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Protein

14-3-3 protein sigma

Gene

Sfn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.1 Publication

Miscellaneous

14-3-3 proteins have been shown to be PKC activators, but this effect could be non-specific and only due to the acidic nature of the protein.

GO - Molecular functioni

  • identical protein binding Source: MGI
  • phosphoprotein binding Source: UniProtKB
  • protein domain specific binding Source: InterPro
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-111447 Activation of BAD and translocation to mitochondria
R-MMU-1445148 Translocation of SLC2A4 (GLUT4) to the plasma membrane
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-MMU-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein sigma
Alternative name(s):
Stratifin
Gene namesi
Name:Sfn
Synonyms:Mkrn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1891831 Sfn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1909483

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586441 – 24814-3-3 protein sigmaAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineBy similarity1
Modified residuei74PhosphoserineBy similarity1
Modified residuei248PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO70456
MaxQBiO70456
PaxDbiO70456
PeptideAtlasiO70456
PRIDEiO70456

PTM databases

iPTMnetiO70456
PhosphoSitePlusiO70456

Expressioni

Tissue specificityi

Expressed in the basal layer of skin epithelium and in outer root sheath of hair follicle.1 Publication

Inductioni

Induced in damaged or stressed epidermis.1 Publication

Gene expression databases

BgeeiENSMUSG00000047281 Expressed in 215 organ(s), highest expression level in skin of back
CleanExiMM_MKRN3
MM_SFN
GenevisibleiO70456 MM

Interactioni

Subunit structurei

Homodimer. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29 and VPS35. Interacts with GAB2 (By similarity). Interacts with KRT17. Interacts with SAMSN1. Interacts with SRPK2 (By similarity). Interacts with COPS6 (By similarity). Interacts with COP1; this interaction leads to proteasomal degradation (By similarity).Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB (By similarity). Interacts with SLITRK1 (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Interaction with phosphoserine on interacting proteinBy similarity1
Sitei129Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
Krt17Q9QWL73EBI-1544118,EBI-309015

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207743, 7 interactors
IntActiO70456, 5 interactors
MINTiO70456
STRINGi10090.ENSMUSP00000050374

Structurei

3D structure databases

ProteinModelPortaliO70456
SMRiO70456
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
HOGENOMiHOG000240379
HOVERGENiHBG050423
InParanoidiO70456
KOiK06644
OMAiSIEQKGN
OrthoDBiEOG091G0VKY
TreeFamiTF102003

Family and domain databases

CDDicd10019 14-3-3_sigma, 1 hit
Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
IPR037435 14-3-3_sigma
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequencei

Sequence statusi: Complete.

O70456-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERASLIQKA KLAEQAERYE DMAAFMKSAV EKGEELSCEE RNLLSVAYKN
60 70 80 90 100
VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VKEYREKVET ELRGVCDTVL
110 120 130 140 150
GLLDSHLIKG AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA
160 170 180 190 200
YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE
210 220 230 240
AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADSAGEEGG EAPEEPQS
Length:248
Mass (Da):27,706
Last modified:June 28, 2011 - v2
Checksum:iC1C40905824B3F48
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti244 – 248EEPQS → DDPHI in AAC14344 (Ref. 1) Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058798 mRNA Translation: AAC14344.1
AK146490 mRNA Translation: BAE27209.1
AK169358 mRNA Translation: BAE41107.1
AL627228 Genomic DNA Translation: CAM14836.1
CH466552 Genomic DNA Translation: EDL30056.1
CCDSiCCDS18754.1
RefSeqiNP_061224.2, NM_018754.2
UniGeneiMm.44482

Genome annotation databases

EnsembliENSMUST00000057311; ENSMUSP00000050374; ENSMUSG00000047281
GeneIDi55948
KEGGimmu:55948
UCSCiuc008vdc.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058798 mRNA Translation: AAC14344.1
AK146490 mRNA Translation: BAE27209.1
AK169358 mRNA Translation: BAE41107.1
AL627228 Genomic DNA Translation: CAM14836.1
CH466552 Genomic DNA Translation: EDL30056.1
CCDSiCCDS18754.1
RefSeqiNP_061224.2, NM_018754.2
UniGeneiMm.44482

3D structure databases

ProteinModelPortaliO70456
SMRiO70456
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207743, 7 interactors
IntActiO70456, 5 interactors
MINTiO70456
STRINGi10090.ENSMUSP00000050374

Chemistry databases

ChEMBLiCHEMBL1909483

PTM databases

iPTMnetiO70456
PhosphoSitePlusiO70456

Proteomic databases

EPDiO70456
MaxQBiO70456
PaxDbiO70456
PeptideAtlasiO70456
PRIDEiO70456

Protocols and materials databases

DNASUi55948
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057311; ENSMUSP00000050374; ENSMUSG00000047281
GeneIDi55948
KEGGimmu:55948
UCSCiuc008vdc.1 mouse

Organism-specific databases

CTDi2810
MGIiMGI:1891831 Sfn

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
HOGENOMiHOG000240379
HOVERGENiHBG050423
InParanoidiO70456
KOiK06644
OMAiSIEQKGN
OrthoDBiEOG091G0VKY
TreeFamiTF102003

Enzyme and pathway databases

ReactomeiR-MMU-111447 Activation of BAD and translocation to mitochondria
R-MMU-1445148 Translocation of SLC2A4 (GLUT4) to the plasma membrane
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-MMU-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Miscellaneous databases

PROiPR:O70456
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047281 Expressed in 215 organ(s), highest expression level in skin of back
CleanExiMM_MKRN3
MM_SFN
GenevisibleiO70456 MM

Family and domain databases

CDDicd10019 14-3-3_sigma, 1 hit
Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
IPR037435 14-3-3_sigma
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei1433S_MOUSE
AccessioniPrimary (citable) accession number: O70456
Secondary accession number(s): Q3TEZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 28, 2011
Last modified: September 12, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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