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Protein

Docking protein 2

Gene

Dok2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.

GO - Molecular functioni

  • transmembrane receptor protein tyrosine kinase adaptor activity Source: MGI

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-210993 Tie2 Signaling
R-MMU-8853659 RET signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Docking protein 2
Alternative name(s):
Dok-related protein
Short name:
Dok-R
Downstream of tyrosine kinase 2
IL-four receptor-interacting protein
Short name:
FRIP
p56(dok-2)
Gene namesi
Name:Dok2
Synonyms:Frip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1332623 Dok2

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi276Y → F: No loss of binding to SH2 domain of RASGAP or NCK. Complete loss of binding to SH2 domain of RASGAP; when associated with F-304. 1 Publication1
Mutagenesisi304Y → F: No loss of binding to SH2 domain of RASGAP. Complete loss of binding to SH2 domain of RASGAP; when associated with F-276. 1 Publication1
Mutagenesisi351Y → F: No loss of binding to SH2 domain of RASGAP. Complete loss of binding to SH2 domain of NCK. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872711 – 412Docking protein 2Add BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276Phosphotyrosine1 Publication1
Modified residuei304Phosphotyrosine1 Publication1
Modified residuei351Phosphotyrosine1 Publication1

Post-translational modificationi

On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-351 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-276 and Tyr-304 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO70469
PaxDbiO70469
PeptideAtlasiO70469
PRIDEiO70469

PTM databases

iPTMnetiO70469
PhosphoSitePlusiO70469

Expressioni

Tissue specificityi

Highly expressed in spleen and lung.2 Publications

Developmental stagei

During embryonic liver development, expressed in the islands of cells, consistent with an expression in hematopoietic precursors.1 Publication

Gene expression databases

BgeeiENSMUSG00000022102 Expressed in 139 organ(s), highest expression level in thymus
CleanExiMM_DOK2
ExpressionAtlasiO70469 baseline and differential
GenevisibleiO70469 MM

Interactioni

Subunit structurei

Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated).3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199268, 6 interactors
IntActiO70469, 10 interactors
MINTiO70469
STRINGi10090.ENSMUSP00000022698

Structurei

3D structure databases

ProteinModelPortaliO70469
SMRiO70469
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 117PHPROSITE-ProRule annotationAdd BLAST111
Domaini149 – 254IRS-type PTBPROSITE-ProRule annotationAdd BLAST106

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi252 – 307Pro-richAdd BLAST56
Compositional biasi326 – 369Pro-richAdd BLAST44

Domaini

PTB domain mediates receptor interaction.

Sequence similaritiesi

Belongs to the DOK family. Type A subfamily.Curated

Phylogenomic databases

eggNOGiKOG4047 Eukaryota
ENOG410XS2S LUCA
GeneTreeiENSGT00730000110348
HOGENOMiHOG000112245
HOVERGENiHBG018962
InParanoidiO70469
KOiK20234
OMAiEYAVPFD
OrthoDBiEOG091G075T
PhylomeDBiO70469
TreeFamiTF324994

Family and domain databases

CDDicd01203 PTB_DOK1_DOK2_DOK3, 1 hit
Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR037751 Dok1/2/3_PTB
IPR002404 IRS_PTB
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PfamiView protein in Pfam
PF02174 IRS, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00310 PTBI, 1 hit
PROSITEiView protein in PROSITE
PS51064 IRS_PTB, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

O70469-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVRMEEPAVK QGFLHLQQQQ TFGKKWRRFA AVLYGESGCA LARLELQDVP
60 70 80 90 100
EKTRRGEATR KVVRLSDCLR VAEVGSEASS PRDTSAFILE TKERLYLLAA
110 120 130 140 150
PSAERSDWIQ AICLLAFPGQ RKGSPGLEEK SGSPCMEENE LYSSSTTGLC
160 170 180 190 200
KEYMVTIRPT EASERCRLRG SYTLRTGVSA LELWGGPEPG TQLYDWPYRF
210 220 230 240 250
LRRFGRDKAT FSFEAGRRCL SGEGNFEFET RHGNEIFQAL EKVIAVQKNA
260 270 280 290 300
TPSGPPSLPA TGPMMPTVLP RPESPYSRPH DSLPSPSPGT LVPGMRPGAP
310 320 330 340 350
EGEYAVPFDT VAHSLRKSFR GLLTGPPPHL PDPLYDSIQE DPGAPLPDHI
360 370 380 390 400
YDEPEGVAAL SLYDRTQRPS GETWREQATA DGGPSSLQQD SSVPDWPQAT
410
EYDNVILKKG PK
Length:412
Mass (Da):45,522
Last modified:August 1, 1998 - v1
Checksum:i02AC02530DBED053
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245A → T in AAH04590 (PubMed:15489334).Curated1
Sequence conflicti330L → F in AAC13266 (PubMed:9478921).Curated1
Sequence conflicti347P → T in AAC13266 (PubMed:9478921).Curated1
Sequence conflicti412Missing in AAH04590 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030627 mRNA Translation: AAC31315.1
AF035117 mRNA Translation: AAC13266.1
AF059583 mRNA Translation: AAC78606.1
BC004590 mRNA Translation: AAH04590.1
CCDSiCCDS27262.1
RefSeqiNP_034201.1, NM_010071.2
UniGeneiMm.243323

Genome annotation databases

EnsembliENSMUST00000022698; ENSMUSP00000022698; ENSMUSG00000022102
GeneIDi13449
KEGGimmu:13449
UCSCiuc007uoy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030627 mRNA Translation: AAC31315.1
AF035117 mRNA Translation: AAC13266.1
AF059583 mRNA Translation: AAC78606.1
BC004590 mRNA Translation: AAH04590.1
CCDSiCCDS27262.1
RefSeqiNP_034201.1, NM_010071.2
UniGeneiMm.243323

3D structure databases

ProteinModelPortaliO70469
SMRiO70469
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199268, 6 interactors
IntActiO70469, 10 interactors
MINTiO70469
STRINGi10090.ENSMUSP00000022698

PTM databases

iPTMnetiO70469
PhosphoSitePlusiO70469

Proteomic databases

EPDiO70469
PaxDbiO70469
PeptideAtlasiO70469
PRIDEiO70469

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022698; ENSMUSP00000022698; ENSMUSG00000022102
GeneIDi13449
KEGGimmu:13449
UCSCiuc007uoy.1 mouse

Organism-specific databases

CTDi9046
MGIiMGI:1332623 Dok2

Phylogenomic databases

eggNOGiKOG4047 Eukaryota
ENOG410XS2S LUCA
GeneTreeiENSGT00730000110348
HOGENOMiHOG000112245
HOVERGENiHBG018962
InParanoidiO70469
KOiK20234
OMAiEYAVPFD
OrthoDBiEOG091G075T
PhylomeDBiO70469
TreeFamiTF324994

Enzyme and pathway databases

ReactomeiR-MMU-210993 Tie2 Signaling
R-MMU-8853659 RET signaling

Miscellaneous databases

PROiPR:O70469
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022102 Expressed in 139 organ(s), highest expression level in thymus
CleanExiMM_DOK2
ExpressionAtlasiO70469 baseline and differential
GenevisibleiO70469 MM

Family and domain databases

CDDicd01203 PTB_DOK1_DOK2_DOK3, 1 hit
Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR037751 Dok1/2/3_PTB
IPR002404 IRS_PTB
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PfamiView protein in Pfam
PF02174 IRS, 1 hit
PF00169 PH, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00310 PTBI, 1 hit
PROSITEiView protein in PROSITE
PS51064 IRS_PTB, 1 hit
PS50003 PH_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDOK2_MOUSE
AccessioniPrimary (citable) accession number: O70469
Secondary accession number(s): O70272, Q99KL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: August 1, 1998
Last modified: September 12, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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