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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygenUniRule annotation1
Binding sitei208UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei216UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei410Meso-diaminopimelateUniRule annotation1
Binding sitei488Meso-diaminopimelate; via carbonyl oxygenUniRule annotation1
Binding sitei492Meso-diaminopimelateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi139 – 145ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-986-MONOMER
BRENDAi6.3.2.13 3504
UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotation
Ordered Locus Names:ML0909
ORF Names:MLCB268.07c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0909

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001019121 – 530UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei248N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Proteomic databases

PRIDEiO69557

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0909

Structurei

3D structure databases

ProteinModelPortaliO69557
SMRiO69557
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 182UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation2
Regioni434 – 437Meso-diaminopimelate bindingUniRule annotation4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi434 – 437Meso-diaminopimelate recognition motif4

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EEN Bacteria
COG0769 LUCA
HOGENOMiHOG000268118
KOiK01928
OMAiCFMEVSS
OrthoDBiPOG091H0082

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00208 MurE, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR000713 Mur_ligase_N
IPR035911 MurE/MurF_N
IPR005761 UDP-N-AcMur-Glu-dNH2Pim_ligase
PfamiView protein in Pfam
PF01225 Mur_ligase, 1 hit
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
SSF63418 SSF63418, 1 hit
TIGRFAMsiTIGR01085 murE, 1 hit

Sequencei

Sequence statusi: Complete.

O69557-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTEVVAVPVR LRPSATAGVR LPELVAQVGA VLADGPGQAA TVPDIPVTGV
60 70 80 90 100
TLRAQEVLSG DLFAALAGAS THGARYAGVA LERGAVAVLT DVAGVAELTA
110 120 130 140 150
QASSVPILIH PEPRSVLGGL AAAAYGHPSN RMTVVGITGT SGKTTTTYMV
160 170 180 190 200
EAGLRAGGRV VGLVGTIGIR IDGADIPSFL TTPEAPALQA MLAAMVERGV
210 220 230 240 250
ETVVMEVSSH ALSLGRVEGT QFAVAGFTNL SRDHLDFHPD MEEYFEAKAV
260 270 280 290 300
LFDPHSLLRA RTVVVCIDDD AGRATAARAG DAITVSALGQ PASWRATDIR
310 320 330 340 350
SPGVGAQEFT AVDPAGVQHR VGIRLPGRYN VANCLVALAI LDVVGVSPEQ
360 370 380 390 400
ASLGFRDIRI PGRLERIDCG QDFLALVDYA HKPGALHSVL TALLQPDHRL
410 420 430 440 450
AVVFGAGGER DPGKRAPMGE IAAELADLVV VTDDNPRGED PAAIRRDILT
460 470 480 490 500
GTVAAGGAAQ VVEIGDRRAA IQYAVAWAGP DDVVLVAGKG HETGQRGAAE
510 520 530
TCPFDDRVEL ARALQVRDAR LLPAPGRACQ
Length:530
Mass (Da):54,808
Last modified:August 1, 1998 - v1
Checksum:iD042925C10E1F2FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022602 Genomic DNA Translation: CAA18673.1
AL583920 Genomic DNA Translation: CAC31290.1
PIRiG87022
RefSeqiNP_301692.1, NC_002677.1
WP_010908016.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC31290; CAC31290; CAC31290
GeneIDi909910
KEGGimle:ML0909
PATRICifig|272631.5.peg.1652

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022602 Genomic DNA Translation: CAA18673.1
AL583920 Genomic DNA Translation: CAC31290.1
PIRiG87022
RefSeqiNP_301692.1, NC_002677.1
WP_010908016.1, NC_002677.1

3D structure databases

ProteinModelPortaliO69557
SMRiO69557
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0909

Proteomic databases

PRIDEiO69557

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31290; CAC31290; CAC31290
GeneIDi909910
KEGGimle:ML0909
PATRICifig|272631.5.peg.1652

Organism-specific databases

LepromaiML0909

Phylogenomic databases

eggNOGiENOG4107EEN Bacteria
COG0769 LUCA
HOGENOMiHOG000268118
KOiK01928
OMAiCFMEVSS
OrthoDBiPOG091H0082

Enzyme and pathway databases

UniPathwayi
UPA00219

BioCyciMLEP272631:G1GT5-986-MONOMER
BRENDAi6.3.2.13 3504

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00208 MurE, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR000713 Mur_ligase_N
IPR035911 MurE/MurF_N
IPR005761 UDP-N-AcMur-Glu-dNH2Pim_ligase
PfamiView protein in Pfam
PF01225 Mur_ligase, 1 hit
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
SSF63418 SSF63418, 1 hit
TIGRFAMsiTIGR01085 murE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMURE_MYCLE
AccessioniPrimary (citable) accession number: O69557
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: October 10, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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