UniProtKB - O70283 (WNT2B_MOUSE)
Protein
Protein Wnt-2b
Gene
Wnt2b
Organism
Mus musculus (Mouse)
Status
Functioni
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:19686689). Plays a redundant role in embryonic lung development (PubMed:19686689).Curated1 Publication
GO - Molecular functioni
- cytokine activity Source: GO_Central
- frizzled binding Source: GO_Central
- signaling receptor binding Source: MGI
GO - Biological processi
- animal organ morphogenesis Source: MGI
- canonical Wnt signaling pathway Source: MGI
- cell-cell signaling Source: MGI
- cell fate commitment Source: GO_Central
- cellular response to starvation Source: MGI
- chondrocyte differentiation Source: Ensembl
- forebrain regionalization Source: UniProtKB
- hematopoietic stem cell proliferation Source: MGI
- lung induction Source: MGI
- male gonad development Source: Ensembl
- mesenchymal-epithelial cell signaling Source: MGI
- neuron differentiation Source: GO_Central
- positive regulation of branching involved in ureteric bud morphogenesis Source: MGI
- signal transduction Source: MGI
Keywordsi
Molecular function | Developmental protein |
Biological process | Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-MMU-3238698, WNT ligand biogenesis and trafficking |
Names & Taxonomyi
Protein namesi | Recommended name: Protein Wnt-2bAlternative name(s): Protein Wnt-131 Publication |
Gene namesi | Name:Wnt2b Synonyms:Wnt131 Publication |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1261834, Wnt2b |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix By similarity
- Secreted By similarity
Extracellular region or secreted
- extracellular space Source: GO_Central
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Extracellular matrix, SecretedPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – ? | Sequence analysis | ||
ChainiPRO_0000041414 | ? – 389 | Protein Wnt-2b |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 105 ↔ 116 | By similarity | ||
Glycosylationi | 115 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 156 ↔ 164 | By similarity | ||
Disulfide bondi | 166 ↔ 186 | By similarity | ||
Disulfide bondi | 235 ↔ 249 | By similarity | ||
Disulfide bondi | 237 ↔ 244 | By similarity | ||
Lipidationi | 241 | O-palmitoleoyl serine; by PORCNBy similarity | 1 | |
Glycosylationi | 281 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 307 ↔ 338 | By similarity | ||
Disulfide bondi | 323 ↔ 333 | By similarity | ||
Disulfide bondi | 337 ↔ 377 | By similarity | ||
Disulfide bondi | 353 ↔ 368 | By similarity | ||
Disulfide bondi | 355 ↔ 365 | By similarity | ||
Disulfide bondi | 360 ↔ 361 | By similarity |
Post-translational modificationi
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, LipoproteinProteomic databases
MaxQBi | O70283 |
PaxDbi | O70283 |
PRIDEi | O70283 |
PTM databases
GlyGeni | O70283, 2 sites |
iPTMneti | O70283 |
PhosphoSitePlusi | O70283 |
Expressioni
Developmental stagei
Detected at the dorsal midline at the level of the diencephalon and mesencephalon at 9.5 dpc. Detected at the level of the optic and otic vesicles at 9.5 dpc (PubMed:9545553). Detected in the lateral plate mesoderm surrounding the ventral aspect of the anterior foregut at 9.5 dpc (PubMed:19686689). Detected in the mesothelium encasing the lung, and at lower levels in the distal mesenchyme from 12.5 dpc to 14.5 dpc (PubMed:19686689).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000027840, Expressed in embryo and 141 other tissues |
Genevisiblei | O70283, MM |
Interactioni
Subunit structurei
Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity. The complex with AFM may represent the physiological form in body fluids (By similarity).
Interacts with FZD4 and FZD5 (By similarity).
By similarityGO - Molecular functioni
- cytokine activity Source: GO_Central
- frizzled binding Source: GO_Central
- signaling receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204573, 1 interactor |
STRINGi | 10090.ENSMUSP00000029429 |
Miscellaneous databases
RNActi | O70283, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the Wnt family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3913, Eukaryota |
GeneTreei | ENSGT00940000159166 |
HOGENOMi | CLU_033039_1_0_1 |
InParanoidi | O70283 |
OMAi | HPDIMQA |
OrthoDBi | 745245at2759 |
TreeFami | TF105310 |
Family and domain databases
Gene3Di | 3.30.2460.20, 1 hit |
InterProi | View protein in InterPro IPR005817, Wnt IPR009140, Wnt2 IPR043158, Wnt_C IPR018161, Wnt_CS |
PANTHERi | PTHR12027, PTHR12027, 1 hit |
Pfami | View protein in Pfam PF00110, wnt, 1 hit |
PRINTSi | PR01842, WNT2PROTEIN PR01349, WNTPROTEIN |
SMARTi | View protein in SMART SM00097, WNT1, 1 hit |
PROSITEi | View protein in PROSITE PS00246, WNT1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O70283-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKLQGEDEA AQLAPRRARV PVPRPTAPDV SPSSARLGLA CLLLLLLLTL
60 70 80 90 100
PARVDTSWWY IGALGARVIC DNIPGLVSRQ RQLCQRYPDI MRSVGEGARE
110 120 130 140 150
WIRECQHQFR HHRWNCTTLD RDHTVFGRAM LRSSREAAFV YAISSAGVVH
160 170 180 190 200
AITRACSQGE LSVCSCDPYT RGRHHDQRGD FDWGGCSDNI HYGVRFAKAF
210 220 230 240 250
VDAKEKRLKD ARALMNLHNN RCGRTAVRRF LKLECKCHGV SGSCTLRTCW
260 270 280 290 300
RALSDFRRTG DYLRRRYDGA VQVTATQDGA NFTAARQGYR HATRTDLVYF
310 320 330 340 350
DNSPDYCVLD KAAGSLGTAG RVCSKTSKGT DGCEIMCCGR GYDTTRVTRV
360 370 380
TQCECKFHWC CAVRCKECRN TVDVHTCKAP KKAEWLDQT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 313 | A → S in AAC25397 (PubMed:9545553).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF070988 mRNA Translation: AAC25397.1 AK035653 mRNA Translation: BAC29139.1 CH466608 Genomic DNA Translation: EDL07557.1 BC119276 mRNA Translation: AAI19277.1 BC119278 mRNA Translation: AAI19279.1 AF038384 mRNA Translation: AAC40123.1 |
CCDSi | CCDS17706.1 |
RefSeqi | NP_033546.2, NM_009520.3 |
Genome annotation databases
Ensembli | ENSMUST00000029429; ENSMUSP00000029429; ENSMUSG00000027840 |
GeneIDi | 22414 |
KEGGi | mmu:22414 |
UCSCi | uc008qut.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF070988 mRNA Translation: AAC25397.1 AK035653 mRNA Translation: BAC29139.1 CH466608 Genomic DNA Translation: EDL07557.1 BC119276 mRNA Translation: AAI19277.1 BC119278 mRNA Translation: AAI19279.1 AF038384 mRNA Translation: AAC40123.1 |
CCDSi | CCDS17706.1 |
RefSeqi | NP_033546.2, NM_009520.3 |
3D structure databases
SMRi | O70283 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204573, 1 interactor |
STRINGi | 10090.ENSMUSP00000029429 |
PTM databases
GlyGeni | O70283, 2 sites |
iPTMneti | O70283 |
PhosphoSitePlusi | O70283 |
Proteomic databases
MaxQBi | O70283 |
PaxDbi | O70283 |
PRIDEi | O70283 |
Protocols and materials databases
Antibodypediai | 20129, 237 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000029429; ENSMUSP00000029429; ENSMUSG00000027840 |
GeneIDi | 22414 |
KEGGi | mmu:22414 |
UCSCi | uc008qut.1, mouse |
Organism-specific databases
CTDi | 7482 |
MGIi | MGI:1261834, Wnt2b |
Phylogenomic databases
eggNOGi | KOG3913, Eukaryota |
GeneTreei | ENSGT00940000159166 |
HOGENOMi | CLU_033039_1_0_1 |
InParanoidi | O70283 |
OMAi | HPDIMQA |
OrthoDBi | 745245at2759 |
TreeFami | TF105310 |
Enzyme and pathway databases
Reactomei | R-MMU-3238698, WNT ligand biogenesis and trafficking |
Miscellaneous databases
BioGRID-ORCSi | 22414, 2 hits in 18 CRISPR screens |
ChiTaRSi | Wnt2b, mouse |
PROi | PR:O70283 |
RNActi | O70283, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027840, Expressed in embryo and 141 other tissues |
Genevisiblei | O70283, MM |
Family and domain databases
Gene3Di | 3.30.2460.20, 1 hit |
InterProi | View protein in InterPro IPR005817, Wnt IPR009140, Wnt2 IPR043158, Wnt_C IPR018161, Wnt_CS |
PANTHERi | PTHR12027, PTHR12027, 1 hit |
Pfami | View protein in Pfam PF00110, wnt, 1 hit |
PRINTSi | PR01842, WNT2PROTEIN PR01349, WNTPROTEIN |
SMARTi | View protein in SMART SM00097, WNT1, 1 hit |
PROSITEi | View protein in PROSITE PS00246, WNT1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | WNT2B_MOUSE | |
Accessioni | O70283Primary (citable) accession number: O70283 Secondary accession number(s): O88530, Q544L3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families