPCVK KIIQQVLQGL
210 220 230 240 250
DYLHTKCRII HTDIKPENIL LSVNEQYIRR LAAEATEWQR SGAPPPSGSA
260 270 280 290 300
VSTAPQPKPA DKMSKNKKKK LKKKQKRQAE LLEKRMQEIE EMEKESGPGQ
310 320 330 340 350
KRPNKQEESE SPVDRPLTEN PPNKMTQEKL EESNSIGQDQ TLTERGGEGG
360 370 380 390 400
APEINCNGVI GVVNYPENSN NETLRHKEDL HNANDCDVHT LKQEPSFLNS
410 420 430 440 450
SNGDSSPSQD TDSCTPTASE TMVCQSSAEQ SLTRQDITQL EESIRADTPS
460 470 480 490 500
GDEQEPNGAL DSKGKFSAGN FLINPLEPKN AEKLQVKIAD LGNACWVHKH
510 520 530 540 550
FTEDIQTRQY RSLEVLIGSG YNTPADIWST ACMAFELATG DYLFEPHSGE
560 570 580 590 600
DYTRDEDHIA LIIELLGKVP RKLIVAGKYS KEFFTKKGDL KHITKLKPWG
610 620 630 640
LLEVLVEKYE WPQEEAAGFT DFLLPMLELM PEKRATAAEC LRHPWLNS
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WGJ7 | F8WGJ7_MOUSE | SRSF protein kinase 1 | Srpk1 | 223 | Annotation score: | ||
A0A3B2W430 | A0A3B2W430_MOUSE | SRSF protein kinase 1 | Srpk1 | 280 | Annotation score: | ||
A0A3B2W4B9 | A0A3B2W4B9_MOUSE | SRSF protein kinase 1 | Srpk1 | 307 | Annotation score: | ||
A0A3B2W883 | A0A3B2W883_MOUSE | SRSF protein kinase 1 | Srpk1 | 345 | Annotation score: | ||
A0A3B2W7I6 | A0A3B2W7I6_MOUSE | SRSF protein kinase 1 | Srpk1 | 146 | Annotation score: | ||
B2KF32 | B2KF32_MOUSE | SRSF protein kinase 1 | Srpk1 | 35 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 113 | S → I in CAA11833 (PubMed:10390541).Curated | 1 | |
Sequence conflicti | 155 | G → V in CAA11833 (PubMed:10390541).Curated | 1 | |
Sequence conflicti | 256 | Q → T in CAA11833 (PubMed:10390541).Curated | 1 | |
Sequence conflicti | 279 | A → P in BAA25299 (PubMed:9446799).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB012290 mRNA Translation: BAA25299.1 AJ224115 mRNA Translation: CAA11833.1 BC005707 mRNA Translation: AAH05707.1 BC050761 mRNA Translation: AAH50761.1 |
CCDSi | CCDS37531.1 |
PIRi | JC5930 |
RefSeqi | NP_058075.2, NM_016795.4 |
Genome annotation databases
Ensembli | ENSMUST00000130643; ENSMUSP00000116259; ENSMUSG00000004865 |
GeneIDi | 20815 |
KEGGi | mmu:20815 |
UCSCi | uc008bri.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB012290 mRNA Translation: BAA25299.1 AJ224115 mRNA Translation: CAA11833.1 BC005707 mRNA Translation: AAH05707.1 BC050761 mRNA Translation: AAH50761.1 |
CCDSi | CCDS37531.1 |
PIRi | JC5930 |
RefSeqi | NP_058075.2, NM_016795.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 203502, 1 interactor |
IntActi | O70551, 3 interactors |
MINTi | O70551 |
STRINGi | 10090.ENSMUSP00000116259 |
Chemistry databases
BindingDBi | O70551 |
ChEMBLi | CHEMBL4105750 |
PTM databases
iPTMneti | O70551 |
PhosphoSitePlusi | O70551 |
Proteomic databases
EPDi | O70551 |
jPOSTi | O70551 |
PaxDbi | O70551 |
PeptideAtlasi | O70551 |
PRIDEi | O70551 |
Protocols and materials databases
Antibodypediai | 6714, 443 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000130643; ENSMUSP00000116259; ENSMUSG00000004865 |
GeneIDi | 20815 |
KEGGi | mmu:20815 |
UCSCi | uc008bri.1, mouse |
Organism-specific databases
CTDi | 6732 |
MGIi | MGI:106908, Srpk1 |
Phylogenomic databases
eggNOGi | KOG1290, Eukaryota |
GeneTreei | ENSGT00940000155264 |
HOGENOMi | CLU_000288_81_9_1 |
InParanoidi | O70551 |
OMAi | HEGQRPP |
OrthoDBi | 290680at2759 |
PhylomeDBi | O70551 |
TreeFami | TF105334 |
Miscellaneous databases
BioGRID-ORCSi | 20815, 3 hits in 19 CRISPR screens |
ChiTaRSi | Srpk1, mouse |
PROi | PR:O70551 |
RNActi | O70551, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004865, Expressed in secondary oocyte and 324 other tissues |
ExpressionAtlasi | O70551, baseline and differential |
Genevisiblei | O70551, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 2 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SRPK1_MOUSE | |
Accessioni | O70551Primary (citable) accession number: O70551 Secondary accession number(s): O70193, Q99JT3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 21, 2005 |
Last sequence update: | June 21, 2005 | |
Last modified: | December 2, 2020 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index