Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Gremlin-1

Gene

Grem1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop (PubMed:12808456, PubMed:15201225). Down-regulates the BMP4 signaling in a dose-dependent manner (PubMed:15133038). Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (By similarity). Acts as inhibitor of monocyte chemotaxis (By similarity).By similarity3 Publications

GO - Molecular functioni

  • BMP binding Source: UniProtKB
  • cytokine activity Source: UniProtKB-KW
  • heparan sulfate proteoglycan binding Source: BHF-UCL
  • protein homodimerization activity Source: MGI
  • receptor ligand activity Source: BHF-UCL
  • transmembrane receptor protein tyrosine kinase activator activity Source: BHF-UCL
  • vascular endothelial growth factor receptor 2 binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionCytokine

Names & Taxonomyi

Protein namesi
Recommended name:
Gremlin-1
Alternative name(s):
Cysteine knot superfamily 1, BMP antagonist 1
Down-regulated in Mos-transformed cells protein
Gene namesi
Name:Grem1
Synonyms:Cktsf1b1, Drm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1344337 Grem1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24By similarityAdd BLAST24
ChainiPRO_000000671625 – 184Gremlin-1Add BLAST160

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi42N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi94 ↔ 144By similarity
Disulfide bondi108 ↔ 158By similarity
Disulfide bondi118 ↔ 176By similarity
Disulfide bondi122 ↔ 178By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO70326
PeptideAtlasiO70326
PRIDEiO70326

PTM databases

iPTMnetiO70326
PhosphoSitePlusiO70326

Expressioni

Tissue specificityi

Highly expressed in spleen and to a lesser extent in lung, skeletal muscle and kidney. Expressed only in non-transformed cells or primary fibroblasts in culture but not in established transformed or tumor derived cell lines. Broadly expressed in limb bud mesenchyme but restricted to the distal limb bud mesenchyme and concentrated posteriorly. Expressed in ovary especially in granulosa cells of follicles of type 4.3 Publications

Inductioni

Up-regulated by GDF9 dose-dependent manner and BMP4 in granulosa cells. Highly regulated during folliculogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000074934 Expressed in 148 organ(s), highest expression level in fibroblast
CleanExiMM_GREM1
ExpressionAtlasiO70326 baseline and differential
GenevisibleiO70326 MM

Interactioni

Subunit structurei

Homodimer; can also form homooligomers. Interacts with BMP2; can form higher oligomers with BMP2 (By similarity). Interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000097170

Structurei

3D structure databases

ProteinModelPortaliO70326
SMRiO70326
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini94 – 184CTCKAdd BLAST91

Sequence similaritiesi

Belongs to the DAN family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IG3Z Eukaryota
ENOG4111HEF LUCA
GeneTreeiENSGT00510000046909
HOVERGENiHBG051837
InParanoidiO70326
OMAiRVKECRC
OrthoDBiEOG091G0GL9
TreeFamiTF106445

Family and domain databases

Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR006207 Cys_knot_C
IPR029034 Cystine-knot_cytokine
IPR004133 DAN
IPR017159 Gremlin-1/2
PfamiView protein in Pfam
PF03045 DAN, 1 hit
PIRSFiPIRSF037254 Gremlin_precursor, 1 hit
SMARTiView protein in SMART
SM00041 CT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70326-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNRTAYTVGA LLLLLGTLLP TAEGKKKGSQ GAIPPPDKAQ HNDSEQTQSP
60 70 80 90 100
PQPGSRTRGR GQGRGTAMPG EEVLESSQEA LHVTERKYLK RDWCKTQPLK
110 120 130 140 150
QTIHEEGCNS RTIINRFCYG QCNSFYIPRH IRKEEGSFQS CSFCKPKKFT
160 170 180
TMMVTLNCPE LQPPTKKKRV TRVKQCRCIS IDLD
Length:184
Mass (Da):20,710
Last modified:August 1, 1998 - v1
Checksum:iD1BD99783CDC0F2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045801 mRNA Translation: AAC40111.1
AF108189 mRNA Translation: AAD54056.1
BC015293 mRNA Translation: AAH15293.1
CCDSiCCDS16560.1
RefSeqiNP_035954.1, NM_011824.4
UniGeneiMm.166318

Genome annotation databases

EnsembliENSMUST00000099575; ENSMUSP00000097170; ENSMUSG00000074934
GeneIDi23892
KEGGimmu:23892
UCSCiuc008lpp.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045801 mRNA Translation: AAC40111.1
AF108189 mRNA Translation: AAD54056.1
BC015293 mRNA Translation: AAH15293.1
CCDSiCCDS16560.1
RefSeqiNP_035954.1, NM_011824.4
UniGeneiMm.166318

3D structure databases

ProteinModelPortaliO70326
SMRiO70326
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000097170

PTM databases

iPTMnetiO70326
PhosphoSitePlusiO70326

Proteomic databases

PaxDbiO70326
PeptideAtlasiO70326
PRIDEiO70326

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099575; ENSMUSP00000097170; ENSMUSG00000074934
GeneIDi23892
KEGGimmu:23892
UCSCiuc008lpp.2 mouse

Organism-specific databases

CTDi26585
MGIiMGI:1344337 Grem1

Phylogenomic databases

eggNOGiENOG410IG3Z Eukaryota
ENOG4111HEF LUCA
GeneTreeiENSGT00510000046909
HOVERGENiHBG051837
InParanoidiO70326
OMAiRVKECRC
OrthoDBiEOG091G0GL9
TreeFamiTF106445

Miscellaneous databases

PROiPR:O70326
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074934 Expressed in 148 organ(s), highest expression level in fibroblast
CleanExiMM_GREM1
ExpressionAtlasiO70326 baseline and differential
GenevisibleiO70326 MM

Family and domain databases

Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR006207 Cys_knot_C
IPR029034 Cystine-knot_cytokine
IPR004133 DAN
IPR017159 Gremlin-1/2
PfamiView protein in Pfam
PF03045 DAN, 1 hit
PIRSFiPIRSF037254 Gremlin_precursor, 1 hit
SMARTiView protein in SMART
SM00041 CT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGREM1_MOUSE
AccessioniPrimary (citable) accession number: O70326
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: August 1, 1998
Last modified: September 12, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again