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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NAD; via carbonyl oxygenBy similarity1
Binding sitei92NAD; via carbonyl oxygenBy similarity1
Binding sitei95Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei146Proton acceptorBy similarity1
Active sitei156Proton acceptorBy similarity1
Binding sitei163NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 20NADBy similarity2
Nucleotide bindingi64 – 65NADBy similarity2
Nucleotide bindingi192 – 196NADBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00094

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:aq_1552
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000548941 – 270Enoyl-[acyl-carrier-protein] reductase [NADH] FabIAdd BLAST270

Proteomic databases

PRIDEiO67505

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi224324.aq_1552

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni3 – 6Combined sources4
Beta strandi8 – 11Combined sources4
Helixi20 – 30Combined sources11
Beta strandi34 – 41Combined sources8
Helixi42 – 44Combined sources3
Helixi45 – 54Combined sources10
Beta strandi60 – 62Combined sources3
Helixi68 – 81Combined sources14
Beta strandi87 – 90Combined sources4
Helixi97 – 100Combined sources4
Helixi104 – 106Combined sources3
Helixi109 – 119Combined sources11
Helixi121 – 130Combined sources10
Helixi131 – 134Combined sources4
Beta strandi140 – 145Combined sources6
Helixi147 – 149Combined sources3
Turni154 – 157Combined sources4
Helixi158 – 178Combined sources21
Turni179 – 181Combined sources3
Beta strandi183 – 189Combined sources7
Helixi203 – 213Combined sources11
Helixi222 – 232Combined sources11
Helixi235 – 237Combined sources3
Beta strandi244 – 248Combined sources5
Helixi251 – 253Combined sources3

3D structure databases

ProteinModelPortaliO67505
SMRiO67505
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO67505

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CSJ Bacteria
COG0623 LUCA
InParanoidiO67505
KOiK00208
OMAiGILDMIH
OrthoDBiPOG091H06HU

Family and domain databases

InterProiView protein in InterPro
IPR014358 Enoyl-ACP_Rdtase_NADH
IPR036291 NAD(P)-bd_dom_sf
IPR002347 SDR_fam
PANTHERiPTHR43159:SF2 PTHR43159:SF2, 1 hit
PIRSFiPIRSF000094 Enoyl-ACP_rdct, 1 hit
PRINTSiPR00081 GDHRDH
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

O67505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLEGKRAL ITGVANERSI AYGIAKSFHR EGAQLAFTYA TPKLEKRVRE
60 70 80 90 100
IAKGFGSDLV VKCDVSLDED IKNLKKFLEE NWGSLDIIVH SIAYAPKEEF
110 120 130 140 150
KGGVIDTSRE GFKIAMDISV YSLIALTREL LPLMEGRNGA IVTLSYYGAE
160 170 180 190 200
KVVPHYNVMG IAKAALESTV RYLAYDIAKH GHRINAISAG PVKTLAAYSI
210 220 230 240 250
TGFHLLMEHT TKVNPFGKPI TIEDVGDTAV FLCSDWARAI TGEVVHVDNG
260 270
YHIMGVFGRE EEIKKEVYGD
Length:270
Mass (Da):29,725
Last modified:May 30, 2000 - v2
Checksum:i2ACD6C85EE16DC68
GO

Sequence cautioni

The sequence AAC07465 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC07465.1 Different initiation.
PIRiG70434
RefSeqiNP_214070.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC07465; AAC07465; aq_1552
GeneIDi1193128
KEGGiaae:aq_1552
PATRICifig|224324.8.peg.1204

Similar proteinsi

Entry informationi

Entry nameiFABI_AQUAE
AccessioniPrimary (citable) accession number: O67505
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: May 23, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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