UniProtKB - O70473 (AK1A1_CRIGR)
Aldo-keto reductase family 1 member A1
AKR1A1
Functioni
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde (By similarity).
Plays an important role by catalyzing the reduction of D-glucuronic acid and D-glucurono-gamma-lactone. Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3-deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity).
Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs (By similarity).
Displays no reductase activity towards retinoids (By similarity).
By similarityCatalytic activityi
- EC:1.1.1.2By similarity
- EC:1.1.1.19By similarity
- EC:1.1.1.20By similarity
- EC:1.1.1.54By similarity
- EC:1.1.1.372By similarity
- EC:1.1.1.372By similarity
- EC:1.1.1.33By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 44 | NADPBy similarity | 1 | |
Active sitei | 49 | Proton donorBy similarity | 1 | |
Sitei | 79 | Lowers pKa of active site TyrBy similarity | 1 | |
Binding sitei | 112 | SubstrateBy similarity | 1 | |
Binding sitei | 183 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 22 | NADPBy similarity | 3 | |
Nucleotide bindingi | 161 – 162 | NADPBy similarity | 2 | |
Nucleotide bindingi | 209 – 216 | NADPBy similarity | 8 | |
Nucleotide bindingi | 260 – 272 | NADPBy similarityAdd BLAST | 13 |
GO - Molecular functioni
- alditol:NADP+ 1-oxidoreductase activity Source: Ensembl
- allyl-alcohol dehydrogenase activity Source: UniProtKB-EC
- glucuronolactone reductase activity Source: UniProtKB
- glycerol dehydrogenase [NADP+] activity Source: RHEA
- L-glucuronate reductase activity Source: UniProtKB
- methylglyoxal reductase (NADPH-dependent, acetol producing) Source: RHEA
- mevaldate reductase (NADPH) activity Source: UniProtKB-EC
GO - Biological processi
- aldehyde catabolic process Source: Ensembl
- cellular detoxification of aldehyde Source: UniProtKB
- daunorubicin metabolic process Source: Ensembl
- D-glucuronate catabolic process Source: Ensembl
- doxorubicin metabolic process Source: Ensembl
- L-ascorbic acid biosynthetic process Source: Ensembl
- lipid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid metabolism |
Ligand | NADP |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:AKR1A1 Synonyms:ALR |
Organismi | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) |
Taxonomic identifieri | 10029 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Cricetidae › Cricetinae › Cricetulus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity
Cytoplasm and Cytosol
- cytosol By similarity
Cytosol
- cytosol Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: UniProtKB
Other locations
- synapse Source: Ensembl
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000124616 | 1 – 324 | Aldo-keto reductase family 1 member A1Add BLAST | 324 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | PhosphoserineBy similarity | 1 | |
Modified residuei | 37 | PhosphoserineBy similarity | 1 | |
Modified residuei | 126 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 126 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 144 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 210 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinInteractioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 10029.XP_007620230.1 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000156539 |
OMAi | WRHPDEP |
Family and domain databases
CDDi | cd19106, AKR_AKR1A1-4, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR044481, AKR1A IPR018170, Aldo/ket_reductase_CS IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MASCVLLHTG QKMPLIGLGT WKSNPGQVKA AIKYALSVGY RHIDCAAVYG
60 70 80 90 100
NEIEIGEALK ENVGPGKAVP REELFVTSKL WNTKHHPEDV EAALRKTLAD
110 120 130 140 150
LQLEYLDLYL MHWPYAFERG DNPFPKNDDG TIRYDSTHYK ETWKALEALV
160 170 180 190 200
AKGLVKALGL SNFNSRQIDD ILSVASVRPA VLQVECHPYL AQNELIAHCQ
210 220 230 240 250
ARGLEVTAYS PLGSSDRAWR HPDEPVLLEE PVVLALAEKH GRSPAQILLR
260 270 280 290 300
WQVQRKVVCI PKSITPSRIL QNIQVFDFTF SPEEMKQLDA LNKHWRYIVP
310 320
MITVDGKSVP RDAGHPLYPF NDPY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JH000068 Genomic DNA No translation available. AF060820 mRNA Translation: AAC15760.1 |
RefSeqi | XP_003497420.1, XM_003497372.3 |
Genome annotation databases
Ensembli | ENSCGRT00001030375; ENSCGRP00001026129; ENSCGRG00001023544 |
GeneIDi | 100689106 |
KEGGi | cge:100689106 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JH000068 Genomic DNA No translation available. AF060820 mRNA Translation: AAC15760.1 |
RefSeqi | XP_003497420.1, XM_003497372.3 |
3D structure databases
AlphaFoldDBi | O70473 |
SMRi | O70473 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10029.XP_007620230.1 |
Genome annotation databases
Ensembli | ENSCGRT00001030375; ENSCGRP00001026129; ENSCGRG00001023544 |
GeneIDi | 100689106 |
KEGGi | cge:100689106 |
Organism-specific databases
CTDi | 10327 |
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000156539 |
OMAi | WRHPDEP |
Family and domain databases
CDDi | cd19106, AKR_AKR1A1-4, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR044481, AKR1A IPR018170, Aldo/ket_reductase_CS IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AK1A1_CRIGR | |
Accessioni | O70473Primary (citable) accession number: O70473 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2003 |
Last sequence update: | February 13, 2019 | |
Last modified: | May 25, 2022 | |
This is version 87 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families