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Protein

Integrin beta-5

Gene

Itgb5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.

GO - Molecular functioni

  • integrin binding Source: BHF-UCL
  • signaling receptor activity Source: InterPro
  • signaling receptor binding Source: MGI

GO - Biological processi

  • cell adhesion mediated by integrin Source: GO_Central
  • cell-matrix adhesion Source: MGI
  • cell migration Source: GO_Central
  • epithelial cell-cell adhesion Source: MGI
  • integrin-mediated signaling pathway Source: UniProtKB
  • stress fiber assembly Source: UniProtKB
  • transforming growth factor beta receptor signaling pathway Source: UniProtKB

Keywordsi

Molecular functionIntegrin, Receptor
Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-5
Gene namesi
Name:Itgb5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96614 Itgb5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 719ExtracellularSequence analysisAdd BLAST696
Transmembranei720 – 742HelicalSequence analysisAdd BLAST23
Topological domaini743 – 798CytoplasmicSequence analysisAdd BLAST56

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001634924 – 798Integrin beta-5Add BLAST775

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi28 ↔ 463By similarity
Disulfide bondi36 ↔ 46By similarity
Disulfide bondi39 ↔ 75By similarity
Disulfide bondi49 ↔ 64By similarity
Disulfide bondi202 ↔ 211By similarity
Disulfide bondi259 ↔ 300By similarity
Glycosylationi347N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi401 ↔ 413By similarity
Disulfide bondi433 ↔ 682By similarity
Glycosylationi460N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi461 ↔ 465By similarity
Disulfide bondi476 ↔ 487By similarity
Glycosylationi479N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi484 ↔ 522By similarity
Disulfide bondi489 ↔ 498By similarity
Disulfide bondi500 ↔ 513By similarity
Glycosylationi505N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi528 ↔ 533By similarity
Disulfide bondi530 ↔ 563By similarity
Disulfide bondi535 ↔ 548By similarity
Disulfide bondi550 ↔ 555By similarity
Disulfide bondi569 ↔ 574By similarity
Disulfide bondi571 ↔ 602By similarity
Disulfide bondi576 ↔ 585By similarity
Glycosylationi586N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi587 ↔ 594By similarity
Disulfide bondi608 ↔ 613By similarity
Disulfide bondi610 ↔ 657By similarity
Disulfide bondi615 ↔ 625By similarity
Disulfide bondi628 ↔ 631By similarity
Disulfide bondi635 ↔ 644By similarity
Disulfide bondi641 ↔ 714By similarity
Glycosylationi654N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi661 ↔ 690By similarity
Glycosylationi705N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei770PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiO70309
MaxQBiO70309
PaxDbiO70309
PeptideAtlasiO70309
PRIDEiO70309

PTM databases

iPTMnetiO70309
PhosphoSitePlusiO70309

Expressioni

Gene expression databases

CleanExiMM_ITGB5

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Beta-5 (ITGB5) associates with alpha-V (ITGAV) (Probable). Interacts with MYO10 (By similarity). Interacts with DAB2. Integrin ITGAV:ITGB5 interacts with FBLN5 (via N-terminus) (PubMed:11805835). ITGAV:ITGB5 interacts with NOV (By similarity).By similarity1 Publication2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Myo7aP974793EBI-8401821,EBI-1149557

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200832, 2 interactors
ComplexPortaliCPX-3131 Integrin alphav-beta5 complex
IntActiO70309, 2 interactors
MINTiO70309
STRINGi10090.ENSMUSP00000069416

Structurei

3D structure databases

ProteinModelPortaliO70309
SMRiO70309
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 76PSIAdd BLAST50
Domaini136 – 378VWFAAdd BLAST243
Repeati465 – 512IAdd BLAST48
Repeati513 – 554IIAdd BLAST42
Repeati555 – 593IIIAdd BLAST39
Repeati594 – 630IVAdd BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni465 – 630Cysteine-rich tandem repeatsAdd BLAST166

Sequence similaritiesi

Belongs to the integrin beta chain family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1226 Eukaryota
ENOG410XP60 LUCA
HOGENOMiHOG000252936
HOVERGENiHBG006190
InParanoidiO70309
KOiK06588

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR027067 Integrin_beta-5
IPR033760 Integrin_beta_N
IPR015812 Integrin_bsu
IPR014836 Integrin_bsu_cyt_dom
IPR012896 Integrin_bsu_tail
IPR036349 Integrin_bsu_tail_dom_sf
IPR002369 Integrin_bsu_VWA
IPR032695 Integrin_dom_sf
IPR016201 PSI
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PANTHERiPTHR10082 PTHR10082, 1 hit
PTHR10082:SF26 PTHR10082:SF26, 1 hit
PfamiView protein in Pfam
PF08725 Integrin_b_cyt, 1 hit
PF07965 Integrin_B_tail, 1 hit
PF00362 Integrin_beta, 1 hit
PF17205 PSI_integrin, 1 hit
PIRSFiPIRSF002512 Integrin_B, 1 hit
PRINTSiPR01186 INTEGRINB
SMARTiView protein in SMART
SM00187 INB, 1 hit
SM01241 Integrin_b_cyt, 1 hit
SM01242 Integrin_B_tail, 1 hit
SM00423 PSI, 1 hit
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
SSF69179 SSF69179, 1 hit
SSF69687 SSF69687, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01186 EGF_2, 2 hits
PS00243 INTEGRIN_BETA, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform Beta-5A (identifier: O70309-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPRVPATLYA CLLGLCALVP RLAGLNICTS GSATSCEECL LIHPKCAWCS
60 70 80 90 100
KEYFGNPRSI TSRCDLKANL IRNGCEGEIE SPASSTHVLR NLPLSSKGSS
110 120 130 140 150
ATGSDVIQMT PQEIAVSLRP GEQTTFQLQV RQVEDYPVDL YYLMDLSLSM
160 170 180 190 200
KDDLENIRSL GTKLAEEMRK LTSNFRLGFG SFVDKDISPF SYTAPRYQTN
210 220 230 240 250
PCIGYKLFPN CVPSFGFRHL LPLTDRVDSF NEEVRKQRVS RNRDAPEGGF
260 270 280 290 300
DAVLQAAVCK EKIGWRKDAL HLLVFTTDDV PHIALDGKLG GLVQPHDGQC
310 320 330 340 350
HLNEANEYTA SNQMDYPSLA LLGEKLAENN INLIFAVTKN HYMLYKNFTA
360 370 380 390 400
LIPGTTVEIL HGDSKNIIQL IINAYSSIRA KVELSVWDQP EDLNLFFTAT
410 420 430 440 450
CQDGISYPGQ RKCEGLKIGD TASFEVSVEA RSCPGRQAAQ SFTLRPVGFR
460 470 480 490 500
DSLQVEVAYN CTCGCSTGLE PNSARCSGNG TYTCGLCECD PGYLGTRCEC
510 520 530 540 550
QEGENQSGYQ NLCREAEGKP LCSGRGECSC NQCSCFESEF GRIYGPFCEC
560 570 580 590 600
DSFSCARNKG VLCSGHGECH CGECKCHAGY IGDNCNCSTD VSTCKAKDGQ
610 620 630 640 650
ICSDRGRCVC GQCQCTEPGA FGETCEKCPT CPDACSSKRD CVECLLLHQG
660 670 680 690 700
KPDNQTCHHQ CKDEVITWVD TIVKDDQEAV LCFYKTAKDC VMMFSYTELP
710 720 730 740 750
NGRSNLTVLR EPECGSAPNA MTILLAVVGS ILLIGMALLA IWKLLVTIHD
760 770 780 790
RREFAKFQSE RSRARYEMAS NPLYRKPIST HTVDFAFNKF NKSYNGSV
Length:798
Mass (Da):87,909
Last modified:April 27, 2001 - v2
Checksum:i34B9D8B07E2688B0
GO
Isoform Beta-5B (identifier: O70309-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     760-798: ERSRARYEMA...KFNKSYNGSV → LKPPVQKAHL...PWTSWTICSR

Show »
Length:816
Mass (Da):90,035
Checksum:i8ED736C097EBA855
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G5E8F8G5E8F8_MOUSE
Integrin beta
Itgb5 mCG_127131
816Annotation score:
Q6PE70Q6PE70_MOUSE
Integrin beta
Itgb5
799Annotation score:
A0A338P795A0A338P795_MOUSE
Integrin beta
Itgb5
486Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96S → C in AAD08782 (PubMed:9880508).Curated1
Sequence conflicti259C → Y in AAD08782 (PubMed:9880508).Curated1
Sequence conflicti595K → R in AAD08782 (PubMed:9880508).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002752760 – 798ERSRA…YNGSV → LKPPVQKAHLHTHCRFRLQQ VQQILQWLSGLRLLDGWRGT KDEDSGVPWTSWTICSR in isoform Beta-5B. 1 PublicationAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043257 mRNA Translation: AAC40110.1
AF043256 mRNA Translation: AAC40109.1
AF022110 mRNA Translation: AAD08782.1
CCDSiCCDS28135.1 [O70309-2]
RefSeqiNP_001139356.1, NM_001145884.1
NP_034710.2, NM_010580.2
UniGeneiMm.6424

Genome annotation databases

GeneIDi16419
KEGGimmu:16419

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043257 mRNA Translation: AAC40110.1
AF043256 mRNA Translation: AAC40109.1
AF022110 mRNA Translation: AAD08782.1
CCDSiCCDS28135.1 [O70309-2]
RefSeqiNP_001139356.1, NM_001145884.1
NP_034710.2, NM_010580.2
UniGeneiMm.6424

3D structure databases

ProteinModelPortaliO70309
SMRiO70309
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200832, 2 interactors
ComplexPortaliCPX-3131 Integrin alphav-beta5 complex
IntActiO70309, 2 interactors
MINTiO70309
STRINGi10090.ENSMUSP00000069416

PTM databases

iPTMnetiO70309
PhosphoSitePlusiO70309

Proteomic databases

EPDiO70309
MaxQBiO70309
PaxDbiO70309
PeptideAtlasiO70309
PRIDEiO70309

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi16419
KEGGimmu:16419

Organism-specific databases

CTDi3693
MGIiMGI:96614 Itgb5

Phylogenomic databases

eggNOGiKOG1226 Eukaryota
ENOG410XP60 LUCA
HOGENOMiHOG000252936
HOVERGENiHBG006190
InParanoidiO70309
KOiK06588

Miscellaneous databases

ChiTaRSiItgb5 mouse
PROiPR:O70309
SOURCEiSearch...

Gene expression databases

CleanExiMM_ITGB5

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR027067 Integrin_beta-5
IPR033760 Integrin_beta_N
IPR015812 Integrin_bsu
IPR014836 Integrin_bsu_cyt_dom
IPR012896 Integrin_bsu_tail
IPR036349 Integrin_bsu_tail_dom_sf
IPR002369 Integrin_bsu_VWA
IPR032695 Integrin_dom_sf
IPR016201 PSI
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PANTHERiPTHR10082 PTHR10082, 1 hit
PTHR10082:SF26 PTHR10082:SF26, 1 hit
PfamiView protein in Pfam
PF08725 Integrin_b_cyt, 1 hit
PF07965 Integrin_B_tail, 1 hit
PF00362 Integrin_beta, 1 hit
PF17205 PSI_integrin, 1 hit
PIRSFiPIRSF002512 Integrin_B, 1 hit
PRINTSiPR01186 INTEGRINB
SMARTiView protein in SMART
SM00187 INB, 1 hit
SM01241 Integrin_b_cyt, 1 hit
SM01242 Integrin_B_tail, 1 hit
SM00423 PSI, 1 hit
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
SSF69179 SSF69179, 1 hit
SSF69687 SSF69687, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 2 hits
PS01186 EGF_2, 2 hits
PS00243 INTEGRIN_BETA, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiITB5_MOUSE
AccessioniPrimary (citable) accession number: O70309
Secondary accession number(s): O70308, O88347
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: November 7, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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