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Protein

Four and a half LIM domains protein 2

Gene

Fhl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation (By similarity). Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PubMed:22851699).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C4-typeSequence analysisAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Four and a half LIM domains protein 2
Short name:
FHL-2
Alternative name(s):
Skeletal muscle LIM-protein 3
Short name:
SLIM-3
Gene namesi
Name:Fhl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1338762 Fhl2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757381 – 279Four and a half LIM domains protein 2Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki78Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki167Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki220Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei238PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO70433
PaxDbiO70433
PeptideAtlasiO70433
PRIDEiO70433

PTM databases

iPTMnetiO70433
PhosphoSitePlusiO70433

Expressioni

Tissue specificityi

Highly expressed in heart but also detectable in brain and skeletal muscle.1 Publication

Inductioni

Up-regulated in hearts exposed to isoproterenol (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000008136 Expressed in 267 organ(s), highest expression level in heart
CleanExiMM_FHL2
ExpressionAtlasiO70433 baseline and differential
GenevisibleiO70433 MM

Interactioni

Subunit structurei

Interacts with ZNF638 and TTN/titin. Interacts with E4F1. Interacts with GRB7. Interacts with SIRT1 and FOXO1. Interacts with CEFIP (By similarity). Interacts with calcineurin (PubMed:22851699).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Plcg2Q8CIH53EBI-299379,EBI-617954

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199669, 7 interactors
IntActiO70433, 6 interactors
MINTiO70433
STRINGi10090.ENSMUSP00000008280

Structurei

3D structure databases

ProteinModelPortaliO70433
SMRiO70433
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 92LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST53
Domaini101 – 153LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST53
Domaini162 – 212LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST51
Domaini221 – 275LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST55

Domaini

The third LIM zinc-binding mediates interaction with E4F1.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 31C4-typeSequence analysisAdd BLAST25

Keywords - Domaini

LIM domain, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410INGK Eukaryota
ENOG410XRFY LUCA
GeneTreeiENSGT00760000118910
HOGENOMiHOG000231075
HOVERGENiHBG074526
InParanoidiO70433
KOiK14380
OMAiVACFEAL
OrthoDBiEOG091G07C5
PhylomeDBiO70433
TreeFamiTF321684

Family and domain databases

InterProiView protein in InterPro
IPR037987 FHL2/3/5
IPR001781 Znf_LIM
PANTHERiPTHR24205 PTHR24205, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 4 hits
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O70433-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTERFDCHHC NESLYGKKYI LKEENPHCVA CFEELYANTC EECGTPIGCD
60 70 80 90 100
CKDLSYKDRH WHEGCFHCSR CGSSLVDKPF AAKEEQLLCT DCYSNEYSSK
110 120 130 140 150
CQECKKTIMP GTRKMEYKGS SWHETCFTCQ RCQQPIGTKS FIPKENQNFC
160 170 180 190 200
VPCYEKQYAL QCVQCKKPIT TGGVTYREQP WHKECFVCTA CKKQLSGQRF
210 220 230 240 250
TARDEFPYCL TCFCDLYAKK CAGCTNPISG LGGTKYISFE ERQWHNDCFN
260 270
CKKCSLSLVG RGFLTERDDI LCPDCGKDI
Length:279
Mass (Da):32,073
Last modified:August 1, 1998 - v1
Checksum:i6D8CBC4B4424BFF2
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WR06A0A087WR06_MOUSE
Four and a half LIM domains protein...
Fhl2
21Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055889 mRNA Translation: AAC12770.1
U77040 mRNA Translation: AAB19211.2
AF114381 mRNA Translation: AAD53230.1
AF153340 mRNA Translation: AAD34170.1
CCDSiCCDS14922.1
RefSeqiNP_001276462.1, NM_001289533.1
NP_034342.1, NM_010212.4
XP_011236736.1, XM_011238434.2
UniGeneiMm.6799

Genome annotation databases

EnsembliENSMUST00000008280; ENSMUSP00000008280; ENSMUSG00000008136
ENSMUST00000185893; ENSMUSP00000141170; ENSMUSG00000008136
GeneIDi14200
KEGGimmu:14200
UCSCiuc007avk.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055889 mRNA Translation: AAC12770.1
U77040 mRNA Translation: AAB19211.2
AF114381 mRNA Translation: AAD53230.1
AF153340 mRNA Translation: AAD34170.1
CCDSiCCDS14922.1
RefSeqiNP_001276462.1, NM_001289533.1
NP_034342.1, NM_010212.4
XP_011236736.1, XM_011238434.2
UniGeneiMm.6799

3D structure databases

ProteinModelPortaliO70433
SMRiO70433
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199669, 7 interactors
IntActiO70433, 6 interactors
MINTiO70433
STRINGi10090.ENSMUSP00000008280

PTM databases

iPTMnetiO70433
PhosphoSitePlusiO70433

Proteomic databases

EPDiO70433
PaxDbiO70433
PeptideAtlasiO70433
PRIDEiO70433

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008280; ENSMUSP00000008280; ENSMUSG00000008136
ENSMUST00000185893; ENSMUSP00000141170; ENSMUSG00000008136
GeneIDi14200
KEGGimmu:14200
UCSCiuc007avk.2 mouse

Organism-specific databases

CTDi2274
MGIiMGI:1338762 Fhl2

Phylogenomic databases

eggNOGiENOG410INGK Eukaryota
ENOG410XRFY LUCA
GeneTreeiENSGT00760000118910
HOGENOMiHOG000231075
HOVERGENiHBG074526
InParanoidiO70433
KOiK14380
OMAiVACFEAL
OrthoDBiEOG091G07C5
PhylomeDBiO70433
TreeFamiTF321684

Miscellaneous databases

ChiTaRSiFhl2 mouse
PROiPR:O70433
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000008136 Expressed in 267 organ(s), highest expression level in heart
CleanExiMM_FHL2
ExpressionAtlasiO70433 baseline and differential
GenevisibleiO70433 MM

Family and domain databases

InterProiView protein in InterPro
IPR037987 FHL2/3/5
IPR001781 Znf_LIM
PANTHERiPTHR24205 PTHR24205, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 4 hits
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiFHL2_MOUSE
AccessioniPrimary (citable) accession number: O70433
Secondary accession number(s): P97448
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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