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Protein

BRCA1-associated RING domain protein 1

Gene

Bard1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri44 – 81RING-typePROSITE-ProRule annotationAdd BLAST38

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5689603 UCH proteinases
R-MMU-5689901 Metalloprotease DUBs
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
BRCA1-associated RING domain protein 1 (EC:2.3.2.27By similarity)
Short name:
BARD-1
Alternative name(s):
RING-type E3 ubiquitin transferase BARD1Curated
Gene namesi
Name:Bard1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1328361 Bard1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558201 – 765BRCA1-associated RING domain protein 1Add BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki152Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei378PhosphoserineBy similarity1
Modified residuei381PhosphothreonineBy similarity1
Cross-linki411Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO70445
MaxQBiO70445
PaxDbiO70445
PeptideAtlasiO70445
PRIDEiO70445

PTM databases

iPTMnetiO70445
PhosphoSitePlusiO70445

Expressioni

Gene expression databases

BgeeiENSMUSG00000026196 Expressed in 79 organ(s), highest expression level in secondary oocyte
CleanExiMM_BARD1
GenevisibleiO70445 MM

Interactioni

Subunit structurei

Homo- and heterodimer. Heterodimer (RING-type zinc finger) with BRCA1. Heterodimer (via ANK repeats and BRCT domains) with CSTF1/CSTF-50. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Interacts with UBXN1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198301, 4 interactors
ComplexPortaliCPX-972 BRCC ubiquitin ligase complex
IntActiO70445, 4 interactors
STRINGi10090.ENSMUSP00000027393

Structurei

3D structure databases

ProteinModelPortaliO70445
SMRiO70445
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati415 – 447ANK 1Add BLAST33
Repeati448 – 480ANK 2Add BLAST33
Repeati481 – 513ANK 3Add BLAST33
Repeati514 – 534ANK 4; degenerateAdd BLAST21
Domaini549 – 641BRCT 1PROSITE-ProRule annotationAdd BLAST93
Domaini655 – 765BRCT 2PROSITE-ProRule annotationAdd BLAST111

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 113Required for BRCA1 bindingBy similarityAdd BLAST94
Regioni542 – 546Flexible linkerBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi530 – 533Poly-Leu4

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri44 – 81RING-typePROSITE-ProRule annotationAdd BLAST38

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
KOG4362 Eukaryota
ENOG410Z0YV LUCA
GeneTreeiENSGT00760000119090
HOGENOMiHOG000237306
HOVERGENiHBG050662
InParanoidiO70445
KOiK10683
OMAiCYFYLGG
OrthoDBiEOG091G0JNZ
PhylomeDBiO70445
TreeFamiTF326440

Family and domain databases

CDDicd00204 ANK, 1 hit
cd00027 BRCT, 2 hits
cd16496 RING-HC_BARD1, 1 hit
Gene3Di1.25.40.20, 1 hit
3.30.40.10, 1 hit
3.40.50.10190, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR033097 BARD1
IPR039503 BARD1_Znf-RING
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR24171:SF8 PTHR24171:SF8, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF14835 zf-RING_6, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SM00292 BRCT, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF52113 SSF52113, 2 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits
PS50172 BRCT, 2 hits
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

O70445-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPRRPPRVCS GNQPAPVPAM EPATDGLWAH SRAALARLEK LLRCSRCANI
60 70 80 90 100
LKEPVCLGGC EHIFCSGCIS DCVGSGCPVC YTPAWILDLK INRQLDSMIQ
110 120 130 140 150
LSSKLQNLLH DNKDSKDNTS RASLFGDAER KKNSIKMWFS PRSKKVRYVV
160 170 180 190 200
TKVSVQTQPQ KAKDDKAQEA SMYEFVSATP PVAVPKSAKT ASRTSAKKHP
210 220 230 240 250
KKSVAKINRE ENLRPETKDS RFDSKEELKE EKVVSCSQIP VMERPRVNGE
260 270 280 290 300
IDLLASGSVV EPECSGSLTE VSLPLAEHIV SPDTVSKNEE TPEKKVCVKD
310 320 330 340 350
LRSGGSNGNR KGCHRPTTST SDSCGSNIPS TSRGIGEPAL LAENVVLVDC
360 370 380 390 400
SSLPSGQLQV DVTLRRKSNA SDDPLSLSPG TPPPLLNNST HRQMMSSPST
410 420 430 440 450
VKLSSGMPAR KRNHRGETLL HIASIKGDIP SVEYLLQNGN DPNVKDHAGW
460 470 480 490 500
TPLHEACSHG HLKVVELLLQ HNALVNTPGY QNDSPLHDAV KSGHIDIVKV
510 520 530 540 550
LLSHGASRNA VNIFGVRPVD YTDNENIRSL LLLPEENESF STSQCSIVNT
560 570 580 590 600
GQRKNGPLVF IGSGLSSQQQ KMLSKLETVL KAKKCMEFDS TVTHVIVPDE
610 620 630 640 650
EAQSTLKCML GILSGCWILK FDWVKACLDS KVREQEEKYE VPGGPQRSRL
660 670 680 690 700
NREQLLPKLF DGCYFFLGGN FKHHPRDDLL KLIAAAGGKV LSRKPKPDSD
710 720 730 740 750
VTQTINTVAY HAKPESDQRF CTQYIVYEDL FNCHPERVRQ GKVWMAPSTW
760
LISCIMAFEL LPLDS
Length:765
Mass (Da):84,254
Last modified:August 1, 1998 - v1
Checksum:iC43DD0625F2F1AE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057157 mRNA Translation: AAC18095.1
BC128370 mRNA Translation: AAI28371.1
BC128371 mRNA Translation: AAI28372.1
CCDSiCCDS15029.1
RefSeqiNP_031551.1, NM_007525.3
UniGeneiMm.10764
Mm.490738

Genome annotation databases

EnsembliENSMUST00000027393; ENSMUSP00000027393; ENSMUSG00000026196
GeneIDi12021
KEGGimmu:12021
UCSCiuc007bjm.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057157 mRNA Translation: AAC18095.1
BC128370 mRNA Translation: AAI28371.1
BC128371 mRNA Translation: AAI28372.1
CCDSiCCDS15029.1
RefSeqiNP_031551.1, NM_007525.3
UniGeneiMm.10764
Mm.490738

3D structure databases

ProteinModelPortaliO70445
SMRiO70445
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198301, 4 interactors
ComplexPortaliCPX-972 BRCC ubiquitin ligase complex
IntActiO70445, 4 interactors
STRINGi10090.ENSMUSP00000027393

PTM databases

iPTMnetiO70445
PhosphoSitePlusiO70445

Proteomic databases

EPDiO70445
MaxQBiO70445
PaxDbiO70445
PeptideAtlasiO70445
PRIDEiO70445

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027393; ENSMUSP00000027393; ENSMUSG00000026196
GeneIDi12021
KEGGimmu:12021
UCSCiuc007bjm.2 mouse

Organism-specific databases

CTDi580
MGIiMGI:1328361 Bard1

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
KOG4362 Eukaryota
ENOG410Z0YV LUCA
GeneTreeiENSGT00760000119090
HOGENOMiHOG000237306
HOVERGENiHBG050662
InParanoidiO70445
KOiK10683
OMAiCYFYLGG
OrthoDBiEOG091G0JNZ
PhylomeDBiO70445
TreeFamiTF326440

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5689603 UCH proteinases
R-MMU-5689901 Metalloprotease DUBs
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Miscellaneous databases

ChiTaRSiBard1 mouse
PROiPR:O70445
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026196 Expressed in 79 organ(s), highest expression level in secondary oocyte
CleanExiMM_BARD1
GenevisibleiO70445 MM

Family and domain databases

CDDicd00204 ANK, 1 hit
cd00027 BRCT, 2 hits
cd16496 RING-HC_BARD1, 1 hit
Gene3Di1.25.40.20, 1 hit
3.30.40.10, 1 hit
3.40.50.10190, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR033097 BARD1
IPR039503 BARD1_Znf-RING
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR24171:SF8 PTHR24171:SF8, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF14835 zf-RING_6, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SM00292 BRCT, 2 hits
SUPFAMiSSF48403 SSF48403, 1 hit
SSF52113 SSF52113, 2 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits
PS50172 BRCT, 2 hits
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBARD1_MOUSE
AccessioniPrimary (citable) accession number: O70445
Secondary accession number(s): A2VCQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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