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Protein

Ribonuclease H1

Gene

Rnaseh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.By similarity

Activity regulationi

In the presence of magnesium, manganese is inhibitory.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144Magnesium 1PROSITE-ProRule annotation1
Metal bindingi144Magnesium 2PROSITE-ProRule annotation1
Metal bindingi185Magnesium 1PROSITE-ProRule annotation1
Metal bindingi209Magnesium 1PROSITE-ProRule annotation1
Metal bindingi273Magnesium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • DNA replication, removal of RNA primer Source: GO_Central
  • mitochondrial DNA replication Source: MGI

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H1 (EC:3.1.26.4)
Short name:
RNase H1
Gene namesi
Name:Rnaseh1
Synonyms:Rnh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1335073 Rnaseh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954341 – 285Ribonuclease H1Add BLAST285

Proteomic databases

EPDiO70338
MaxQBiO70338
PaxDbiO70338
PeptideAtlasiO70338
PRIDEiO70338

PTM databases

iPTMnetiO70338
PhosphoSitePlusiO70338

Expressioni

Gene expression databases

CleanExiMM_RNASEH1
MM_RNH1

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020959

Structurei

3D structure databases

ProteinModelPortaliO70338
SMRiO70338
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini135 – 281RNase HPROSITE-ProRule annotationAdd BLAST147

Sequence similaritiesi

Belongs to the RNase H family.Curated

Phylogenomic databases

eggNOGiKOG3752 Eukaryota
COG0328 LUCA
HOGENOMiHOG000040465
HOVERGENiHBG002135
InParanoidiO70338
PhylomeDBiO70338

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.40.970.10, 1 hit
InterProiView protein in InterPro
IPR009027 Ribosomal_L9/RNase_H1_N
IPR017067 RNase_H1_euk
IPR011320 RNase_H1_N
IPR037056 RNase_H1_N_sf
IPR012337 RNaseH-like_sf
IPR002156 RNaseH_domain
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF01693 Cauli_VI, 1 hit
PF00075 RNase_H, 1 hit
PIRSFiPIRSF036852 Ribonuclease_H1_euk, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
SSF55658 SSF55658, 1 hit
PROSITEiView protein in PROSITE
PS50879 RNASE_H, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

O70338-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRWLLPLSRT VTLAVVRLRR GICGLGMFYA VRRGRRTGVF LSWSECKAQV
60 70 80 90 100
DRFPAARFKK FATEDEAWAF VRSSSSPDGS KGQESAHEQK SQAKTSKRPR
110 120 130 140 150
EPLGEGEELP EPGPKHTRQD TEPAAVVSKD TFSYMGESVI VYTDGCCSSN
160 170 180 190 200
GRKRARAGIG VYWGPGHPLN VGIRLPGRQT NQRAEIHAAC KAIMQAKAQN
210 220 230 240 250
ISKLVLYTDS MFTINGITNW VQGWKKNGWR TSTGKDVINK EDFMELDELT
260 270 280
QGMDIQWMHI PGHSGFVGNE EADRLAREGA KQSED
Length:285
Mass (Da):31,805
Last modified:August 1, 1998 - v1
Checksum:i92B09F8EFAFA822F
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QLN8E9QLN8_MOUSE
Ribonuclease H1
Rnaseh1
285Annotation score:
A0A1Y7VM77A0A1Y7VM77_MOUSE
Ribonuclease H1
Rnaseh1
198Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93A → V in AAH19411 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048993 mRNA Translation: AAC78562.1
BC019411 mRNA Translation: AAH19411.1
CCDSiCCDS25852.1
UniGeneiMm.10152

Similar proteinsi

Entry informationi

Entry nameiRNH1_MOUSE
AccessioniPrimary (citable) accession number: O70338
Secondary accession number(s): Q8VCR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: September 12, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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