UniProtKB - O67463 (TRMD_AQUAE)
Protein
tRNA (guanine-N(1)-)-methyltransferase
Gene
trmD
Organism
Aquifex aeolicus (strain VF5)
Status
Functioni
Specifically methylates guanosine-37 in various tRNAs.By similarity
Catalytic activityi
- guanosine37 in tRNA + S-adenosyl-L-methionine = H+ + N1-methylguanosine37 in tRNA + S-adenosyl-L-homocysteineEC:2.1.1.228
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 115 | S-adenosyl-L-methionine; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- tRNA (guanine(37)-N(1))-methyltransferase activity Source: GO_Central
- tRNA (guanine-N1-)-methyltransferase activity Source: GO_Central
GO - Biological processi
- tRNA N1-guanine methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | tRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | AAEO224324:G1G15-1064-MONOMER |
BRENDAi | 2.1.1.228, 396 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (guanine-N(1)-)-methyltransferase (EC:2.1.1.228)Alternative name(s): M1G-methyltransferase tRNA [GM37] methyltransferase |
Gene namesi | Name:trmD Ordered Locus Names:aq_1489 |
Organismi | Aquifex aeolicus (strain VF5) |
Taxonomic identifieri | 224324 [NCBI] |
Taxonomic lineagei | Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060317 | 1 – 257 | tRNA (guanine-N(1)-)-methyltransferaseAdd BLAST | 257 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 224324.aq_1489 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O67463 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O67463 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 134 – 139 | S-adenosyl-L-methionine bindingBy similarity | 6 |
Sequence similaritiesi
Belongs to the RNA methyltransferase TrmD family.Curated
Phylogenomic databases
eggNOGi | COG0336, Bacteria |
HOGENOMi | CLU_047363_0_1_0 |
InParanoidi | O67463 |
OMAi | ILCGHYK |
OrthoDBi | 525632at2 |
Family and domain databases
Gene3Di | 1.10.1270.20, 1 hit 3.40.1280.10, 1 hit |
HAMAPi | MF_00605, TrmD, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR002649, tRNA_m1G_MeTrfase_bac IPR023148, tRNA_m1G_MeTrfase_C IPR029026, tRNA_m1G_MTases_N IPR016009, tRNA_MeTrfase_TRMD/TRM10 |
PANTHERi | PTHR46417, PTHR46417, 1 hit |
Pfami | View protein in Pfam PF01746, tRNA_m1G_MT, 1 hit |
PIRSFi | PIRSF000386, tRNA_mtase, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00088, trmD, 1 hit |
i Sequence
Sequence statusi: Complete.
O67463-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSNPLRFFV LTIFPHIISC YSEYGIVKQA IKKGKVEVYP IDLREFAPKG
60 70 80 90 100
QVDDVPYGGL PGMVLKPEPI YEAYDYVVEN YGKPFVLITE PWGEKLNQKL
110 120 130 140 150
VNELSKKERI MIICGRYEGV DERVKKIVDM EISLGDFILS GGEIVALAVI
160 170 180 190 200
DAVSRVLPGV LSEPQSIQED SFQNRWLGYP VYTRPREYRG MKVPEELLSG
210 220 230 240 250
HHKLIELWKL WHRIENTVKK RPDLIPKDLT ELEKDILNSI LSGKSFKEWL
KEHKHLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC07418.1 |
PIRi | E70429 |
RefSeqi | NP_214028.1, NC_000918.1 WP_010880966.1, NC_000918.1 |
Genome annotation databases
EnsemblBacteriai | AAC07418; AAC07418; aq_1489 |
KEGGi | aae:aq_1489 |
PATRICi | fig|224324.8.peg.1161 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC07418.1 |
PIRi | E70429 |
RefSeqi | NP_214028.1, NC_000918.1 WP_010880966.1, NC_000918.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OY5 | X-ray | 2.60 | A/B/C | 1-257 | [»] | |
SMRi | O67463 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224324.aq_1489 |
Genome annotation databases
EnsemblBacteriai | AAC07418; AAC07418; aq_1489 |
KEGGi | aae:aq_1489 |
PATRICi | fig|224324.8.peg.1161 |
Phylogenomic databases
eggNOGi | COG0336, Bacteria |
HOGENOMi | CLU_047363_0_1_0 |
InParanoidi | O67463 |
OMAi | ILCGHYK |
OrthoDBi | 525632at2 |
Enzyme and pathway databases
BioCyci | AAEO224324:G1G15-1064-MONOMER |
BRENDAi | 2.1.1.228, 396 |
Miscellaneous databases
EvolutionaryTracei | O67463 |
Family and domain databases
Gene3Di | 1.10.1270.20, 1 hit 3.40.1280.10, 1 hit |
HAMAPi | MF_00605, TrmD, 1 hit |
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR002649, tRNA_m1G_MeTrfase_bac IPR023148, tRNA_m1G_MeTrfase_C IPR029026, tRNA_m1G_MTases_N IPR016009, tRNA_MeTrfase_TRMD/TRM10 |
PANTHERi | PTHR46417, PTHR46417, 1 hit |
Pfami | View protein in Pfam PF01746, tRNA_m1G_MT, 1 hit |
PIRSFi | PIRSF000386, tRNA_mtase, 1 hit |
SUPFAMi | SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00088, trmD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRMD_AQUAE | |
Accessioni | O67463Primary (citable) accession number: O67463 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | August 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families