UniProtKB - O70546 (KDM6A_MOUSE)
Lysine-specific demethylase 6A
Kdm6a
Functioni
Miscellaneous
Catalytic activityi
- 2 2-oxoglutarate + N6,N6,N6-trimethyl-L-lysyl27-[histone H3] + 2 O2 = 2 CO2 + 2 formaldehyde + N6-methyl-L-lysyl27-[histone H3] + 2 succinateBy similarityEC:1.14.11.68By similarity
Cofactori
Protein has several cofactor binding sites:- L-ascorbateBy similarity
- Fe2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 1146 | IronBy similarity | 1 | |
Metal bindingi | 1148 | IronBy similarity | 1 | |
Metal bindingi | 1226 | IronBy similarity | 1 | |
Metal bindingi | 1331 | ZincBy similarity | 1 | |
Metal bindingi | 1334 | ZincBy similarity | 1 | |
Metal bindingi | 1358 | ZincBy similarity | 1 | |
Metal bindingi | 1361 | ZincBy similarity | 1 |
GO - Molecular functioni
- chromatin binding Source: MGI
- chromatin DNA binding Source: MGI
- dioxygenase activity Source: UniProtKB-KW
- histone demethylase activity (H3-K27 specific) Source: MGI
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- canonical Wnt signaling pathway Source: MGI
- chromatin remodeling Source: UniProtKB
- circulatory system development Source: MGI
- embryonic organ development Source: MGI
- heart development Source: MGI
- heart morphogenesis Source: MGI
- histone H3-K27 demethylation Source: MGI
- in utero embryonic development Source: MGI
- mesodermal cell differentiation Source: MGI
- multicellular organism growth Source: MGI
- neural tube closure Source: MGI
- neural tube development Source: MGI
- notochord morphogenesis Source: MGI
- positive regulation of gene expression Source: MGI
- regulation of gene expression Source: MGI
- respiratory system process Source: MGI
- somite rostral/caudal axis specification Source: MGI
Keywordsi
Molecular function | Chromatin regulator, Dioxygenase, Oxidoreductase |
Ligand | Iron, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-3214842, HDMs demethylate histones |
Names & Taxonomyi
Protein namesi | Recommended name: Lysine-specific demethylase 6A (EC:1.14.11.68By similarity)Alternative name(s): Histone demethylase UTX Ubiquitously transcribed TPR protein on the X chromosome Ubiquitously transcribed X chromosome tetratricopeptide repeat protein [histone H3]-trimethyl-L-lysine(27) demethylase 6ACurated |
Gene namesi | Name:Kdm6a Synonyms:Utx |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1095419, Kdm6a |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- histone methyltransferase complex Source: MGI
- MLL3/4 complex Source: MGI
- nuclear chromatin Source: GO_Central
- nucleoplasm Source: Reactome
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106410 | 1 – 1401 | Lysine-specific demethylase 6AAdd BLAST | 1401 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 519 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 549 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 769 | PhosphoserineCombined sources | 1 | |
Modified residuei | 827 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 829 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | O70546 |
MaxQBi | O70546 |
PaxDbi | O70546 |
PeptideAtlasi | O70546 |
PRIDEi | O70546 |
PTM databases
iPTMneti | O70546 |
PhosphoSitePlusi | O70546 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000037369, Expressed in secondary oocyte and 297 other tissues |
ExpressionAtlasi | O70546, baseline and differential |
Genevisiblei | O70546, MM |
Interactioni
Subunit structurei
Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A (or KDM6B), PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin (By similarity).
Interacts with TLE1 (PubMed:9854018).
Interacts with SUPT6H (PubMed:23503590).
Interacts with SMARCA4 (PubMed:21095589).
By similarity3 PublicationsBinary interactionsi
O70546
With | #Exp. | IntAct |
---|---|---|
Six4 [Q61321] | 2 | EBI-1573712,EBI-986524 |
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204471, 8 interactors |
IntActi | O70546, 6 interactors |
MINTi | O70546 |
STRINGi | 10090.ENSMUSP00000061539 |
Miscellaneous databases
RNActi | O70546, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 95 – 128 | TPR 1Add BLAST | 34 | |
Repeati | 132 – 165 | TPR 2Add BLAST | 34 | |
Repeati | 169 – 203 | TPR 3Add BLAST | 35 | |
Repeati | 207 – 240 | TPR 4Add BLAST | 34 | |
Repeati | 245 – 285 | TPR 5Add BLAST | 41 | |
Repeati | 286 – 319 | TPR 6Add BLAST | 34 | |
Repeati | 321 – 353 | TPR 7Add BLAST | 33 | |
Repeati | 355 – 387 | TPR 8Add BLAST | 33 | |
Domaini | 1095 – 1258 | JmjCPROSITE-ProRule annotationAdd BLAST | 164 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 1095 | Interaction with SUPT6H1 PublicationAdd BLAST | 1095 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 9 – 19 | Poly-AlaAdd BLAST | 11 | |
Compositional biasi | 919 – 941 | Pro-richAdd BLAST | 23 |
Sequence similaritiesi
Keywords - Domaini
Repeat, TPR repeatPhylogenomic databases
eggNOGi | KOG1124, Eukaryota KOG1246, Eukaryota |
GeneTreei | ENSGT00940000155202 |
HOGENOMi | CLU_003187_0_0_1 |
InParanoidi | O70546 |
OMAi | LCESQPE |
OrthoDBi | 268901at2759 |
TreeFami | TF317405 |
Family and domain databases
Gene3Di | 1.25.40.10, 2 hits |
InterProi | View protein in InterPro IPR003347, JmjC_dom IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF02373, JmjC, 1 hit PF13181, TPR_8, 1 hit |
SMARTi | View protein in SMART SM00558, JmjC, 1 hit SM00028, TPR, 6 hits |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS51184, JMJC, 1 hit PS50005, TPR, 7 hits PS50293, TPR_REGION, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKSCGVSLAT AAAAAAAAAF GDEEKKMAAG KASGESEEAS PSLTAEEREA
60 70 80 90 100
LGGLDSRLFG FVRFHEDGAR MKALLGKAVR CYESLILKAE GKVESDFFCQ
110 120 130 140 150
LGHFNLLLED YPKALSAYQR YYSLQSDYWK NAAFLYGLGL VYFHYNAFQW
160 170 180 190 200
AIKAFQEVLY VDPSFCRAKE IHLRLGLMFK VNTDYESSLK HFQLALVDCN
210 220 230 240 250
PCTLSNAEIQ FHIAHLYETQ RKYHSAKEAY EQLLQTENLS AQVKATILQQ
260 270 280 290 300
LGWMHHTVDL LGDKATKESY AIQYLQKSLE ADPNSGQSWY FLGRCYSSIG
310 320 330 340 350
KVQDAFISYR QSIDKSEASA DTWCSIGVLY QQQNQPMDAL QAYICAVQLD
360 370 380 390 400
HGHAAAWMDL GTLYESCNQP QDAIKCYLNA TRSKNCSNTS GLAARIKYLQ
410 420 430 440 450
AQLCNLPQGS LQNKTKLLPS IEEAWSLPIP AELTSRQGAM NTAQQNTSDN
460 470 480 490 500
WSGGNAPPPV EQQTHSWCLT PQKLQHLEQL RANRNNLNPA QKLMLEQLES
510 520 530 540 550
QFVLMQQHQM RQTGVAQVRP TGILNGPTVD SSLPTNSVSG QQPQLPLTRM
560 570 580 590 600
PSVSQPGVHT ACPRQTLANG PFSAGHVPCS TSRTLGSTDT VLIGNNHVTG
610 620 630 640 650
SGSNGNVPYL QRNAPTLPHN RTNLTSSTEE PWKNQLSNST QGLHKGPSSH
660 670 680 690 700
LAGPNGERPL SSTGPSQHLQ AAGSGIQNQN GHPTLPSNSV TQGAALNHLS
710 720 730 740 750
SHTATSGGQQ GITLTKESKP SGNTLTVPET SRQTGETPNS TASVEGLPNH
760 770 780 790 800
VHQVMADAVC SPSHGDSKSP GLLSSDNPQL SALLMGKANN NVGPGTCDKV
810 820 830 840 850
NNIHPTVHTK TDNSVASSPS SAISTATPSP KSTEQTTTNS VTSLNSPHSG
860 870 880 890 900
LHTINGEGME ESQSPIKTDL LLVSHRPSPQ IIPSMSVSIY PSSAEVLKAC
910 920 930 940 950
RNLGKNGLSN SSILLDKCPP PRPPSSPYPP LPKDKLNPPT PSIYLENKRD
960 970 980 990 1000
AFFPPLHQFC TNPNNPVTVI RGLAGALKLD LGLFSTKTLV EANNEHMVEV
1010 1020 1030 1040 1050
RTQLLQPADE NWDPTGTKKI WHCESNRSHT TIAKYAQYQA SSFQESLREE
1060 1070 1080 1090 1100
NEKRSHHKDH SDSESTSSDN SGKRRKGPFK TIKFGTNIDL SDDKKWKLQL
1110 1120 1130 1140 1150
HELTKLPAFV RVVSAGNLLS HVGHTILGMN TVQLYMKVPG SRTPGHQENN
1160 1170 1180 1190 1200
NFCSVNINIG PGDCEWFVVP EGYWGVLNDF CEKNNLNFLM GSWWPNLEDL
1210 1220 1230 1240 1250
YEANVPVYRF IQRPGDLVWI NAGTVHWVQA IGWCNNIAWN VGPLTACQYK
1260 1270 1280 1290 1300
LAVERYEWNK LQNVKSIVPM VHLSWNMARN IKVSDPKLFE MIKYCLLRTL
1310 1320 1330 1340 1350
KQCQTLREAL IAAGKEIIWH GRTKEEPAHY CSICEVEVFD LLFVTNESNS
1360 1370 1380 1390 1400
RKTYIVHCQD CARKTSGNLE NFVVLEQYKM EDLMQVYDQF TLAPPLPSAS
S
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A286YCW2 | A0A286YCW2_MOUSE | Lysine-specific demethylase 6A | Kdm6a | 236 | Annotation score: | ||
A0A286YD05 | A0A286YD05_MOUSE | Lysine-specific demethylase 6A | Kdm6a | 997 | Annotation score: | ||
A0A286YD51 | A0A286YD51_MOUSE | Lysine-specific demethylase 6A | Kdm6a | 369 | Annotation score: | ||
A0A286YDX1 | A0A286YDX1_MOUSE | Lysine-specific demethylase 6A | Kdm6a | 224 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 321 | D → G in AAH75703 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1198 | E → G in AAH75703 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_022196 | 1393 – 1401 | APPLPSASS → VSEINMLLHYHPPHLDIVPW TLNMRPFLLFRK in isoform 2. 1 Publication | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL732451 Genomic DNA No translation available. AL773547 Genomic DNA No translation available. BC053433 mRNA Translation: AAH53433.1 BC075703 mRNA Translation: AAH75703.1 Different initiation. AJ002730 mRNA Translation: CAA05692.1 |
CCDSi | CCDS30037.1 [O70546-2] CCDS81102.1 [O70546-1] |
RefSeqi | NP_001297373.1, NM_001310444.1 [O70546-1] NP_033509.1, NM_009483.2 [O70546-2] |
Genome annotation databases
Ensembli | ENSMUST00000044484; ENSMUSP00000045862; ENSMUSG00000037369 [O70546-1] ENSMUST00000052368; ENSMUSP00000061539; ENSMUSG00000037369 [O70546-2] |
GeneIDi | 22289 |
KEGGi | mmu:22289 |
UCSCi | uc009ssk.1, mouse [O70546-1] uc009ssm.1, mouse [O70546-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL732451 Genomic DNA No translation available. AL773547 Genomic DNA No translation available. BC053433 mRNA Translation: AAH53433.1 BC075703 mRNA Translation: AAH75703.1 Different initiation. AJ002730 mRNA Translation: CAA05692.1 |
CCDSi | CCDS30037.1 [O70546-2] CCDS81102.1 [O70546-1] |
RefSeqi | NP_001297373.1, NM_001310444.1 [O70546-1] NP_033509.1, NM_009483.2 [O70546-2] |
3D structure databases
SMRi | O70546 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204471, 8 interactors |
IntActi | O70546, 6 interactors |
MINTi | O70546 |
STRINGi | 10090.ENSMUSP00000061539 |
PTM databases
iPTMneti | O70546 |
PhosphoSitePlusi | O70546 |
Proteomic databases
EPDi | O70546 |
MaxQBi | O70546 |
PaxDbi | O70546 |
PeptideAtlasi | O70546 |
PRIDEi | O70546 |
Protocols and materials databases
Antibodypediai | 639, 193 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000044484; ENSMUSP00000045862; ENSMUSG00000037369 [O70546-1] ENSMUST00000052368; ENSMUSP00000061539; ENSMUSG00000037369 [O70546-2] |
GeneIDi | 22289 |
KEGGi | mmu:22289 |
UCSCi | uc009ssk.1, mouse [O70546-1] uc009ssm.1, mouse [O70546-2] |
Organism-specific databases
CTDi | 7403 |
MGIi | MGI:1095419, Kdm6a |
Phylogenomic databases
eggNOGi | KOG1124, Eukaryota KOG1246, Eukaryota |
GeneTreei | ENSGT00940000155202 |
HOGENOMi | CLU_003187_0_0_1 |
InParanoidi | O70546 |
OMAi | LCESQPE |
OrthoDBi | 268901at2759 |
TreeFami | TF317405 |
Enzyme and pathway databases
Reactomei | R-MMU-3214842, HDMs demethylate histones |
Miscellaneous databases
BioGRID-ORCSi | 22289, 0 hits in 14 CRISPR screens |
ChiTaRSi | Kdm6a, mouse |
PROi | PR:O70546 |
RNActi | O70546, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037369, Expressed in secondary oocyte and 297 other tissues |
ExpressionAtlasi | O70546, baseline and differential |
Genevisiblei | O70546, MM |
Family and domain databases
Gene3Di | 1.25.40.10, 2 hits |
InterProi | View protein in InterPro IPR003347, JmjC_dom IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF02373, JmjC, 1 hit PF13181, TPR_8, 1 hit |
SMARTi | View protein in SMART SM00558, JmjC, 1 hit SM00028, TPR, 6 hits |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS51184, JMJC, 1 hit PS50005, TPR, 7 hits PS50293, TPR_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KDM6A_MOUSE | |
Accessioni | O70546Primary (citable) accession number: O70546 Secondary accession number(s): A2AID2, Q6DI80, Q7TSG4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | January 9, 2007 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families