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Protein

Transcription factor Sp3

Gene

Sp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri623 – 647C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri653 – 677C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri683 – 705C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3232118 SUMOylation of transcription factors

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Sp3
Gene namesi
Name:Sp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1277166 Sp3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000471421 – 783Transcription factor Sp3Add BLAST783

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphoserineCombined sources1
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei553N6-acetyllysine; alternate1 Publication1
Cross-linki553Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki553Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki553Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei565PhosphoserineCombined sources1
Modified residuei568PhosphoserineCombined sources1
Cross-linki595Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei648PhosphoserineBy similarity1

Post-translational modificationi

Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-553 also control transcriptional activity (By similarity).By similarity
Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-553 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-553 represses the transactivation activity, except for the largest isoform which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-553 also control transcriptional activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO70494
PaxDbiO70494
PeptideAtlasiO70494
PRIDEiO70494

PTM databases

iPTMnetiO70494
PhosphoSitePlusiO70494

Expressioni

Gene expression databases

BgeeiENSMUSG00000027109 Expressed in 301 organ(s), highest expression level in indifferent gonad
CleanExiMM_SP3
GenevisibleiO70494 MM

Interactioni

Subunit structurei

Interacts with HLTF; the interaction may be required for basal transcriptional activity of HLTF. Interacts with HDAC1; the interaction deacetylates SP3 and regulates its transcriptional activity. Interacts with HDAC2 (preferably the CK2-phosphorylated form); the interaction deacetylates SP3 and regulates its transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. Interacts with MEIS2 isoform Meis2D and PBX1 isoform PBX1a (By similarity).By similarity

Protein-protein interaction databases

BioGridi203418, 7 interactors
IntActiO70494, 1 interactor
STRINGi10090.ENSMUSP00000099750

Structurei

3D structure databases

ProteinModelPortaliO70494
SMRiO70494
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 239Transactivation domain (Gln-rich)By similarityAdd BLAST100
Regioni352 – 501Transactivation domain (Gln-rich)By similarityAdd BLAST150
Regioni536 – 622Repressor domainBy similarityAdd BLAST87

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi20 – 28Poly-Gly9
Compositional biasi32 – 40Poly-Gln9
Compositional biasi45 – 102Ala-richAdd BLAST58
Compositional biasi336 – 339Poly-Ser4

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri623 – 647C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri653 – 677C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri683 – 705C2H2-type 3PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118984
HOGENOMiHOG000234295
HOVERGENiHBG008933
InParanoidiO70494
KOiK09193
OMAiIAQQTIH
OrthoDBiEOG091G0HX6
PhylomeDBiO70494
TreeFamiTF350150

Family and domain databases

InterProiView protein in InterPro
IPR030452 SP3
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR23235:SF3 PTHR23235:SF3, 1 hit
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O70494-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTAPEKPVKQ EEMAALDVDG GGGGGGHGEY LQQQQQQQQQ HGNGAAAAAA
60 70 80 90 100
QDTQPSPLAL LAATCSKIGP PSPGDDDEEA AVAAAAGVPA AAAGATGDLA
110 120 130 140 150
SAQLGGAPNR WEVLSATPTT IKDEAGNLVQ IPGAATSSGQ YVLPLQNLQN
160 170 180 190 200
QQIFSVAPGS DSSNGTVSNV QYQVIPQIQS TDAQQVQIGF TGSSDNGGIN
210 220 230 240 250
QENSQIQIIP GSNQTLLASG TPPANIQNLI PQTGQVQVQG VAIGGSSFPG
260 270 280 290 300
QTQVVANVPL GLPGNITFVP INSVDLDSLG LSGSSQTMTA GINADGHLIN
310 320 330 340 350
TGQAMDSSDN SERTGERVSP DVNETNADTD LFVPTSSSSQ LPVTIDSTGI
360 370 380 390 400
LQQNTNSLTT TSGQVHSSDL QGNYIQSPVS EETQAQNIQV STAQPVVQHL
410 420 430 440 450
QLQDSQQPTS QAQIVQGITP QTIHGVQASG QNISQQALQN LQLQLNPGTF
460 470 480 490 500
LIQAQTVTPS GQITWQTFQV QGVQNLQNLQ IQNTAAQQIT LTPVQTLTLG
510 520 530 540 550
QVAAGGALTS TPVSLSTGQL PNLQTVTVNS IDSTGIQLHP GENADSPADI
560 570 580 590 600
RIKEEEPDPE EWQLSGDSTL NTNDLTHLRV QVVDEEGDQQ HQEGKRLRRV
610 620 630 640 650
ACTCPNCKEG GGRGTNLGKK KQHICHIPGC GKVYGKTSHL RAHLRWHSGE
660 670 680 690 700
RPFICNWMFC GKRFTRSDEL QRHRRTHTGE KKFVCPECSK RFMRSDHLAK
710 720 730 740 750
HIKTHQNKKV IHSSSTVLAS VEAGRDDALI TAGGTTLILA NIQQGSVSGI
760 770 780
GTVNTSATSN QDILTNTEIP LQLVTVSGNE TME
Length:783
Mass (Da):82,362
Last modified:January 10, 2006 - v2
Checksum:iE45A00D566454D61
GO
Isoform 2 (identifier: O70494-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     52-95: Missing.

Show »
Length:739
Mass (Da):78,364
Checksum:i22CEB08B93A699B0
GO
Isoform 3 (identifier: O70494-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: Missing.
     65-94: CSKIGPPSPGDDDEEAAVAAAAGVPAAAAG → MKEDRAAIAGRRRRGRRSCAHDEKTAADKR

Note: No experimental confirmation available.
Show »
Length:719
Mass (Da):76,729
Checksum:i8B16F3B85E282654
GO

Sequence cautioni

The sequence AAH27797 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE21310 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231 – 233PQT → TRP in AAH27797 (PubMed:15489334).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0167821 – 64Missing in isoform 3. 1 PublicationAdd BLAST64
Alternative sequenceiVSP_01678352 – 95Missing in isoform 2. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_01678465 – 94CSKIG…AAAAG → MKEDRAAIAGRRRRGRRSCA HDEKTAADKR in isoform 3. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004607 mRNA Translation: BAC25090.1
AK132702 mRNA Translation: BAE21310.1 Different initiation.
AL844840 Genomic DNA No translation available.
BC027797 mRNA Translation: AAH27797.2 Different initiation.
BC079874 mRNA Translation: AAH79874.1
AF062567 mRNA Translation: AAC16322.1
CCDSiCCDS16122.1 [O70494-1]
RefSeqiNP_001018052.1, NM_001018042.3 [O70494-1]
NP_001091895.1, NM_001098425.1
UniGeneiMm.124328
Mm.446209
Mm.465887

Genome annotation databases

EnsembliENSMUST00000066003; ENSMUSP00000065807; ENSMUSG00000027109 [O70494-2]
ENSMUST00000102689; ENSMUSP00000099750; ENSMUSG00000027109 [O70494-1]
GeneIDi20687
KEGGimmu:20687
UCSCiuc008kca.2 mouse [O70494-1]
uc008kcb.2 mouse [O70494-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004607 mRNA Translation: BAC25090.1
AK132702 mRNA Translation: BAE21310.1 Different initiation.
AL844840 Genomic DNA No translation available.
BC027797 mRNA Translation: AAH27797.2 Different initiation.
BC079874 mRNA Translation: AAH79874.1
AF062567 mRNA Translation: AAC16322.1
CCDSiCCDS16122.1 [O70494-1]
RefSeqiNP_001018052.1, NM_001018042.3 [O70494-1]
NP_001091895.1, NM_001098425.1
UniGeneiMm.124328
Mm.446209
Mm.465887

3D structure databases

ProteinModelPortaliO70494
SMRiO70494
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203418, 7 interactors
IntActiO70494, 1 interactor
STRINGi10090.ENSMUSP00000099750

PTM databases

iPTMnetiO70494
PhosphoSitePlusiO70494

Proteomic databases

MaxQBiO70494
PaxDbiO70494
PeptideAtlasiO70494
PRIDEiO70494

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066003; ENSMUSP00000065807; ENSMUSG00000027109 [O70494-2]
ENSMUST00000102689; ENSMUSP00000099750; ENSMUSG00000027109 [O70494-1]
GeneIDi20687
KEGGimmu:20687
UCSCiuc008kca.2 mouse [O70494-1]
uc008kcb.2 mouse [O70494-2]

Organism-specific databases

CTDi6670
MGIiMGI:1277166 Sp3

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00760000118984
HOGENOMiHOG000234295
HOVERGENiHBG008933
InParanoidiO70494
KOiK09193
OMAiIAQQTIH
OrthoDBiEOG091G0HX6
PhylomeDBiO70494
TreeFamiTF350150

Enzyme and pathway databases

ReactomeiR-MMU-3232118 SUMOylation of transcription factors

Miscellaneous databases

PROiPR:O70494
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027109 Expressed in 301 organ(s), highest expression level in indifferent gonad
CleanExiMM_SP3
GenevisibleiO70494 MM

Family and domain databases

InterProiView protein in InterPro
IPR030452 SP3
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR23235:SF3 PTHR23235:SF3, 1 hit
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 3 hits
SUPFAMiSSF57667 SSF57667, 2 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSP3_MOUSE
AccessioniPrimary (citable) accession number: O70494
Secondary accession number(s): A2AQK9
, Q68FF2, Q8CF64, Q8K378
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: January 10, 2006
Last modified: November 7, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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