UniProtKB - O70481 (UBR1_MOUSE)
Protein
E3 ubiquitin-protein ligase UBR1
Gene
Ubr1
Organism
Mus musculus (Mouse)
Status
Functioni
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth (By similarity).By similarity3 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 97 – 168 | UBR-typePROSITE-ProRule annotationAdd BLAST | 72 | |
Zinc fingeri | 1101 – 1204 | RING-type; atypicalAdd BLAST | 104 |
GO - Molecular functioni
- leucine binding Source: MGI
- ubiquitin protein ligase activity Source: GO_Central
- ubiquitin-protein transferase activity Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- cellular response to leucine Source: UniProtKB
- negative regulation of TOR signaling Source: UniProtKB
- protein ubiquitination Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: MGI
- ubiquitin-dependent protein catabolic process via the N-end rule pathway Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase UBR1 (EC:2.3.2.27)Alternative name(s): N-recognin-1 RING-type E3 ubiquitin transferase UBR1 Ubiquitin-protein ligase E3-alpha-1 Ubiquitin-protein ligase E3-alpha-I |
Gene namesi | Name:Ubr1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1277977, Ubr1 |
Subcellular locationi
Cytosol
- cytosol By similarity
Cytosol
- cytosol Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
- proteasome complex Source: MGI
- ubiquitin ligase complex Source: MGI
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mice are viable and fertile, but show a decreased mass of skeletal muscle and adipose tissue. They have exocrine pancreatic insufficiency with impaired stimulus-secretion coupling and increased susceptibiliy to pancreatic injury. UBR1 and UBR2 double knockout embryos die at midgestation, with defects in neurogenesis and cardiovascular development. These defects included reduced proliferation as well as precocious migration and differentiation of neural progenitor cells.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000056137 | 2 – 1757 | E3 ubiquitin-protein ligase UBR1Add BLAST | 1756 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 1182 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O70481 |
MaxQBi | O70481 |
PaxDbi | O70481 |
PeptideAtlasi | O70481 |
PRIDEi | O70481 |
PTM databases
iPTMneti | O70481 |
PhosphoSitePlusi | O70481 |
Expressioni
Tissue specificityi
Present in skeletal muscle and liver (at protein level). Broadly expressed, with highest levels in skeletal muscle and heart. Expressed in acinar cells of the pancreas. In testes, expressed primarily in spermatogonia.5 Publications
Developmental stagei
Expressed in limb buds at 9.5-11.5 dpc.1 Publication
Inductioni
In models of cancer cachexia, induced in muscle during the progression of wasting.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000027272, Expressed in cumulus cell and 288 other tissues |
Genevisiblei | O70481, MM |
Interactioni
Subunit structurei
Interacts with RECQL4.
By similarityProtein-protein interaction databases
BioGRIDi | 204422, 8 interactors |
ELMi | O70481 |
IntActi | O70481, 2 interactors |
STRINGi | 10090.ENSMUSP00000028728 |
Miscellaneous databases
RNActi | O70481, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 864 – 869 | Poly-Pro | 6 |
Domaini
The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.
The UBR-type zinc finger forms a pocket that mediates recognition of type 1 N-degrons. It exhibits preference for Arginine in first position, has poor affinity for histidine, and doesn't bind acetylated peptides (By similarity).By similarity
Sequence similaritiesi
Belongs to the UBR1 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 97 – 168 | UBR-typePROSITE-ProRule annotationAdd BLAST | 72 | |
Zinc fingeri | 1101 – 1204 | RING-type; atypicalAdd BLAST | 104 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1140, Eukaryota |
GeneTreei | ENSGT00950000183075 |
HOGENOMi | CLU_001801_2_0_1 |
InParanoidi | O70481 |
OMAi | AEMLVVC |
OrthoDBi | 81415at2759 |
TreeFami | TF323875 |
Family and domain databases
Gene3Di | 3.30.1390.10, 1 hit |
InterProi | View protein in InterPro IPR003769, ClpS_core IPR044046, E3_ligase_UBR-like_C IPR014719, Ribosomal_L7/12_C/ClpS-like IPR039164, UBR1-like IPR036390, WH_DNA-bd_sf IPR003126, Znf_UBR |
PANTHERi | PTHR21497, PTHR21497, 1 hit |
Pfami | View protein in Pfam PF02617, ClpS, 1 hit PF18995, PRT6_C, 1 hit PF02207, zf-UBR, 1 hit |
SMARTi | View protein in SMART SM00396, ZnF_UBR1, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF54736, SSF54736, 1 hit |
PROSITEi | View protein in PROSITE PS51157, ZF_UBR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O70481-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADEEMDGAE RMDVSPEPPL APQRPASWWD QQVDFYTAFL HHLAQLVPEI
60 70 80 90 100
YFAEMDPDLE KQEESVQMSI LTPLEWYLFG EDPDICLEKL KHSGAFQLCG
110 120 130 140 150
KVFKSGETTY SCRDCAIDPT CVLCMDCFQS SVHKNHRYKM HTSTGGGFCD
160 170 180 190 200
CGDTEAWKTG PFCVDHEPGR AGTTKESLHC PLNEEVIAQA RRIFPSVIKY
210 220 230 240 250
IVEMTIWEEE KELPPELQIR EKNERYYCVL FNDEHHSYDH VIYSLQRALD
260 270 280 290 300
CELAEAQLHT TAIDKEGRRA VKAGVYATCQ EAKEDIKSHS ENVSQHPLHV
310 320 330 340 350
EVLHSVVMAH QKFALRLGSW MNKIMSYSSD FRQIFCQACL VEEPGSENPC
360 370 380 390 400
LISRLMLWDA KLYKGARKIL HELIFSSFFM EMEYKKLFAM EFVKYYKQLQ
410 420 430 440 450
KEYISDDHER SISITALSVQ MLTVPTLARH LIEEQNVISV ITETLLEVLP
460 470 480 490 500
EYLDRNNKFN FQGYSQDKLG RVYAVICDLK YILISKPVIW TERLRAQFLE
510 520 530 540 550
GFRSFLKILT CMQGMEEIRR QVGQHIEVDP DWEAAIAIQM QLKNILLMFQ
560 570 580 590 600
EWCACDEDLL LVAYKECHKA VMRCSTNFMS STKTVVQLCG HSLETKSYKV
610 620 630 640 650
SEDLVSIHLP LSRTLAGLHV RLSRLGAISR LHEFVPFDGF QVEVLVEYPL
660 670 680 690 700
RCLVLVAQVV AEMWRRNGLS LISQVFYYQD VKCREEMYDK DIIMLQIGAS
710 720 730 740 750
IMDPNKFLLL VLQRYELTDA FNKTISTKDQ DLIKQYNTLI EEMLQVLIYI
760 770 780 790 800
VGERYVPGVG NVTREEVIMR EITHLLCIEP MPHSAIARNL PENENNETGL
810 820 830 840 850
ENVINKVATF KKPGVSGHGV YELKDESLKD FNMYFYHYSK TQHSKAEHMQ
860 870 880 890 900
KKRRKQENKD EALPPPPPPE FCPAFSKVVN LLSCDVMMYI LRTIFERAVD
910 920 930 940 950
MESNLWTEGM LQMAFHILAL GLLEEKQQLQ KAPEEEVAFD FYHKASRLGS
960 970 980 990 1000
SAMNAQNIQM LLEKLKGIPQ LESQKDMITW ILQMFDTVKR LREKSCLVVA
1010 1020 1030 1040 1050
TTSGLECVKS EEITHDKEKA ERKRKAEAAR LHRQKIMAQM SALQKNFIET
1060 1070 1080 1090 1100
HKLMYDNTSE VTGKEDSIME EESTSAVSEA SRIALGPKRG PAVTEKEVLT
1110 1120 1130 1140 1150
CILCQEEQEV KLENNAMVLS ACVQKSTALT QHRGKPVDHL GETLDPLFMD
1160 1170 1180 1190 1200
PDLAHGTYTG SCGHVMHAVC WQKYFEAVQL SSQQRIHVDL FDLESGEYLC
1210 1220 1230 1240 1250
PLCKSLCNTV IPIIPLQPQK INSENAEALA QLLTLARWIQ TVLARISGYN
1260 1270 1280 1290 1300
IKHAKGEAPA VPVLFNQGMG DSTFEFHSIL SFGVQSSVKY SNSIKEMVIL
1310 1320 1330 1340 1350
FATTIYRIGL KVPPDELDPR VPMMTWSTCA FTIQAIENLL GDEGKPLFGA
1360 1370 1380 1390 1400
LQNRQHNGLK ALMQFAVAQR TTCPQVLIHK HLARLLSVIL PNLQSENTPG
1410 1420 1430 1440 1450
LLSVDLFHVL VGAVLAFPSL YWDDTVDLQP SPLSSSYNHL YLFHLITMAH
1460 1470 1480 1490 1500
MLQILLTTDT DLSSGPPLAE GEEDSEEARC ASAFFVEVSQ HTDGLAGCGA
1510 1520 1530 1540 1550
PGWYLWLSLR NGITPYLRCA ALLFHYLLGV APPEELFANS AEGEFSALCS
1560 1570 1580 1590 1600
YLSLPTNLFL LFQEYWDTIR PLLQRWCGDP ALLKSLKQKS AVVRYPRKRN
1610 1620 1630 1640 1650
SLIELPEDYS CLLNQASHFR CPRSADDERK HPVLCLFCGA ILCSQNICCQ
1660 1670 1680 1690 1700
EIVNGEEVGA CVFHALHCGA GVCIFLKIRE CRVVLVEGKA RGCAYPAPYL
1710 1720 1730 1740 1750
DEYGETDPGL KRGNPLHLSR ERYRKLHLVW QQHCIIEEIA RSQETNQMLF
GFNWQLL
Sequence cautioni
The sequence AAH90969 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 639 | G → S in AAC40165 (PubMed:9653112).Curated | 1 | |
Sequence conflicti | 639 | G → S in BAC40933 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 888 | M → I in AAC40165 (PubMed:9653112).Curated | 1 | |
Sequence conflicti | 901 | M → T in AAC40165 (PubMed:9653112).Curated | 1 | |
Sequence conflicti | 964 | K → R in AAC40165 (PubMed:9653112).Curated | 1 | |
Sequence conflicti | 973 | S → G in AAC40165 (PubMed:9653112).Curated | 1 | |
Sequence conflicti | 1008 | V → I in AAC40165 (PubMed:9653112).Curated | 1 | |
Sequence conflicti | 1357 | N → S in AAC40165 (PubMed:9653112).Curated | 1 | |
Sequence conflicti | 1371 | T → A in AAC40165 (PubMed:9653112).Curated | 1 | |
Sequence conflicti | 1464 | S → P in AAC40165 (PubMed:9653112).Curated | 1 | |
Sequence conflicti | 1496 | A → T in AAC40165 (PubMed:9653112).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061555 mRNA Translation: AAC40165.1 AH006182 Genomic DNA Translation: AAC23678.1 AL844548 Genomic DNA No translation available. AL935168 Genomic DNA No translation available. AK078173 mRNA Translation: BAC37160.1 AK089616 mRNA Translation: BAC40933.1 BC090969 mRNA Translation: AAH90969.1 Sequence problems. |
CCDSi | CCDS16628.1 |
PIRi | T14318 |
RefSeqi | NP_033487.2, NM_009461.2 |
Genome annotation databases
Ensembli | ENSMUST00000028728; ENSMUSP00000028728; ENSMUSG00000027272 |
GeneIDi | 22222 |
KEGGi | mmu:22222 |
UCSCi | uc008lxb.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061555 mRNA Translation: AAC40165.1 AH006182 Genomic DNA Translation: AAC23678.1 AL844548 Genomic DNA No translation available. AL935168 Genomic DNA No translation available. AK078173 mRNA Translation: BAC37160.1 AK089616 mRNA Translation: BAC40933.1 BC090969 mRNA Translation: AAH90969.1 Sequence problems. |
CCDSi | CCDS16628.1 |
PIRi | T14318 |
RefSeqi | NP_033487.2, NM_009461.2 |
3D structure databases
SMRi | O70481 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204422, 8 interactors |
ELMi | O70481 |
IntActi | O70481, 2 interactors |
STRINGi | 10090.ENSMUSP00000028728 |
PTM databases
iPTMneti | O70481 |
PhosphoSitePlusi | O70481 |
Proteomic databases
EPDi | O70481 |
MaxQBi | O70481 |
PaxDbi | O70481 |
PeptideAtlasi | O70481 |
PRIDEi | O70481 |
Protocols and materials databases
Antibodypediai | 23750, 182 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028728; ENSMUSP00000028728; ENSMUSG00000027272 |
GeneIDi | 22222 |
KEGGi | mmu:22222 |
UCSCi | uc008lxb.1, mouse |
Organism-specific databases
CTDi | 197131 |
MGIi | MGI:1277977, Ubr1 |
Phylogenomic databases
eggNOGi | KOG1140, Eukaryota |
GeneTreei | ENSGT00950000183075 |
HOGENOMi | CLU_001801_2_0_1 |
InParanoidi | O70481 |
OMAi | AEMLVVC |
OrthoDBi | 81415at2759 |
TreeFami | TF323875 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 22222, 0 hits in 17 CRISPR screens |
ChiTaRSi | Ubr1, mouse |
PROi | PR:O70481 |
RNActi | O70481, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027272, Expressed in cumulus cell and 288 other tissues |
Genevisiblei | O70481, MM |
Family and domain databases
Gene3Di | 3.30.1390.10, 1 hit |
InterProi | View protein in InterPro IPR003769, ClpS_core IPR044046, E3_ligase_UBR-like_C IPR014719, Ribosomal_L7/12_C/ClpS-like IPR039164, UBR1-like IPR036390, WH_DNA-bd_sf IPR003126, Znf_UBR |
PANTHERi | PTHR21497, PTHR21497, 1 hit |
Pfami | View protein in Pfam PF02617, ClpS, 1 hit PF18995, PRT6_C, 1 hit PF02207, zf-UBR, 1 hit |
SMARTi | View protein in SMART SM00396, ZnF_UBR1, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF54736, SSF54736, 1 hit |
PROSITEi | View protein in PROSITE PS51157, ZF_UBR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBR1_MOUSE | |
Accessioni | O70481Primary (citable) accession number: O70481 Secondary accession number(s): A2AQ54 Q8C5K3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families