UniProtKB - O70273 (EHF_MOUSE)
Protein
ETS homologous factor
Gene
Ehf
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes, and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter (By similarity).By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 207 – 289 | ETSPROSITE-ProRule annotationAdd BLAST | 83 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- cell differentiation Source: GO_Central
- epithelial cell differentiation Source: UniProtKB
- epithelial cell proliferation Source: InterPro
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- transcription by RNA polymerase II Source: InterPro
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: ETS homologous factorAlternative name(s): ETS domain-containing transcription factor |
Gene namesi | Name:EhfImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1270840, Ehf |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotationBy similarity
Golgi apparatus
- Golgi apparatus Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000257970 | 1 – 300 | ETS homologous factorAdd BLAST | 300 |
Proteomic databases
MaxQBi | O70273 |
PaxDbi | O70273 |
PeptideAtlasi | O70273 |
PRIDEi | O70273 |
ProteomicsDBi | 277567 [O70273-1] 277568 [O70273-2] 277569 [O70273-3] |
PTM databases
PhosphoSitePlusi | O70273 |
Expressioni
Tissue specificityi
Highly expressed in kidney and lung, weakly in skeletal muscle, heart, and liver, and not detected in brain, spleen or testis.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000012350, Expressed in secondary oocyte and 174 other tissues |
ExpressionAtlasi | O70273, baseline and differential |
Genevisiblei | O70273, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 199409, 1 interactor |
STRINGi | 10090.ENSMUSP00000087961 |
Miscellaneous databases
RNActi | O70273, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 115 | PNTPROSITE-ProRule annotationAdd BLAST | 87 |
Domaini
The PNT domain acts as a transcriptional activator.1 Publication
Sequence similaritiesi
Belongs to the ETS family.Sequence analysis
Phylogenomic databases
eggNOGi | KOG3804, Eukaryota |
GeneTreei | ENSGT00940000159310 |
InParanoidi | O70273 |
OrthoDBi | 837296at2759 |
PhylomeDBi | O70273 |
TreeFami | TF318679 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 1.10.150.50, 1 hit |
InterProi | View protein in InterPro IPR033071, EHF IPR000418, Ets_dom IPR003118, Pointed_dom IPR013761, SAM/pointed_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11849:SF171, PTHR11849:SF171, 1 hit |
Pfami | View protein in Pfam PF00178, Ets, 1 hit PF02198, SAM_PNT, 1 hit |
PRINTSi | PR00454, ETSDOMAIN |
SMARTi | View protein in SMART SM00413, ETS, 1 hit SM00251, SAM_PNT, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS50061, ETS_DOMAIN_3, 1 hit PS51433, PNT, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 11 Publication (identifier: O70273-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MILEGSGVMN LNPANNLLHQ QPAWTDSYPT CNVSSGFFGS QWHEIHPQYW
60 70 80 90 100
TKYQVWEWLQ HLLDTNQLDA SCIPFQEFDI SGEHLCSMSL QEFTRAAGSA
110 120 130 140 150
GQLLYSNLQH LKWNGQCSSD LFQSAHNVIV KTEQTDPSIM NTWKEENYLY
160 170 180 190 200
DPSYGSTVDL LDSKTFCRAQ ISMTTSSHLP VAESPDMKKE QDHPVKSHTK
210 220 230 240 250
KHNPRGTHLW EFIRDILLSP DKNPGLIKWE DRSEGIFRFL KSEAVAQLWG
260 270 280 290 300
KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2BIB5 | A2BIB5_MOUSE | ETS domain-containing transcription... | Ehf | 277 | Annotation score: | ||
B0S7M9 | B0S7M9_MOUSE | ETS homologous factor | Ehf | 242 | Annotation score: | ||
A2BIB6 | A2BIB6_MOUSE | ETS homologous factor | Ehf | 127 | Annotation score: | ||
A2BIB7 | A2BIB7_MOUSE | ETS homologous factor | Ehf | 181 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052191 | 79 – 93 | Missing in isoform 2. 1 PublicationAdd BLAST | 15 | |
Alternative sequenceiVSP_052192 | 200 – 211 | KKHNP…THLWE → YFIIELLLSESR in isoform 3. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_052193 | 212 – 300 | Missing in isoform 3. 1 PublicationAdd BLAST | 89 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF035527 mRNA Translation: AAC40119.1 BC005520 mRNA Translation: AAH05520.1 BC006789 mRNA Translation: AAH06789.1 BC008249 mRNA Translation: AAH08249.1 |
CCDSi | CCDS16475.1 [O70273-1] |
PIRi | JW0048 |
RefSeqi | NP_031940.1, NM_007914.3 [O70273-1] XP_006498760.1, XM_006498697.2 [O70273-1] XP_006498761.1, XM_006498698.3 [O70273-1] XP_006498762.1, XM_006498699.1 [O70273-1] XP_006498763.1, XM_006498700.3 [O70273-1] XP_006498764.1, XM_006498701.3 [O70273-1] XP_006498765.1, XM_006498702.3 [O70273-1] |
Genome annotation databases
Ensembli | ENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350 [O70273-1] ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350 [O70273-1] |
GeneIDi | 13661 |
KEGGi | mmu:13661 |
UCSCi | uc008lio.1, mouse [O70273-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF035527 mRNA Translation: AAC40119.1 BC005520 mRNA Translation: AAH05520.1 BC006789 mRNA Translation: AAH06789.1 BC008249 mRNA Translation: AAH08249.1 |
CCDSi | CCDS16475.1 [O70273-1] |
PIRi | JW0048 |
RefSeqi | NP_031940.1, NM_007914.3 [O70273-1] XP_006498760.1, XM_006498697.2 [O70273-1] XP_006498761.1, XM_006498698.3 [O70273-1] XP_006498762.1, XM_006498699.1 [O70273-1] XP_006498763.1, XM_006498700.3 [O70273-1] XP_006498764.1, XM_006498701.3 [O70273-1] XP_006498765.1, XM_006498702.3 [O70273-1] |
3D structure databases
SMRi | O70273 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199409, 1 interactor |
STRINGi | 10090.ENSMUSP00000087961 |
PTM databases
PhosphoSitePlusi | O70273 |
Proteomic databases
MaxQBi | O70273 |
PaxDbi | O70273 |
PeptideAtlasi | O70273 |
PRIDEi | O70273 |
ProteomicsDBi | 277567 [O70273-1] 277568 [O70273-2] 277569 [O70273-3] |
Protocols and materials databases
Antibodypediai | 13027, 132 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000090475; ENSMUSP00000087961; ENSMUSG00000012350 [O70273-1] ENSMUST00000111174; ENSMUSP00000106805; ENSMUSG00000012350 [O70273-1] |
GeneIDi | 13661 |
KEGGi | mmu:13661 |
UCSCi | uc008lio.1, mouse [O70273-1] |
Organism-specific databases
CTDi | 26298 |
MGIi | MGI:1270840, Ehf |
Phylogenomic databases
eggNOGi | KOG3804, Eukaryota |
GeneTreei | ENSGT00940000159310 |
InParanoidi | O70273 |
OrthoDBi | 837296at2759 |
PhylomeDBi | O70273 |
TreeFami | TF318679 |
Miscellaneous databases
BioGRID-ORCSi | 13661, 1 hit in 52 CRISPR screens |
ChiTaRSi | Ehf, mouse |
PROi | PR:O70273 |
RNActi | O70273, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000012350, Expressed in secondary oocyte and 174 other tissues |
ExpressionAtlasi | O70273, baseline and differential |
Genevisiblei | O70273, MM |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 1.10.150.50, 1 hit |
InterProi | View protein in InterPro IPR033071, EHF IPR000418, Ets_dom IPR003118, Pointed_dom IPR013761, SAM/pointed_sf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
PANTHERi | PTHR11849:SF171, PTHR11849:SF171, 1 hit |
Pfami | View protein in Pfam PF00178, Ets, 1 hit PF02198, SAM_PNT, 1 hit |
PRINTSi | PR00454, ETSDOMAIN |
SMARTi | View protein in SMART SM00413, ETS, 1 hit SM00251, SAM_PNT, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS50061, ETS_DOMAIN_3, 1 hit PS51433, PNT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EHF_MOUSE | |
Accessioni | O70273Primary (citable) accession number: O70273 Secondary accession number(s): Q922E8, Q922T6, Q99K12 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | August 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families