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Protein

Cathepsin S

Gene

Ctss

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.

Catalytic activityi

Similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei147By similarity1
Active sitei287By similarity1
Active sitei307By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.27 3474
ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-2132295 MHC class II antigen presentation
R-MMU-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiC01.034

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin S (EC:3.4.22.27)
Gene namesi
Name:Ctss
Synonyms:Cats
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:107341 Ctss

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4098
GuidetoPHARMACOLOGYi2353

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000002631518 – 122Activation peptideSequence analysisAdd BLAST105
ChainiPRO_0000026316123 – 340Cathepsin SAdd BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi134 ↔ 233By similarity
Disulfide bondi144 ↔ 189By similarity
Disulfide bondi178 ↔ 222By similarity
Disulfide bondi281 ↔ 329By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiO70370
MaxQBiO70370
PaxDbiO70370
PRIDEiO70370

PTM databases

iPTMnetiO70370
PhosphoSitePlusiO70370

Expressioni

Tissue specificityi

Widely expressed with highest expression found in non-skeletal tissues. Relatively high levels found in skeletal tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000038642 Expressed in 277 organ(s), highest expression level in camera-type eye
CleanExiMM_CTSS
ExpressionAtlasiO70370 baseline and differential
GenevisibleiO70370 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112006

Chemistry databases

BindingDBiO70370

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO70370
SMRiO70370
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140823
HOVERGENiHBG011513
InParanoidiO70370
KOiK01368
OMAiYYDPSCT

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O70370-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRAPGHAAIR WLFWMPLVCS VAMEQLQRDP TLDYHWDLWK KTHEKEYKDK
60 70 80 90 100
NEEEVRRLIW EKNLKFIMIH NLEYSMGMHT YQVGMNDMGD MTNEEILCRM
110 120 130 140 150
GALRIPRQSP KTVTFRSYSN RTLPDTVDWR EKGCVTEVKY QGSCGACWAF
160 170 180 190 200
SAVGALEGQL KLKTGKLISL SAQNLVDCSN EEKYGNKGCG GGYMTEAFQY
210 220 230 240 250
IIDNGGIEAD ASYPYKATDE KCHYNSKNRA ATCSRYIQLP FGDEDALKEA
260 270 280 290 300
VATKGPVSVG IDASHSSFFF YKSGVYDDPS CTGNVNHGVL VVGYGTLDGK
310 320 330 340
DYWLVKNSWG LNFGDQGYIR MARNNKNHCG IASYCSYPEI
Length:340
Mass (Da):38,475
Last modified:July 27, 2011 - v2
Checksum:i10550C5106318C47
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6WR04F6WR04_MOUSE
Cathepsin S
Ctss
341Annotation score:

Sequence cautioni

The sequence CAA05360 differs from that shown. Reason: Frameshift at position 30.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34Y → H in CAA05360 (Ref. 2) Curated1
Sequence conflicti97L → S in AAB94925 (Ref. 1) Curated1
Sequence conflicti97L → S in AAC05781 (Ref. 1) Curated1
Sequence conflicti106P → S in AAB94925 (Ref. 1) Curated1
Sequence conflicti106P → S in AAC05781 (Ref. 1) Curated1
Sequence conflicti146A → S in CAA77184 (PubMed:19468303).Curated1
Sequence conflicti218T → M in AAB94925 (Ref. 1) Curated1
Sequence conflicti218T → M in CAA05360 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051732
, AF051727, AF051728, AF051729, AF051726, AF051730, AF051731 Genomic DNA Translation: AAC05781.1
AF038546 mRNA Translation: AAB94925.1
AJ002386 mRNA Translation: CAA05360.1 Frameshift.
AC092203 Genomic DNA No translation available.
Y18466 mRNA Translation: CAA77184.1
AJ223208 mRNA Translation: CAA11182.1
CCDSiCCDS50992.1
RefSeqiNP_067256.4, NM_021281.3
UniGeneiMm.3619

Genome annotation databases

EnsembliENSMUST00000116304; ENSMUSP00000112006; ENSMUSG00000038642
GeneIDi13040
KEGGimmu:13040
UCSCiuc008qjz.3 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051732
, AF051727, AF051728, AF051729, AF051726, AF051730, AF051731 Genomic DNA Translation: AAC05781.1
AF038546 mRNA Translation: AAB94925.1
AJ002386 mRNA Translation: CAA05360.1 Frameshift.
AC092203 Genomic DNA No translation available.
Y18466 mRNA Translation: CAA77184.1
AJ223208 mRNA Translation: CAA11182.1
CCDSiCCDS50992.1
RefSeqiNP_067256.4, NM_021281.3
UniGeneiMm.3619

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M0Hmodel-A1-340[»]
4BPVX-ray2.00A/B/C/D/E/K116-340[»]
4BQVX-ray1.70A/B/C/D/E/F/G/H116-340[»]
4BS5X-ray1.25A116-340[»]
4BS6X-ray1.20A/B116-340[»]
4BSQX-ray1.96A116-340[»]
4MZOX-ray1.47A/B/C/D/E/F/G/H116-340[»]
4MZSX-ray1.85A/B116-340[»]
ProteinModelPortaliO70370
SMRiO70370
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112006

Chemistry databases

BindingDBiO70370
ChEMBLiCHEMBL4098
GuidetoPHARMACOLOGYi2353

Protein family/group databases

MEROPSiC01.034

PTM databases

iPTMnetiO70370
PhosphoSitePlusiO70370

Proteomic databases

EPDiO70370
MaxQBiO70370
PaxDbiO70370
PRIDEiO70370

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000116304; ENSMUSP00000112006; ENSMUSG00000038642
GeneIDi13040
KEGGimmu:13040
UCSCiuc008qjz.3 mouse

Organism-specific databases

CTDi1520
MGIiMGI:107341 Ctss

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140823
HOVERGENiHBG011513
InParanoidiO70370
KOiK01368
OMAiYYDPSCT

Enzyme and pathway databases

BRENDAi3.4.22.27 3474
ReactomeiR-MMU-1474228 Degradation of the extracellular matrix
R-MMU-1679131 Trafficking and processing of endosomal TLR
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-2132295 MHC class II antigen presentation
R-MMU-6798695 Neutrophil degranulation

Miscellaneous databases

ChiTaRSiCtss mouse
PROiPR:O70370
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038642 Expressed in 277 organ(s), highest expression level in camera-type eye
CleanExiMM_CTSS
ExpressionAtlasiO70370 baseline and differential
GenevisibleiO70370 MM

Family and domain databases

CDDicd02248 Peptidase_C1A, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR039417 Peptidase_C1A_papain-like
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCATS_MOUSE
AccessioniPrimary (citable) accession number: O70370
Secondary accession number(s): E9QK18, O54973
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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