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Protein

Neuronal acetylcholine receptor subunit alpha-4

Gene

Chrna4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.

GO - Molecular functioni

  • acetylcholine binding Source: MGI
  • acetylcholine-gated cation-selective channel activity Source: MGI
  • acetylcholine receptor activity Source: MGI
  • drug binding Source: MGI
  • ion channel activity Source: MGI
  • protein heterodimerization activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-629587 Highly sodium permeable acetylcholine nicotinic receptors
R-MMU-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-MMU-629597 Highly calcium permeable nicotinic acetylcholine receptors

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-4
Gene namesi
Name:Chrna4
Synonyms:Acra4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:87888 Chrna4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 249ExtracellularSequence analysisAdd BLAST218
Transmembranei250 – 270HelicalSequence analysisAdd BLAST21
Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
Transmembranei312 – 332HelicalSequence analysisAdd BLAST21
Topological domaini333 – 603CytoplasmicSequence analysisAdd BLAST271
Transmembranei604 – 624HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4457

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000000035231 – 629Neuronal acetylcholine receptor subunit alpha-4Add BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi163 ↔ 177By similarity
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi227 ↔ 228Associated with receptor activationBy similarity
Lipidationi273S-palmitoyl cysteineBy similarity1
Modified residuei427PhosphoserineBy similarity1
Modified residuei540PhosphoserineCombined sources1
Modified residuei543PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiO70174
PRIDEiO70174

PTM databases

iPTMnetiO70174
PhosphoSitePlusiO70174

Expressioni

Gene expression databases

BgeeiENSMUSG00000027577 Expressed in 117 organ(s), highest expression level in retina
ExpressionAtlasiO70174 baseline and differential
GenevisibleiO70174 MM

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors, complexes with beta-2 may be heteropentamers. Interacts with RIC3; which is required for proper folding and assembly. Interacts with LYPD6. The heteropentamer alpha-4-beta-2 interacts with alpha-conotoxins PnIA, GID and MII (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Lypd1Q8BLC33EBI-10916203,EBI-14035010

GO - Molecular functioni

Protein-protein interaction databases

BioGridi197932, 2 interactors
ComplexPortaliCPX-167 Neuronal nicotinic acetylcholine receptor complex, 3xalpha4-2xbeta2
CPX-169 Neuronal nicotinic acetylcholine receptor complex, 2xalpha4-3xbeta2
CPX-216 Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2
CPX-220 Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4
DIPiDIP-48731N
IntActiO70174, 6 interactors
STRINGi10090.ENSMUSP00000066338

Structurei

3D structure databases

ProteinModelPortaliO70174
SMRiO70174
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiO70174
KOiK04806
OMAiYECCTEI
PhylomeDBiO70174
TreeFamiTF315605

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O70174-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEIGGSGAPP PLLLLPLLLL LGTGLLPASS HIETRAHAEE RLLKRLFSGY
60 70 80 90 100
NKWSRPVANI SDVVLVRFGL SIAQLIDVDE KNQMMTTNVW VKQEWHDYKL
110 120 130 140 150
RWDPGDYENV TSIRIPSELI WRPDIVLYNN ADGDFAVTHL TKAHLFYDGR
160 170 180 190 200
VQWTPPAIYK SSCSIDVTFF PFDQQNCTMK FGSWTYDKAK IDLVSMHSRV
210 220 230 240 250
DQLDFWESGE WVIVDAVGTY NTRKYECCAE IYPDITYAFI IRRLPLFYTI
260 270 280 290 300
NLIIPCLLIS CLTVLVFYLP SECGEKVTLC ISVLLSLTVF LLLITEIIPS
310 320 330 340 350
TSLVIPLIGE YLLFTMIFVT LSIVITVFVL NVHHRSPRTH TMPAWVRRVF
360 370 380 390 400
LDIVPRLLFM KRPSVVKDNC RRLIESMHKM ANAPRFWPEP ESEPGILGDI
410 420 430 440 450
CNQGLSPAPT FCNRMDTAVE TQPTCRSPSH KVPDLKTSEV EKASPCPSPG
460 470 480 490 500
SCHPPNSSGA PVLIKARSLS VQHVPSSQEA AEGSIRCRSR SIQYCVSQDG
510 520 530 540 550
AASLTESKPT GSPASLKTRP SQLPVSDQTS PCKCTCKEPS PVSPITVLKA
560 570 580 590 600
GGTKAPPQHL PLSPALTRAV EGVQYIADHL KAEDTDFSVK EDWKYVAMVI
610 620
DRIFLWMFII VCLLGTVGLF LPPWLAGMI
Length:629
Mass (Da):70,305
Last modified:September 19, 2003 - v2
Checksum:i261455B6ED50B41C
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7ZBU7B7ZBU7_MOUSE
Neuronal acetylcholine receptor sub...
Chrna4
641Annotation score:
A0A0G2JFP3A0A0G2JFP3_MOUSE
Neuronal acetylcholine receptor sub...
Chrna4
125Annotation score:
B7ZBV1B7ZBV1_MOUSE
Neuronal acetylcholine receptor sub...
Chrna4
78Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16P → L in AAF34716 (PubMed:11434511).Curated1
Sequence conflicti24G → A in BAA25752 (PubMed:9749753).Curated1
Sequence conflicti134D → N in AAF34716 (PubMed:11434511).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti529T → A1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225912 mRNA Translation: AAF34716.2
AK034228 mRNA Translation: BAC28638.1
AK083157 mRNA Translation: BAC38788.1
BC053013 mRNA Translation: AAH53013.1
AB010002 Genomic DNA Translation: BAA25752.1
AF325347 mRNA Translation: AAL37363.1
CCDSiCCDS17192.1
RefSeqiNP_056545.3, NM_015730.5
UniGeneiMm.252369

Genome annotation databases

EnsembliENSMUST00000067120; ENSMUSP00000066338; ENSMUSG00000027577
GeneIDi11438
KEGGimmu:11438
UCSCiuc008okq.1 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225912 mRNA Translation: AAF34716.2
AK034228 mRNA Translation: BAC28638.1
AK083157 mRNA Translation: BAC38788.1
BC053013 mRNA Translation: AAH53013.1
AB010002 Genomic DNA Translation: BAA25752.1
AF325347 mRNA Translation: AAL37363.1
CCDSiCCDS17192.1
RefSeqiNP_056545.3, NM_015730.5
UniGeneiMm.252369

3D structure databases

ProteinModelPortaliO70174
SMRiO70174
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197932, 2 interactors
ComplexPortaliCPX-167 Neuronal nicotinic acetylcholine receptor complex, 3xalpha4-2xbeta2
CPX-169 Neuronal nicotinic acetylcholine receptor complex, 2xalpha4-3xbeta2
CPX-216 Neuronal nicotinic acetylcholine receptor complex, alpha4-alpha5-beta2
CPX-220 Neuronal nicotinic acetylcholine receptor complex, alpha4-beta4
DIPiDIP-48731N
IntActiO70174, 6 interactors
STRINGi10090.ENSMUSP00000066338

Chemistry databases

ChEMBLiCHEMBL4457

PTM databases

iPTMnetiO70174
PhosphoSitePlusiO70174

Proteomic databases

PaxDbiO70174
PRIDEiO70174

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067120; ENSMUSP00000066338; ENSMUSG00000027577
GeneIDi11438
KEGGimmu:11438
UCSCiuc008okq.1 mouse

Organism-specific databases

CTDi1137
MGIiMGI:87888 Chrna4

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
GeneTreeiENSGT00760000118930
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiO70174
KOiK04806
OMAiYECCTEI
PhylomeDBiO70174
TreeFamiTF315605

Enzyme and pathway databases

ReactomeiR-MMU-629587 Highly sodium permeable acetylcholine nicotinic receptors
R-MMU-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-MMU-629597 Highly calcium permeable nicotinic acetylcholine receptors

Miscellaneous databases

ChiTaRSiChrna4 mouse
PROiPR:O70174
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027577 Expressed in 117 organ(s), highest expression level in retina
ExpressionAtlasiO70174 baseline and differential
GenevisibleiO70174 MM

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 1 hit
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACHA4_MOUSE
AccessioniPrimary (citable) accession number: O70174
Secondary accession number(s): Q8BHE9, Q8VI10, Q9ET51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 19, 2003
Last modified: November 7, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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