UniProtKB - O70239 (AXIN1_RAT)
Axin-1
Axin1
Functioni
GO - Molecular functioni
- armadillo repeat domain binding Source: BHF-UCL
- beta-catenin binding Source: BHF-UCL
- enzyme binding Source: RGD
- identical protein binding Source: IntAct
- I-SMAD binding Source: RGD
- molecular adaptor activity Source: RGD
- p53 binding Source: RGD
- protein C-terminus binding Source: RGD
- protein domain specific binding Source: RGD
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: BHF-UCL
- protein self-association Source: RGD
- R-SMAD binding Source: RGD
- signaling receptor binding Source: BHF-UCL
- signaling receptor complex adaptor activity Source: RGD
- SMAD binding Source: RGD
- ubiquitin protein ligase binding Source: RGD
GO - Biological processi
- activation of protein kinase activity Source: RGD
- adult walking behavior Source: RGD
- apoptotic process Source: UniProtKB-KW
- axial mesoderm development Source: RGD
- axial mesoderm formation Source: RGD
- canonical Wnt signaling pathway Source: RGD
- cellular protein-containing complex assembly Source: RGD
- cytoplasmic microtubule organization Source: RGD
- dorsal/ventral axis specification Source: RGD
- dorsal/ventral pattern formation Source: RGD
- embryonic skeletal joint morphogenesis Source: RGD
- erythrocyte homeostasis Source: RGD
- genetic imprinting Source: RGD
- head development Source: RGD
- hemoglobin metabolic process Source: RGD
- in utero embryonic development Source: RGD
- negative regulation of canonical Wnt signaling pathway Source: RGD
- negative regulation of fat cell differentiation Source: RGD
- negative regulation of gene expression Source: RGD
- negative regulation of protein metabolic process Source: RGD
- negative regulation of transcription elongation from RNA polymerase II promoter Source: RGD
- negative regulation of Wnt signaling pathway Source: RGD
- nervous system process Source: RGD
- nucleocytoplasmic transport Source: RGD
- positive regulation of JNK cascade Source: UniProtKB
- positive regulation of JUN kinase activity Source: RGD
- positive regulation of peptidyl-serine phosphorylation Source: RGD
- positive regulation of peptidyl-threonine phosphorylation Source: RGD
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: RGD
- positive regulation of protein catabolic process Source: RGD
- positive regulation of protein kinase activity Source: RGD
- positive regulation of protein phosphorylation Source: RGD
- positive regulation of protein ubiquitination Source: RGD
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transforming growth factor beta receptor signaling pathway Source: RGD
- positive regulation of ubiquitin-dependent protein catabolic process Source: BHF-UCL
- positive regulation of ubiquitin-protein transferase activity Source: RGD
- post-anal tail morphogenesis Source: RGD
- protein catabolic process Source: RGD
- protein polyubiquitination Source: RGD
- regulation of canonical Wnt signaling pathway Source: RGD
- regulation of protein phosphorylation Source: RGD
- sensory perception of sound Source: RGD
- Wnt signaling pathway Source: RGD
Keywordsi
Molecular function | Developmental protein |
Biological process | Apoptosis, Wnt signaling pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Axin-1Alternative name(s): Axis inhibition protein 1 Short name: rAxin |
Gene namesi | Name:Axin1 Synonyms:Axin |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620859, Axin1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- Nucleus By similarity
Plasma membrane
- Cell membrane 1 Publication
Other locations
- Membrane By similarity
Note: On UV irradiation, translocates to the nucleus and colocalizes with DAAX (By similarity). MACF1 is required for its translocation to cell membrane (By similarity).By similarity
Cytoskeleton
- microtubule cytoskeleton Source: RGD
Nucleus
- nucleus Source: RGD
Plasma Membrane
- lateral plasma membrane Source: RGD
- plasma membrane Source: UniProtKB
Other locations
- beta-catenin destruction complex Source: BHF-UCL
- cell cortex Source: RGD
- cell periphery Source: RGD
- cytoplasm Source: RGD
- cytoplasmic vesicle Source: BHF-UCL
- perinuclear region of cytoplasm Source: RGD
- postsynaptic density Source: RGD
- protein-containing complex Source: RGD
- Wnt signalosome Source: RGD
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000220890 | 1 – 827 | Axin-1Add BLAST | 827 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 75 | Phosphoserine; by CK1By similarity | 1 | |
Modified residuei | 77 | Phosphoserine; by CK1By similarity | 1 | |
Modified residuei | 217 | Phosphoserine; by CK1By similarity | 1 | |
Modified residuei | 468 | Phosphoserine; by CK1By similarity | 1 | |
Modified residuei | 480 | Phosphothreonine; by GSK3-betaBy similarity | 1 | |
Modified residuei | 485 | Phosphoserine; by GSK3-betaBy similarity | 1 | |
Modified residuei | 492 | PhosphoserineBy similarity | 1 | |
Modified residuei | 509 | PhosphoserineBy similarity | 1 | |
Modified residuei | 578 | PhosphoserineBy similarity | 1 | |
Cross-linki | 822 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 825 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
Keywords - PTMi
ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | O70239 |
PRIDEi | O70239 |
PTM databases
iPTMneti | O70239 |
PhosphoSitePlusi | O70239 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with ZBED3; the interaction is direct, enhanced by protein kinase GSK3B and casein kinase CSNK1E activities and decreases GSK3B-induced beta-catenin serine and threonine phosphorylations (By similarity).
Component of the AXIN1-HIPK2-TP53 complex (By similarity).
Interacts directly in the complex with TP53 and HIPK2 (By similarity).
Interacts with DAXX; the interaction stimulates the interaction of DAXX with TP53, stimulates 'Ser-46' phosphorylation of TP53 and induces cell death on UV irradiation (By similarity). Also binds ANKRD6, PIAS1, PIAS2, PIAS4, MAP3K1, MAP3K4, SUMO1, SMAD6, SMAD7 and RNF111 (By similarity).
Interacts with DIXDC1; the interaction prevents interaction with MAP3K1 (By similarity).
Interacts with MDFI; the interaction decreases AXIN1-mediated JUN N-terminal kinase (JNK) activation (By similarity).
Interacts with MDFIC; the interaction inhibits beta-cateninin-mediated signaling and AXIN1-mediated JUN N-terminal kinase (JNK) activation (By similarity).
Interacts with LRP5 (via its phosphorylated PPPSP motifs); the interaction is stimulated by WNT1 and GSK3B and activates beta-catenin signaling (By similarity).
Interacts (via the C-terminal) with PPP1CA; the interaction dephosphorylates AXIN1 and regulates interaction with GSK3B (By similarity).
Interacts with PPP2CA; the interaction dephosphorylates AXIN1 (By similarity).
Component of the beta-catenin destruction complex, containing at least, CTNNB1, an axin and GSK3B, that regulates CTNNB1 protein levels through phosphorylation and ubiquitination (By similarity).
Interacts with CTNNB1 (via the armadillo repeats 2-7) (By similarity).
Interacts with GSK3B; the interaction hyperphosphorylates CTNNB1 leading to its ubiquitination and destruction (PubMed:9482734, PubMed:16815997).
Interacts with MACF1 (PubMed:16815997).
Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B (PubMed:16815997).
Interacts with TNKS (By similarity).
Interacts with DAB2; the interaction is mutually exclusive with the AXIN1:PPP1CA interaction (By similarity).
Interacts with WDR26 (By similarity).
Interacts with GID8 (By similarity).
By similarity2 PublicationsBinary interactionsi
Hide detailsO70239
GO - Molecular functioni
- armadillo repeat domain binding Source: BHF-UCL
- beta-catenin binding Source: BHF-UCL
- enzyme binding Source: RGD
- identical protein binding Source: IntAct
- I-SMAD binding Source: RGD
- p53 binding Source: RGD
- protein C-terminus binding Source: RGD
- protein domain specific binding Source: RGD
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: BHF-UCL
- protein self-association Source: RGD
- R-SMAD binding Source: RGD
- signaling receptor binding Source: BHF-UCL
- SMAD binding Source: RGD
- ubiquitin protein ligase binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249455, 9 interactors |
CORUMi | O70239 |
DIPi | DIP-29400N |
ELMi | O70239 |
IntActi | O70239, 10 interactors |
MINTi | O70239 |
STRINGi | 10116.ENSRNOP00000027705 |
Structurei
Secondary structure
3D structure databases
SMRi | O70239 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O70239 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 88 – 211 | RGSPROSITE-ProRule annotationAdd BLAST | 124 | |
Domaini | 745 – 827 | DIXPROSITE-ProRule annotationAdd BLAST | 83 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 209 – 338 | Interaction with TP53By similarityAdd BLAST | 130 | |
Regioni | 348 – 432 | Interaction with GSK3B1 PublicationAdd BLAST | 85 | |
Regioni | 433 – 501 | Interaction with CTNNB1Add BLAST | 69 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 20 – 29 | Tankyrase-binding motif | 10 |
Domaini
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
InParanoidi | O70239 |
Family and domain databases
CDDi | cd11582, Axin_TNKS_binding, 1 hit |
Gene3Di | 1.10.196.10, 2 hits 3.10.20.380, 1 hit |
IDEALi | IID50195 |
InterProi | View protein in InterPro IPR043581, Axin-like IPR029797, AXIN1 IPR014936, Axin_b-cat-bd IPR032101, Axin_TNKS-bd IPR001158, DIX IPR038207, DIX_dom_sf IPR016137, RGS IPR036305, RGS_sf IPR024066, RGS_subdom1/3 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR46102, PTHR46102, 1 hit PTHR46102:SF3, PTHR46102:SF3, 1 hit |
Pfami | View protein in Pfam PF16646, AXIN1_TNKS_BD, 1 hit PF08833, Axin_b-cat_bind, 1 hit PF00778, DIX, 1 hit PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00021, DAX, 1 hit SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50841, DIX, 1 hit PS50132, RGS, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNVQEQGFPL DLGASFTEDA PRPPVPGEEG ELVSTDSRPV NHSFCSGKGT
60 70 80 90 100
SIKSETSTAT PRRSDLDLGY EPEGSASPTP PYLRWAESLH SLLDDQDGIS
110 120 130 140 150
LFRTFLKQEG CADLLDFWFA CSGFRKLEPC DSNEEKRLKL ARAIYRKYIL
160 170 180 190 200
DSNGIVSRQT KPATKSFIKD CVMKQQIDPA MFDQAQTEIQ STMEENTYPS
210 220 230 240 250
FLKSDIYLEY TRTGSESPKV CSDQSSGSGT GKGMSGYLPT LNEDEEWKCD
260 270 280 290 300
QDADEDDGRD SVPPSRLTQK LLLETAAPRA PSSRRYNEGR ELRYGSWREP
310 320 330 340 350
VNPYYVNSGY ALAPATSAND SEQQSLSSDA DTLSLTDSSV DGIPPYRIRK
360 370 380 390 400
QHRREMQESV QVNGRVPLPH IPRTYRMPKE IRVEPQKFAE ELIHRLEAVQ
410 420 430 440 450
RTREAEEKLE ERLKRVRMEE EGEDGEMPSG PMASHKLPSV PAWHHFPPRY
460 470 480 490 500
VDMGCSGLRD AHEENPESIL DEHVQRVMRT PGCQSPGPGH RSPDSGHVAK
510 520 530 540 550
TAVLGGTASG HGKHAPKLGL KLDSAGLHHH RHVHHHVHHN SARPKEQMEA
560 570 580 590 600
EAARRVQSSF SWGPETHGHA KPRSYSESTG TNPSAGDLAF GGKASAPSKR
610 620 630 640 650
NTKKAESGKN ASAEVPSTTE DAEKNQKIMQ WIIEGEKEIS RHRKAGHGSS
660 670 680 690 700
GMRKQQAHES SRPLSIERPG AVHPWVSAQL RNSVQPSHLF IQDPTMPPNP
710 720 730 740 750
APNPLTQLEE ARRRLEEEEK RANKLPSKQR TKSQRKAGGG SAPPCDSIVV
760 770 780 790 800
AYYFCGEPIP YRTLVRGRAV TLGQFKELLT KKGSYRYYFK KVSDEFDCGV
810 820
VFEEVREDEA ILPVFEEKII GKVEKVD
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017756 mRNA Translation: AAC40066.1 Different initiation. |
PIRi | T08422 |
RefSeqi | NP_077381.1, NM_024405.1 |
Genome annotation databases
GeneIDi | 79257 |
KEGGi | rno:79257 |
UCSCi | RGD:620859, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017756 mRNA Translation: AAC40066.1 Different initiation. |
PIRi | T08422 |
RefSeqi | NP_077381.1, NM_024405.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WSP | X-ray | 2.90 | A/B/C | 744-827 | [»] | |
2D5G | X-ray | 3.20 | A/B/C/D/E/F | 743-827 | [»] | |
SMRi | O70239 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 249455, 9 interactors |
CORUMi | O70239 |
DIPi | DIP-29400N |
ELMi | O70239 |
IntActi | O70239, 10 interactors |
MINTi | O70239 |
STRINGi | 10116.ENSRNOP00000027705 |
PTM databases
iPTMneti | O70239 |
PhosphoSitePlusi | O70239 |
Proteomic databases
PaxDbi | O70239 |
PRIDEi | O70239 |
Genome annotation databases
GeneIDi | 79257 |
KEGGi | rno:79257 |
UCSCi | RGD:620859, rat |
Organism-specific databases
CTDi | 8312 |
RGDi | 620859, Axin1 |
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
InParanoidi | O70239 |
Miscellaneous databases
EvolutionaryTracei | O70239 |
PROi | PR:O70239 |
Family and domain databases
CDDi | cd11582, Axin_TNKS_binding, 1 hit |
Gene3Di | 1.10.196.10, 2 hits 3.10.20.380, 1 hit |
IDEALi | IID50195 |
InterProi | View protein in InterPro IPR043581, Axin-like IPR029797, AXIN1 IPR014936, Axin_b-cat-bd IPR032101, Axin_TNKS-bd IPR001158, DIX IPR038207, DIX_dom_sf IPR016137, RGS IPR036305, RGS_sf IPR024066, RGS_subdom1/3 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR46102, PTHR46102, 1 hit PTHR46102:SF3, PTHR46102:SF3, 1 hit |
Pfami | View protein in Pfam PF16646, AXIN1_TNKS_BD, 1 hit PF08833, Axin_b-cat_bind, 1 hit PF00778, DIX, 1 hit PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00021, DAX, 1 hit SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50841, DIX, 1 hit PS50132, RGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AXIN1_RAT | |
Accessioni | O70239Primary (citable) accession number: O70239 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 10, 2002 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references