UniProtKB - O70370 (CATS_MOUSE)
Protein
Cathepsin S
Gene
Ctss
Organism
Mus musculus (Mouse)
Status
Functioni
Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.By similarity
Catalytic activityi
- Similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound. EC:3.4.22.27
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 147 | By similarity | 1 | |
Active sitei | 287 | By similarity | 1 | |
Active sitei | 307 | By similarity | 1 |
GO - Molecular functioni
- collagen binding Source: MGI
- cysteine-type endopeptidase activity Source: Reactome
- cysteine-type peptidase activity Source: MGI
- fibronectin binding Source: MGI
- laminin binding Source: MGI
- peptidase activity Source: MGI
- proteoglycan binding Source: MGI
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: UniProtKB
- antigen processing and presentation of peptide antigen Source: MGI
- basement membrane disassembly Source: BHF-UCL
- bone resorption Source: MGI
- collagen catabolic process Source: MGI
- immune response Source: GO_Central
- positive regulation of cation channel activity Source: CACAO
- positive regulation of inflammatory response Source: MGI
- protein processing Source: MGI
- proteolysis Source: MGI
- proteolysis involved in cellular protein catabolic process Source: BHF-UCL
- regulation of sensory perception of pain Source: MGI
- response to acidic pH Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Enzyme and pathway databases
BRENDAi | 3.4.22.27, 3474 |
Reactomei | R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1679131, Trafficking and processing of endosomal TLR R-MMU-2022090, Assembly of collagen fibrils and other multimeric structures R-MMU-2132295, MHC class II antigen presentation R-MMU-6798695, Neutrophil degranulation |
Protein family/group databases
MEROPSi | C01.034 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ctss Synonyms:Cats |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107341, Ctss |
Subcellular locationi
Endosome
- early endosome lumen Source: Reactome
- late endosome Source: MGI
Extracellular region or secreted
- extracellular space Source: UniProtKB
Lysosome
- lysosome Source: MGI
Other locations
- cell surface Source: MGI
- intracellular membrane-bounded organelle Source: MGI
- membrane Source: MGI
- phagocytic vesicle Source: UniProtKB
Keywords - Cellular componenti
Cytoplasmic vesicle, Lysosome, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | Sequence analysisAdd BLAST | 17 | |
PropeptideiPRO_0000026315 | 18 – 122 | Activation peptideSequence analysisAdd BLAST | 105 | |
ChainiPRO_0000026316 | 123 – 340 | Cathepsin SAdd BLAST | 218 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 120 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 134 ↔ 233 | By similarity | ||
Disulfide bondi | 144 ↔ 189 | By similarity | ||
Disulfide bondi | 178 ↔ 222 | By similarity | ||
Disulfide bondi | 281 ↔ 329 | By similarity |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
CPTACi | non-CPTAC-3897 |
EPDi | O70370 |
MaxQBi | O70370 |
PaxDbi | O70370 |
PRIDEi | O70370 |
PTM databases
GlyGeni | O70370, 1 site |
iPTMneti | O70370 |
PhosphoSitePlusi | O70370 |
SwissPalmi | O70370 |
Expressioni
Tissue specificityi
Widely expressed with highest expression found in non-skeletal tissues. Relatively high levels found in skeletal tissues. Expressed in spleen, B cells, dendritic cells and macrophages (PubMed:9545226).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000038642, Expressed in camera-type eye and 295 other tissues |
Genevisiblei | O70370, MM |
Interactioni
GO - Molecular functioni
- fibronectin binding Source: MGI
- laminin binding Source: MGI
- proteoglycan binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198976, 2 interactors |
STRINGi | 10090.ENSMUSP00000015667 |
Chemistry databases
BindingDBi | O70370 |
Miscellaneous databases
RNActi | O70370, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O70370 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000155176 |
HOGENOMi | CLU_012184_1_2_1 |
InParanoidi | O70370 |
OMAi | PYKAMDE |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O70370-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRAPGHAAIR WLFWMPLVCS VAMEQLQRDP TLDYHWDLWK KTHEKEYKDK
60 70 80 90 100
NEEEVRRLIW EKNLKFIMIH NLEYSMGMHT YQVGMNDMGD MTNEEILCRM
110 120 130 140 150
GALRIPRQSP KTVTFRSYSN RTLPDTVDWR EKGCVTEVKY QGSCGACWAF
160 170 180 190 200
SAVGALEGQL KLKTGKLISL SAQNLVDCSN EEKYGNKGCG GGYMTEAFQY
210 220 230 240 250
IIDNGGIEAD ASYPYKATDE KCHYNSKNRA ATCSRYIQLP FGDEDALKEA
260 270 280 290 300
VATKGPVSVG IDASHSSFFF YKSGVYDDPS CTGNVNHGVL VVGYGTLDGK
310 320 330 340
DYWLVKNSWG LNFGDQGYIR MARNNKNHCG IASYCSYPEI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF6WR04 | F6WR04_MOUSE | Cathepsin S | Ctss | 341 | Annotation score: |
Sequence cautioni
The sequence CAA05360 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 34 | Y → H in CAA05360 (Ref. 2) Curated | 1 | |
Sequence conflicti | 97 | L → S in AAB94925 (Ref. 1) Curated | 1 | |
Sequence conflicti | 97 | L → S in AAC05781 (Ref. 1) Curated | 1 | |
Sequence conflicti | 106 | P → S in AAB94925 (Ref. 1) Curated | 1 | |
Sequence conflicti | 106 | P → S in AAC05781 (Ref. 1) Curated | 1 | |
Sequence conflicti | 146 | A → S in CAA77184 (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 218 | T → M in AAB94925 (Ref. 1) Curated | 1 | |
Sequence conflicti | 218 | T → M in CAA05360 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF051732 , AF051727, AF051728, AF051729, AF051726, AF051730, AF051731 Genomic DNA Translation: AAC05781.1 AF038546 mRNA Translation: AAB94925.1 AJ002386 mRNA Translation: CAA05360.1 Frameshift. AC092203 Genomic DNA No translation available. Y18466 mRNA Translation: CAA77184.1 AJ223208 mRNA Translation: CAA11182.1 |
CCDSi | CCDS50992.1 |
RefSeqi | NP_067256.4, NM_021281.3 |
Genome annotation databases
Ensembli | ENSMUST00000116304; ENSMUSP00000112006; ENSMUSG00000038642 |
GeneIDi | 13040 |
KEGGi | mmu:13040 |
UCSCi | uc008qjz.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF051732 , AF051727, AF051728, AF051729, AF051726, AF051730, AF051731 Genomic DNA Translation: AAC05781.1 AF038546 mRNA Translation: AAB94925.1 AJ002386 mRNA Translation: CAA05360.1 Frameshift. AC092203 Genomic DNA No translation available. Y18466 mRNA Translation: CAA77184.1 AJ223208 mRNA Translation: CAA11182.1 |
CCDSi | CCDS50992.1 |
RefSeqi | NP_067256.4, NM_021281.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1M0H | model | - | A | 1-340 | [»] | |
4BPV | X-ray | 2.00 | A/B/C/D/E/K | 116-340 | [»] | |
4BQV | X-ray | 1.70 | A/B/C/D/E/F/G/H | 116-340 | [»] | |
4BS5 | X-ray | 1.25 | A | 116-340 | [»] | |
4BS6 | X-ray | 1.20 | A/B | 116-340 | [»] | |
4BSQ | X-ray | 1.96 | A | 116-340 | [»] | |
4MZO | X-ray | 1.47 | A/B/C/D/E/F/G/H | 116-340 | [»] | |
4MZS | X-ray | 1.85 | A/B | 116-340 | [»] | |
SMRi | O70370 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198976, 2 interactors |
STRINGi | 10090.ENSMUSP00000015667 |
Chemistry databases
BindingDBi | O70370 |
ChEMBLi | CHEMBL4098 |
GuidetoPHARMACOLOGYi | 2353 |
Protein family/group databases
MEROPSi | C01.034 |
PTM databases
GlyGeni | O70370, 1 site |
iPTMneti | O70370 |
PhosphoSitePlusi | O70370 |
SwissPalmi | O70370 |
Proteomic databases
CPTACi | non-CPTAC-3897 |
EPDi | O70370 |
MaxQBi | O70370 |
PaxDbi | O70370 |
PRIDEi | O70370 |
Protocols and materials databases
Antibodypediai | 849, 458 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000116304; ENSMUSP00000112006; ENSMUSG00000038642 |
GeneIDi | 13040 |
KEGGi | mmu:13040 |
UCSCi | uc008qjz.3, mouse |
Organism-specific databases
CTDi | 1520 |
MGIi | MGI:107341, Ctss |
Phylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000155176 |
HOGENOMi | CLU_012184_1_2_1 |
InParanoidi | O70370 |
OMAi | PYKAMDE |
Enzyme and pathway databases
BRENDAi | 3.4.22.27, 3474 |
Reactomei | R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1679131, Trafficking and processing of endosomal TLR R-MMU-2022090, Assembly of collagen fibrils and other multimeric structures R-MMU-2132295, MHC class II antigen presentation R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 13040, 2 hits in 13 CRISPR screens |
ChiTaRSi | Ctss, mouse |
PROi | PR:O70370 |
RNActi | O70370, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038642, Expressed in camera-type eye and 295 other tissues |
Genevisiblei | O70370, MM |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CATS_MOUSE | |
Accessioni | O70370Primary (citable) accession number: O70370 Secondary accession number(s): E9QK18, O54973 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families