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UniProtKB - O67625 (ISPH_AQUAE)
Protein
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Gene
ispH
Organism
Aquifex aeolicus (strain VF5)
Status
Functioni
Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.
UniRule annotation1 PublicationCatalytic activityi
- H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + 2 reduced [2Fe-2S]-[ferredoxin]UniRule annotation1 PublicationEC:1.17.7.4UniRule annotation1 Publication
- dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + 2 reduced [2Fe-2S]-[ferredoxin]UniRule annotation1 PublicationEC:1.17.7.4UniRule annotation1 Publication
Cofactori
[4Fe-4S] clusterUniRule annotationNote: Was shown to bind 1 [3Fe-4S] cluster per subunit (PubMed:19035630). However, it likely initially contains a [4Fe-4S] cluster which easily degrades into a [3Fe-4S] form in the presence of oxygen (By similarity).UniRule annotationBy similarity1 Publication
Activity regulationi
Highly sensitive to dioxygen.1 Publication
Kineticsi
- KM=590 µM for 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate1 Publication
pH dependencei
Optimum pH is 7.0-7.5.1 Publication
Temperature dependencei
Optimum temperature is 60 degrees Celsius.1 Publication
: dimethylallyl diphosphate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.
Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway
This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 13 | Iron-sulfur (4Fe-4S)UniRule annotation1 Publication | 1 | |
Binding sitei | 42 | SubstrateUniRule annotation | 1 | |
Binding sitei | 74 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 96 | Iron-sulfur (4Fe-4S)UniRule annotation1 Publication | 1 | |
Binding sitei | 124 | SubstrateUniRule annotation | 1 | |
Active sitei | 126 | Proton donorUniRule annotation | 1 | |
Binding sitei | 165 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 193 | Iron-sulfur (4Fe-4S)UniRule annotation1 Publication | 1 | |
Binding sitei | 265 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Source: UniProtKB-UniRule
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- dimethylallyl diphosphate biosynthetic process Source: UniProtKB-UniRule
- isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniRule
- terpenoid biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Isoprene biosynthesis |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | AAEO224324:G1G15-1238-MONOMER |
BRENDAi | 1.17.7.4, 396 |
UniPathwayi | UPA00056;UER00097 UPA00059;UER00105 |
Names & Taxonomyi
Protein namesi | Recommended name: 4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation1 Publication)Short name: HMBPP reductaseUniRule annotation |
Gene namesi | Name:ispHUniRule annotation Synonyms:lytB Ordered Locus Names:aq_1739 |
Organismi | Aquifex aeolicus (strain VF5) |
Taxonomic identifieri | 224324 [NCBI] |
Taxonomic lineagei | Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000128766 | 1 – 289 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductaseAdd BLAST | 289 |
Interactioni
Protein-protein interaction databases
STRINGi | 224324.aq_1739 |
Chemistry databases
BindingDBi | O67625 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O67625 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O67625 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 221 – 223 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the IspH family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0761, Bacteria |
HOGENOMi | CLU_027486_0_1_0 |
InParanoidi | O67625 |
OMAi | HNKYVVD |
OrthoDBi | 1235756at2 |
Family and domain databases
CDDi | cd13944, lytB_ispH, 1 hit |
HAMAPi | MF_00191, IspH, 1 hit |
InterProi | View protein in InterPro IPR003451, LytB/IspH |
PANTHERi | PTHR30426, PTHR30426, 1 hit |
Pfami | View protein in Pfam PF02401, LYTB, 1 hit |
TIGRFAMsi | TIGR00216, ispH_lytB, 1 hit |
i Sequence
Sequence statusi: Complete.
O67625-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVDIIIAEHA GFCFGVKRAV KLAEESLKES QGKVYTLGPI IHNPQEVNRL
60 70 80 90 100
KNLGVFPSQG EEFKEGDTVI IRSHGIPPEK EEALRKKGLK VIDATCPYVK
110 120 130 140 150
AVHEAVCQLT REGYFVVLVG EKNHPEVIGT LGYLRACNGK GIVVETLEDI
160 170 180 190 200
GEALKHERVG IVAQTTQNEE FFKEVVGEIA LWVKEVKVIN TICNATSLRQ
210 220 230 240 250
ESVKKLAPEV DVMIIIGGKN SGNTRRLYYI SKELNPNTYH IETAEELQPE
260 270 280
WFRGVKRVGI SAGASTPDWI IEQVKSRIQE ICEGQLVSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC07596.1 |
PIRi | G70449 |
RefSeqi | NP_214191.1, NC_000918.1 WP_010881128.1, NC_000918.1 |
Genome annotation databases
EnsemblBacteriai | AAC07596; AAC07596; aq_1739 |
KEGGi | aae:aq_1739 |
PATRICi | fig|224324.8.peg.1338 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC07596.1 |
PIRi | G70449 |
RefSeqi | NP_214191.1, NC_000918.1 WP_010881128.1, NC_000918.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3DNF | X-ray | 1.65 | A/B | 1-289 | [»] | |
SMRi | O67625 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224324.aq_1739 |
Chemistry databases
BindingDBi | O67625 |
ChEMBLi | CHEMBL2242741 |
Genome annotation databases
EnsemblBacteriai | AAC07596; AAC07596; aq_1739 |
KEGGi | aae:aq_1739 |
PATRICi | fig|224324.8.peg.1338 |
Phylogenomic databases
eggNOGi | COG0761, Bacteria |
HOGENOMi | CLU_027486_0_1_0 |
InParanoidi | O67625 |
OMAi | HNKYVVD |
OrthoDBi | 1235756at2 |
Enzyme and pathway databases
UniPathwayi | UPA00056;UER00097 UPA00059;UER00105 |
BioCyci | AAEO224324:G1G15-1238-MONOMER |
BRENDAi | 1.17.7.4, 396 |
Miscellaneous databases
EvolutionaryTracei | O67625 |
Family and domain databases
CDDi | cd13944, lytB_ispH, 1 hit |
HAMAPi | MF_00191, IspH, 1 hit |
InterProi | View protein in InterPro IPR003451, LytB/IspH |
PANTHERi | PTHR30426, PTHR30426, 1 hit |
Pfami | View protein in Pfam PF02401, LYTB, 1 hit |
TIGRFAMsi | TIGR00216, ispH_lytB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ISPH_AQUAE | |
Accessioni | O67625Primary (citable) accession number: O67625 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | August 1, 1998 | |
Last modified: | June 2, 2021 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families