UniProtKB - O70494 (SP3_MOUSE)
Transcription factor Sp3
Sp3
Functioni
Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping (By similarity).
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 623 – 647 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 653 – 677 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 683 – 705 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- chromatin binding Source: MGI
- cis-regulatory region sequence-specific DNA binding Source: MGI
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: UniProtKB
- double-stranded DNA binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- B cell differentiation Source: MGI
- definitive hemopoiesis Source: MGI
- embryonic camera-type eye morphogenesis Source: MGI
- embryonic placenta development Source: MGI
- embryonic process involved in female pregnancy Source: MGI
- embryonic skeletal system development Source: MGI
- enucleate erythrocyte differentiation Source: MGI
- erythrocyte differentiation Source: MGI
- granulocyte differentiation Source: MGI
- in utero embryonic development Source: MGI
- liver development Source: MGI
- lung development Source: MGI
- megakaryocyte differentiation Source: MGI
- monocyte differentiation Source: MGI
- natural killer cell differentiation Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- ossification Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- T cell differentiation Source: MGI
- trophectodermal cell differentiation Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-3232118, SUMOylation of transcription factors |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor Sp3 |
Gene namesi | Name:Sp3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1277166, Sp3 |
VEuPathDBi | HostDB:ENSMUSG00000027109 |
Subcellular locationi
Nucleus
Other locations
- transcription repressor complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047142 | 1 – 783 | Transcription factor Sp3Add BLAST | 783 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 72 | PhosphoserineCombined sources | 1 | |
Cross-linki | 122 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 553 | N6-acetyllysine; alternate1 Publication | 1 | |
Cross-linki | 553 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity | ||
Cross-linki | 553 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 553 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 565 | PhosphoserineCombined sources | 1 | |
Modified residuei | 568 | PhosphoserineCombined sources | 1 | |
Cross-linki | 595 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 648 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O70494 |
MaxQBi | O70494 |
PaxDbi | O70494 |
PeptideAtlasi | O70494 |
PRIDEi | O70494 |
ProteomicsDBi | 263297 [O70494-1] 263298 [O70494-2] 263299 [O70494-3] |
PTM databases
iPTMneti | O70494 |
PhosphoSitePlusi | O70494 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027109, Expressed in indifferent gonad and 320 other tissues |
Genevisiblei | O70494, MM |
Interactioni
Subunit structurei
Interacts with HLTF; the interaction may be required for basal transcriptional activity of HLTF.
Interacts with HDAC1; the interaction deacetylates SP3 and regulates its transcriptional activity.
Interacts with HDAC2 (preferably the CK2-phosphorylated form); the interaction deacetylates SP3 and regulates its transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3.
Interacts with MEIS2 isoform Meis2D and PBX1 isoform PBX1a (By similarity).
By similarityBinary interactionsi
O70494
With | #Exp. | IntAct |
---|---|---|
Mecp2 [Q9Z2D6] | 2 | EBI-643514,EBI-1188816 |
GO - Molecular functioni
- RNA polymerase II-specific DNA-binding transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 203418, 9 interactors |
IntActi | O70494, 2 interactors |
MINTi | O70494 |
STRINGi | 10090.ENSMUSP00000099750 |
Miscellaneous databases
RNActi | O70494, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 55 | DisorderedSequence analysisAdd BLAST | 55 | |
Regioni | 140 – 239 | Transactivation domain (Gln-rich)By similarityAdd BLAST | 100 | |
Regioni | 302 – 340 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 352 – 501 | Transactivation domain (Gln-rich)By similarityAdd BLAST | 150 | |
Regioni | 536 – 622 | Repressor domainBy similarityAdd BLAST | 87 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 463 – 471 | 9aaTADBy similarity | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 29 – 50 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 320 – 340 | Polar residuesSequence analysisAdd BLAST | 21 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 623 – 647 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 653 – 677 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 683 – 705 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000155099 |
HOGENOMi | CLU_019688_2_1_1 |
InParanoidi | O70494 |
OMAi | TCTQVES |
OrthoDBi | 1085860at2759 |
PhylomeDBi | O70494 |
TreeFami | TF350150 |
Family and domain databases
InterProi | View protein in InterPro IPR030452, SP3 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR23235:SF3, PTHR23235:SF3, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTAPEKPVKQ EEMAALDVDG GGGGGGHGEY LQQQQQQQQQ HGNGAAAAAA
60 70 80 90 100
QDTQPSPLAL LAATCSKIGP PSPGDDDEEA AVAAAAGVPA AAAGATGDLA
110 120 130 140 150
SAQLGGAPNR WEVLSATPTT IKDEAGNLVQ IPGAATSSGQ YVLPLQNLQN
160 170 180 190 200
QQIFSVAPGS DSSNGTVSNV QYQVIPQIQS TDAQQVQIGF TGSSDNGGIN
210 220 230 240 250
QENSQIQIIP GSNQTLLASG TPPANIQNLI PQTGQVQVQG VAIGGSSFPG
260 270 280 290 300
QTQVVANVPL GLPGNITFVP INSVDLDSLG LSGSSQTMTA GINADGHLIN
310 320 330 340 350
TGQAMDSSDN SERTGERVSP DVNETNADTD LFVPTSSSSQ LPVTIDSTGI
360 370 380 390 400
LQQNTNSLTT TSGQVHSSDL QGNYIQSPVS EETQAQNIQV STAQPVVQHL
410 420 430 440 450
QLQDSQQPTS QAQIVQGITP QTIHGVQASG QNISQQALQN LQLQLNPGTF
460 470 480 490 500
LIQAQTVTPS GQITWQTFQV QGVQNLQNLQ IQNTAAQQIT LTPVQTLTLG
510 520 530 540 550
QVAAGGALTS TPVSLSTGQL PNLQTVTVNS IDSTGIQLHP GENADSPADI
560 570 580 590 600
RIKEEEPDPE EWQLSGDSTL NTNDLTHLRV QVVDEEGDQQ HQEGKRLRRV
610 620 630 640 650
ACTCPNCKEG GGRGTNLGKK KQHICHIPGC GKVYGKTSHL RAHLRWHSGE
660 670 680 690 700
RPFICNWMFC GKRFTRSDEL QRHRRTHTGE KKFVCPECSK RFMRSDHLAK
710 720 730 740 750
HIKTHQNKKV IHSSSTVLAS VEAGRDDALI TAGGTTLILA NIQQGSVSGI
760 770 780
GTVNTSATSN QDILTNTEIP LQLVTVSGNE TME
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 231 – 233 | PQT → TRP in AAH27797 (PubMed:15489334).Curated | 3 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016782 | 1 – 64 | Missing in isoform 3. 1 PublicationAdd BLAST | 64 | |
Alternative sequenceiVSP_016783 | 52 – 95 | Missing in isoform 2. 1 PublicationAdd BLAST | 44 | |
Alternative sequenceiVSP_016784 | 65 – 94 | CSKIG…AAAAG → MKEDRAAIAGRRRRGRRSCA HDEKTAADKR in isoform 3. 1 PublicationAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004607 mRNA Translation: BAC25090.1 AK132702 mRNA Translation: BAE21310.1 Different initiation. AL844840 Genomic DNA No translation available. BC027797 mRNA Translation: AAH27797.2 Different initiation. BC079874 mRNA Translation: AAH79874.1 AF062567 mRNA Translation: AAC16322.1 |
CCDSi | CCDS16122.1 [O70494-1] |
RefSeqi | NP_001018052.1, NM_001018042.3 [O70494-1] NP_001091895.1, NM_001098425.1 |
Genome annotation databases
Ensembli | ENSMUST00000066003; ENSMUSP00000065807; ENSMUSG00000027109 [O70494-2] ENSMUST00000102689; ENSMUSP00000099750; ENSMUSG00000027109 [O70494-1] |
GeneIDi | 20687 |
KEGGi | mmu:20687 |
UCSCi | uc008kca.2, mouse [O70494-1] uc008kcb.2, mouse [O70494-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004607 mRNA Translation: BAC25090.1 AK132702 mRNA Translation: BAE21310.1 Different initiation. AL844840 Genomic DNA No translation available. BC027797 mRNA Translation: AAH27797.2 Different initiation. BC079874 mRNA Translation: AAH79874.1 AF062567 mRNA Translation: AAC16322.1 |
CCDSi | CCDS16122.1 [O70494-1] |
RefSeqi | NP_001018052.1, NM_001018042.3 [O70494-1] NP_001091895.1, NM_001098425.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 203418, 9 interactors |
IntActi | O70494, 2 interactors |
MINTi | O70494 |
STRINGi | 10090.ENSMUSP00000099750 |
PTM databases
iPTMneti | O70494 |
PhosphoSitePlusi | O70494 |
Proteomic databases
EPDi | O70494 |
MaxQBi | O70494 |
PaxDbi | O70494 |
PeptideAtlasi | O70494 |
PRIDEi | O70494 |
ProteomicsDBi | 263297 [O70494-1] 263298 [O70494-2] 263299 [O70494-3] |
Protocols and materials databases
Antibodypediai | 3887, 408 antibodies from 33 providers |
DNASUi | 20687 |
Genome annotation databases
Ensembli | ENSMUST00000066003; ENSMUSP00000065807; ENSMUSG00000027109 [O70494-2] ENSMUST00000102689; ENSMUSP00000099750; ENSMUSG00000027109 [O70494-1] |
GeneIDi | 20687 |
KEGGi | mmu:20687 |
UCSCi | uc008kca.2, mouse [O70494-1] uc008kcb.2, mouse [O70494-2] |
Organism-specific databases
CTDi | 6670 |
MGIi | MGI:1277166, Sp3 |
VEuPathDBi | HostDB:ENSMUSG00000027109 |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000155099 |
HOGENOMi | CLU_019688_2_1_1 |
InParanoidi | O70494 |
OMAi | TCTQVES |
OrthoDBi | 1085860at2759 |
PhylomeDBi | O70494 |
TreeFami | TF350150 |
Enzyme and pathway databases
Reactomei | R-MMU-3232118, SUMOylation of transcription factors |
Miscellaneous databases
BioGRID-ORCSi | 20687, 12 hits in 66 CRISPR screens |
ChiTaRSi | Sp3, mouse |
PROi | PR:O70494 |
RNActi | O70494, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027109, Expressed in indifferent gonad and 320 other tissues |
Genevisiblei | O70494, MM |
Family and domain databases
InterProi | View protein in InterPro IPR030452, SP3 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR23235:SF3, PTHR23235:SF3, 1 hit |
Pfami | View protein in Pfam PF00096, zf-C2H2, 3 hits |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | SP3_MOUSE | |
Accessioni | O70494Primary (citable) accession number: O70494 Secondary accession number(s): A2AQK9 Q8K378 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 9, 2003 |
Last sequence update: | January 10, 2006 | |
Last modified: | February 23, 2022 | |
This is version 180 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families