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Protein

BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2

Gene

Tnfaip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
Alternative name(s):
BTB/POZ domain-containing protein TNFAIP1
Tumor necrosis factor, alpha-induced protein 1, endothelial
Gene namesi
Name:Tnfaip1
Synonyms:Edp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:104961 Tnfaip1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003312471 – 316BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2Add BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei278PhosphoserineBy similarity1
Modified residuei280Phosphoserine; by CK2By similarity1

Post-translational modificationi

Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO70479
MaxQBiO70479
PaxDbiO70479
PeptideAtlasiO70479
PRIDEiO70479

PTM databases

iPTMnetiO70479
PhosphoSitePlusiO70479

Expressioni

Gene expression databases

BgeeiENSMUSG00000017615 Expressed in 303 organ(s), highest expression level in metanephric S-shaped body
GenevisibleiO70479 MM

Interactioni

Subunit structurei

Component of the BCR(TNFAIP1) E3 ubiquitin ligase complex, at least composed of CUL3, TNFAIP1/BACURD2 and RBX1. Interacts with RHOA; with a preference for RhoA-GDP. Interacts with RHOB. Interacts with PCNA. Interacts with CSNK2B (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

MINTiO70479
STRINGi10090.ENSMUSP00000017759

Structurei

3D structure databases

ProteinModelPortaliO70479
SMRiO70479
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 96BTBPROSITE-ProRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the BACURD family.Curated

Phylogenomic databases

eggNOGiKOG2716 Eukaryota
ENOG410XQYZ LUCA
GeneTreeiENSGT00920000149007
HOGENOMiHOG000294088
HOVERGENiHBG052219
InParanoidiO70479
KOiK15074
OMAiREMSGDT
OrthoDBiEOG091G0L1Y
PhylomeDBiO70479
TreeFamiTF315649

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR003131 T1-type_BTB
PfamiView protein in Pfam
PF02214 BTB_2, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit

Sequencei

Sequence statusi: Complete.

O70479-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGDTCLCPA SGAKPKISGF KGGGLGNKYV QLNVGGSLYY TTVRALTRHD
60 70 80 90 100
TMLKAMFSGR MEVLTDKEGW ILIDRCGKHF GTILNYLRDD TITLPQSRQE
110 120 130 140 150
IQELMAEAKY YLIQGLVSTC QTALQDKKDS YQPVCNIPII TSLREEDRLI
160 170 180 190 200
ESSTKPVVKL LYNRSNNKYS YTSNSDDHLL KNIELFDKLS LRFNGRVLFI
210 220 230 240 250
KDVIGDEICC WSFYGQGRKL AEVCCTSIVY ATEKKQTKVE FPEARIYEET
260 270 280 290 300
LNVLLYETPR VPDNSLLEAT SRSRSQASPS EDEDTFELRD RVRRIHVKRY
310
STYDDRQLGH QSTHRD
Length:316
Mass (Da):36,134
Last modified:August 1, 1998 - v1
Checksum:i9D8E141B0BE311EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94L → H in BAC28668 (PubMed:16141072).Curated1
Sequence conflicti100E → G in BAE40313 (PubMed:16141072).Curated1
Sequence conflicti245R → G in BAE28152 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061346
, AF061341, AF061342, AF061343, AF061344, AF061345 Genomic DNA Translation: AAC78826.1
AK004593 mRNA Translation: BAB23395.1
AK034305 mRNA Translation: BAC28668.1
AK079869 mRNA Translation: BAC37770.1
AK147809 mRNA Translation: BAE28152.1
AK168386 mRNA Translation: BAE40313.1
AK168704 mRNA Translation: BAE40546.1
AL591177 Genomic DNA Translation: CAI25552.1
BC003906 mRNA Translation: AAH03906.1
CCDSiCCDS25110.1
RefSeqiNP_001152864.1, NM_001159392.1
NP_033421.3, NM_009395.4
UniGeneiMm.386774

Genome annotation databases

EnsembliENSMUST00000017759; ENSMUSP00000017759; ENSMUSG00000017615
ENSMUST00000108277; ENSMUSP00000103912; ENSMUSG00000017615
GeneIDi21927
KEGGimmu:21927
UCSCiuc007kjq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061346
, AF061341, AF061342, AF061343, AF061344, AF061345 Genomic DNA Translation: AAC78826.1
AK004593 mRNA Translation: BAB23395.1
AK034305 mRNA Translation: BAC28668.1
AK079869 mRNA Translation: BAC37770.1
AK147809 mRNA Translation: BAE28152.1
AK168386 mRNA Translation: BAE40313.1
AK168704 mRNA Translation: BAE40546.1
AL591177 Genomic DNA Translation: CAI25552.1
BC003906 mRNA Translation: AAH03906.1
CCDSiCCDS25110.1
RefSeqiNP_001152864.1, NM_001159392.1
NP_033421.3, NM_009395.4
UniGeneiMm.386774

3D structure databases

ProteinModelPortaliO70479
SMRiO70479
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiO70479
STRINGi10090.ENSMUSP00000017759

PTM databases

iPTMnetiO70479
PhosphoSitePlusiO70479

Proteomic databases

EPDiO70479
MaxQBiO70479
PaxDbiO70479
PeptideAtlasiO70479
PRIDEiO70479

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017759; ENSMUSP00000017759; ENSMUSG00000017615
ENSMUST00000108277; ENSMUSP00000103912; ENSMUSG00000017615
GeneIDi21927
KEGGimmu:21927
UCSCiuc007kjq.2 mouse

Organism-specific databases

CTDi7126
MGIiMGI:104961 Tnfaip1

Phylogenomic databases

eggNOGiKOG2716 Eukaryota
ENOG410XQYZ LUCA
GeneTreeiENSGT00920000149007
HOGENOMiHOG000294088
HOVERGENiHBG052219
InParanoidiO70479
KOiK15074
OMAiREMSGDT
OrthoDBiEOG091G0L1Y
PhylomeDBiO70479
TreeFamiTF315649

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

ChiTaRSiTnfaip1 mouse
PROiPR:O70479
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017615 Expressed in 303 organ(s), highest expression level in metanephric S-shaped body
GenevisibleiO70479 MM

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR003131 T1-type_BTB
PfamiView protein in Pfam
PF02214 BTB_2, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBACD2_MOUSE
AccessioniPrimary (citable) accession number: O70479
Secondary accession number(s): Q3TH85
, Q3UGQ5, Q8BV61, Q8BZK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 1, 1998
Last modified: September 12, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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