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Protein

Decapping and exoribonuclease protein

Gene

Dxo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ribonuclease that specifically degrades pre-mRNAs with a defective 5' end cap and is part of a pre-mRNA capping quality control. Has decapping, pyrophosphohydrolase and 5'-3' exonuclease activities. Has decapping activity toward incomplete 5' end cap mRNAs such as unmethylated 5' end-capped RNA to release GpppN and 5' end monophosphate RNA. The 5' end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5' end triphosphate to release pyrophosphates.1 Publication

Caution

In contrast to canonical decapping enzymes DCP2 and NUDT16, which release m7pppG (m7GDP), the decapping activity releases the entire cap structure GpppN and a 5' end monophosphate RNA.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 2 magnesium ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58Substrate1
Binding sitei101Substrate1
Binding sitei132Substrate1
Metal bindingi192Magnesium 11 Publication1
Metal bindingi192Magnesium 21 Publication1
Binding sitei217Substrate1
Metal bindingi234Magnesium 21 Publication1
Binding sitei234Substrate1
Metal bindingi236Magnesium 11 Publication1
Metal bindingi236Magnesium 21 Publication1
Metal bindingi253Magnesium 11 Publication1
Metal bindingi254Magnesium 1; via carbonyl oxygen1 Publication1
Binding sitei280Substrate1

GO - Molecular functioni

GO - Biological processi

  • mRNA catabolic process Source: UniProtKB
  • nuclear mRNA surveillance Source: UniProtKB
  • nucleic acid phosphodiester bond hydrolysis Source: UniProtKB
  • RNA destabilization Source: UniProtKB

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease, RNA-binding
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Decapping and exoribonuclease protein (EC:3.1.13.-, EC:3.6.1.-)
Short name:
DXO
Alternative name(s):
Dom-3 homolog Z
Gene namesi
Name:Dxo
Synonyms:Dom3z, Ng6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1890444 Dxo

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi234E → A: Abolishes the decapping activity. 1 Publication1
Mutagenesisi236D → A: Abolishes the decapping activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498231 – 397Decapping and exoribonuclease proteinAdd BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei392PhosphothreonineBy similarity1
Modified residuei394PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO70348
MaxQBiO70348
PaxDbiO70348
PRIDEiO70348

PTM databases

iPTMnetiO70348
PhosphoSitePlusiO70348

Expressioni

Gene expression databases

CleanExiMM_DOM3Z

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047018

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO70348
SMRiO70348
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO70348

Family & Domainsi

Sequence similaritiesi

Belongs to the DXO/Dom3Z family.Curated

Phylogenomic databases

eggNOGiKOG1982 Eukaryota
ENOG410ZAFR LUCA
HOGENOMiHOG000200647
HOVERGENiHBG080627
InParanoidiO70348
KOiK14845
PhylomeDBiO70348
TreeFamiTF322812

Family and domain databases

InterProiView protein in InterPro
IPR013961 RAI1
IPR039039 RAI1-like_fam
PANTHERiPTHR12395 PTHR12395, 1 hit
PfamiView protein in Pfam
PF08652 RAI1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All

O70348-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPRGTKRKA EKTEVEKPLN KLPRAVPSLR TQPSLYSGPF PFYRRPSELG
60 70 80 90 100
CFSLDAQRQY HGDARALRYY SPPPINGPGP DFDLRDGYPD RYQPRDEEVQ
110 120 130 140 150
ERLDHLLRWV LEHRNQLEGG PGWLAGATVT WRGHLTKLLT TPYERQEGWQ
160 170 180 190 200
LAASRFQGTL YLSEVETPAA RAQRLARPPL LRELMYMGYK FEQYMCADKP
210 220 230 240 250
GGSPDPSGEV NTNVAYCSVL RSRLGNHPLL FSGEVDCLNP QAPCTQPPSC
260 270 280 290 300
YVELKTSKEM HSPGQWRSFY RHKLLKWWAQ SFLPGVPHVV AGFRNPEGFV
310 320 330 340 350
CSLKTFPTME MFENVRNDRE GWNPSVCMNF CAAFLSFAQS TVVQDDPRLV
360 370 380 390
HLFSWEPGGP VTVSVHRDAP YAFLPSWYVE TMTQDLPPLS KTPSPKD
Length:397
Mass (Da):45,280
Last modified:September 19, 2006 - v2
Checksum:i02022DF0A77D503E
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J288A0A0R4J288_MOUSE
DOM-3 homolog Z (C. elegans), isofo...
Dxo Dom3z, mCG_15915
397Annotation score:
G3UX82G3UX82_MOUSE
Decapping and exoribonuclease prote...
Dxo Dom3z
245Annotation score:
G3UZ39G3UZ39_MOUSE
Decapping and exoribonuclease prote...
Dxo Dom3z
304Annotation score:
G3UWP7G3UWP7_MOUSE
Decapping and exoribonuclease prote...
Dxo Dom3z
118Annotation score:
G3UZP8G3UZP8_MOUSE
Decapping and exoribonuclease prote...
Dxo Dom3z
144Annotation score:
G3UWW3G3UWW3_MOUSE
Decapping and exoribonuclease prote...
Dxo Dom3z
74Annotation score:
G3UYP2G3UYP2_MOUSE
Decapping and exoribonuclease prote...
Dxo Dom3z
45Annotation score:
G3UZV8G3UZV8_MOUSE
Decapping and exoribonuclease prote...
Dxo Dom3z
61Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20N → H in AAC05281 (PubMed:14656967).Curated1
Sequence conflicti28S → L in AAC05281 (PubMed:14656967).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049850 Genomic DNA Translation: AAC05281.1
BC004713 mRNA Translation: AAH04713.1
CCDSiCCDS28660.1
RefSeqiNP_001157242.1, NM_001163770.1
NP_291091.2, NM_033613.2
UniGeneiMm.275309

Genome annotation databases

GeneIDi112403
KEGGimmu:112403
UCSCiuc008cdr.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049850 Genomic DNA Translation: AAC05281.1
BC004713 mRNA Translation: AAH04713.1
CCDSiCCDS28660.1
RefSeqiNP_001157242.1, NM_001163770.1
NP_291091.2, NM_033613.2
UniGeneiMm.275309

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FQIX-ray2.01A1-397[»]
3FQJX-ray2.62A1-397[»]
4J7LX-ray1.80A27-384[»]
4J7MX-ray1.70A27-384[»]
4J7NX-ray1.50A27-384[»]
5ULIX-ray2.10A27-384[»]
ProteinModelPortaliO70348
SMRiO70348
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047018

PTM databases

iPTMnetiO70348
PhosphoSitePlusiO70348

Proteomic databases

EPDiO70348
MaxQBiO70348
PaxDbiO70348
PRIDEiO70348

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi112403
KEGGimmu:112403
UCSCiuc008cdr.2 mouse

Organism-specific databases

CTDi1797
MGIiMGI:1890444 Dxo

Phylogenomic databases

eggNOGiKOG1982 Eukaryota
ENOG410ZAFR LUCA
HOGENOMiHOG000200647
HOVERGENiHBG080627
InParanoidiO70348
KOiK14845
PhylomeDBiO70348
TreeFamiTF322812

Miscellaneous databases

EvolutionaryTraceiO70348
PROiPR:O70348
SOURCEiSearch...

Gene expression databases

CleanExiMM_DOM3Z

Family and domain databases

InterProiView protein in InterPro
IPR013961 RAI1
IPR039039 RAI1-like_fam
PANTHERiPTHR12395 PTHR12395, 1 hit
PfamiView protein in Pfam
PF08652 RAI1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDXO_MOUSE
AccessioniPrimary (citable) accession number: O70348
Secondary accession number(s): Q99KD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: July 18, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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