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Protein

Eukaryotic translation initiation factor 3 subunit D

Gene

Eif3d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs.UniRule annotation

GO - Molecular functioni

  • mRNA cap binding Source: UniProtKB
  • RNA binding Source: MGI
  • translation initiation factor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit DUniRule annotation
Short name:
eIF3dUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 7UniRule annotation
eIF-3-zetaUniRule annotation
eIF3 p66
Gene namesi
Name:Eif3d
Synonyms:Eif3s7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1933181 Eif3d

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001235211 – 548Eukaryotic translation initiation factor 3 subunit DAdd BLAST548

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-acetyllysineCombined sources1
Modified residuei161PhosphoserineBy similarity1
Modified residuei528PhosphoserineCombined sources1
Modified residuei529PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO70194
MaxQBiO70194
PaxDbiO70194
PeptideAtlasiO70194
PRIDEiO70194

PTM databases

iPTMnetiO70194
PhosphoSitePlusiO70194
SwissPalmiO70194

Expressioni

Gene expression databases

BgeeiENSMUSG00000016554 Expressed in 294 organ(s), highest expression level in midbrain
GenevisibleiO70194 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi207740, 2 interactors
IntActiO70194, 8 interactors
MINTiO70194
STRINGi10090.ENSMUSP00000098053

Structurei

3D structure databases

ProteinModelPortaliO70194
SMRiO70194
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni285 – 299RNA gateBy similarityAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi530 – 547Asp/Glu-richAdd BLAST18

Domaini

The RNA gate region regulates mRNA cap recognition to prevent promiscuous mRNA-binding before assembly of eif3d into the full eukaryotic translation initiation factor 3 (eIF-3) complex.UniRule annotation

Sequence similaritiesi

Belongs to the eIF-3 subunit D family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2479 Eukaryota
ENOG410XP1E LUCA
GeneTreeiENSGT00390000002667
HOGENOMiHOG000241593
HOVERGENiHBG000849
InParanoidiO70194
KOiK03251
OMAiDRIFHTV
OrthoDBiEOG091G07IK
PhylomeDBiO70194
TreeFamiTF101519

Family and domain databases

HAMAPiMF_03003 eIF3d, 1 hit
InterProiView protein in InterPro
IPR007783 eIF3d
PANTHERiPTHR12399 PTHR12399, 1 hit
PfamiView protein in Pfam
PF05091 eIF-3_zeta, 1 hit
PIRSFiPIRSF016281 EIF-3_zeta, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O70194-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKFMTPVIQ DNPSGWGPCA VPEQFRDMPY QPFSKGDRLG KVADWTGATY
60 70 80 90 100
QDKRYTNKYS SQFGGGSQYA YFHEEDETSF QLVDTARTQK TAYQRNRMRF
110 120 130 140 150
AQRNLRRDKD RRNMVQFNLQ TLPKSAKQKE RERIRLQKKF QKQFGVRQKW
160 170 180 190 200
DQKSQKPRDS SVEVRSDWEV KEEMDFPQLM KMRYLEVSEP QDIECCGALE
210 220 230 240 250
YYDKAFDRIT TRSEKPLRSI KRIFHTVTTT DDPVIRKLAK TQGNVFATDA
260 270 280 290 300
ILATLMSCTR SVYSWDIVVQ RVGSKLFFDK RDNSDFDLLT VSETANEPPQ
310 320 330 340 350
DEGNSFNSPR NLAMEATYIN HNFSQQCLRM GRERYNFPNP NPFVEDDMDK
360 370 380 390 400
NEIASVAYRY RRWKLGDDID LIVRCEHDGV MTGANGEVSF INIKTLNEWD
410 420 430 440 450
SRHCNGVDWR QKLDSQRGAV IATELKNNSY KLARWTCCAL LAGSEYLKLG
460 470 480 490 500
YVSRYHVKDS SRHVILGTQQ FKPNEFASQI NLSVENAWGI LRCVIDICMK
510 520 530 540
LEEGKYLILK DPNKQVIRVY SLPDGTFSSE EDEEDEEEEE EEEEEEET
Length:548
Mass (Da):63,989
Last modified:January 15, 2008 - v2
Checksum:iC23CA1743270DECD
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2R8VK20A0A2R8VK20_MOUSE
Eukaryotic translation initiation f...
Eif3d
169Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198 – 219ALEYY…KPLRS → PWSTTTKPLTASPQGVRSPC D in BAA25327 (Ref. 1) CuratedAdd BLAST22
Sequence conflicti281R → G in BAC32270 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012580 mRNA Translation: BAA25327.1
AK045228 mRNA Translation: BAC32270.1
AK049767 mRNA Translation: BAC33910.1
AK145689 mRNA Translation: BAE26591.1
AK168256 mRNA Translation: BAE40204.1
BC089020 mRNA Translation: AAH89020.1
CCDSiCCDS37130.1
RefSeqiNP_061219.2, NM_018749.2
XP_006521255.1, XM_006521192.1
UniGeneiMm.3955
Mm.475286

Genome annotation databases

EnsembliENSMUST00000100484; ENSMUSP00000098053; ENSMUSG00000016554
GeneIDi55944
KEGGimmu:55944
UCSCiuc007wom.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012580 mRNA Translation: BAA25327.1
AK045228 mRNA Translation: BAC32270.1
AK049767 mRNA Translation: BAC33910.1
AK145689 mRNA Translation: BAE26591.1
AK168256 mRNA Translation: BAE40204.1
BC089020 mRNA Translation: AAH89020.1
CCDSiCCDS37130.1
RefSeqiNP_061219.2, NM_018749.2
XP_006521255.1, XM_006521192.1
UniGeneiMm.3955
Mm.475286

3D structure databases

ProteinModelPortaliO70194
SMRiO70194
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207740, 2 interactors
IntActiO70194, 8 interactors
MINTiO70194
STRINGi10090.ENSMUSP00000098053

PTM databases

iPTMnetiO70194
PhosphoSitePlusiO70194
SwissPalmiO70194

Proteomic databases

EPDiO70194
MaxQBiO70194
PaxDbiO70194
PeptideAtlasiO70194
PRIDEiO70194

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100484; ENSMUSP00000098053; ENSMUSG00000016554
GeneIDi55944
KEGGimmu:55944
UCSCiuc007wom.1 mouse

Organism-specific databases

CTDi8664
MGIiMGI:1933181 Eif3d

Phylogenomic databases

eggNOGiKOG2479 Eukaryota
ENOG410XP1E LUCA
GeneTreeiENSGT00390000002667
HOGENOMiHOG000241593
HOVERGENiHBG000849
InParanoidiO70194
KOiK03251
OMAiDRIFHTV
OrthoDBiEOG091G07IK
PhylomeDBiO70194
TreeFamiTF101519

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Miscellaneous databases

ChiTaRSiEif3d mouse
PROiPR:O70194
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016554 Expressed in 294 organ(s), highest expression level in midbrain
GenevisibleiO70194 MM

Family and domain databases

HAMAPiMF_03003 eIF3d, 1 hit
InterProiView protein in InterPro
IPR007783 eIF3d
PANTHERiPTHR12399 PTHR12399, 1 hit
PfamiView protein in Pfam
PF05091 eIF-3_zeta, 1 hit
PIRSFiPIRSF016281 EIF-3_zeta, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3D_MOUSE
AccessioniPrimary (citable) accession number: O70194
Secondary accession number(s): Q8BLH0, Q8BWW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 15, 2008
Last modified: November 7, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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