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Protein

Membrane primary amine oxidase

Gene

Aoc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis (By similarity).By similarity

Catalytic activityi

RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei386Proton acceptorBy similarity1
Active sitei471Schiff-base intermediate with substrate; via topaquinoneBy similarity1
Metal bindingi520Copper; via tele nitrogenBy similarity1
Metal bindingi522Copper; via tele nitrogenBy similarity1
Metal bindingi529Calcium 1By similarity1
Metal bindingi530Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi531Calcium 1By similarity1
Metal bindingi572Calcium 2By similarity1
Metal bindingi663Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi667Calcium 2By similarity1
Metal bindingi673Calcium 1By similarity1
Metal bindingi674Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi684Copper; via pros nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCell adhesion
LigandCalcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-211945 Phase I - Functionalization of compounds

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane primary amine oxidase (EC:1.4.3.21By similarity)
Alternative name(s):
Copper amine oxidase
Semicarbazide-sensitive amine oxidase
Short name:
SSAO
Vascular adhesion protein 1
Short name:
VAP-1
Gene namesi
Name:Aoc3
Synonyms:Vap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1306797 Aoc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 765ExtracellularSequence analysisAdd BLAST738

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4727

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000641031 – 765Membrane primary amine oxidaseAdd BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi137N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi198 ↔ 199By similarity
Glycosylationi212O-linked (GalNAc...) threonineBy similarity1
Glycosylationi232N-linked (GlcNAc...) asparagineBy similarity1
Glycosylationi294N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi404 ↔ 430By similarity
Modified residuei4712',4',5'-topaquinoneBy similarity1
Glycosylationi592N-linked (GlcNAc...) (complex) asparagineBy similarity1
Glycosylationi659N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi666N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi734 ↔ 741By similarity
Disulfide bondi748InterchainBy similarity

Post-translational modificationi

N- and O-glycosylated.By similarity
Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, TPQ

Proteomic databases

MaxQBiO70423
PaxDbiO70423
PeptideAtlasiO70423
PRIDEiO70423

PTM databases

iPTMnetiO70423
PhosphoSitePlusiO70423

Expressioni

Gene expression databases

BgeeiENSMUSG00000019326 Expressed in 159 organ(s), highest expression level in epididymal fat pad
CleanExiMM_AOC3
ExpressionAtlasiO70423 baseline and differential
GenevisibleiO70423 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Forms a heterodimer with AOC2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198116, 1 interactor
IntActiO70423, 4 interactors
MINTiO70423
STRINGi10090.ENSMUSP00000099394

Chemistry databases

BindingDBiO70423

Structurei

3D structure databases

ProteinModelPortaliO70423
SMRiO70423
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni384 – 394Substrate bindingBy similarityAdd BLAST11
Regioni468 – 473Substrate bindingBy similarity6
Regioni578 – 585Heparin-bindingBy similarity8

Sequence similaritiesi

Belongs to the copper/topaquinone oxidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1186 Eukaryota
COG3733 LUCA
GeneTreeiENSGT00510000046461
HOGENOMiHOG000233919
HOVERGENiHBG004164
InParanoidiO70423
KOiK00276
OMAiISWGRYQ
OrthoDBiEOG091G02WY
PhylomeDBiO70423
TreeFamiTF314750

Family and domain databases

Gene3Di2.70.98.20, 1 hit
InterProiView protein in InterPro
IPR000269 Cu_amine_oxidase
IPR015798 Cu_amine_oxidase_C
IPR036460 Cu_amine_oxidase_C_sf
IPR016182 Cu_amine_oxidase_N-reg
IPR015800 Cu_amine_oxidase_N2
IPR015802 Cu_amine_oxidase_N3
PANTHERiPTHR10638 PTHR10638, 1 hit
PfamiView protein in Pfam
PF01179 Cu_amine_oxid, 1 hit
PF02727 Cu_amine_oxidN2, 1 hit
PF02728 Cu_amine_oxidN3, 1 hit
PRINTSiPR00766 CUDAOXIDASE
SUPFAMiSSF49998 SSF49998, 1 hit
SSF54416 SSF54416, 2 hits
PROSITEiView protein in PROSITE
PS01164 COPPER_AMINE_OXID_1, 1 hit
PS01165 COPPER_AMINE_OXID_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O70423-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTQKTTLVLL ALAVITIFAL VCVLLAGRSG DGGGLSQPLH CPSVLPSVQP
60 70 80 90 100
RTHPSQSQPF ADLSPEELTA VMSFLTKHLG PGLVDAAQAR PSDNCVFSVE
110 120 130 140 150
LQLPAKAAAL AHLDRGGPPP VREALAIIFF GGQPKPNVSE LVVGPLPHPS
160 170 180 190 200
YMRDVTVERH GGPLPYYRRP VLDREYQDIE EMIFHRELPQ ASGLLHHCCF
210 220 230 240 250
YKHQGQNLLT MTTAPRGLQS GDRATWFGLY YNLSGAGFYP HPIGLELLID
260 270 280 290 300
HKALDPALWT IQKVFYQGRY YESLTQLEDQ FEAGLVNVVL VPNNGTGGSW
310 320 330 340 350
SLKSSVPPGP APPLQFHPQG PRFSVQGSQV SSSLWAFSFG LGAFSGPRIF
360 370 380 390 400
DIRFQGERVA YEISVQEAIA LYGGNSPASM STCYVDGSFG IGKYSTPLIR
410 420 430 440 450
GVDCPYLATY VDWHFLLESQ APKTLRDAFC VFEQNQGLPL RRHHSDFYSH
460 470 480 490 500
YFGGVVGTVL VVRSVSTLLN YDYIWDMVFH PNGAIEVKFH ATGYISSAFF
510 520 530 540 550
FGAGEKFGNR VGAHTLGTVH THSAHFKVDL DVAGLKNWAW AEDMAFVPTI
560 570 580 590 600
VPWQPEYQMQ RLQVTRKLLE TEEEAAFPLG GATPRYLYLA SNHSNKWGHR
610 620 630 640 650
RGYRIQILSF AGKPLPQESP IEKAFTWGRY HLAVTQRKEE EPSSSSIFNQ
660 670 680 690 700
NDPWTPTVNF TDFISNETIA GEDLVAWVTA GFLHIPHAED IPNTVTAGNS
710 720 730 740 750
VGFFLRPYNF FDEDPSFHSA DSIYFREGQD ATACEVNPLA CLSQTATCAP
760
EIPAFSHGGF AYRDN
Length:765
Mass (Da):84,534
Last modified:January 23, 2007 - v3
Checksum:i7489ED67D3DBB44D
GO
Isoform 2 (identifier: O70423-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     630-765: Missing.

Note: No experimental confirmation available.
Show »
Length:629
Mass (Da):69,527
Checksum:i96121FDE3D7473F4
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A4I8A2A4I8_MOUSE
Amine oxidase
Aoc3
470Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti659N → D in AAD09199 (PubMed:10092636).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016604630 – 765Missing in isoform 2. 1 PublicationAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054831 mRNA Translation: AAC23747.1
AF078705 Genomic DNA Translation: AAC35839.1
AF115411 mRNA Translation: AAD09199.1
AL590969 Genomic DNA Translation: CAM19551.1
BC080857 mRNA Translation: AAH80857.1
CCDSiCCDS25465.1 [O70423-1]
RefSeqiNP_033805.1, NM_009675.2 [O70423-1]
UniGeneiMm.67281

Genome annotation databases

EnsembliENSMUST00000103105; ENSMUSP00000099394; ENSMUSG00000019326 [O70423-1]
GeneIDi11754
KEGGimmu:11754
UCSCiuc007lop.2 mouse [O70423-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054831 mRNA Translation: AAC23747.1
AF078705 Genomic DNA Translation: AAC35839.1
AF115411 mRNA Translation: AAD09199.1
AL590969 Genomic DNA Translation: CAM19551.1
BC080857 mRNA Translation: AAH80857.1
CCDSiCCDS25465.1 [O70423-1]
RefSeqiNP_033805.1, NM_009675.2 [O70423-1]
UniGeneiMm.67281

3D structure databases

ProteinModelPortaliO70423
SMRiO70423
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198116, 1 interactor
IntActiO70423, 4 interactors
MINTiO70423
STRINGi10090.ENSMUSP00000099394

Chemistry databases

BindingDBiO70423
ChEMBLiCHEMBL4727

PTM databases

iPTMnetiO70423
PhosphoSitePlusiO70423

Proteomic databases

MaxQBiO70423
PaxDbiO70423
PeptideAtlasiO70423
PRIDEiO70423

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103105; ENSMUSP00000099394; ENSMUSG00000019326 [O70423-1]
GeneIDi11754
KEGGimmu:11754
UCSCiuc007lop.2 mouse [O70423-1]

Organism-specific databases

CTDi8639
MGIiMGI:1306797 Aoc3

Phylogenomic databases

eggNOGiKOG1186 Eukaryota
COG3733 LUCA
GeneTreeiENSGT00510000046461
HOGENOMiHOG000233919
HOVERGENiHBG004164
InParanoidiO70423
KOiK00276
OMAiISWGRYQ
OrthoDBiEOG091G02WY
PhylomeDBiO70423
TreeFamiTF314750

Enzyme and pathway databases

ReactomeiR-MMU-211945 Phase I - Functionalization of compounds

Miscellaneous databases

PROiPR:O70423
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019326 Expressed in 159 organ(s), highest expression level in epididymal fat pad
CleanExiMM_AOC3
ExpressionAtlasiO70423 baseline and differential
GenevisibleiO70423 MM

Family and domain databases

Gene3Di2.70.98.20, 1 hit
InterProiView protein in InterPro
IPR000269 Cu_amine_oxidase
IPR015798 Cu_amine_oxidase_C
IPR036460 Cu_amine_oxidase_C_sf
IPR016182 Cu_amine_oxidase_N-reg
IPR015800 Cu_amine_oxidase_N2
IPR015802 Cu_amine_oxidase_N3
PANTHERiPTHR10638 PTHR10638, 1 hit
PfamiView protein in Pfam
PF01179 Cu_amine_oxid, 1 hit
PF02727 Cu_amine_oxidN2, 1 hit
PF02728 Cu_amine_oxidN3, 1 hit
PRINTSiPR00766 CUDAOXIDASE
SUPFAMiSSF49998 SSF49998, 1 hit
SSF54416 SSF54416, 2 hits
PROSITEiView protein in PROSITE
PS01164 COPPER_AMINE_OXID_1, 1 hit
PS01165 COPPER_AMINE_OXID_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAOC3_MOUSE
AccessioniPrimary (citable) accession number: O70423
Secondary accession number(s): A2A4I7, Q66JM4, Q9R055
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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