Ncf2

Functionⁱ

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).By similarity

GO - Molecular functionⁱ

protein C-terminus binding Source: MGI
Rac GTPase binding
Source: MGI
Inferred from physical interactionⁱ
- 9490028
superoxide-generating NADPH oxidase activator activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular response to hormone stimulus Source: MGI
NADP catabolic process
Source: MGI
Inferred from sequence orthologyⁱ
- 10647999
oxidation-reduction process Source: GOC
phagocytosis Source: InterPro
positive regulation of blood pressure Source: MGI
positive regulation of neuron apoptotic process Source: MGI
respiratory burst Source: InterPro
response to glucose Source: MGI
response to lipopolysaccharide Source: MGI
response to organic cyclic compound Source: MGI
superoxide anion generation
Source: MGI
Inferred from direct assayⁱ
- 9490028
superoxide metabolic process
Source: MGI
Inferred from sequence orthologyⁱ
- 10647999

View the complete GO annotation on QuickGO ...

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1222556 ROS, RNS production in phagocytes R-MMU-1236973 Cross-presentation of particulate exogenous antigens (phagosomes) R-MMU-3299685 Detoxification of Reactive Oxygen Species R-MMU-4420097 VEGFA-VEGFR2 Pathway R-MMU-5668599 RHO GTPases Activate NADPH Oxidases

Reactomeⁱ

R-MMU-1222556 ROS, RNS production in phagocytes
R-MMU-1236973 Cross-presentation of particulate exogenous antigens (phagosomes)
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5668599 RHO GTPases Activate NADPH Oxidases

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Neutrophil cytosol factor 2 Short name: NCF-2 Alternative name(s): 67 kDa neutrophil oxidase factor NADPH oxidase activator 2 Neutrophil NADPH oxidase factor 2 p67-phox
Gene namesⁱ	Name:Ncf2Imported Synonyms:Noxa2, P67phox
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 1

Organism-specific databases

MGIⁱ	MGI:97284 Ncf2

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000312227	1 – 525	Neutrophil cytosol factor 2Add BLAST		525

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	233	PhosphothreonineCombined sources	1
Modified residueⁱ	324	PhosphoserineCombined sources	1
Modified residueⁱ	398	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O70145
PaxDbⁱ	O70145
PeptideAtlas More...PeptideAtlasⁱ	O70145
PRIDEⁱ	O70145

PTM databases

iPTMnetⁱ	O70145
PhosphoSitePlusⁱ	O70145
SwissPalmⁱ	O70145

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000026480 Expressed in 27 organ(s), highest expression level in bone marrow macrophage
CleanExⁱ	MM_NCF2
ExpressionAtlasⁱ	O70145 baseline and differential
Genevisibleⁱ	O70145 MM

Interactionⁱ

Subunit structureⁱ

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Prdx6	O08709	3	EBI-9550667,EBI-444895

With

Entry

#Exp.

IntAct

Notes

Prdx6

O08709

3

EBI-9550667,EBI-444895

GO - Molecular functionⁱ

protein C-terminus binding Source: MGI
Rac GTPase binding
Source: MGI
Inferred from physical interactionⁱ
- 9490028

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	201702, 2 interactors
IntActⁱ	O70145, 2 interactors
STRINGⁱ	10090.ENSMUSP00000027754

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O70145
SMRⁱ	O70145
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	37 – 70	TPR 1Sequence analysisAdd BLAST	34
Repeatⁱ	71 – 104	TPR 2Sequence analysisAdd BLAST	34
Repeatⁱ	121 – 154	TPR 3Sequence analysisAdd BLAST	34
Domainⁱ	240 – 299	SH3 1PROSITE-ProRule annotationAdd BLAST	60
Domainⁱ	350 – 428	PB1PROSITE-ProRule annotationAdd BLAST	79
Domainⁱ	456 – 515	SH3 2PROSITE-ProRule annotationAdd BLAST	60

Domainⁱ

The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity

Sequence similaritiesⁱ

Belongs to the NCF2/NOXA1 family.Curated

Keywords - Domainⁱ

Repeat, SH3 domain, TPR repeat

Phylogenomic databases

eggNOGⁱ	KOG4225 Eukaryota ENOG41110AD LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063843
HOGENOMⁱ	HOG000237312
HOVERGENⁱ	HBG001521
InParanoidⁱ	O70145
KEGG Orthology (KO) More...KOⁱ	K08010
OMAⁱ	KLFRPNE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G04T7
PhylomeDBⁱ	O70145
TreeFamⁱ	TF329087

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06406 PB1_P67, 1 hit cd11871 SH3_p67phox_N, 1 hit
Gene3Dⁱ	1.25.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR034889 P67-PHOX IPR035546 p67phox_SH3_1 IPR000270 PB1_dom IPR034885 PB1_P67 IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR013026 TPR-contain_dom IPR011990 TPR-like_helical_dom_sf IPR001440 TPR_1 IPR019734 TPR_repeat
PANTHERⁱ	PTHR15175:SF3 PTHR15175:SF3, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00564 PB1, 1 hit PF00018 SH3_1, 2 hits PF00515 TPR_1, 1 hit PF13181 TPR_8, 1 hit
PRINTSⁱ	PR00452 SH3DOMAIN
SMARTⁱ	View protein in SMART SM00666 PB1, 1 hit SM00326 SH3, 2 hits SM00028 TPR, 3 hits
SUPFAMⁱ	SSF48452 SSF48452, 1 hit SSF50044 SSF50044, 2 hits
PROSITEⁱ	View protein in PROSITE PS51745 PB1, 1 hit PS50002 SH3, 2 hits PS50005 TPR, 3 hits PS50293 TPR_REGION, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

O70145-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSLAEAIRLW NEGVLAADKK DWKGALEAFS EVQDPHSRIC FNIGCVNTIL 
        60         70         80         90        100
ENLQAAEQAF TKSINRDKHS AVAYFQRGML YYRMEKYDLA IKDLKEALTQ 
       110        120        130        140        150
LRGNQLIDYK ILGLQFKLFA CEVLYNIALM HAKKEEWKKA EEQLALATNM 
       160        170        180        190        200
KSEPRHSKID KAMESIWKQK LFEPVVIPVG RLFRPNERQV AQLAKKDYLG 
       210        220        230        240        250
KATVVASVVH QDNFSGFAPL QPQSAEPPPR PKTPEIFRAL EGEAHRVLFG 
       260        270        280        290        300
FVPETPEELQ VMPGNIVFVL KKGSDNWATV MFNGQKGLVP CNYLEPVELR 
       310        320        330        340        350
IHPQSQPQED TSPESDIPPP PNSSPPGRLQ LSPGHKQKEP KELKLSVPMP 
       360        370        380        390        400
YMLKVHYKYT VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE 
       410        420        430        440        450
LLLLSEESMK DAWGQVKNYC LTLWCEHTVG DQGLIDEPIQ RENSDASKQT 
       460        470        480        490        500
TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG DVILVLSHVN EEWLEGECKG 
       510        520 
KVGIFPKAFV EGCAAKNLEG IPREV

Length:525

Mass (Da):59,485

Last modified:August 1, 1998 - v1

Checksum:ⁱ663DB6B52D790F76

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A087WPH0	A0A087WPH0_MOUSE		Neutrophil cytosol factor 2 Neutrophil cytosol factor 2	Ncf2	114	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	31	E → A in BAE42353 (PubMed:16141072).Curated	1
Sequence conflictⁱ	31	E → A in BAE42218 (PubMed:16141072).Curated	1
Sequence conflictⁱ	31	E → A in BAE42065 (PubMed:16141072).Curated	1
Sequence conflictⁱ	31	E → A in BAE33191 (PubMed:16141072).Curated	1
Sequence conflictⁱ	324	S → N in BAE32002 (PubMed:16141072).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB002664 mRNA Translation: BAA25650.1 AK036379 mRNA Translation: BAC29404.1 AK137152 mRNA Translation: BAE23254.1 AK153447 mRNA Translation: BAE32002.1 AK155324 mRNA Translation: BAE33191.1 AK170839 mRNA Translation: BAE42065.1 AK171059 mRNA Translation: BAE42218.1 AK171265 mRNA Translation: BAE42353.1 BC003730 mRNA Translation: AAH03730.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS15367.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035007.1, NM_010877.5 XP_006529299.1, XM_006529236.3
UniGeneⁱ	Mm.270307 Mm.489200

Genome annotation databases

Ensemblⁱ	ENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480 ENSMUST00000186568; ENSMUSP00000140404; ENSMUSG00000026480
GeneIDⁱ	17970
KEGGⁱ	mmu:17970
UCSC genome browser More...UCSCⁱ	uc007czm.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O70145	Neutrophil cytosolic factor 2		RAT		526	UniRef90_O70145
neutrophil cytosol factor 2		MESAU		526
Neutrophil cytosolic factor 2		MUSMM		525
Uncharacterized protein		NEOLE		524
Neutrophil cytosol factor 2 (Fragment)		MOUSE		114
+8

Protein	Similar proteins		Species	Score	Length	Source
O70145	Neutrophil cytosol factor 2	)	HUMAN		526	UniRef50_P19878
Neutrophil cytosol factor 2		RAT		527
Neutrophil cytosolic factor 2		GORGO		526
NCF2 isoform 3		PONAB		526
Uncharacterized protein		MACMU		526
+152

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB002664 mRNA Translation: BAA25650.1 AK036379 mRNA Translation: BAC29404.1 AK137152 mRNA Translation: BAE23254.1 AK153447 mRNA Translation: BAE32002.1 AK155324 mRNA Translation: BAE33191.1 AK170839 mRNA Translation: BAE42065.1 AK171059 mRNA Translation: BAE42218.1 AK171265 mRNA Translation: BAE42353.1 BC003730 mRNA Translation: AAH03730.1
CCDSⁱ	CCDS15367.1
RefSeqⁱ	NP_035007.1, NM_010877.5 XP_006529299.1, XM_006529236.3
UniGeneⁱ	Mm.270307 Mm.489200

3D structure databases

ProteinModelPortalⁱ	O70145
SMRⁱ	O70145
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	201702, 2 interactors
IntActⁱ	O70145, 2 interactors
STRINGⁱ	10090.ENSMUSP00000027754

PTM databases

iPTMnetⁱ	O70145
PhosphoSitePlusⁱ	O70145
SwissPalmⁱ	O70145

Proteomic databases

MaxQBⁱ	O70145
PaxDbⁱ	O70145
PeptideAtlasⁱ	O70145
PRIDEⁱ	O70145

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480 ENSMUST00000186568; ENSMUSP00000140404; ENSMUSG00000026480
GeneIDⁱ	17970
KEGGⁱ	mmu:17970
UCSCⁱ	uc007czm.1 mouse

Organism-specific databases

CTDⁱ	4688
MGIⁱ	MGI:97284 Ncf2

Phylogenomic databases

eggNOGⁱ	KOG4225 Eukaryota ENOG41110AD LUCA
GeneTreeⁱ	ENSGT00530000063843
HOGENOMⁱ	HOG000237312
HOVERGENⁱ	HBG001521
InParanoidⁱ	O70145
KOⁱ	K08010
OMAⁱ	KLFRPNE
OrthoDBⁱ	EOG091G04T7
PhylomeDBⁱ	O70145
TreeFamⁱ	TF329087

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1222556 ROS, RNS production in phagocytes R-MMU-1236973 Cross-presentation of particulate exogenous antigens (phagosomes) R-MMU-3299685 Detoxification of Reactive Oxygen Species R-MMU-4420097 VEGFA-VEGFR2 Pathway R-MMU-5668599 RHO GTPases Activate NADPH Oxidases

Reactomeⁱ

R-MMU-1222556 ROS, RNS production in phagocytes
R-MMU-1236973 Cross-presentation of particulate exogenous antigens (phagosomes)
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5668599 RHO GTPases Activate NADPH Oxidases

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O70145
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000026480 Expressed in 27 organ(s), highest expression level in bone marrow macrophage
CleanExⁱ	MM_NCF2
ExpressionAtlasⁱ	O70145 baseline and differential
Genevisibleⁱ	O70145 MM

Family and domain databases

CDDⁱ	cd06406 PB1_P67, 1 hit cd11871 SH3_p67phox_N, 1 hit
Gene3Dⁱ	1.25.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR034889 P67-PHOX IPR035546 p67phox_SH3_1 IPR000270 PB1_dom IPR034885 PB1_P67 IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR013026 TPR-contain_dom IPR011990 TPR-like_helical_dom_sf IPR001440 TPR_1 IPR019734 TPR_repeat
PANTHERⁱ	PTHR15175:SF3 PTHR15175:SF3, 1 hit
Pfamⁱ	View protein in Pfam PF00564 PB1, 1 hit PF00018 SH3_1, 2 hits PF00515 TPR_1, 1 hit PF13181 TPR_8, 1 hit
PRINTSⁱ	PR00452 SH3DOMAIN
SMARTⁱ	View protein in SMART SM00666 PB1, 1 hit SM00326 SH3, 2 hits SM00028 TPR, 3 hits
SUPFAMⁱ	SSF48452 SSF48452, 1 hit SSF50044 SSF50044, 2 hits
PROSITEⁱ	View protein in PROSITE PS51745 PB1, 1 hit PS50002 SH3, 2 hits PS50005 TPR, 3 hits PS50293 TPR_REGION, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NCF2_MOUSE
Accessionⁱ	O70145Primary (citable) accession number: O70145 Secondary accession number(s): Q3TC92, Q3U5S4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
	Last sequence update:	August 1, 1998
	Last modified:	November 7, 2018
	This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O70145 (NCF2_MOUSE)

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Neutrophil cytosol factor 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Nucleus

Plasma Membrane

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ