UniProtKB - O68953 (VNFD_AZOPA)
Protein
Nitrogenase vanadium-iron protein alpha chain
Gene
vnfD
Organism
Azorhizophilus paspali (Azotobacter paspali)
Status
Functioni
This vanadium-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.
Miscellaneous
The structure of the 7Fe-V-9S-C-homocitryl cluster is assumed to be analogous to the 7Fe-Mo-9S-C-homocitryl cluster.
Catalytic activityi
- 16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H+ + H2 + 2 NH4+ + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphateEC:1.18.6.1
Cofactori
Protein has several cofactor binding sites:- [8Fe-7S] clusterBy similarityNote: Binds 1 [8Fe-7S] cluster per heterodimer.By similarity
- [7Fe-V-9S-C-homocitryl] clusterBy similarityNote: Binds 1 [7Fe-V-9S-C-homocitryl] cluster per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 17 | Iron-sulfur (8Fe-7S); shared with beta chainBy similarity | 1 | |
Metal bindingi | 80 | Iron-sulfur (8Fe-7S); shared with beta chainBy similarity | 1 | |
Metal bindingi | 199 | Vanadium-iron-sulfur-carbon (7Fe-V-9S-C-homocitryl)By similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- carbonyl sulfide nitrogenase activity Source: UniProtKB-EC
- iron-sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- nitrogenase activity Source: UniProtKB-EC
GO - Biological processi
- nitrogen fixation Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Nitrogen fixation |
Ligand | ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, Vanadium |
Names & Taxonomyi
Protein namesi | Recommended name: Nitrogenase vanadium-iron protein alpha chain (EC:1.18.6.1)Alternative name(s): Dinitrogenase 2 subunit alpha Nitrogenase component I |
Gene namesi | Name:vnfD |
Organismi | Azorhizophilus paspali (Azotobacter paspali) |
Taxonomic identifieri | 69963 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azorhizophilus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000153056 | ‹1 – ›265 | Nitrogenase vanadium-iron protein alpha chainAdd BLAST | ›265 |
Interactioni
Subunit structurei
Hexamer of two alpha, two beta, and two delta chains.
By similarityFamily & Domainsi
Sequence similaritiesi
Belongs to the NifD/NifK/NifE/NifN family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR000510, Nase/OxRdtase_comp1 IPR005974, Nase_asu IPR010143, Nase_comp1_asu IPR000318, Nase_comp1_CS |
PANTHERi | PTHR43457, PTHR43457, 1 hit |
Pfami | View protein in Pfam PF00148, Oxidored_nitro, 1 hit |
TIGRFAMsi | TIGR01284, alt_nitrog_alph, 1 hit |
PROSITEi | View protein in PROSITE PS00699, NITROGENASE_1_1, 1 hit PS00090, NITROGENASE_1_2, 1 hit |
i Sequence
Sequence statusi: Fragment.
O68953-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
GGVLKDTIQM IHGPLGCAYD TWHTKRYPTD NGHFNMKYVW STDMKESHVV
60 70 80 90 100
FGGEKRLEKS MHEAFDEMPD IKRMIVYTTC PTALIGDDIK AVAKKVMKDR
110 120 130 140 150
PDVDVFTVEC PGFSGVSQSK GHHVLNIGWI NEKVETMEKE ITSEYTMNFI
160 170 180 190 200
GDFNIQGDTQ LLQTYWDRLG IQVVAHFTGN GTYDDLRCMH QAQLNVVNCA
210 220 230 240 250
RSSGYIANEL KKRYGIPRLD IDSWGFNYMA EGIRKICAFF GIEEKGEELI
260
AEEYAKWKPK LDWYK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 | ||
Non-terminal residuei | 265 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF058783 Genomic DNA Translation: AAC14340.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF058783 Genomic DNA Translation: AAC14340.1 |
3D structure databases
SMRi | O68953 |
ModBasei | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR000510, Nase/OxRdtase_comp1 IPR005974, Nase_asu IPR010143, Nase_comp1_asu IPR000318, Nase_comp1_CS |
PANTHERi | PTHR43457, PTHR43457, 1 hit |
Pfami | View protein in Pfam PF00148, Oxidored_nitro, 1 hit |
TIGRFAMsi | TIGR01284, alt_nitrog_alph, 1 hit |
PROSITEi | View protein in PROSITE PS00699, NITROGENASE_1_1, 1 hit PS00090, NITROGENASE_1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VNFD_AZOPA | |
Accessioni | O68953Primary (citable) accession number: O68953 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | August 1, 1998 | |
Last modified: | October 7, 2020 | |
This is version 73 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |