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Protein

Neutrophil cytosol factor 2

Gene

Ncf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).By similarity

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-1222556 ROS, RNS production in phagocytes
R-MMU-1236973 Cross-presentation of particulate exogenous antigens (phagosomes)
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5668599 RHO GTPases Activate NADPH Oxidases

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil cytosol factor 2
Short name:
NCF-2
Alternative name(s):
67 kDa neutrophil oxidase factor
NADPH oxidase activator 2
Neutrophil NADPH oxidase factor 2
p67-phox
Gene namesi
Name:Ncf2Imported
Synonyms:Noxa2, P67phox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:97284 Ncf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003122271 – 525Neutrophil cytosol factor 2Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233PhosphothreonineCombined sources1
Modified residuei324PhosphoserineCombined sources1
Modified residuei398PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO70145
PaxDbiO70145
PeptideAtlasiO70145
PRIDEiO70145

PTM databases

iPTMnetiO70145
PhosphoSitePlusiO70145
SwissPalmiO70145

Expressioni

Gene expression databases

BgeeiENSMUSG00000026480 Expressed in 27 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_NCF2
ExpressionAtlasiO70145 baseline and differential
GenevisibleiO70145 MM

Interactioni

Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Prdx6O087093EBI-9550667,EBI-444895

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201702, 2 interactors
IntActiO70145, 2 interactors
STRINGi10090.ENSMUSP00000027754

Structurei

3D structure databases

ProteinModelPortaliO70145
SMRiO70145
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 70TPR 1Sequence analysisAdd BLAST34
Repeati71 – 104TPR 2Sequence analysisAdd BLAST34
Repeati121 – 154TPR 3Sequence analysisAdd BLAST34
Domaini240 – 299SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini350 – 428PB1PROSITE-ProRule annotationAdd BLAST79
Domaini456 – 515SH3 2PROSITE-ProRule annotationAdd BLAST60

Domaini

The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.By similarity

Sequence similaritiesi

Belongs to the NCF2/NOXA1 family.Curated

Keywords - Domaini

Repeat, SH3 domain, TPR repeat

Phylogenomic databases

eggNOGiKOG4225 Eukaryota
ENOG41110AD LUCA
GeneTreeiENSGT00530000063843
HOGENOMiHOG000237312
HOVERGENiHBG001521
InParanoidiO70145
KOiK08010
OMAiKLFRPNE
OrthoDBiEOG091G04T7
PhylomeDBiO70145
TreeFamiTF329087

Family and domain databases

CDDicd06406 PB1_P67, 1 hit
cd11871 SH3_p67phox_N, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR034889 P67-PHOX
IPR035546 p67phox_SH3_1
IPR000270 PB1_dom
IPR034885 PB1_P67
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PANTHERiPTHR15175:SF3 PTHR15175:SF3, 1 hit
PfamiView protein in Pfam
PF00564 PB1, 1 hit
PF00018 SH3_1, 2 hits
PF00515 TPR_1, 1 hit
PF13181 TPR_8, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00666 PB1, 1 hit
SM00326 SH3, 2 hits
SM00028 TPR, 3 hits
SUPFAMiSSF48452 SSF48452, 1 hit
SSF50044 SSF50044, 2 hits
PROSITEiView protein in PROSITE
PS51745 PB1, 1 hit
PS50002 SH3, 2 hits
PS50005 TPR, 3 hits
PS50293 TPR_REGION, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O70145-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLAEAIRLW NEGVLAADKK DWKGALEAFS EVQDPHSRIC FNIGCVNTIL
60 70 80 90 100
ENLQAAEQAF TKSINRDKHS AVAYFQRGML YYRMEKYDLA IKDLKEALTQ
110 120 130 140 150
LRGNQLIDYK ILGLQFKLFA CEVLYNIALM HAKKEEWKKA EEQLALATNM
160 170 180 190 200
KSEPRHSKID KAMESIWKQK LFEPVVIPVG RLFRPNERQV AQLAKKDYLG
210 220 230 240 250
KATVVASVVH QDNFSGFAPL QPQSAEPPPR PKTPEIFRAL EGEAHRVLFG
260 270 280 290 300
FVPETPEELQ VMPGNIVFVL KKGSDNWATV MFNGQKGLVP CNYLEPVELR
310 320 330 340 350
IHPQSQPQED TSPESDIPPP PNSSPPGRLQ LSPGHKQKEP KELKLSVPMP
360 370 380 390 400
YMLKVHYKYT VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE
410 420 430 440 450
LLLLSEESMK DAWGQVKNYC LTLWCEHTVG DQGLIDEPIQ RENSDASKQT
460 470 480 490 500
TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG DVILVLSHVN EEWLEGECKG
510 520
KVGIFPKAFV EGCAAKNLEG IPREV
Length:525
Mass (Da):59,485
Last modified:August 1, 1998 - v1
Checksum:i663DB6B52D790F76
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WPH0A0A087WPH0_MOUSE
Neutrophil cytosol factor 2
Ncf2
114Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31E → A in BAE42353 (PubMed:16141072).Curated1
Sequence conflicti31E → A in BAE42218 (PubMed:16141072).Curated1
Sequence conflicti31E → A in BAE42065 (PubMed:16141072).Curated1
Sequence conflicti31E → A in BAE33191 (PubMed:16141072).Curated1
Sequence conflicti324S → N in BAE32002 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002664 mRNA Translation: BAA25650.1
AK036379 mRNA Translation: BAC29404.1
AK137152 mRNA Translation: BAE23254.1
AK153447 mRNA Translation: BAE32002.1
AK155324 mRNA Translation: BAE33191.1
AK170839 mRNA Translation: BAE42065.1
AK171059 mRNA Translation: BAE42218.1
AK171265 mRNA Translation: BAE42353.1
BC003730 mRNA Translation: AAH03730.1
CCDSiCCDS15367.1
RefSeqiNP_035007.1, NM_010877.5
XP_006529299.1, XM_006529236.3
UniGeneiMm.270307
Mm.489200

Genome annotation databases

EnsembliENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480
ENSMUST00000186568; ENSMUSP00000140404; ENSMUSG00000026480
GeneIDi17970
KEGGimmu:17970
UCSCiuc007czm.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002664 mRNA Translation: BAA25650.1
AK036379 mRNA Translation: BAC29404.1
AK137152 mRNA Translation: BAE23254.1
AK153447 mRNA Translation: BAE32002.1
AK155324 mRNA Translation: BAE33191.1
AK170839 mRNA Translation: BAE42065.1
AK171059 mRNA Translation: BAE42218.1
AK171265 mRNA Translation: BAE42353.1
BC003730 mRNA Translation: AAH03730.1
CCDSiCCDS15367.1
RefSeqiNP_035007.1, NM_010877.5
XP_006529299.1, XM_006529236.3
UniGeneiMm.270307
Mm.489200

3D structure databases

ProteinModelPortaliO70145
SMRiO70145
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201702, 2 interactors
IntActiO70145, 2 interactors
STRINGi10090.ENSMUSP00000027754

PTM databases

iPTMnetiO70145
PhosphoSitePlusiO70145
SwissPalmiO70145

Proteomic databases

MaxQBiO70145
PaxDbiO70145
PeptideAtlasiO70145
PRIDEiO70145

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027754; ENSMUSP00000027754; ENSMUSG00000026480
ENSMUST00000186568; ENSMUSP00000140404; ENSMUSG00000026480
GeneIDi17970
KEGGimmu:17970
UCSCiuc007czm.1 mouse

Organism-specific databases

CTDi4688
MGIiMGI:97284 Ncf2

Phylogenomic databases

eggNOGiKOG4225 Eukaryota
ENOG41110AD LUCA
GeneTreeiENSGT00530000063843
HOGENOMiHOG000237312
HOVERGENiHBG001521
InParanoidiO70145
KOiK08010
OMAiKLFRPNE
OrthoDBiEOG091G04T7
PhylomeDBiO70145
TreeFamiTF329087

Enzyme and pathway databases

ReactomeiR-MMU-1222556 ROS, RNS production in phagocytes
R-MMU-1236973 Cross-presentation of particulate exogenous antigens (phagosomes)
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5668599 RHO GTPases Activate NADPH Oxidases

Miscellaneous databases

PROiPR:O70145
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026480 Expressed in 27 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_NCF2
ExpressionAtlasiO70145 baseline and differential
GenevisibleiO70145 MM

Family and domain databases

CDDicd06406 PB1_P67, 1 hit
cd11871 SH3_p67phox_N, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR034889 P67-PHOX
IPR035546 p67phox_SH3_1
IPR000270 PB1_dom
IPR034885 PB1_P67
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PANTHERiPTHR15175:SF3 PTHR15175:SF3, 1 hit
PfamiView protein in Pfam
PF00564 PB1, 1 hit
PF00018 SH3_1, 2 hits
PF00515 TPR_1, 1 hit
PF13181 TPR_8, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00666 PB1, 1 hit
SM00326 SH3, 2 hits
SM00028 TPR, 3 hits
SUPFAMiSSF48452 SSF48452, 1 hit
SSF50044 SSF50044, 2 hits
PROSITEiView protein in PROSITE
PS51745 PB1, 1 hit
PS50002 SH3, 2 hits
PS50005 TPR, 3 hits
PS50293 TPR_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNCF2_MOUSE
AccessioniPrimary (citable) accession number: O70145
Secondary accession number(s): Q3TC92, Q3U5S4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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