UniProtKB - O70378 (EMC8_MOUSE)
Protein
ER membrane protein complex subunit 8
Gene
Emc8
Organism
Mus musculus (Mouse)
Status
Functioni
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes.By similarity
Names & Taxonomyi
Protein namesi | Recommended name: ER membrane protein complex subunit 8Alternative name(s): Neighbor of COX4 |
Gene namesi | Name:Emc8 Synonyms:Cox4al, Cox4nb, Noc4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1343095, Emc8 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- ER membrane protein complex Source: UniProtKB
Mitochondrion
- mitochondrion Source: MGI
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000221188 | 1 – 207 | ER membrane protein complex subunit 8Add BLAST | 207 |
Proteomic databases
EPDi | O70378 |
jPOSTi | O70378 |
PaxDbi | O70378 |
PeptideAtlasi | O70378 |
PRIDEi | O70378 |
PTM databases
iPTMneti | O70378 |
PhosphoSitePlusi | O70378 |
SwissPalmi | O70378 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031819, Expressed in epiblast (generic) and 309 other tissues |
ExpressionAtlasi | O70378, baseline and differential |
Genevisiblei | O70378, MM |
Interactioni
Subunit structurei
Component of the ER membrane protein complex (EMC). EMC8 and EMC9 are mutually exclusive subunits of the EMC complex.
By similarityProtein-protein interaction databases
BioGRIDi | 201800, 1 interactor |
ComplexPortali | CPX-5882, Endoplasmic reticulum membrane complex, ECM8 variant |
STRINGi | 10090.ENSMUSP00000034277 |
Miscellaneous databases
RNActi | O70378, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 147 | MPNPROSITE-ProRule annotationAdd BLAST | 144 |
Sequence similaritiesi
Belongs to the EMC8/EMC9 family.Curated
Phylogenomic databases
eggNOGi | KOG3289, Eukaryota |
GeneTreei | ENSGT00390000006738 |
HOGENOMi | CLU_087337_0_1_1 |
InParanoidi | O70378 |
OMAi | MQIDAYA |
OrthoDBi | 1284861at2759 |
PhylomeDBi | O70378 |
TreeFami | TF313860 |
Family and domain databases
CDDi | cd08060, MPN_UPF0172, 1 hit |
InterProi | View protein in InterPro IPR005366, EMC8/9 IPR037518, MPN |
PANTHERi | PTHR12941, PTHR12941, 1 hit |
Pfami | View protein in Pfam PF03665, UPF0172, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
O70378-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPGVKLTTQA YCKMVLHGAK YPHCAVNGLL VAERQRPRKE HPPGAGSHTL
60 70 80 90 100
FVDCIPLFHG TLALTPMLEV ALTLIDSWCK DNSYVIAGYY QANERVKDAS
110 120 130 140 150
PNQVAEKVAS RIAEGFGDAA LIMVDNAKFT MDCAAPTIHV YEQHENRWRC
160 170 180 190 200
RDPHHDYCED WPEAQRISAS LLDSRSYETL VDFDNHLDDI RSDWTNPEIN
KAVLHLC
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0QWY0 | M0QWY0_MOUSE | ER membrane protein complex subunit... | Emc8 | 128 | Annotation score: | ||
M0QWI7 | M0QWI7_MOUSE | ER membrane protein complex subunit... | Emc8 | 114 | Annotation score: | ||
M0QWS0 | M0QWS0_MOUSE | ER membrane protein complex subunit... | Emc8 | 77 | Annotation score: | ||
M0QWP2 | M0QWP2_MOUSE | ER membrane protein complex subunit... | Emc8 | 143 | Annotation score: | ||
M0QWC9 | M0QWC9_MOUSE | ER membrane protein complex subunit... | Emc8 | 167 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF052621 mRNA Translation: AAC12933.1 BC009103 mRNA Translation: AAH09103.1 |
CCDSi | CCDS22719.1 |
RefSeqi | NP_035056.1, NM_010926.5 |
Genome annotation databases
Ensembli | ENSMUST00000034277; ENSMUSP00000034277; ENSMUSG00000031819 |
GeneIDi | 18117 |
KEGGi | mmu:18117 |
UCSCi | uc009nrf.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF052621 mRNA Translation: AAC12933.1 BC009103 mRNA Translation: AAH09103.1 |
CCDSi | CCDS22719.1 |
RefSeqi | NP_035056.1, NM_010926.5 |
3D structure databases
SMRi | O70378 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201800, 1 interactor |
ComplexPortali | CPX-5882, Endoplasmic reticulum membrane complex, ECM8 variant |
STRINGi | 10090.ENSMUSP00000034277 |
PTM databases
iPTMneti | O70378 |
PhosphoSitePlusi | O70378 |
SwissPalmi | O70378 |
Proteomic databases
EPDi | O70378 |
jPOSTi | O70378 |
PaxDbi | O70378 |
PeptideAtlasi | O70378 |
PRIDEi | O70378 |
Protocols and materials databases
Antibodypediai | 30647, 250 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034277; ENSMUSP00000034277; ENSMUSG00000031819 |
GeneIDi | 18117 |
KEGGi | mmu:18117 |
UCSCi | uc009nrf.1, mouse |
Organism-specific databases
CTDi | 10328 |
MGIi | MGI:1343095, Emc8 |
Phylogenomic databases
eggNOGi | KOG3289, Eukaryota |
GeneTreei | ENSGT00390000006738 |
HOGENOMi | CLU_087337_0_1_1 |
InParanoidi | O70378 |
OMAi | MQIDAYA |
OrthoDBi | 1284861at2759 |
PhylomeDBi | O70378 |
TreeFami | TF313860 |
Miscellaneous databases
BioGRID-ORCSi | 18117, 5 hits in 17 CRISPR screens |
ChiTaRSi | Emc8, mouse |
PROi | PR:O70378 |
RNActi | O70378, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031819, Expressed in epiblast (generic) and 309 other tissues |
ExpressionAtlasi | O70378, baseline and differential |
Genevisiblei | O70378, MM |
Family and domain databases
CDDi | cd08060, MPN_UPF0172, 1 hit |
InterProi | View protein in InterPro IPR005366, EMC8/9 IPR037518, MPN |
PANTHERi | PTHR12941, PTHR12941, 1 hit |
Pfami | View protein in Pfam PF03665, UPF0172, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EMC8_MOUSE | |
Accessioni | O70378Primary (citable) accession number: O70378 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families