UniProtKB - O70191 (ATF5_MOUSE)
Cyclic AMP-dependent transcription factor ATF-5
Atf5
Functioni
Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (By similarity).
Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation (PubMed:22095825).
Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes (PubMed:23090999).
May be involved in osteogenic differentiation. Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (By similarity).
Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent (By similarity) (PubMed:12130540).
Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver. In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase. May act as a negative regulator of IL1B transduction pathway in liver. Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members. Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation. Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (By similarity).
By similarity3 PublicationsGO - Molecular functioni
- chromatin binding Source: UniProtKB
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: UniProtKB
- heat shock protein binding Source: MGI
- kinase binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
- transcription cis-regulatory region binding Source: UniProtKB
- tubulin binding Source: MGI
GO - Biological processi
- cerebellar granule cell precursor proliferation Source: UniProtKB
- circadian rhythm Source: UniProtKB
- fat cell differentiation Source: UniProtKB
- multicellular organism growth Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of astrocyte differentiation Source: MGI
- negative regulation of cell cycle G2/M phase transition Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of neurogenesis Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- olfactory bulb interneuron development Source: MGI
- olfactory bulb interneuron differentiation Source: MGI
- olfactory lobe development Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- post-embryonic development Source: MGI
- regulation of centrosome cycle Source: UniProtKB
- regulation of gene expression Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclic AMP-dependent transcription factor ATF-5Short name: cAMP-dependent transcription factor ATF-5 Alternative name(s): Activating transcription factor 5-alpha/beta BZIP protein ATF7 NAP1 NRIF3-associated protein Transcription factor ATFx Transcription factor-like protein ODA-10 |
Gene namesi | Name:Atf5 Synonyms:Atfx, Nap1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2141857, Atf5 |
VEuPathDBi | HostDB:ENSMUSG00000038539 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytoskeleton
- centrosome By similarity
Nucleus
- Nucleus 1 Publication
Note: Actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, translocates from the centrosome to the midbody.By similarity
Cytoskeleton
- centrosome Source: UniProtKB
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: MGI
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 29 | K → Q: Enhances interaction with CEBPB. 1 Publication | 1 | |
Mutagenesisi | 29 | K → R: Decreases acetylation levels and interaction with CEBPB. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076587 | 1 – 283 | Cyclic AMP-dependent transcription factor ATF-5Add BLAST | 283 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 29 | N6-acetyllysine; by EP3001 Publication | 1 | |
Modified residuei | 257 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | O70191 |
PRIDEi | O70191 |
PTM databases
iPTMneti | O70191 |
PhosphoSitePlusi | O70191 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000038539, Expressed in olfactory epithelium and 316 other tissues |
Genevisiblei | O70191, MM |
Interactioni
Subunit structurei
Binds DNA as a dimer.
Interacts with PTP4A1/PRL-1 (By similarity).
Interacts with CCND3, but not with CCND1 or CCND2.
Interacts with HSPA1A or HSPA1B; the interaction protects ATF5 from degradation via proteasome-dependent and caspase-dependent processes.
Interacts (via C-terminal region) with NPM1 (via C-terminal region); the interaction leads to loss of association between HSPA1A or HSPA1B and ATF5 and promotes ATF5 degradation via proteasome-dependent and caspase-dependent processes.
Interacts with NLK; the interaction stabilizes ATF5 at the protein level in a kinase-independent manner.
Interacts with alpha-tubulin, gamma-tubulin members TUBGCP2 and TUBGCP4, PCNT; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome (By similarity).
Interacts with CEBPB and EP300; EP300 is required for ATF5 and CEBPB interaction and DNA binding (PubMed:24216764).
By similarity2 PublicationsGO - Molecular functioni
- heat shock protein binding Source: MGI
- kinase binding Source: MGI
- tubulin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 223346, 2 interactors |
IntActi | O70191, 1 interactor |
STRINGi | 10090.ENSMUSP00000047771 |
Miscellaneous databases
RNActi | O70191, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 209 – 272 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 21 | Required for protein stabilization induced by IL1BBy similarityAdd BLAST | 21 | |
Regioni | 118 – 154 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 119 – 218 | Interaction with PTP4A11 PublicationAdd BLAST | 100 | |
Regioni | 168 – 241 | DisorderedSequence analysisAdd BLAST | 74 | |
Regioni | 211 – 231 | Basic motifPROSITE-ProRule annotationAdd BLAST | 21 | |
Regioni | 237 – 251 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 15 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 120 – 151 | Pro residuesSequence analysisAdd BLAST | 32 | |
Compositional biasi | 181 – 202 | Pro residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 208 – 241 | Basic and acidic residuesSequence analysisAdd BLAST | 34 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4571, Eukaryota |
GeneTreei | ENSGT00530000063801 |
HOGENOMi | CLU_083640_0_0_1 |
InParanoidi | O70191 |
OMAi | KIPGHYE |
OrthoDBi | 1524842at2759 |
PhylomeDBi | O70191 |
TreeFami | TF316136 |
Family and domain databases
InterProi | View protein in InterPro IPR029855, ATF5 IPR004827, bZIP IPR046347, bZIP_sf |
PANTHERi | PTHR13044:SF3, PTHR13044:SF3, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSLLATLGLE LDRALLPASG LGWLVDYGKL PLAPAPLGPY EVLGGALEGG
60 70 80 90 100
LPGGGEPLAG DGFSDWMTER VDFTALLPLE APLPPGTLPP PSPAPPDLEA
110 120 130 140 150
MASLLKKELE QMEDFFLDAP LLPPPSPPPP PPPAAAPSLP LPLPLPTFDL
160 170 180 190 200
PQPPTLDTLD LLAVYCRSEA GPGDSGLSTL PVPQQPPPLA PLPSPARPAP
210 220 230 240 250
YPSPASTRGD RKQKKRDQNK SAALRYRQRK RAEGEALEGE CQGLEARNRE
260 270 280
LRERAESVER EIQYVKDLLI EVYKARSQRT RST
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A140LIB7 | A0A140LIB7_MOUSE | Cyclic AMP-dependent transcription ... | Atf5 | 75 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 146 | P → PLP in AAK00584 (PubMed:11278933).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF375475 mRNA Translation: AAK54640.1 AF375476 mRNA Translation: AAK54641.1 AF312938 mRNA Translation: AAG34160.1 AF310624 mRNA Translation: AAL55820.1 AY115107 Genomic DNA Translation: AAM74470.1 BC010195 mRNA Translation: AAH10195.1 BC092068 mRNA Translation: AAH92068.1 AY024362 mRNA Translation: AAK00584.1 AB012276 mRNA Translation: BAA25313.1 |
CCDSi | CCDS21215.1 |
RefSeqi | NP_109618.1, NM_030693.2 |
Genome annotation databases
Ensembli | ENSMUST00000047356; ENSMUSP00000047771; ENSMUSG00000038539 ENSMUST00000107893; ENSMUSP00000103525; ENSMUSG00000038539 |
GeneIDi | 107503 |
KEGGi | mmu:107503 |
UCSCi | uc009gqu.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF375475 mRNA Translation: AAK54640.1 AF375476 mRNA Translation: AAK54641.1 AF312938 mRNA Translation: AAG34160.1 AF310624 mRNA Translation: AAL55820.1 AY115107 Genomic DNA Translation: AAM74470.1 BC010195 mRNA Translation: AAH10195.1 BC092068 mRNA Translation: AAH92068.1 AY024362 mRNA Translation: AAK00584.1 AB012276 mRNA Translation: BAA25313.1 |
CCDSi | CCDS21215.1 |
RefSeqi | NP_109618.1, NM_030693.2 |
3D structure databases
AlphaFoldDBi | O70191 |
SMRi | O70191 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 223346, 2 interactors |
IntActi | O70191, 1 interactor |
STRINGi | 10090.ENSMUSP00000047771 |
PTM databases
iPTMneti | O70191 |
PhosphoSitePlusi | O70191 |
Proteomic databases
PaxDbi | O70191 |
PRIDEi | O70191 |
Protocols and materials databases
Antibodypediai | 18799, 296 antibodies from 33 providers |
DNASUi | 107503 |
Genome annotation databases
Ensembli | ENSMUST00000047356; ENSMUSP00000047771; ENSMUSG00000038539 ENSMUST00000107893; ENSMUSP00000103525; ENSMUSG00000038539 |
GeneIDi | 107503 |
KEGGi | mmu:107503 |
UCSCi | uc009gqu.2, mouse |
Organism-specific databases
CTDi | 22809 |
MGIi | MGI:2141857, Atf5 |
VEuPathDBi | HostDB:ENSMUSG00000038539 |
Phylogenomic databases
eggNOGi | KOG4571, Eukaryota |
GeneTreei | ENSGT00530000063801 |
HOGENOMi | CLU_083640_0_0_1 |
InParanoidi | O70191 |
OMAi | KIPGHYE |
OrthoDBi | 1524842at2759 |
PhylomeDBi | O70191 |
TreeFami | TF316136 |
Miscellaneous databases
BioGRID-ORCSi | 107503, 1 hit in 74 CRISPR screens |
PROi | PR:O70191 |
RNActi | O70191, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038539, Expressed in olfactory epithelium and 316 other tissues |
Genevisiblei | O70191, MM |
Family and domain databases
InterProi | View protein in InterPro IPR029855, ATF5 IPR004827, bZIP IPR046347, bZIP_sf |
PANTHERi | PTHR13044:SF3, PTHR13044:SF3, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATF5_MOUSE | |
Accessioni | O70191Primary (citable) accession number: O70191 Secondary accession number(s): Q58E51, Q99NH6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | February 21, 2001 | |
Last modified: | May 25, 2022 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families