UniProtKB - O69078 (CSRA_PSEAE)
Translational regulator CsrA
csrA
Functioni
A key translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability. Mediates global changes in gene expression, shifting from rapid growth to stress survival by linking envelope stress, the stringent response and the catabolite repression systems. Usually binds in the 5'-UTR; binding at or near the Shine-Dalgarno sequence prevents ribosome-binding, repressing translation, binding elsewhere in the 5'-UTR can activate translation and/or stabilize the mRNA. Its function is antagonized by small RNA(s).
UniRule annotationBinds to mRNA to regulate gene activity at a post-transcriptional level (Probable). Represses expression of many toxic extracellular enzymes and compounds; decreases translation of lasI and rhlI (PubMed:11673439).
Positively controls swarming motility and rhanolipid and lipase, possibly via expression of rhlA; activates transcription of the CsrA/RsmA antagonistic sRNA RsmZ (PubMed:15126453).
Overexpression dramatically reduces extracellular protease, elastase (lasB) and staphyolytic (lasA) activities, decreases HCN production, decreases levels of autoinducers 3-oxo-C12-HSL (3-oxo-N-(tetrahydro-2-oxo-3-furanyl)-dodecanamide) and C4-HSL (N-butanoylhomoserine lactone), and abolishes production of cytotoxic internal lectin PA-IL (lecA) (PubMed:11673439).
Control of hcn expression is post-transcriptional (PubMed:11673439).
Replaces endogenous gene(s) in E.coli and P.fluorescens (PubMed:16359708).
3 PublicationsProbably binds to and is sequestered by non-coding small RNA (sRNA) RsmZ; overexpression of rsmZ produces very similar phenotypes to deletion of rsmA, while rsmZ deletion has no phenotype (PubMed:15126453).
1 PublicationGO - Molecular functioni
- mRNA 5'-UTR binding Source: GO_Central
GO - Biological processi
- mRNA catabolic process Source: InterPro
- negative regulation of translational initiation Source: GO_Central
- positive regulation of translational initiation Source: UniProtKB-UniRule
- protein secretion by the type III secretion system Source: PseudoCAP
- protein secretion by the type VI secretion system Source: PseudoCAP
- quorum sensing Source: PseudoCAP
- regulation of carbohydrate metabolic process Source: UniProtKB-UniRule
- regulation of secondary metabolic process Source: PseudoCAP
- regulation of single-species biofilm formation Source: PseudoCAP
Keywordsi
Molecular function | Activator, Repressor, RNA-binding |
Biological process | Translation regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Translational regulator CsrAUniRule annotationAlternative name(s): Carbon storage regulatorUniRule annotation Global translational regulatory protein RsmA1 Publication |
Gene namesi | Name:csrAUniRule annotation Synonyms:rsmA1 Publication Ordered Locus Names:PA0905 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA0905 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | E → A: Behaves like wild-type in swarming, pyocyanine production and lipase biosynthesis assays. 1 Publication | 1 | |
Mutagenesisi | 44 | R → A: Does not complement deletion in swarming, pyocyanine production or lipase biosynthesis assays. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000177080 | 1 – 61 | Translational regulator CsrAAdd BLAST | 61 |
Proteomic databases
PaxDbi | O69078 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Homodimer; the beta-strands of each monomer intercalate to form a hydrophobic core while the alpha-helices form wings that extend away from the core (PubMed:16104018).
UniRule annotation1 PublicationProtein-protein interaction databases
STRINGi | 287.DR97_1038 |
Structurei
Secondary structure
3D structure databases
SMRi | O69078 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O69078 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_164837_2_1_6 |
InParanoidi | O69078 |
OMAi | IHRKEVY |
PhylomeDBi | O69078 |
Family and domain databases
Gene3Di | 2.60.40.4380, 1 hit |
HAMAPi | MF_00167, CsrA, 1 hit |
InterProi | View protein in InterPro IPR003751, CsrA IPR036107, CsrA_sf |
PANTHERi | PTHR34984, PTHR34984, 1 hit |
Pfami | View protein in Pfam PF02599, CsrA, 1 hit |
SUPFAMi | SSF117130, SSF117130, 1 hit |
TIGRFAMsi | TIGR00202, csrA, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLILTRRVGE TLMVGDDVTV TVLGVKGNQV RIGVNAPKEV AVHREEIYQR
60
IQKEKDQEPN H
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061757 Genomic DNA Translation: AAC16242.1 AE004091 Genomic DNA Translation: AAG04294.1 |
PIRi | D83531 |
RefSeqi | NP_249596.1, NC_002516.2 WP_003085981.1, NZ_QZGE01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAG04294; AAG04294; PA0905 |
GeneIDi | 61675155 878352 |
KEGGi | pae:PA0905 |
PATRICi | fig|208964.12.peg.940 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061757 Genomic DNA Translation: AAC16242.1 AE004091 Genomic DNA Translation: AAG04294.1 |
PIRi | D83531 |
RefSeqi | NP_249596.1, NC_002516.2 WP_003085981.1, NZ_QZGE01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1VPZ | X-ray | 2.05 | A/B | 1-61 | [»] | |
SMRi | O69078 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 287.DR97_1038 |
Proteomic databases
PaxDbi | O69078 |
Protocols and materials databases
DNASUi | 878352 |
Genome annotation databases
EnsemblBacteriai | AAG04294; AAG04294; PA0905 |
GeneIDi | 61675155 878352 |
KEGGi | pae:PA0905 |
PATRICi | fig|208964.12.peg.940 |
Organism-specific databases
PseudoCAPi | PA0905 |
Phylogenomic databases
HOGENOMi | CLU_164837_2_1_6 |
InParanoidi | O69078 |
OMAi | IHRKEVY |
PhylomeDBi | O69078 |
Miscellaneous databases
EvolutionaryTracei | O69078 |
Family and domain databases
Gene3Di | 2.60.40.4380, 1 hit |
HAMAPi | MF_00167, CsrA, 1 hit |
InterProi | View protein in InterPro IPR003751, CsrA IPR036107, CsrA_sf |
PANTHERi | PTHR34984, PTHR34984, 1 hit |
Pfami | View protein in Pfam PF02599, CsrA, 1 hit |
SUPFAMi | SSF117130, SSF117130, 1 hit |
TIGRFAMsi | TIGR00202, csrA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CSRA_PSEAE | |
Accessioni | O69078Primary (citable) accession number: O69078 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families