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Protein

Protein-tyrosine sulfotransferase 1

Gene

Tpst1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100Proton donor/acceptorBy similarity1
Sitei159Transition state stabilizerBy similarity1
Binding sitei184PAPSBy similarity1
Binding sitei192PAPSBy similarity1
Binding sitei196PAPSBy similarity1
Binding sitei239PAPSBy similarity1
Sitei286Transition state stabilizerBy similarity1
Binding sitei301PAPSBy similarity1

GO - Molecular functioni

GO - Biological processi

  • peptidyl-tyrosine sulfation Source: UniProtKB

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.8.2.20 3474
ReactomeiR-MMU-156584 Cytosolic sulfonation of small molecules

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine sulfotransferase 1 (EC:2.8.2.201 Publication)
Alternative name(s):
Tyrosylprotein sulfotransferase 1
Short name:
TPST-1
Gene namesi
Name:Tpst1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1298231 Tpst1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini26 – 370LumenalSequence analysisAdd BLAST345

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001898271 – 370Protein-tyrosine sulfotransferase 1Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi97 ↔ 157By similarity
Disulfide bondi226 ↔ 234By similarity
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO70281
PeptideAtlasiO70281
PRIDEiO70281

PTM databases

iPTMnetiO70281
PhosphoSitePlusiO70281

Expressioni

Tissue specificityi

Ubiquitous. Detected in heart, brain, lung, liver, spleen, kidney, skeletal muscle and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000034118
CleanExiMM_TPST1
ExpressionAtlasiO70281 baseline and differential
GenevisibleiO70281 MM

Interactioni

Subunit structurei

Homodimer. Can also form heterodimers with TPST2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035614

Structurei

3D structure databases

ProteinModelPortaliO70281
SMRiO70281
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 83PAPS bindingBy similarity5
Regioni102 – 106Interaction with peptide substrateBy similarity5
Regioni286 – 295PAPS bindingBy similarity10

Sequence similaritiesi

Belongs to the protein sulfotransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3988 Eukaryota
ENOG410XQ2X LUCA
GeneTreeiENSGT00390000006030
HOGENOMiHOG000253020
HOVERGENiHBG001270
InParanoidiO70281
KOiK01021
OMAiHIPWNHS
OrthoDBiEOG091G09YJ
PhylomeDBiO70281
TreeFamiTF312910

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR026634 TPST
PANTHERiPTHR12788 PTHR12788, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits

Sequencei

Sequence statusi: Complete.

O70281-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGKLKQNLL LACLVISSVT VFYLGQHAME CHHRIEERSQ PARLENPKAT
60 70 80 90 100
VRAGLDIKAN KTFTYHKDMP LIFIGGVPRS GTTLMRAMLD AHPDIRCGEE
110 120 130 140 150
TRVIPRILAL KQMWSRSSKE KIRLDEAGVT DEVLDSAMQA FLLEVIVKHG
160 170 180 190 200
EPAPYLCNKD PFALKSLTYL ARLFPNAKFL LMVRDGRASV HSMISRKVTI
210 220 230 240 250
AGFDLNSYRD CLTKWNRAIE TMYNQCMEVG YKKCMLVHYE QLVLHPERWM
260 270 280 290 300
RTLLKFLHIP WNHSVLHHEE MIGKAGGVSL SKVERSTDQV IKPVNVGALS
310 320 330 340 350
KWVGKIPPDV LQDMAVIAPM LAKLGYDPYA NPPNYGKPDP KILENTRRVY
360 370
KGEFQLPDFL KEKPQTEQVE
Length:370
Mass (Da):42,132
Last modified:August 1, 1998 - v1
Checksum:i4B85624D23C7800D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038008 mRNA Translation: AAC13551.1
BC012666 mRNA Translation: AAH12666.1
CCDSiCCDS39293.1
RefSeqiNP_001123948.1, NM_001130476.2
NP_038865.1, NM_013837.2
UniGeneiMm.332720

Genome annotation databases

EnsembliENSMUST00000040721; ENSMUSP00000035614; ENSMUSG00000034118
ENSMUST00000118993; ENSMUSP00000112571; ENSMUSG00000034118
GeneIDi22021
KEGGimmu:22021
UCSCiuc008zuc.3 mouse

Similar proteinsi

Entry informationi

Entry nameiTPST1_MOUSE
AccessioniPrimary (citable) accession number: O70281
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: December 20, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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