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Protein

Vesicle-associated membrane protein 4

Gene

Vamp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei87 – 88(Microbial infection) Cleavage; by C.botulinum neurotoxin type X (BoNT/X)1 Publication2

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-6811440 Retrograde transport at the Trans-Golgi-Network
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 4
Short name:
VAMP-4
Gene namesi
Name:Vamp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1858730 Vamp4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 118CytoplasmicSequence analysisAdd BLAST118
Transmembranei119 – 139Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini140 – 141VesicularSequence analysis2

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067321 – 141Vesicle-associated membrane protein 4Add BLAST141

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineCombined sources1
Modified residuei30PhosphoserineCombined sources1

Post-translational modificationi

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which hydrolyzes the 87-Arg-|-Ser-88 bond and probably inhibits neurotransmitter release (PubMed:28770820). It remains unknown whether BoNT/X is ever produced, or what organisms it targets.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO70480
MaxQBiO70480
PaxDbiO70480
PeptideAtlasiO70480
PRIDEiO70480

PTM databases

iPTMnetiO70480
PhosphoSitePlusiO70480
SwissPalmiO70480

Expressioni

Gene expression databases

BgeeiENSMUSG00000026696 Expressed in 288 organ(s), highest expression level in decidua
CleanExiMM_VAMP4
ExpressionAtlasiO70480 baseline and differential
GenevisibleiO70480 MM

Interactioni

Subunit structurei

Identified in a complex containing STX6, STX12, VAMP4 and VTI1A (PubMed:17159904). Interacts with BAIAP3; this interaction is increased in the presence of calcium (By similarity).By similarity1 Publication

Protein-protein interaction databases

CORUMiO70480
IntActiO70480, 3 interactors
MINTiO70480
STRINGi10090.ENSMUSP00000051544

Structurei

Secondary structure

1141
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO70480
SMRiO70480
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO70480

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 112v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG061695
InParanoidiO70480
KOiK08513
OMAiWRGCKMK
OrthoDBiEOG091G0MPE
PhylomeDBiO70480

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O70480-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN
60 70 80 90 100
DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN
110 120 130 140
RSKQLRRQMW WRGCKIKAIM ALAAAILLLM IIILIVVKFR T
Length:141
Mass (Da):16,353
Last modified:August 1, 1998 - v1
Checksum:i556CCCB8213CF91E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8BSN6Q8BSN6_MOUSE
Vesicle-associated membrane protein...
Vamp4 mCG_22688
141Annotation score:
A0A0A6YY66A0A0A6YY66_MOUSE
Vesicle-associated membrane protein...
Vamp4
100Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113G → V in BAB27774 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061516 mRNA Translation: AAC15776.1
AK011678 mRNA Translation: BAB27774.1
AK018344 mRNA Translation: BAB31171.1
CCDSiCCDS83616.1
RefSeqiNP_001334054.1, NM_001347125.1
XP_006496997.1, XM_006496934.2
UniGeneiMm.10699

Genome annotation databases

EnsembliENSMUST00000135241; ENSMUSP00000116376; ENSMUSG00000026696
ENSMUST00000150040; ENSMUSP00000115133; ENSMUSG00000026696
ENSMUST00000155003; ENSMUSP00000114172; ENSMUSG00000026696
GeneIDi53330
KEGGimmu:53330
UCSCiuc007dgk.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061516 mRNA Translation: AAC15776.1
AK011678 mRNA Translation: BAB27774.1
AK018344 mRNA Translation: BAB31171.1
CCDSiCCDS83616.1
RefSeqiNP_001334054.1, NM_001347125.1
XP_006496997.1, XM_006496934.2
UniGeneiMm.10699

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50A47-117[»]
ProteinModelPortaliO70480
SMRiO70480
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiO70480
IntActiO70480, 3 interactors
MINTiO70480
STRINGi10090.ENSMUSP00000051544

PTM databases

iPTMnetiO70480
PhosphoSitePlusiO70480
SwissPalmiO70480

Proteomic databases

EPDiO70480
MaxQBiO70480
PaxDbiO70480
PeptideAtlasiO70480
PRIDEiO70480

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000135241; ENSMUSP00000116376; ENSMUSG00000026696
ENSMUST00000150040; ENSMUSP00000115133; ENSMUSG00000026696
ENSMUST00000155003; ENSMUSP00000114172; ENSMUSG00000026696
GeneIDi53330
KEGGimmu:53330
UCSCiuc007dgk.1 mouse

Organism-specific databases

CTDi8674
MGIiMGI:1858730 Vamp4

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
GeneTreeiENSGT00550000074449
HOGENOMiHOG000042711
HOVERGENiHBG061695
InParanoidiO70480
KOiK08513
OMAiWRGCKMK
OrthoDBiEOG091G0MPE
PhylomeDBiO70480

Enzyme and pathway databases

ReactomeiR-MMU-6811440 Retrograde transport at the Trans-Golgi-Network
R-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

EvolutionaryTraceiO70480
PROiPR:O70480
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026696 Expressed in 288 organ(s), highest expression level in decidua
CleanExiMM_VAMP4
ExpressionAtlasiO70480 baseline and differential
GenevisibleiO70480 MM

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS00417 SYNAPTOBREVIN, 1 hit
PS50892 V_SNARE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP4_MOUSE
AccessioniPrimary (citable) accession number: O70480
Secondary accession number(s): Q9D095
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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