UniProtKB - O68106 (CBIO_RHOCB)
Protein
Cobalt import ATP-binding protein CbiO
Gene
cbiO
Organism
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Status
Functioni
Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import. The complex confers cobalt uptake upon expression in E.coli; can also transport nickel with a very low affinity. Presumably responsible for energy coupling to the transport system.2 Publications
: adenosylcobalamin biosynthesis Pathwayi
This protein is involved in the pathway adenosylcobalamin biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 38 – 45 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- cobalamin biosynthetic process Source: UniProtKB-UniPathway
- cobalt ion transport Source: UniProtKB
Keywordsi
Molecular function | Translocase |
Biological process | Cobalamin biosynthesis, Cobalt transport, Ion transport, Transport |
Ligand | ATP-binding, Cobalt, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | RCAP272942:G1GUE-2039-MONOMER |
UniPathwayi | UPA00148 |
Protein family/group databases
TCDBi | 3.A.1.23.8, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cobalt import ATP-binding protein CbiO (EC:7.2.2.-)Alternative name(s): Energy-coupling factor transporter ATP-binding protein CbiO Short name: ECF transporter A component CbiO |
Gene namesi | Name:cbiO Synonyms:cbiO2 Ordered Locus Names:RCAP_rcc02034 |
Organismi | Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) |
Taxonomic identifieri | 272942 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane By similarity; Peripheral membrane protein By similarity
GO - Cellular componenti
- ATP-binding cassette (ABC) transporter complex Source: UniProtKB
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092057 | 1 – 280 | Cobalt import ATP-binding protein CbiOAdd BLAST | 280 |
Interactioni
Subunit structurei
Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry. Subcomplexes composed of CbiMQO can be isolated from membranes but the CbiN subunit is not isolated in association with them, suggesting it is only loosely associated. Expression of just CbiMN in E.coli confers some cobalt uptake.
2 PublicationsProtein-protein interaction databases
STRINGi | 272942.RCAP_rcc02034 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O68106 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 240 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 236 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily.Curated
Phylogenomic databases
eggNOGi | COG1122, Bacteria |
HOGENOMi | CLU_000604_1_22_5 |
OMAi | LQDPDDQ |
OrthoDBi | 1752365at2 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR005876, Co_trans_ATP-bd IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01166, cbiO, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O68106-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTPILAAEAL TYAFPGGVKA LDDLSLAVPQ GESLAILGPN GAGKSTLLLH
60 70 80 90 100
LNGTLRPQSG RVLLGGTATG HSRKDLTDWR RRVGLVLQDA DDQLFAATVF
110 120 130 140 150
EDVSFGPLNL GLSEAEARAR VEEALAALSI SDLRDRPTHM LSGGQKRRVA
160 170 180 190 200
IAGAVAMRPE VLLLDEPTAG LDLAGTEQLL TLLHGLRAAG MTLVFSTHDV
210 220 230 240 250
ELAAALADRV ALFRTGRVLA EGAAAAVLSD RATLAQGGLR PPLVIDLALS
260 270 280
RARSRPFGPR SALPRTRDAL AAQMAGWTRR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF010496 Genomic DNA Translation: AAC16196.1 CP001312 Genomic DNA Translation: ADE85778.1 |
PIRi | T03543 |
RefSeqi | WP_013067757.1, NC_014034.1 |
Genome annotation databases
EnsemblBacteriai | ADE85778; ADE85778; RCAP_rcc02034 |
KEGGi | rcp:RCAP_rcc02034 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF010496 Genomic DNA Translation: AAC16196.1 CP001312 Genomic DNA Translation: ADE85778.1 |
PIRi | T03543 |
RefSeqi | WP_013067757.1, NC_014034.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5X3X | X-ray | 2.79 | A/B/a/b | 1-280 | [»] | |
5X40 | X-ray | 1.45 | A/B | 1-280 | [»] | |
SMRi | O68106 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 272942.RCAP_rcc02034 |
Protein family/group databases
TCDBi | 3.A.1.23.8, the atp-binding cassette (abc) superfamily |
Genome annotation databases
EnsemblBacteriai | ADE85778; ADE85778; RCAP_rcc02034 |
KEGGi | rcp:RCAP_rcc02034 |
Phylogenomic databases
eggNOGi | COG1122, Bacteria |
HOGENOMi | CLU_000604_1_22_5 |
OMAi | LQDPDDQ |
OrthoDBi | 1752365at2 |
Enzyme and pathway databases
UniPathwayi | UPA00148 |
BioCyci | RCAP272942:G1GUE-2039-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR005876, Co_trans_ATP-bd IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01166, cbiO, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CBIO_RHOCB | |
Accessioni | O68106Primary (citable) accession number: O68106 Secondary accession number(s): D5AUZ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 21, 2004 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families