UniProtKB - O70201 (BIRC5_MOUSE)
Baculoviral IAP repeat-containing protein 5
Birc5
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 57 | Zinc 1 | 1 | |
Metal bindingi | 60 | Zinc 1 | 1 | |
Metal bindingi | 76 | Zinc 2 | 1 | |
Metal bindingi | 77 | Zinc 1 | 1 | |
Metal bindingi | 80 | Zinc 2 | 1 | |
Metal bindingi | 84 | Zinc 1 | 1 |
GO - Molecular functioni
- chaperone binding Source: MGI
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- microtubule binding Source: UniProtKB
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: UniProtKB
- small GTPase binding Source: MGI
- tubulin binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- cell division Source: UniProtKB
- chromosome segregation Source: UniProtKB-KW
- establishment of chromosome localization Source: UniProtKB
- G2/M transition of mitotic cell cycle Source: UniProtKB
- microtubule cytoskeleton organization Source: MGI
- mitotic cytokinesis Source: UniProtKB
- mitotic spindle assembly checkpoint Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- negative regulation of neuron apoptotic process Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of cell cycle Source: MGI
- positive regulation of exit from mitosis Source: UniProtKB
- positive regulation of mitotic cell cycle Source: UniProtKB
- protein-containing complex localization Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of cell cycle Source: GO_Central
- regulation of insulin secretion involved in cellular response to glucose stimulus Source: MGI
- regulation of mitotic cell cycle Source: MGI
- regulation of type B pancreatic cell proliferation Source: MGI
Keywordsi
Molecular function | Protease inhibitor, Repressor, Thiol protease inhibitor |
Biological process | Apoptosis, Cell cycle, Cell division, Chromosome partition, Mitosis, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-4615885, SUMOylation of DNA replication proteins R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-8951664, Neddylation R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Protein family/group databases
MEROPSi | I32.005 |
Names & Taxonomyi
Protein namesi | Recommended name: Baculoviral IAP repeat-containing protein 5Alternative name(s): Apoptosis inhibitor 4 Apoptosis inhibitor survivin TIAP |
Gene namesi | Name:Birc5 Synonyms:Api4, Iap4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1203517, Birc5 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- Nucleus By similarity
Cytoskeleton
- spindle By similarity
Other locations
- Chromosome By similarity
- centromere By similarity
- kinetochore By similarity
- Midbody By similarity
Note: Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase and a the midbody during telophase and cytokinesis. Accumulates in the nucleus upon treatment with leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor (By similarity). Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (By similarity).By similarity
Cytoskeleton
- centriole Source: UniProtKB
- chromosome passenger complex Source: UniProtKB
- cytoplasmic microtubule Source: UniProtKB
- interphase microtubule organizing center Source: UniProtKB
- spindle microtubule Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nuclear chromosome Source: MGI
- nucleus Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: MGI
Other locations
- chromosome, centromeric region Source: UniProtKB
- condensed chromosome kinetochore Source: UniProtKB
- cytoplasm Source: MGI
- midbody Source: UniProtKB
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122357 | 1 – 140 | Baculoviral IAP repeat-containing protein 5Add BLAST | 140 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 23 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 34 | Phosphothreonine; by CDK1 and CDK151 Publication | 1 | |
Modified residuei | 48 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 90 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 110 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 112 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 115 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 117 | Phosphothreonine; by AURKBBy similarity | 1 | |
Modified residuei | 129 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O70201 |
PaxDbi | O70201 |
PeptideAtlasi | O70201 |
PRIDEi | O70201 |
ProteomicsDBi | 273492 [O70201-1] 273493 [O70201-2] 273494 [O70201-3] |
PTM databases
iPTMneti | O70201 |
PhosphoSitePlusi | O70201 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000017716, Expressed in primary oocyte and 274 other tissues |
ExpressionAtlasi | O70201, baseline and differential |
Genevisiblei | O70201, MM |
Interactioni
Subunit structurei
Monomer or homodimer. Exists as a homodimer in the apo state and as a monomer in the CPC-bound state. The monomer protects cells against apoptosis more efficiently than the dimer. Only the dimeric form is capable of enhancing tubulin stability in cells. When phosphorylated, interacts with LAMTOR5/HBXIP; the resulting complex binds pro-CASP9, as well as active CASP9, but much less efficiently.
Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex forms a triple-helix bundle-based subcomplex with INCENP and CDCA8.
Interacts with JTB.
Interacts (via BIR domain) with histone H3 phosphorylated at 'Thr-3' (H3pT3).
Interacts with EVI5.
Interacts with GTP-bound RAN in both the S and M phases of the cell cycle.
Interacts with USP9X.
Interacts with tubulin.
Interacts with BIRC2/c-IAP1. The acetylated form at Lys-129 interacts with STAT3. The monomeric form deacetylated at Lys-129 interacts with XPO1/CRM1. The monomeric form interacts with XIAP/BIRC4. Both the dimeric and monomeric form can interact with DIABLO/SMAC.
Interacts with BIRC6/bruce.
Interacts with FBXL7; this interaction facilitates the polyubiquitination and subsequent proteasomal degradation of BIRC5 by the SCF(FBXL7) E3 ubiquitin-protein ligase complex (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 126 | Interaction with FBXL7By similarity | 1 |
GO - Molecular functioni
- chaperone binding Source: MGI
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- microtubule binding Source: UniProtKB
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: UniProtKB
- small GTPase binding Source: MGI
- tubulin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 198150, 2 interactors |
ComplexPortali | CPX-113, Survivin homodimer complex CPX-119, Chromosomal passenger complex |
STRINGi | 10090.ENSMUSP00000079124 |
Miscellaneous databases
RNActi | O70201, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O70201 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O70201 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 18 – 88 | BIRAdd BLAST | 71 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1101, Eukaryota |
GeneTreei | ENSGT00510000047537 |
HOGENOMi | CLU_016347_0_1_1 |
InParanoidi | O70201 |
OMAi | TKMYFYE |
PhylomeDBi | O70201 |
TreeFami | TF342652 |
Family and domain databases
CDDi | cd00022, BIR, 1 hit |
InterProi | View protein in InterPro IPR001370, BIR_rpt |
Pfami | View protein in Pfam PF00653, BIR, 1 hit |
SMARTi | View protein in SMART SM00238, BIR, 1 hit |
PROSITEi | View protein in PROSITE PS50143, BIR_REPEAT_2, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGAPALPQIW QLYLKNYRIA TFKNWPFLED CACTPERMAE AGFIHCPTEN
60 70 80 90 100
EPDLAQCFFC FKELEGWEPD DNPIEEHRKH SPGCAFLTVK KQMEELTVSE
110 120 130 140
FLKLDRQRAK NKIAKETNNK QKEFEETAKT TRQSIEQLAA
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002455 | 38 – 40 | MAE → RGA in isoform 3. Curated | 3 | |
Alternative sequenceiVSP_002456 | 41 – 140 | Missing in isoform 3. CuratedAdd BLAST | 100 | |
Alternative sequenceiVSP_002457 | 114 – 140 | AKETN…EQLAA → VCMIENKD in isoform 2. 1 PublicationAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF077349 mRNA Translation: AAD34225.1 AB013819 mRNA Translation: BAA28266.1 AF115517 Genomic DNA Translation: AAD26199.1 AF115517 Genomic DNA Translation: AAD26200.1 AF115517 Genomic DNA Translation: AAD26201.1 BC004702 mRNA Translation: AAH04702.1 |
CCDSi | CCDS25694.1 [O70201-1] CCDS25695.1 [O70201-2] |
RefSeqi | NP_001012273.1, NM_001012273.1 [O70201-2] NP_033819.1, NM_009689.2 [O70201-1] |
Genome annotation databases
Ensembli | ENSMUST00000081387; ENSMUSP00000079124; ENSMUSG00000017716 [O70201-1] ENSMUST00000093906; ENSMUSP00000091433; ENSMUSG00000017716 [O70201-2] |
GeneIDi | 11799 |
KEGGi | mmu:11799 |
UCSCi | uc007mod.1, mouse [O70201-1] uc007moe.1, mouse [O70201-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF077349 mRNA Translation: AAD34225.1 AB013819 mRNA Translation: BAA28266.1 AF115517 Genomic DNA Translation: AAD26199.1 AF115517 Genomic DNA Translation: AAD26200.1 AF115517 Genomic DNA Translation: AAD26201.1 BC004702 mRNA Translation: AAH04702.1 |
CCDSi | CCDS25694.1 [O70201-1] CCDS25695.1 [O70201-2] |
RefSeqi | NP_001012273.1, NM_001012273.1 [O70201-2] NP_033819.1, NM_009689.2 [O70201-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1M4M | X-ray | 2.80 | A | 1-140 | [»] | |
SMRi | O70201 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198150, 2 interactors |
ComplexPortali | CPX-113, Survivin homodimer complex CPX-119, Chromosomal passenger complex |
STRINGi | 10090.ENSMUSP00000079124 |
Protein family/group databases
MEROPSi | I32.005 |
PTM databases
iPTMneti | O70201 |
PhosphoSitePlusi | O70201 |
Proteomic databases
EPDi | O70201 |
PaxDbi | O70201 |
PeptideAtlasi | O70201 |
PRIDEi | O70201 |
ProteomicsDBi | 273492 [O70201-1] 273493 [O70201-2] 273494 [O70201-3] |
Protocols and materials databases
Antibodypediai | 1073, 1750 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000081387; ENSMUSP00000079124; ENSMUSG00000017716 [O70201-1] ENSMUST00000093906; ENSMUSP00000091433; ENSMUSG00000017716 [O70201-2] |
GeneIDi | 11799 |
KEGGi | mmu:11799 |
UCSCi | uc007mod.1, mouse [O70201-1] uc007moe.1, mouse [O70201-2] |
Organism-specific databases
CTDi | 332 |
MGIi | MGI:1203517, Birc5 |
Phylogenomic databases
eggNOGi | KOG1101, Eukaryota |
GeneTreei | ENSGT00510000047537 |
HOGENOMi | CLU_016347_0_1_1 |
InParanoidi | O70201 |
OMAi | TKMYFYE |
PhylomeDBi | O70201 |
TreeFami | TF342652 |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-4615885, SUMOylation of DNA replication proteins R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-8951664, Neddylation R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
BioGRID-ORCSi | 11799, 20 hits in 49 CRISPR screens |
ChiTaRSi | Birc5, mouse |
EvolutionaryTracei | O70201 |
PROi | PR:O70201 |
RNActi | O70201, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000017716, Expressed in primary oocyte and 274 other tissues |
ExpressionAtlasi | O70201, baseline and differential |
Genevisiblei | O70201, MM |
Family and domain databases
CDDi | cd00022, BIR, 1 hit |
InterProi | View protein in InterPro IPR001370, BIR_rpt |
Pfami | View protein in Pfam PF00653, BIR, 1 hit |
SMARTi | View protein in SMART SM00238, BIR, 1 hit |
PROSITEi | View protein in PROSITE PS50143, BIR_REPEAT_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BIRC5_MOUSE | |
Accessioni | O70201Primary (citable) accession number: O70201 Secondary accession number(s): Q923F7, Q9WU53, Q9WU54 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | August 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families