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Protein

Very-long-chain 3-oxoacyl-CoA reductase

Gene

Hsd17b12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.By similarity

Catalytic activityi

A very-long-chain (3R)-3-hydroxyacyl-CoA + NADP+ = a very-long-chain 3-oxoacyl-CoA + NADPH.By similarity
17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.By similarity

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Pathwayi: estrogen biosynthesis

This protein is involved in the pathway estrogen biosynthesis, which is part of Steroid biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway estrogen biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189SubstrateBy similarity1
Active sitei202Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi50 – 79NADPBy similarityAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis
LigandNADP

Enzyme and pathway databases

ReactomeiR-MMU-193048 Androgen biosynthesis
R-MMU-75876 Synthesis of very long-chain fatty acyl-CoAs
UniPathwayi
UPA00094

UPA00769

Chemistry databases

SwissLipidsiSLP:000000434

Names & Taxonomyi

Protein namesi
Recommended name:
Very-long-chain 3-oxoacyl-CoA reductaseCurated (EC:1.1.1.330By similarity)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 12By similarity
Short name:
17-beta-HSD 12By similarity
3-ketoacyl-CoA reductaseBy similarity
Short name:
KARBy similarity
Estradiol 17-beta-dehydrogenase 12By similarity (EC:1.1.1.62By similarity)
KIK-I
Gene namesi
Name:Hsd17b12Imported
Synonyms:Kik1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1926967 Hsd17b12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Transmembranei269 – 285HelicalSequence analysisAdd BLAST17

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000545761 – 312Very-long-chain 3-oxoacyl-CoA reductaseAdd BLAST312

Proteomic databases

EPDiO70503
PaxDbiO70503
PeptideAtlasiO70503
PRIDEiO70503

PTM databases

iPTMnetiO70503
PhosphoSitePlusiO70503
SwissPalmiO70503

Expressioni

Tissue specificityi

Expressed in most tissues tested.1 Publication

Gene expression databases

BgeeiENSMUSG00000027195 Expressed in 304 organ(s), highest expression level in cochlea
CleanExiMM_HSD17B12
ExpressionAtlasiO70503 baseline and differential
GenevisibleiO70503 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207914, 2 interactors
IntActiO70503, 6 interactors
MINTiO70503
STRINGi10090.ENSMUSP00000028619

Structurei

3D structure databases

ProteinModelPortaliO70503
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi308 – 312Di-lysine motifBy similarity5

Domaini

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1014 Eukaryota
COG0300 LUCA
GeneTreeiENSGT00390000010069
HOGENOMiHOG000039237
HOVERGENiHBG005478
InParanoidiO70503
KOiK10251
OMAiYLVTSAM
OrthoDBiEOG091G06T2
PhylomeDBiO70503
TreeFamiTF314591

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O70503-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MECAPPAAGF LYWVGASTIA YLALRASYSL FRAFQVWCVG NEALVGPRLG
60 70 80 90 100
EWAVVTGGTD GIGKAYAEEL AKRGMKIVLI SRSQDKLNQV SNNIKEKFNV
110 120 130 140 150
ETRTIAVDFS LDDIYDKIKT GLSGLEIGVL VNNVGMSYEY PEYFLEIPDL
160 170 180 190 200
DNTIKKLINI NVLSVCKVTR LVLPGMVERS KGVILNISSA SGMLPVPLLT
210 220 230 240 250
IYSATKAFVD FFSQCLHEEY KSKGIFVQSV MPYLVATKLA KIQKPTLDKP
260 270 280 290 300
SAETFVKSAI KTVGLQTRTT GYVIHSLMGS INSIMPRWMY FKIIMGFSKS
310
LRNRYLKKRK KN
Length:312
Mass (Da):34,742
Last modified:August 1, 1998 - v1
Checksum:i7D255188FA1F8DDB
GO
Isoform 2 (identifier: O70503-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-135: Missing.

Note: No experimental confirmation available.
Show »
Length:177
Mass (Da):20,127
Checksum:iCAC34046DDAE3A0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2E → V in BAE39915 (PubMed:16141072).Curated1
Sequence conflicti256V → M in BAE29906 (PubMed:16141072).Curated1
Sequence conflicti256V → M in BAE31260 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0202551 – 135Missing in isoform 2. 1 PublicationAdd BLAST135

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064635 mRNA Translation: AAC16885.1
AK078841 mRNA Translation: BAC37418.1
AK081869 mRNA Translation: BAC38354.1
AK082715 mRNA Translation: BAC38583.1
AK088521 mRNA Translation: BAC40401.1
AK150849 mRNA Translation: BAE29906.1
AK152489 mRNA Translation: BAE31260.1
AK166027 mRNA Translation: BAE38530.1
AK167908 mRNA Translation: BAE39915.1
BC037620 mRNA Translation: AAH37620.1
CCDSiCCDS16458.1 [O70503-1]
RefSeqiNP_062631.1, NM_019657.4 [O70503-1]
UniGeneiMm.22505

Genome annotation databases

EnsembliENSMUST00000028619; ENSMUSP00000028619; ENSMUSG00000027195 [O70503-1]
GeneIDi56348
KEGGimmu:56348
UCSCiuc008lgp.1 mouse [O70503-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF064635 mRNA Translation: AAC16885.1
AK078841 mRNA Translation: BAC37418.1
AK081869 mRNA Translation: BAC38354.1
AK082715 mRNA Translation: BAC38583.1
AK088521 mRNA Translation: BAC40401.1
AK150849 mRNA Translation: BAE29906.1
AK152489 mRNA Translation: BAE31260.1
AK166027 mRNA Translation: BAE38530.1
AK167908 mRNA Translation: BAE39915.1
BC037620 mRNA Translation: AAH37620.1
CCDSiCCDS16458.1 [O70503-1]
RefSeqiNP_062631.1, NM_019657.4 [O70503-1]
UniGeneiMm.22505

3D structure databases

ProteinModelPortaliO70503
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207914, 2 interactors
IntActiO70503, 6 interactors
MINTiO70503
STRINGi10090.ENSMUSP00000028619

Chemistry databases

SwissLipidsiSLP:000000434

PTM databases

iPTMnetiO70503
PhosphoSitePlusiO70503
SwissPalmiO70503

Proteomic databases

EPDiO70503
PaxDbiO70503
PeptideAtlasiO70503
PRIDEiO70503

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028619; ENSMUSP00000028619; ENSMUSG00000027195 [O70503-1]
GeneIDi56348
KEGGimmu:56348
UCSCiuc008lgp.1 mouse [O70503-1]

Organism-specific databases

CTDi51144
MGIiMGI:1926967 Hsd17b12

Phylogenomic databases

eggNOGiKOG1014 Eukaryota
COG0300 LUCA
GeneTreeiENSGT00390000010069
HOGENOMiHOG000039237
HOVERGENiHBG005478
InParanoidiO70503
KOiK10251
OMAiYLVTSAM
OrthoDBiEOG091G06T2
PhylomeDBiO70503
TreeFamiTF314591

Enzyme and pathway databases

UniPathwayi
UPA00094

UPA00769

ReactomeiR-MMU-193048 Androgen biosynthesis
R-MMU-75876 Synthesis of very long-chain fatty acyl-CoAs

Miscellaneous databases

ChiTaRSiHsd17b12 mouse
PROiPR:O70503
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027195 Expressed in 304 organ(s), highest expression level in cochlea
CleanExiMM_HSD17B12
ExpressionAtlasiO70503 baseline and differential
GenevisibleiO70503 MM

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDHB12_MOUSE
AccessioniPrimary (citable) accession number: O70503
Secondary accession number(s): Q3TID1
, Q3U7V9, Q542N3, Q8CI39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: October 10, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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