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Protein

G protein-coupled receptor kinase 4

Gene

Grk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically phosphorylates the activated forms of G protein-coupled receptors.

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

Activity regulationi

Inhibited by heparin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215ATPPROSITE-ProRule annotation1
Active sitei311Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi192 – 200ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 4 (EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase GRK4
Gene namesi
Name:Grk4
Synonyms:Gprk2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:95801 Grk4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859681 – 574G protein-coupled receptor kinase 4Add BLAST574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei484PhosphoserineBy similarity1

Post-translational modificationi

Palmitoylated.By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiO70291
PaxDbiO70291
PeptideAtlasiO70291
PRIDEiO70291

PTM databases

PhosphoSitePlusiO70291

Expressioni

Gene expression databases

BgeeiENSMUSG00000052783 Expressed in 83 organ(s), highest expression level in testis
CleanExiMM_GRK4
ExpressionAtlasiO70291 baseline and differential
GenevisibleiO70291 MM

Interactioni

Subunit structurei

Interacts with DRD3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001112

Structurei

3D structure databases

ProteinModelPortaliO70291
SMRiO70291
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 171RGSPROSITE-ProRule annotationAdd BLAST121
Domaini186 – 448Protein kinasePROSITE-ProRule annotationAdd BLAST263
Domaini449 – 514AGC-kinase C-terminalAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 153N-terminalAdd BLAST153

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0986 Eukaryota
ENOG410YRQZ LUCA
GeneTreeiENSGT00860000133699
HOGENOMiHOG000006742
HOVERGENiHBG004532
InParanoidiO70291
KOiK08291
OMAiHSIEKDY
OrthoDBiEOG091G062G
TreeFamiTF313940

Family and domain databases

InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000239 GPCR_kinase
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR016137 RGS
IPR036305 RGS_sf
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR00717 GPCRKINASE
SMARTiView protein in SMART
SM00315 RGS, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50132 RGS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O70291-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELENFVANN LLLKARLGFN KQTGRSKKWR ELLKFPPVSM CTELRWSIEK
60 70 80 90 100
DFSSLCDKQP IGRLLFRQFC DTKPDLKRCI EFLDAVAEYE VTIEEEQREF
110 120 130 140 150
GLAIFSRFFK EKSEVPLPEI PPDIVKECKW NLKQNSPSQN VFEECAGIVC
160 170 180 190 200
KYLSETPFEE YQESTYFNRF LQWKWLERRP VTKNTFRQYR VLGKGGFGEV
210 220 230 240 250
CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKRILEK LHSRFVVSLA
260 270 280 290 300
YTYETKDALC LVLTIMNGGD LKYHIYNLGD PGFEEPRAVF YAAELCCGLE
310 320 330 340 350
DLQRKRIVYR DLKPENILLD DHGHIRISDL GLAMEVPEGE MVRGRVGTVG
360 370 380 390 400
YMAPEIINHE KYTFSPDWWG LGCLIYEMIA GHSPFRKYKE KVNREELERR
410 420 430 440 450
VKNETEEYSE RFSEDAKSIC SMLLIKDPSK RLGCQRDGVS AVKQHPIFKD
460 470 480 490 500
INFSRLEANM LDPPFIPDPQ AIYCRNILDI GQFSVVKGVN LDTNDEIFYA
510 520 530 540 550
EFATGSVTIP WQNEMIESGC FKDLNENEDD LSSLEKYKMC SSILRPKRNF
560 570
FRRLFRRTGC LNIALSEERE PTEH
Length:574
Mass (Da):66,928
Last modified:July 27, 2011 - v2
Checksum:i7D7D6F46C9C044B6
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8BP13Q8BP13_MOUSE
G protein-coupled receptor kinase 4
Grk4 Gprk2l
177Annotation score:
F7BEP0F7BEP0_MOUSE
G protein-coupled receptor kinase 4
Grk4
96Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6F → M in AAC09266 (PubMed:10506199).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040745 mRNA Translation: AAC09266.1
AK132685 mRNA Translation: BAE21301.1
CH466524 Genomic DNA Translation: EDL37466.1
BC150691 mRNA Translation: AAI50692.1
CCDSiCCDS19219.1
RefSeqiNP_001074212.1, NM_001080743.1
NP_062370.2, NM_019497.2
UniGeneiMm.117076

Genome annotation databases

EnsembliENSMUST00000001112; ENSMUSP00000001112; ENSMUSG00000052783
GeneIDi14772
KEGGimmu:14772
UCSCiuc008xcz.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF040745 mRNA Translation: AAC09266.1
AK132685 mRNA Translation: BAE21301.1
CH466524 Genomic DNA Translation: EDL37466.1
BC150691 mRNA Translation: AAI50692.1
CCDSiCCDS19219.1
RefSeqiNP_001074212.1, NM_001080743.1
NP_062370.2, NM_019497.2
UniGeneiMm.117076

3D structure databases

ProteinModelPortaliO70291
SMRiO70291
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001112

PTM databases

PhosphoSitePlusiO70291

Proteomic databases

MaxQBiO70291
PaxDbiO70291
PeptideAtlasiO70291
PRIDEiO70291

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001112; ENSMUSP00000001112; ENSMUSG00000052783
GeneIDi14772
KEGGimmu:14772
UCSCiuc008xcz.1 mouse

Organism-specific databases

CTDi2868
MGIiMGI:95801 Grk4

Phylogenomic databases

eggNOGiKOG0986 Eukaryota
ENOG410YRQZ LUCA
GeneTreeiENSGT00860000133699
HOGENOMiHOG000006742
HOVERGENiHBG004532
InParanoidiO70291
KOiK08291
OMAiHSIEKDY
OrthoDBiEOG091G062G
TreeFamiTF313940

Enzyme and pathway databases

ReactomeiR-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade

Miscellaneous databases

PROiPR:O70291
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052783 Expressed in 83 organ(s), highest expression level in testis
CleanExiMM_GRK4
ExpressionAtlasiO70291 baseline and differential
GenevisibleiO70291 MM

Family and domain databases

InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR000239 GPCR_kinase
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR016137 RGS
IPR036305 RGS_sf
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR00717 GPCRKINASE
SMARTiView protein in SMART
SM00315 RGS, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50132 RGS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGRK4_MOUSE
AccessioniPrimary (citable) accession number: O70291
Secondary accession number(s): Q3V151
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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