UniProtKB - O70145 (NCF2_MOUSE)
Protein
Neutrophil cytosol factor 2
Gene
Ncf2
Organism
Mus musculus (Mouse)
Status
Functioni
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).By similarity
GO - Molecular functioni
- protein C-terminus binding Source: MGI
- Rac GTPase binding Source: MGI
- superoxide-generating NAD(P)H oxidase activity Source: MGI
- superoxide-generating NADPH oxidase activator activity Source: GO_Central
GO - Biological processi
- cellular response to hormone stimulus Source: MGI
- NADP catabolic process Source: MGI
- oxidation-reduction process Source: MGI
- phagocytosis Source: InterPro
- positive regulation of blood pressure Source: MGI
- positive regulation of neuron apoptotic process Source: MGI
- respiratory burst Source: InterPro
- response to glucose Source: MGI
- response to lipopolysaccharide Source: MGI
- response to organic cyclic compound Source: MGI
- superoxide anion generation Source: MGI
- superoxide metabolic process Source: MGI
Enzyme and pathway databases
Reactomei | R-MMU-1222556, ROS and RNS production in phagocytes R-MMU-1236973, Cross-presentation of particulate exogenous antigens (phagosomes) R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-4420097, VEGFA-VEGFR2 Pathway R-MMU-5668599, RHO GTPases Activate NADPH Oxidases |
Names & Taxonomyi
Protein namesi | Recommended name: Neutrophil cytosol factor 2Short name: NCF-2 Alternative name(s): 67 kDa neutrophil oxidase factor NADPH oxidase activator 2 Neutrophil NADPH oxidase factor 2 p67-phox |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97284, Ncf2 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleolus Source: MGI
Plasma Membrane
- NADPH oxidase complex Source: MGI
Other locations
- acrosomal vesicle Source: MGI
- cytoplasm Source: MGI
- membrane Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000312227 | 1 – 525 | Neutrophil cytosol factor 2Add BLAST | 525 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 233 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 324 | PhosphoserineCombined sources | 1 | |
Modified residuei | 398 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O70145 |
jPOSTi | O70145 |
MaxQBi | O70145 |
PaxDbi | O70145 |
PeptideAtlasi | O70145 |
PRIDEi | O70145 |
PTM databases
iPTMneti | O70145 |
PhosphoSitePlusi | O70145 |
SwissPalmi | O70145 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026480, Expressed in granulocyte and 57 other tissues |
Genevisiblei | O70145, MM |
Interactioni
Subunit structurei
Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4.
Interacts with NCF4.
Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus).
Interacts with SYTL1 and RAC1. May interact with NOXO1.
Interacts with S100A8 and calprotectin (S100A8/9) (By similarity).
By similarityBinary interactionsi
O70145
With | #Exp. | IntAct |
---|---|---|
Prdx6 [O08709] | 3 | EBI-9550667,EBI-444895 |
GO - Molecular functioni
- protein C-terminus binding Source: MGI
- Rac GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201702, 3 interactors |
IntActi | O70145, 2 interactors |
STRINGi | 10090.ENSMUSP00000140404 |
Miscellaneous databases
RNActi | O70145, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 37 – 70 | TPR 1Sequence analysisAdd BLAST | 34 | |
Repeati | 71 – 104 | TPR 2Sequence analysisAdd BLAST | 34 | |
Repeati | 121 – 154 | TPR 3Sequence analysisAdd BLAST | 34 | |
Domaini | 240 – 299 | SH3 1PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 350 – 428 | PB1PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 456 – 515 | SH3 2PROSITE-ProRule annotationAdd BLAST | 60 |
Domaini
The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity
Sequence similaritiesi
Belongs to the NCF2/NOXA1 family.Curated
Keywords - Domaini
Repeat, SH3 domain, TPR repeatPhylogenomic databases
eggNOGi | KOG4225, Eukaryota |
GeneTreei | ENSGT00530000063843 |
HOGENOMi | CLU_041290_0_0_1 |
InParanoidi | O70145 |
OMAi | YYQMEKY |
OrthoDBi | 431898at2759 |
PhylomeDBi | O70145 |
TreeFami | TF329087 |
Family and domain databases
CDDi | cd06406, PB1_P67, 1 hit cd11871, SH3_p67phox_N, 1 hit |
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR034889, P67-PHOX IPR035546, p67phox_SH3_1 IPR000270, PB1_dom IPR034885, PB1_P67 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR001440, TPR_1 IPR019734, TPR_repeat |
PANTHERi | PTHR15175:SF3, PTHR15175:SF3, 1 hit |
Pfami | View protein in Pfam PF00564, PB1, 1 hit PF00018, SH3_1, 2 hits PF00515, TPR_1, 1 hit PF13181, TPR_8, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00666, PB1, 1 hit SM00326, SH3, 2 hits SM00028, TPR, 3 hits |
SUPFAMi | SSF48452, SSF48452, 1 hit SSF50044, SSF50044, 2 hits |
PROSITEi | View protein in PROSITE PS51745, PB1, 1 hit PS50002, SH3, 2 hits PS50005, TPR, 3 hits PS50293, TPR_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O70145-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLAEAIRLW NEGVLAADKK DWKGALEAFS EVQDPHSRIC FNIGCVNTIL
60 70 80 90 100
ENLQAAEQAF TKSINRDKHS AVAYFQRGML YYRMEKYDLA IKDLKEALTQ
110 120 130 140 150
LRGNQLIDYK ILGLQFKLFA CEVLYNIALM HAKKEEWKKA EEQLALATNM
160 170 180 190 200
KSEPRHSKID KAMESIWKQK LFEPVVIPVG RLFRPNERQV AQLAKKDYLG
210 220 230 240 250
KATVVASVVH QDNFSGFAPL QPQSAEPPPR PKTPEIFRAL EGEAHRVLFG
260 270 280 290 300
FVPETPEELQ VMPGNIVFVL KKGSDNWATV MFNGQKGLVP CNYLEPVELR
310 320 330 340 350
IHPQSQPQED TSPESDIPPP PNSSPPGRLQ LSPGHKQKEP KELKLSVPMP
360 370 380 390 400
YMLKVHYKYT VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE
410 420 430 440 450
LLLLSEESMK DAWGQVKNYC LTLWCEHTVG DQGLIDEPIQ RENSDASKQT
460 470 480 490 500
TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG DVILVLSHVN EEWLEGECKG
510 520
KVGIFPKAFV EGCAAKNLEG IPREV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WPH0 | A0A087WPH0_MOUSE | Neutrophil cytosol factor 2 | Ncf2 | 114 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | E → A in BAE42353 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 31 | E → A in BAE42218 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 31 | E → A in BAE42065 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 31 | E → A in BAE33191 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 324 | S → N in BAE32002 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB002664 mRNA Translation: BAA25650.1 AK036379 mRNA Translation: BAC29404.1 AK137152 mRNA Translation: BAE23254.1 AK153447 mRNA Translation: BAE32002.1 AK155324 mRNA Translation: BAE33191.1 AK170839 mRNA Translation: BAE42065.1 AK171059 mRNA Translation: BAE42218.1 AK171265 mRNA Translation: BAE42353.1 BC003730 mRNA Translation: AAH03730.1 |
CCDSi | CCDS15367.1 |
RefSeqi | NP_035007.1, NM_010877.5 XP_006529299.1, XM_006529236.3 |
Genome annotation databases
Ensembli | ENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480 ENSMUST00000186568; ENSMUSP00000140404; ENSMUSG00000026480 |
GeneIDi | 17970 |
KEGGi | mmu:17970 |
UCSCi | uc007czm.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB002664 mRNA Translation: BAA25650.1 AK036379 mRNA Translation: BAC29404.1 AK137152 mRNA Translation: BAE23254.1 AK153447 mRNA Translation: BAE32002.1 AK155324 mRNA Translation: BAE33191.1 AK170839 mRNA Translation: BAE42065.1 AK171059 mRNA Translation: BAE42218.1 AK171265 mRNA Translation: BAE42353.1 BC003730 mRNA Translation: AAH03730.1 |
CCDSi | CCDS15367.1 |
RefSeqi | NP_035007.1, NM_010877.5 XP_006529299.1, XM_006529236.3 |
3D structure databases
SMRi | O70145 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201702, 3 interactors |
IntActi | O70145, 2 interactors |
STRINGi | 10090.ENSMUSP00000140404 |
PTM databases
iPTMneti | O70145 |
PhosphoSitePlusi | O70145 |
SwissPalmi | O70145 |
Proteomic databases
EPDi | O70145 |
jPOSTi | O70145 |
MaxQBi | O70145 |
PaxDbi | O70145 |
PeptideAtlasi | O70145 |
PRIDEi | O70145 |
Protocols and materials databases
Antibodypediai | 702, 266 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480 ENSMUST00000186568; ENSMUSP00000140404; ENSMUSG00000026480 |
GeneIDi | 17970 |
KEGGi | mmu:17970 |
UCSCi | uc007czm.1, mouse |
Organism-specific databases
CTDi | 4688 |
MGIi | MGI:97284, Ncf2 |
Phylogenomic databases
eggNOGi | KOG4225, Eukaryota |
GeneTreei | ENSGT00530000063843 |
HOGENOMi | CLU_041290_0_0_1 |
InParanoidi | O70145 |
OMAi | YYQMEKY |
OrthoDBi | 431898at2759 |
PhylomeDBi | O70145 |
TreeFami | TF329087 |
Enzyme and pathway databases
Reactomei | R-MMU-1222556, ROS and RNS production in phagocytes R-MMU-1236973, Cross-presentation of particulate exogenous antigens (phagosomes) R-MMU-3299685, Detoxification of Reactive Oxygen Species R-MMU-4420097, VEGFA-VEGFR2 Pathway R-MMU-5668599, RHO GTPases Activate NADPH Oxidases |
Miscellaneous databases
BioGRID-ORCSi | 17970, 0 hits in 17 CRISPR screens |
ChiTaRSi | Ncf2, mouse |
PROi | PR:O70145 |
RNActi | O70145, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026480, Expressed in granulocyte and 57 other tissues |
Genevisiblei | O70145, MM |
Family and domain databases
CDDi | cd06406, PB1_P67, 1 hit cd11871, SH3_p67phox_N, 1 hit |
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR034889, P67-PHOX IPR035546, p67phox_SH3_1 IPR000270, PB1_dom IPR034885, PB1_P67 IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR001440, TPR_1 IPR019734, TPR_repeat |
PANTHERi | PTHR15175:SF3, PTHR15175:SF3, 1 hit |
Pfami | View protein in Pfam PF00564, PB1, 1 hit PF00018, SH3_1, 2 hits PF00515, TPR_1, 1 hit PF13181, TPR_8, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00666, PB1, 1 hit SM00326, SH3, 2 hits SM00028, TPR, 3 hits |
SUPFAMi | SSF48452, SSF48452, 1 hit SSF50044, SSF50044, 2 hits |
PROSITEi | View protein in PROSITE PS51745, PB1, 1 hit PS50002, SH3, 2 hits PS50005, TPR, 3 hits PS50293, TPR_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCF2_MOUSE | |
Accessioni | O70145Primary (citable) accession number: O70145 Secondary accession number(s): Q3TC92, Q3U5S4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 4, 2007 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families