UniProtKB - O69689 (ASPAT_MYCTU)
Protein
Aspartate aminotransferase
Gene
Rv3722c
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Main aspartate aminotransferase that couples nitrogen assimilation to aspartate synthesis. Has a weak, but significant, side activity toward kynurenine (Kyn). Oxaloacetate and 2-oxoglutarate, but not pyruvate, serve as amino acceptors, while Asp, Glu and Kyn serve as the best amino donors. Essential for axenic growth and survival of M.tuberculosis in macrophages and in mice.1 Publication
Catalytic activityi
- EC:2.6.1.11 Publication
Cofactori
pyridoxal 5'-phosphate1 Publication
Kineticsi
kcat is 136 sec(-1) with aspartate as substrate. kcat is 198 sec(-1) with kynurenine as substrate.1 Publication
- KM=2.34 mM for aspartate1 Publication
- KM=29.4 mM for kynurenine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 69 | Pyridoxal phosphate2 Publications | 1 | |
Binding sitei | 189 | Pyridoxal phosphate2 Publications | 1 | |
Binding sitei | 221 | Pyridoxal phosphate2 Publications | 1 | |
Binding sitei | 392 | Substrate1 Publication | 1 |
GO - Molecular functioni
- L-aspartate:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Aminotransferase, Transferase |
Ligand | Pyridoxal phosphate |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate aminotransferase1 Publication (EC:2.6.1.11 Publication)Short name: AspAT1 Publication |
Gene namesi | Ordered Locus Names:Rv3722cImported |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3722c |
Pathology & Biotechi
Disruption phenotypei
Deletion of the gene leads to virulence attenuation in macrophages and mice.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000450667 | 1 – 435 | Aspartate aminotransferaseAdd BLAST | 435 |
Proteomic databases
PaxDbi | O69689 |
PRIDEi | O69689 |
Interactioni
Protein-protein interaction databases
IntActi | O69689, 12 interactors |
STRINGi | 83332.Rv3722c |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O69689 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 100 – 101 | Pyridoxal phosphate binding2 Publications | 2 | |
Regioni | 139 – 141 | Substrate binding1 Publication | 3 | |
Regioni | 254 – 256 | Pyridoxal phosphate binding2 Publications | 3 |
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | COG1167, Bacteria |
OMAi | AMDGVAT |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR024551, AspAT_Ic IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF12897, Aminotran_MocR, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
i Sequence
Sequence statusi: Complete.
O69689-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFDSLSPQE LAALHARHQQ DYAALQGMKL ALDLTRGKPS AEQLDLSNQL
60 70 80 90 100
LSLPGDDYRD PEGTDTRNYG GQHGLPGLRA IFAELLGIAV PNLIAGNNSS
110 120 130 140 150
LELMHDIVAF SMLYGGVDSP RPWIQEQDGI KFLCPVPGYD RHFAITETMG
160 170 180 190 200
IEMIPIPMLQ DGPDVDLIEE LVAVDPAIKG MWTVPVFGNP SGVTYSWETV
210 220 230 240 250
RRLVQMRTAA PDFRLFWDNA YAVHTLTLDF PRQVDVLGLA AKAGNPNRPY
260 270 280 290 300
VFASTSKITF AGGGVSFFGG SLGNIAWYLQ YAGKKSIGPD KVNQLRHLRF
310 320 330 340 350
FGDADGVRLH MLRHQQILAP KFALVAEVLD QRLSESKIAS WTEPKGGYFI
360 370 380 390 400
SLDVLPGTAR RTVALAKDVG IAVTEAGASF PYRKDPDDKN IRIAPSFPSV
410 420 430
PDLRNAVDGL ATCALLAATE TLLNQGLASS APNVR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46548.1 |
RefSeqi | NP_218239.2, NC_000962.3 WP_003899653.1, NZ_NVQJ01000009.1 |
Genome annotation databases
EnsemblBacteriai | AIR16512; AIR16512; LH57_20280 CCP46548; CCP46548; Rv3722c |
GeneIDi | 885321 |
KEGGi | mtu:Rv3722c |
PATRICi | fig|83332.111.peg.4138 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46548.1 |
RefSeqi | NP_218239.2, NC_000962.3 WP_003899653.1, NZ_NVQJ01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5C6U | X-ray | 1.83 | A | 3-430 | [»] | |
6U78 | X-ray | 2.60 | A/B/C/D | 1-435 | [»] | |
6U7A | X-ray | 2.22 | A/B/C/D/E/F/G/H | 1-435 | [»] | |
SMRi | O69689 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | O69689, 12 interactors |
STRINGi | 83332.Rv3722c |
Proteomic databases
PaxDbi | O69689 |
PRIDEi | O69689 |
Genome annotation databases
EnsemblBacteriai | AIR16512; AIR16512; LH57_20280 CCP46548; CCP46548; Rv3722c |
GeneIDi | 885321 |
KEGGi | mtu:Rv3722c |
PATRICi | fig|83332.111.peg.4138 |
Organism-specific databases
TubercuListi | Rv3722c |
Phylogenomic databases
eggNOGi | COG1167, Bacteria |
OMAi | AMDGVAT |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR024551, AspAT_Ic IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF12897, Aminotran_MocR, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASPAT_MYCTU | |
Accessioni | O69689Primary (citable) accession number: O69689 Secondary accession number(s): F2GFU4, I6Y4D4, Q7D501 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 12, 2020 |
Last sequence update: | March 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families