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Protein

Dual specificity testis-specific protein kinase 1

Gene

Tesk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Probably plays a central role at and after the meiotic phase of spermatogenesis (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Protein has several cofactor binding sites:

Activity regulationi

Activated by autophosphorylation on Ser-215. Kinase activity is inhibited by SPRED1 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81ATPPROSITE-ProRule annotation1
Active sitei170Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 66ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity testis-specific protein kinase 1 (EC:2.7.12.1)
Alternative name(s):
Testicular protein kinase 1
Gene namesi
Name:Tesk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1201675 Tesk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867471 – 627Dual specificity testis-specific protein kinase 1Add BLAST627

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei215Phosphoserine; by autocatalysisBy similarity1
Modified residuei338Omega-N-methylarginineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiO70146
PaxDbiO70146
PRIDEiO70146

PTM databases

iPTMnetiO70146
PhosphoSitePlusiO70146
SwissPalmiO70146

Expressioni

Tissue specificityi

Predominantly expressed in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000028458 Expressed in 252 organ(s), highest expression level in testis
CleanExiMM_TESK1
ExpressionAtlasiO70146 baseline and differential
GenevisibleiO70146 MM

Interactioni

Subunit structurei

Interacts with SPRY4. Interacts with TAOK2; leading to inhibit TAOK2 activity. Interacts with SPRED1; leading to inhibit activity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204120, 1 interactor
STRINGi10090.ENSMUSP00000050087

Structurei

3D structure databases

ProteinModelPortaliO70146
SMRiO70146
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 309Protein kinasePROSITE-ProRule annotationAdd BLAST258

Domaini

The extracatalytic C-terminal part is highly rich in proline residues.

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IF1S Eukaryota
COG0515 LUCA
GeneTreeiENSGT00530000063025
HOGENOMiHOG000231415
HOVERGENiHBG058204
InParanoidiO70146
KOiK08841
OMAiCHSLPCT
OrthoDBiEOG091G0HLL
PhylomeDBiO70146
TreeFamiTF318014

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR015782 TESK1
IPR008266 Tyr_kinase_AS
PANTHERiPTHR44164:SF3 PTHR44164:SF3, 1 hit
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00109 TYRKINASE
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O70146-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGERPPLRG PGPGEAPGEG PGGAGGGPGR GRPSSYRALR SAVSSLARVD
60 70 80 90 100
DFDCAEKIGA GFFSEVYKVR HRQSGQVMVL KMNKLPSNRS NTLREVQLMN
110 120 130 140 150
RLRHPNILRF MGVCVHQGQL HALTEYMNGG TLEQLLSSPE PLSWPVRLHL
160 170 180 190 200
ALDIAQGLRY LHAKGVFHRD LTSKNCLVRR EDRGFTAVVG DFGLAEKIPV
210 220 230 240 250
YREGTRKEPL AVVGSPYWMA PEVLRGELYD EKADVFAFGI VLCELIARVP
260 270 280 290 300
ADPDYLPRTE DFGLDVPAFR TLVGNDCPLP FLLLAIHCCS MEPSTRAPFT
310 320 330 340 350
EITQHLEQIL EQQPEATPLA KPPLTKAPLT YNQGSVPRGG PSATLPRPDP
360 370 380 390 400
RLSRSRSDLF LPPSPESPPS WGDNLTRVNP FSLREDLRGG KIKLLDTPCK
410 420 430 440 450
PATPLPLVPP SPLTSTQLPL VTTPDILVQP ETPVRRCRSL PSSPELPRRM
460 470 480 490 500
ETALPGPGPS PMGPTEERMD CEGSSPEPEP PGLAPQLPLA VATDNFISTC
510 520 530 540 550
SSASQPWSPR SGPPLNNNPP AVVVNSPQGW AREPWNRAQH SLPRAAALER
560 570 580 590 600
TEPSPPPSAP REPEEGLPCP GCCLGPFSFG FLSMCPRPTP AVARYRNLNC
610 620
EAGSLLCHRG HHAKPPTPSL QLPGARS
Length:627
Mass (Da):68,052
Last modified:October 23, 2007 - v3
Checksum:i091ADA8CF52A2B50
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RFP6D6RFP6_MOUSE
Dual-specificity testis-specific pr...
Tesk1
201Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti550R → Q in BAA25124 (PubMed:9469938).Curated1
Sequence conflicti550R → Q in BAA25125 (PubMed:9469938).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003493 Genomic DNA Translation: BAA25124.1
AB003494 mRNA Translation: BAA25125.1
AK144302 mRNA Translation: BAE25822.1
AL732506 Genomic DNA Translation: CAM17173.1
BC099699 mRNA Translation: AAH99699.1
CCDSiCCDS18094.1
PIRiJC6534
RefSeqiNP_035701.3, NM_011571.3
UniGeneiMm.485441

Genome annotation databases

EnsembliENSMUST00000060864; ENSMUSP00000050087; ENSMUSG00000028458
GeneIDi21754
KEGGimmu:21754
UCSCiuc008spq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003493 Genomic DNA Translation: BAA25124.1
AB003494 mRNA Translation: BAA25125.1
AK144302 mRNA Translation: BAE25822.1
AL732506 Genomic DNA Translation: CAM17173.1
BC099699 mRNA Translation: AAH99699.1
CCDSiCCDS18094.1
PIRiJC6534
RefSeqiNP_035701.3, NM_011571.3
UniGeneiMm.485441

3D structure databases

ProteinModelPortaliO70146
SMRiO70146
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204120, 1 interactor
STRINGi10090.ENSMUSP00000050087

PTM databases

iPTMnetiO70146
PhosphoSitePlusiO70146
SwissPalmiO70146

Proteomic databases

MaxQBiO70146
PaxDbiO70146
PRIDEiO70146

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060864; ENSMUSP00000050087; ENSMUSG00000028458
GeneIDi21754
KEGGimmu:21754
UCSCiuc008spq.2 mouse

Organism-specific databases

CTDi7016
MGIiMGI:1201675 Tesk1

Phylogenomic databases

eggNOGiENOG410IF1S Eukaryota
COG0515 LUCA
GeneTreeiENSGT00530000063025
HOGENOMiHOG000231415
HOVERGENiHBG058204
InParanoidiO70146
KOiK08841
OMAiCHSLPCT
OrthoDBiEOG091G0HLL
PhylomeDBiO70146
TreeFamiTF318014

Enzyme and pathway databases

BRENDAi2.7.10.2 3474

Miscellaneous databases

PROiPR:O70146
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028458 Expressed in 252 organ(s), highest expression level in testis
CleanExiMM_TESK1
ExpressionAtlasiO70146 baseline and differential
GenevisibleiO70146 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR015782 TESK1
IPR008266 Tyr_kinase_AS
PANTHERiPTHR44164:SF3 PTHR44164:SF3, 1 hit
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00109 TYRKINASE
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTESK1_MOUSE
AccessioniPrimary (citable) accession number: O70146
Secondary accession number(s): O70147, Q499W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 23, 2007
Last modified: November 7, 2018
This is version 158 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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