UniProtKB - O70273 (EHF_MOUSE)

BLAST

>sp|O70273|EHF_MOUSE ETS homologous factor OS=Mus musculus OX=10090 GN=Ehf PE=2 SV=1
MILEGSGVMNLNPANNLLHQQPAWTDSYPTCNVSSGFFGSQWHEIHPQYWTKYQVWEWLQ
HLLDTNQLDASCIPFQEFDISGEHLCSMSLQEFTRAAGSAGQLLYSNLQHLKWNGQCSSD
LFQSAHNVIVKTEQTDPSIMNTWKEENYLYDPSYGSTVDLLDSKTFCRAQISMTTSSHLP
VAESPDMKKEQDHPVKSHTKKHNPRGTHLWEFIRDILLSPDKNPGLIKWEDRSEGIFRFL
KSEAVAQLWGKKKNNSSMTYEKLSRAMRYYYKREILERVDGRRLVYKFGKNARGWRENEN

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History
Entry version 134 (07 Nov 2018)
Sequence version 1 (01 Aug 1998)
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Protein

ETS homologous factor

Gene

Ehf

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at transcript levelⁱ

Functionⁱ

Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes, and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter (By similarity).By similarity1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	207 – 289	ETSPROSITE-ProRule annotationAdd BLAST		83

GO - Molecular functionⁱ

DNA binding Source: UniProtKB
DNA-binding transcription factor activity Source: UniProtKB
DNA-binding transcription factor activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell differentiation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
epithelial cell differentiation Source: UniProtKB
epithelial cell proliferation Source: InterPro
positive regulation of transcription, DNA-templated Source: UniProtKB
positive regulation of transcription by RNA polymerase II Source: MGI
regulation of transcription by RNA polymerase II
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
transcription by RNA polymerase II Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ETS homologous factor Alternative name(s): ETS domain-containing transcription factor
Gene namesⁱ	Name:EhfImported
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 2

Organism-specific databases

MGIⁱ	MGI:1270840 Ehf

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000257970	1 – 300	ETS homologous factorAdd BLAST		300

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O70273
PaxDbⁱ	O70273
PeptideAtlas More...PeptideAtlasⁱ	O70273
PRIDEⁱ	O70273

PTM databases

PhosphoSitePlusⁱ	O70273

PhosphoSitePlusⁱ

O70273

Expressionⁱ

Tissue specificityⁱ

Highly expressed in kidney and lung, weakly in skeletal muscle, heart, and liver, and not detected in brain, spleen or testis.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000012350 Expressed in 154 organ(s), highest expression level in submandibular gland
CleanExⁱ	MM_EHF
ExpressionAtlasⁱ	O70273 baseline and differential
Genevisibleⁱ	O70273 MM

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	199409, 1 interactor
STRINGⁱ	10090.ENSMUSP00000087961

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O70273
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	29 – 115	PNTPROSITE-ProRule annotationAdd BLAST		87

Domainⁱ

The PNT domain acts as a transcriptional activator.1 Publication

Sequence similaritiesⁱ

Belongs to the ETS family.Sequence analysis

Phylogenomic databases

eggNOGⁱ	KOG3804 Eukaryota ENOG4111K4J LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118996
HOGENOMⁱ	HOG000232188
HOVERGENⁱ	HBG074143
InParanoidⁱ	O70273
KEGG Orthology (KO) More...KOⁱ	K17102
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0BE6
PhylomeDBⁱ	O70273
TreeFamⁱ	TF318679

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR033071 EHF IPR000418 Ets_dom IPR003118 Pointed_dom IPR013761 SAM/pointed_sf IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
PANTHERⁱ	PTHR11849:SF171 PTHR11849:SF171, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00178 Ets, 1 hit PF02198 SAM_PNT, 1 hit
PRINTSⁱ	PR00454 ETSDOMAIN
SMARTⁱ	View protein in SMART SM00413 ETS, 1 hit SM00251 SAM_PNT, 1 hit
SUPFAMⁱ	SSF46785 SSF46785, 1 hit SSF47769 SSF47769, 1 hit
PROSITEⁱ	View protein in PROSITE PS50061 ETS_DOMAIN_3, 1 hit PS51433 PNT, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 11 Publication (identifier: O70273-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MILEGSGVMN LNPANNLLHQ QPAWTDSYPT CNVSSGFFGS QWHEIHPQYW 
        60         70         80         90        100
TKYQVWEWLQ HLLDTNQLDA SCIPFQEFDI SGEHLCSMSL QEFTRAAGSA 
       110        120        130        140        150
GQLLYSNLQH LKWNGQCSSD LFQSAHNVIV KTEQTDPSIM NTWKEENYLY 
       160        170        180        190        200
DPSYGSTVDL LDSKTFCRAQ ISMTTSSHLP VAESPDMKKE QDHPVKSHTK 
       210        220        230        240        250
KHNPRGTHLW EFIRDILLSP DKNPGLIKWE DRSEGIFRFL KSEAVAQLWG 
       260        270        280        290        300
KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN

Length:300

Mass (Da):34,903

Last modified:August 1, 1998 - v1

Checksum:ⁱ91D9477EFB874747

Isoform 21 Publication (identifier: O70273-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
79-93: Missing.

« Hide

        10         20         30         40         50
MILEGSGVMN LNPANNLLHQ QPAWTDSYPT CNVSSGFFGS QWHEIHPQYW 
        60         70         80         90        100
TKYQVWEWLQ HLLDTNQLDA SCIPFQEFTR AAGSAGQLLY SNLQHLKWNG 
       110        120        130        140        150
QCSSDLFQSA HNVIVKTEQT DPSIMNTWKE ENYLYDPSYG STVDLLDSKT 
       160        170        180        190        200
FCRAQISMTT SSHLPVAESP DMKKEQDHPV KSHTKKHNPR GTHLWEFIRD 
       210        220        230        240        250
ILLSPDKNPG LIKWEDRSEG IFRFLKSEAV AQLWGKKKNN SSMTYEKLSR 
       260        270        280 
AMRYYYKREI LERVDGRRLV YKFGKNARGW RENEN

Note: No experimental confirmation available.Curated

Show »

Length:285

Mass (Da):33,225

Checksum:ⁱ91398C527C65955F

Isoform 31 Publication (identifier: O70273-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
200-211: KKHNPRGTHLWE → YFIIELLLSESR
212-300: Missing.

« Hide

        10         20         30         40         50
MILEGSGVMN LNPANNLLHQ QPAWTDSYPT CNVSSGFFGS QWHEIHPQYW 
        60         70         80         90        100
TKYQVWEWLQ HLLDTNQLDA SCIPFQEFDI SGEHLCSMSL QEFTRAAGSA 
       110        120        130        140        150
GQLLYSNLQH LKWNGQCSSD LFQSAHNVIV KTEQTDPSIM NTWKEENYLY 
       160        170        180        190        200
DPSYGSTVDL LDSKTFCRAQ ISMTTSSHLP VAESPDMKKE QDHPVKSHTY 
       210 
FIIELLLSES R

Note: No experimental confirmation available.Curated

Show »

Length:211

Mass (Da):24,139

Checksum:ⁱ54F8846761D533CD

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

A2BIB5	A2BIB5_MOUSE	ETS homologous factor ETS homologous factor	Ehf	277	Annotation score:
B0S7M9	B0S7M9_MOUSE	ETS homologous factor ETS homologous factor	Ehf	242	Annotation score:
A2BIB6	A2BIB6_MOUSE	ETS homologous factor ETS homologous factor	Ehf	127	Annotation score:
A2BIB7	A2BIB7_MOUSE	ETS homologous factor ETS homologous factor	Ehf	181	Annotation score:

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_052191	79 – 93	Missing in isoform 2. 1 PublicationAdd BLAST	15
Alternative sequenceⁱVSP_052192	200 – 211	KKHNP…THLWE → YFIIELLLSESR in isoform 3. 1 PublicationAdd BLAST	12
Alternative sequenceⁱVSP_052193	212 – 300	Missing in isoform 3. 1 PublicationAdd BLAST	89

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF035527 mRNA Translation: AAC40119.1 BC005520 mRNA Translation: AAH05520.1 BC006789 mRNA Translation: AAH06789.1 BC008249 mRNA Translation: AAH08249.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS16475.1 [O70273-1]
PIRⁱ	JW0048
NCBI Reference Sequences More...RefSeqⁱ	NP_031940.1, NM_007914.3 [O70273-1] XP_006498760.1, XM_006498697.2 [O70273-1] XP_006498761.1, XM_006498698.3 [O70273-1] XP_006498762.1, XM_006498699.1 [O70273-1] XP_006498763.1, XM_006498700.3 [O70273-1] XP_006498764.1, XM_006498701.3 [O70273-1] XP_006498765.1, XM_006498702.3 [O70273-1]
UniGeneⁱ	Mm.10724

Genome annotation databases

Ensemblⁱ	ENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350 [O70273-1] ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350 [O70273-1]
GeneIDⁱ	13661
KEGGⁱ	mmu:13661
UCSC genome browser More...UCSCⁱ	uc008lio.1 mouse [O70273-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O70273	ETS homologous factor (Fragment)		MOUSE		127	UniRef100_O70273
UPI000A30DDA3		MUSPA		302
ETS homologous factor (Fragment)		MOUSE		242
UPI0003D74C28		MOUSE		311

Protein	Similar proteins		Species	Score	Length	Source
O70273	ETS homologous factor		HUMAN		300	UniRef90_Q9NZC4
ETS homologous factor		PANTR		300
ETS homologous factor		PANPA		300
Uncharacterized protein		AOTNA		324
EHF isoform 8 (Fragment)		PONAB		158
+209
O70273-2	ETS like proteinous factor		FUKDA		326	UniRef90_O70273-2
ETS homologous factor		DELLE		322
Uncharacterized protein		AOTNA		290
EHF (Fragment)		ATEGE		97
UPI00084799BF		CAPHI		322
+12

Protein	Similar proteins		Species	Score	Length	Source
O70273	ETS homologous factor		HUMAN		300	UniRef50_Q9NZC4
ETS homologous factor		PANTR		300
ETS homologous factor		PANPA		300
ETS homologous factor		BOVIN		300
Uncharacterized protein		AOTNA		324
+417
O70273-3	LOW QUALITY PROTEIN: ets homologous factor		LEPWE		228	UniRef50_O70273-3
UPI000951B15D		BOSIN		250
UPI0007608822		BOVIN		250

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF035527 mRNA Translation: AAC40119.1 BC005520 mRNA Translation: AAH05520.1 BC006789 mRNA Translation: AAH06789.1 BC008249 mRNA Translation: AAH08249.1
CCDSⁱ	CCDS16475.1 [O70273-1]
PIRⁱ	JW0048
RefSeqⁱ	NP_031940.1, NM_007914.3 [O70273-1] XP_006498760.1, XM_006498697.2 [O70273-1] XP_006498761.1, XM_006498698.3 [O70273-1] XP_006498762.1, XM_006498699.1 [O70273-1] XP_006498763.1, XM_006498700.3 [O70273-1] XP_006498764.1, XM_006498701.3 [O70273-1] XP_006498765.1, XM_006498702.3 [O70273-1]
UniGeneⁱ	Mm.10724

3D structure databases

ProteinModelPortalⁱ	O70273
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	199409, 1 interactor
STRINGⁱ	10090.ENSMUSP00000087961

PTM databases

PhosphoSitePlusⁱ	O70273

PhosphoSitePlusⁱ

O70273

Proteomic databases

MaxQBⁱ	O70273
PaxDbⁱ	O70273
PeptideAtlasⁱ	O70273
PRIDEⁱ	O70273

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350 [O70273-1] ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350 [O70273-1]
GeneIDⁱ	13661
KEGGⁱ	mmu:13661
UCSCⁱ	uc008lio.1 mouse [O70273-1]

Organism-specific databases

CTDⁱ	26298
MGIⁱ	MGI:1270840 Ehf

Phylogenomic databases

eggNOGⁱ	KOG3804 Eukaryota ENOG4111K4J LUCA
GeneTreeⁱ	ENSGT00760000118996
HOGENOMⁱ	HOG000232188
HOVERGENⁱ	HBG074143
InParanoidⁱ	O70273
KOⁱ	K17102
OrthoDBⁱ	EOG091G0BE6
PhylomeDBⁱ	O70273
TreeFamⁱ	TF318679

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O70273
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000012350 Expressed in 154 organ(s), highest expression level in submandibular gland
CleanExⁱ	MM_EHF
ExpressionAtlasⁱ	O70273 baseline and differential
Genevisibleⁱ	O70273 MM

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR033071 EHF IPR000418 Ets_dom IPR003118 Pointed_dom IPR013761 SAM/pointed_sf IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
PANTHERⁱ	PTHR11849:SF171 PTHR11849:SF171, 1 hit
Pfamⁱ	View protein in Pfam PF00178 Ets, 1 hit PF02198 SAM_PNT, 1 hit
PRINTSⁱ	PR00454 ETSDOMAIN
SMARTⁱ	View protein in SMART SM00413 ETS, 1 hit SM00251 SAM_PNT, 1 hit
SUPFAMⁱ	SSF46785 SSF46785, 1 hit SSF47769 SSF47769, 1 hit
PROSITEⁱ	View protein in PROSITE PS50061 ETS_DOMAIN_3, 1 hit PS51433 PNT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	EHF_MOUSE
Accessionⁱ	O70273Primary (citable) accession number: O70273 Secondary accession number(s): Q922E8, Q922T6, Q99K12
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
	Last sequence update:	August 1, 1998
	Last modified:	November 7, 2018
	This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O70273 (EHF_MOUSE)

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ETS homologous factor

Ehf

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Golgi apparatus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ