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Protein

Vesicle-associated membrane protein 8

Gene

Vamp8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (By similarity). Also required for dense-granule secretion in platelets (By similarity). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (PubMed:15363411). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 81 Publication
Short name:
VAMP-81 Publication
Alternative name(s):
Endobrevin1 Publication
Short name:
EdbBy similarity
Gene namesi
Name:Vamp8Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1336882 Vamp8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 75CytoplasmicSequence analysisAdd BLAST75
Transmembranei76 – 96Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini97 – 101VesicularSequence analysis5

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24K → A: Blocks internalization from cell surface; when associated with A-27. 1 Publication1
Mutagenesisi27M → A: Blocks internalization from cell surface; when associated with A-24. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067371 – 101Vesicle-associated membrane protein 8Add BLAST101

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei18PhosphoserineBy similarity1
Modified residuei28PhosphothreonineBy similarity1
Modified residuei48PhosphothreonineBy similarity1
Modified residuei54PhosphothreonineCombined sources1
Modified residuei55PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO70404
MaxQBiO70404
PaxDbiO70404
PeptideAtlasiO70404
PRIDEiO70404

PTM databases

iPTMnetiO70404
PhosphoSitePlusiO70404

Expressioni

Tissue specificityi

Expressed abundantly in the kidney, less in the liver, brain, kidney, heart, lung, pancreas and placenta.2 Publications

Gene expression databases

CleanExiMM_VAMP8

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity). Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:15363411). Interacts with CALM (PubMed:22118466). SNARE complex formation and binding by CALM are mutually exclusive processes for VAMP8 (PubMed:22118466).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Interaction with STX8By similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
PicalmO550124EBI-1812572,EBI-915601From Rattus norvegicus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204497, 2 interactors
CORUMiO70404
IntActiO70404, 4 interactors
MINTiO70404
STRINGi10090.ENSMUSP00000059501

Structurei

Secondary structure

1101
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO70404
SMRiO70404
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 72v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiO70404
KOiK08512
PhylomeDBiO70404
TreeFamiTF320419

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS50892 V_SNARE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O70404-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEASGSAGN DRVRNLQSEV EGVKNIMTQN VERILSRGEN LDHLRNKTED
60 70 80 90 100
LEATSEHFKT TSQKVARKFW WKNVKMIVII CVIVLIIVIL IILFATGTIP

T
Length:101
Mass (Da):11,451
Last modified:August 1, 1998 - v1
Checksum:i0A2FDB6B69E06C3E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J0R1A0A0R4J0R1_MOUSE
Vesicle-associated membrane protein...
Vamp8 mCG_129303
101Annotation score:
A0A0U1RPE8A0A0U1RPE8_MOUSE
Vesicle-associated membrane protein...
Vamp8
75Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36S → A (PubMed:16141072).Curated1
Sequence conflicti36S → A (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053724 mRNA Translation: AAC06371.1
AF045661 mRNA Translation: AAC23665.1
AF247556 Genomic DNA Translation: AAK48423.1
AK002268 mRNA Translation: BAB21977.1
AK013279 mRNA Translation: BAB28766.1
BC012668 mRNA Translation: AAH12668.1
CCDSiCCDS39514.1
RefSeqiNP_058074.2, NM_016794.3
UniGeneiMm.1838

Genome annotation databases

GeneIDi22320
KEGGimmu:22320
UCSCiuc009cin.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053724 mRNA Translation: AAC06371.1
AF045661 mRNA Translation: AAC23665.1
AF247556 Genomic DNA Translation: AAK48423.1
AK002268 mRNA Translation: BAB21977.1
AK013279 mRNA Translation: BAB28766.1
BC012668 mRNA Translation: AAH12668.1
CCDSiCCDS39514.1
RefSeqiNP_058074.2, NM_016794.3
UniGeneiMm.1838

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZYMX-ray2.03A/B/C11-41[»]
ProteinModelPortaliO70404
SMRiO70404
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204497, 2 interactors
CORUMiO70404
IntActiO70404, 4 interactors
MINTiO70404
STRINGi10090.ENSMUSP00000059501

PTM databases

iPTMnetiO70404
PhosphoSitePlusiO70404

Proteomic databases

EPDiO70404
MaxQBiO70404
PaxDbiO70404
PeptideAtlasiO70404
PRIDEiO70404

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22320
KEGGimmu:22320
UCSCiuc009cin.2 mouse

Organism-specific databases

CTDi8673
MGIiMGI:1336882 Vamp8

Phylogenomic databases

eggNOGiKOG0860 Eukaryota
COG5143 LUCA
HOGENOMiHOG000042711
HOVERGENiHBG006675
InParanoidiO70404
KOiK08512
PhylomeDBiO70404
TreeFamiTF320419

Miscellaneous databases

PROiPR:O70404
SOURCEiSearch...

Gene expression databases

CleanExiMM_VAMP8

Family and domain databases

InterProiView protein in InterPro
IPR001388 Synaptobrevin
IPR016444 Synaptobrevin/VAMP
PfamiView protein in Pfam
PF00957 Synaptobrevin, 1 hit
PIRSFiPIRSF005409 Synaptobrevin_euk, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS50892 V_SNARE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP8_MOUSE
AccessioniPrimary (citable) accession number: O70404
Secondary accession number(s): Q9CRA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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