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Protein

Myosin-binding protein C, cardiac-type

Gene

Mybpc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi206ZincBy similarity1
Metal bindingi208ZincBy similarity1
Metal bindingi221ZincBy similarity1
Metal bindingi223ZincBy similarity1

GO - Molecular functioni

  • actin filament binding Source: GO_Central
  • identical protein binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • muscle alpha-actinin binding Source: GO_Central
  • myosin binding Source: MGI
  • myosin heavy chain binding Source: MGI
  • structural constituent of cytoskeleton Source: MGI
  • structural constituent of muscle Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Muscle protein
Biological processCell adhesion
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-binding protein C, cardiac-type
Short name:
Cardiac MyBP-C
Alternative name(s):
C-protein, cardiac muscle isoform
Gene namesi
Name:Mybpc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:102844 Mybpc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726941 – 1270Myosin-binding protein C, cardiac-typeAdd BLAST1270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei47PhosphoserineCombined sources1
Modified residuei72PhosphoserineCombined sources1
Modified residuei273Phosphoserine; by PKA and PKC1 Publication1
Modified residuei282Phosphoserine; by PKA and PKC1 Publication1
Modified residuei302Phosphoserine; by PKA and PKC1 Publication1
Modified residuei307PhosphoserineCombined sources1
Modified residuei423PhosphoserineCombined sources1
Disulfide bondi432 ↔ 439PROSITE-ProRule annotation
Modified residuei455PhosphoserineCombined sources1
Modified residuei546PhosphoserineCombined sources1
Modified residuei603PhosphothreonineBy similarity1
Modified residuei1237Omega-N-methylarginineCombined sources1

Post-translational modificationi

Substrate for phosphorylation by PKA and PKC. Reversible phosphorylation appears to modulate contraction (By similarity).By similarity
Polyubiquitinated.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO70468
PaxDbiO70468
PeptideAtlasiO70468
PRIDEiO70468

PTM databases

iPTMnetiO70468
PhosphoSitePlusiO70468

Expressioni

Gene expression databases

CleanExiMM_MYBPC3

Interactioni

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-48624N
IntActiO70468, 35 interactors
MINTiO70468
STRINGi10090.ENSMUSP00000127070

Structurei

Secondary structure

11270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO70468
SMRiO70468
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini151 – 254Ig-like C2-type 1Add BLAST104
Domaini358 – 448Ig-like C2-type 2Add BLAST91
Domaini449 – 539Ig-like C2-type 3Add BLAST91
Domaini540 – 629Ig-like C2-type 4Add BLAST90
Domaini641 – 767Ig-like C2-type 5Add BLAST127
Domaini770 – 866Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST97
Domaini868 – 963Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96
Domaini967 – 1061Ig-like C2-type 6Add BLAST95
Domaini1064 – 1159Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST96
Domaini1177 – 1270Ig-like C2-type 7Add BLAST94

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi100 – 150Pro-richAdd BLAST51

Sequence similaritiesi

Belongs to the immunoglobulin superfamily. MyBP family.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat

Phylogenomic databases

eggNOGiENOG410IFCI Eukaryota
ENOG4110AYI LUCA
HOVERGENiHBG052560
InParanoidiO70468

Family and domain databases

CDDicd00063 FN3, 3 hits
Gene3Di2.60.40.10, 11 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
PfamiView protein in Pfam
PF00041 fn3, 3 hits
PF07679 I-set, 8 hits
SMARTiView protein in SMART
SM00060 FN3, 3 hits
SM00409 IG, 8 hits
SM00408 IGc2, 6 hits
SUPFAMiSSF48726 SSF48726, 8 hits
SSF49265 SSF49265, 2 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 3 hits
PS50835 IG_LIKE, 7 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O70468-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPEPGKKPVS AFNKKPRSAE VTAGSAAVFE AETERSGVMV RWQRDGSDIT
60 70 80 90 100
ANDKYGLAAE GKRHTLTVRD ASPDDQGSYA VIAGSSKVKF DLKVTEPAPP
110 120 130 140 150
EKAESEVAPG APEEVPAPAT ELEESVSSPE GSVSVTQDGS AAEHQGAPDD
160 170 180 190 200
PIGLFLMRPQ DGEVTVGGSI VFSARVAGAS LLKPPVVKWF KGKWVDLSSK
210 220 230 240 250
VGQHLQLHDS YDRASKVYLF ELHITDAQTT SAGGYRCEVS TKDKFDSCNF
260 270 280 290 300
NLTVHEAIGS GDLDLRSAFR RTSLAGAGRR TSDSHEDAGT PDFSSLLKKR
310 320 330 340 350
DSFRRDSKLE APAEEDVWEI LRQAPPSEYE RIAFQHGVEA CHRPLKRLKG
360 370 380 390 400
MKQDEKKSTA FQKKLEPAYQ VNKGHKIRLT VELADPDAEV KWLKNGQEIQ
410 420 430 440 450
MSGSKYIFES VGAKRTLTIS QCSLADDAAY QCVVGGEKCS TELFVKEPPV
460 470 480 490 500
LITRSLEDQL VMVGQRVEFE CEVSEEGAQV KWLKDGVELT REETFKYRFK
510 520 530 540 550
KDGRKHHLII NEATLEDAGH YAVRTSGGQS LAELIVQEKK LEVYQSIADL
560 570 580 590 600
AVGAKDQAVF KCEVSDENVR GVWLKNGKEL VPDNRIKVSH IGRVHKLTID
610 620 630 640 650
DVTPADEADY SFVPEGFACN LSAKLHFMEV KIDFVPRQEP PKIHLDCPGS
660 670 680 690 700
TPDTIVVVTG NKLRLDVPIS GDPAPTVVWQ KTVTQGKKAS AGPHPDAPED
710 720 730 740 750
AGADEEWVFD KKLLCETEGR VRVETTKDRS VFTVEGAEKE DEGVYTVTVK
760 770 780 790 800
NPVGEDQVNL TVKVIDVPDA PAAPKISNVG EDSCTVQWEP PAYDGGQPVL
810 820 830 840 850
GYILERKKKK SYRWMRLNFD LLRELSHEAR RMIEGVAYEM RVYAVNAVGM
860 870 880 890 900
SRPSPASQPF MPIGPPGEPT HLAVEDVSDT TVSLKWRPPE RVGAGGLDGY
910 920 930 940 950
SVEYCQEGCS EWTPALQGLT ERRSMLVKDL PTGARLLFRV RAHNVAGPGG
960 970 980 990 1000
PIVTKEPVTV QEILQRPRLQ LPRHLRQTIQ KKVGEPVNLL IPFQGKPRPQ
1010 1020 1030 1040 1050
VTWTKEGQPL AGEEVSIRNS PTDTILFIRA ARRTHSGTYQ VTVRIENMED
1060 1070 1080 1090 1100
KATLILQIVD KPSPPQDIRI VETWGFNVAL EWKPPQDDGN TEIWGYTVQK
1110 1120 1130 1140 1150
ADKKTMEWFT VLEHYRRTHC VVSELIIGNG YYFRVFSHNM VGSSDKAAAT
1160 1170 1180 1190 1200
KEPVFIPRPG ITYEPPKYKA LDFSEAPSFT QPLANRSIIA GYNAILCCAV
1210 1220 1230 1240 1250
RGSPKPKISW FKNGLDLGED ARFRMFCKQG VLTLEIRKPC PYDGGVYVCR
1260 1270
ATNLQGEAQC ECRLEVRVPQ
Length:1,270
Mass (Da):140,632
Last modified:August 1, 1998 - v1
Checksum:i699947C3C9B58931
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UIK0Q3UIK0_MOUSE
Myosin-binding protein C, cardiac-t...
Mybpc3
1,278Annotation score:
E9Q9T8E9Q9T8_MOUSE
Myosin-binding protein C, cardiac-t...
Mybpc3
1,277Annotation score:
Q3TF37Q3TF37_MOUSE
Myosin-binding protein C, cardiac-t...
Mybpc3
1,113Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32E → G in AAC64202 (Ref. 2) Curated1
Sequence conflicti39M → K in AAC64202 (Ref. 2) Curated1
Sequence conflicti113E → K in AAC64202 (Ref. 2) Curated1
Sequence conflicti249N → S in AAC64202 (Ref. 2) Curated1
Sequence conflicti291P → L in AAC64202 (Ref. 2) Curated1
Sequence conflicti339 – 344EACHRP → TDLRGM in AAC64202 (Ref. 2) Curated6
Sequence conflicti659T → A in AAC64202 (Ref. 2) Curated1
Sequence conflicti691A → T in AAC64202 (Ref. 2) Curated1
Sequence conflicti738E → G in AAC64202 (Ref. 2) Curated1
Sequence conflicti923R → T in AAC64202 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059576 mRNA Translation: AAC14570.1
AF097333 mRNA Translation: AAC64202.1
UniGeneiMm.10728

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059576 mRNA Translation: AAC14570.1
AF097333 mRNA Translation: AAC64202.1
UniGeneiMm.10728

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EDQX-ray1.64A/B149-269[»]
ProteinModelPortaliO70468
SMRiO70468
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48624N
IntActiO70468, 35 interactors
MINTiO70468
STRINGi10090.ENSMUSP00000127070

PTM databases

iPTMnetiO70468
PhosphoSitePlusiO70468

Proteomic databases

MaxQBiO70468
PaxDbiO70468
PeptideAtlasiO70468
PRIDEiO70468

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:102844 Mybpc3

Phylogenomic databases

eggNOGiENOG410IFCI Eukaryota
ENOG4110AYI LUCA
HOVERGENiHBG052560
InParanoidiO70468

Miscellaneous databases

ChiTaRSiMybpc3 mouse
PROiPR:O70468
SOURCEiSearch...

Gene expression databases

CleanExiMM_MYBPC3

Family and domain databases

CDDicd00063 FN3, 3 hits
Gene3Di2.60.40.10, 11 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
PfamiView protein in Pfam
PF00041 fn3, 3 hits
PF07679 I-set, 8 hits
SMARTiView protein in SMART
SM00060 FN3, 3 hits
SM00409 IG, 8 hits
SM00408 IGc2, 6 hits
SUPFAMiSSF48726 SSF48726, 8 hits
SSF49265 SSF49265, 2 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 3 hits
PS50835 IG_LIKE, 7 hits
ProtoNetiSearch...

Entry informationi

Entry nameiMYPC3_MOUSE
AccessioniPrimary (citable) accession number: O70468
Secondary accession number(s): O88997
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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