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Protein

DNA topoisomerase 3-alpha

Gene

Top3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei362O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri658 – 685C4-typeSequence analysisAdd BLAST28

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA topoisomerase activity Source: GO_Central
  • DNA topoisomerase type I activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3-alpha (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III alpha
Gene namesi
Name:Top3a
Synonyms:Top3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1197527 Top3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451911 – 1003DNA topoisomerase 3-alphaAdd BLAST1003

Proteomic databases

EPDiO70157
PaxDbiO70157
PeptideAtlasiO70157
PRIDEiO70157

PTM databases

iPTMnetiO70157
PhosphoSitePlusiO70157

Expressioni

Tissue specificityi

Highly expressed in testis.

Gene expression databases

BgeeiENSMUSG00000002814 Expressed in 216 organ(s), highest expression level in cardiac ventricle
CleanExiMM_TOP3A
ExpressionAtlasiO70157 baseline and differential
GenevisibleiO70157 MM

Interactioni

Subunit structurei

Interacts (via N-terminal region) with BLM; the interaction is direct. Directly interacts with RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM (By similarity).By similarity

Protein-protein interaction databases

BioGridi204278, 8 interactors
ComplexPortaliCPX-3303 BTR double Holliday Junction dissolution complex
IntActiO70157, 9 interactors
STRINGi10090.ENSMUSP00000002891

Structurei

3D structure databases

ProteinModelPortaliO70157
SMRiO70157
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 179ToprimPROSITE-ProRule annotationAdd BLAST145
Repeati814 – 8411Add BLAST28
Repeati898 – 9252Add BLAST28

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni814 – 9252 X 27 AA approximate repeatsAdd BLAST112

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri658 – 685C4-typeSequence analysisAdd BLAST28

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1956 Eukaryota
COG0550 LUCA
GeneTreeiENSGT00530000063430
HOGENOMiHOG000184377
HOVERGENiHBG056668
InParanoidiO70157
KOiK03165
OrthoDBiEOG091G03BD
PhylomeDBiO70157
TreeFamiTF105287

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 1 hit
2.70.20.10, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR013498 Topo_IA_Znf
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
IPR010666 Znf_GRF
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF01396 zf-C4_Topoisom, 1 hit
PF06839 zf-GRF, 2 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O70157-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIFPVTLLAF QWHRRPGGRA LSRAAMEVAF RGVRKVLCVA EKNDAAKGIA
60 70 80 90 100
DLLSNGRMRR KEGLSKFNKI YEFDYHLYGQ NVTMIMTSVS GHLLAHDFQM
110 120 130 140 150
QFRKWQSCNP LVLFEAEIEK YCPENFIDIK KTLERETHHC QALVIWTDCD
160 170 180 190 200
REGENIGFEI IHVCKAVKPN LRVLRARFSE ITPHAVRTAC ENLTEPDQRV
210 220 230 240 250
SDAVDVRQEL DLRIGAAFTR FQTLRLQRIF PEVLAEQLIS YGSCQFPTLG
260 270 280 290 300
FVVERFKAIQ AFVPEVFHKI KVTHDHKDGT VEFNWKRYRL FNHTACLVLY
310 320 330 340 350
QLCMEDPMAT VVEVRSKPKS KWRPQALDTV ELEKLASRKL RINAKETMRI
360 370 380 390 400
AEKLYTQGYI SYPRTETNIF PKDLNLVALV EQQTVDPHWG AFAQTILERG
410 420 430 440 450
GPTPRNGSKS DQAHPPIHPT KYTSGLQGDD RRLYEFIVRH FLACCSQDAQ
460 470 480 490 500
GQETTVEIDI AQERFVAHGL IILARNYLDV YPYDHWSDKL LPVYEQGSHF
510 520 530 540 550
QPSTVEMVDG ETSPPQLLTE ADLIALMEKH GIGTDATHAE HIETIKARMY
560 570 580 590 600
VGLTSDKRFL PGHLGMGLVE GYDSMGYEMS KPDLRAELEA DLKLICEGKK
610 620 630 640 650
DKFQVLRQQV QKYKQVFIEA VAKAKKLDEA LSQYLGERTE MAQQEEIYPA
660 670 680 690 700
MPEPVRKCPQ CNKDMVLKTK KSGGFYLSCM GFPECRSAVW FPDSVLEASR
710 720 730 740 750
DNSVCSVCQP PPVYRLKLKF KRGSLPPAMP LEFVGCIGGC DETLKEIFGL
760 770 780 790 800
RFPRALPRAS QPSGHLQASQ ALNRMDSSQH NLSQPLVNRH TRPSKTVAQA
810 820 830 840 850
LLPPTTAGES NSVTCNCGRE AVLLTVRKQG PNQGRHFYKC SNGDCNFFLW
860 870 880 890 900
ADSSHSTGGG TPTSASGPPG SSVGCPSSVG SHMDGFGSLG SDSDGGTPCL
910 920 930 940 950
CGQPAVTRTV QKDGPNKGRQ FHTCAKPREQ QCGFFQWVDE NVAPGSFAAP
960 970 980 990 1000
AWPGGRGKAQ RPEAASKRPR AGSSDAGSTV KKPRKCSLCH QPGHTRTFCP

QNR
Length:1,003
Mass (Da):112,358
Last modified:August 1, 1998 - v1
Checksum:i254C738E746EE495
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3V3P1Q3V3P1_MOUSE
DNA topoisomerase
Top3a
954Annotation score:
B0QZI1B0QZI1_MOUSE
DNA topoisomerase
Top3a
670Annotation score:
Q5NCT0Q5NCT0_MOUSE
DNA topoisomerase
Top3a
773Annotation score:
Q5NCT1Q5NCT1_MOUSE
DNA topoisomerase
Top3a
85Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006074 mRNA Translation: BAA25662.1
CCDSiCCDS24797.1
PIRiT13951
RefSeqiNP_033436.1, NM_009410.2
UniGeneiMm.10708
Mm.477819

Genome annotation databases

EnsembliENSMUST00000002891; ENSMUSP00000002891; ENSMUSG00000002814
GeneIDi21975
KEGGimmu:21975
UCSCiuc007jgl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006074 mRNA Translation: BAA25662.1
CCDSiCCDS24797.1
PIRiT13951
RefSeqiNP_033436.1, NM_009410.2
UniGeneiMm.10708
Mm.477819

3D structure databases

ProteinModelPortaliO70157
SMRiO70157
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204278, 8 interactors
ComplexPortaliCPX-3303 BTR double Holliday Junction dissolution complex
IntActiO70157, 9 interactors
STRINGi10090.ENSMUSP00000002891

PTM databases

iPTMnetiO70157
PhosphoSitePlusiO70157

Proteomic databases

EPDiO70157
PaxDbiO70157
PeptideAtlasiO70157
PRIDEiO70157

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002891; ENSMUSP00000002891; ENSMUSG00000002814
GeneIDi21975
KEGGimmu:21975
UCSCiuc007jgl.1 mouse

Organism-specific databases

CTDi7156
MGIiMGI:1197527 Top3a

Phylogenomic databases

eggNOGiKOG1956 Eukaryota
COG0550 LUCA
GeneTreeiENSGT00530000063430
HOGENOMiHOG000184377
HOVERGENiHBG056668
InParanoidiO70157
KOiK03165
OrthoDBiEOG091G03BD
PhylomeDBiO70157
TreeFamiTF105287

Enzyme and pathway databases

ReactomeiR-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Miscellaneous databases

PROiPR:O70157
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002814 Expressed in 216 organ(s), highest expression level in cardiac ventricle
CleanExiMM_TOP3A
ExpressionAtlasiO70157 baseline and differential
GenevisibleiO70157 MM

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 1 hit
2.70.20.10, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR013498 Topo_IA_Znf
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
IPR010666 Znf_GRF
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF01396 zf-C4_Topoisom, 1 hit
PF06839 zf-GRF, 2 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTOP3A_MOUSE
AccessioniPrimary (citable) accession number: O70157
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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