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Entry version 130 (02 Dec 2020)
Sequence version 1 (01 Aug 1998)
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Protein

DNA topoisomerase 1

Gene

topA

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation EC:5.6.2.1

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi7Magnesium 1; catalyticUniRule annotation1
Metal bindingi79Magnesium 1; catalyticUniRule annotation1
Metal bindingi79Magnesium 2UniRule annotation1
Metal bindingi81Magnesium 2UniRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei281O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
AAEO224324:G1G15-473-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.6.2.1UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:aq_657
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiAquifex aeolicus (strain VF5)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224324 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000798 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001451381 – 540DNA topoisomerase 1Add BLAST540

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
O66893

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei31Interaction with DNAUniRule annotation1
Sitei136Interaction with DNAUniRule annotation1
Sitei137Interaction with DNAUniRule annotation1
Sitei140Interaction with DNAUniRule annotation1
Sitei145Interaction with DNAUniRule annotation1
Sitei152Interaction with DNAUniRule annotation1
Sitei283Interaction with DNAUniRule annotation1
Sitei467Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224324.aq_657

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
O66893

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini1 – 110ToprimUniRule annotationAdd BLAST110

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni161 – 166Interaction with DNAUniRule annotation6

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0550, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_002929_4_3_0

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O66893

Identification of Orthologs from Complete Genome Data

More...
OMAi
PECKYTR

Database of Orthologous Groups

More...
OrthoDBi
223233at2

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00186, TOP1Ac, 1 hit
cd03363, TOPRIM_TopoIA_TopoI, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.290.10, 1 hit
1.10.460.10, 1 hit
2.70.20.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00952, Topoisom_1_prok, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000380, Topo_IA
IPR003601, Topo_IA_2
IPR023406, Topo_IA_AS
IPR013497, Topo_IA_cen
IPR013824, Topo_IA_cen_sub1
IPR013825, Topo_IA_cen_sub2
IPR013826, Topo_IA_cen_sub3
IPR023405, Topo_IA_core_domain
IPR003602, Topo_IA_DNA-bd_dom
IPR005733, TopoI_bac-type
IPR028612, Topoisom_1_IA
IPR006171, TOPRIM_domain
IPR034149, TOPRIM_TopoI

The PANTHER Classification System

More...
PANTHERi
PTHR42785, PTHR42785, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01131, Topoisom_bac, 1 hit
PF01751, Toprim, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00417, PRTPISMRASEI

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00437, TOP1Ac, 1 hit
SM00436, TOP1Bc, 1 hit
SM00493, TOPRIM, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56712, SSF56712, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01051, topA_bact, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00396, TOPOISOMERASE_I_PROK, 1 hit
PS50880, TOPRIM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O66893-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELFIVESPT KAKTIQKFLG KGFLVKATLG HVKDLPEKEL GVDLRTLKAK
60 70 80 90 100
YVYKRGKKKL VEQLKKLSRR SSIVYLGTDP DREGEAIAYF LKKDLEKVNK
110 120 130 140 150
NIKRAVFYEI TPEAIRESIR NAGDVNMNLV YAQFARRILD RLIGYLISPI
160 170 180 190 200
LWKEFKNYKL SAGRVQSPAL RLIVEREREI QNFKVKKYYY VKALLRKGSE
210 220 230 240 250
EFWAIYDYRY ENPSDAKIIA KKLEKGYFSV YKVEKKKEKV SPPKPFITSD
260 270 280 290 300
LQSEANAKFG FSSERTQKLA QELYEKGYIT YPRTDSYRMN EKKAKEFMNY
310 320 330 340 350
IEKKYGKEYV GRLRRFREKA TAQGAHECIR PTSLREEIPE REELRLLYDL
360 370 380 390 400
IFRRTIASLM KEMLLEREKV TVEAITPELK HPVYLVAKGL KIVFDGWSRV
410 420 430 440 450
YPSEITEEKL PELYEGDLLD LVKTTLEERK TQPPPRYTEG TLIKTLEKLG
460 470 480 490 500
IGRPSTYATI VKTLKERGYV EVKKKALVPT EIAFQVVEFL MERFPTLMDY
510 520 530 540
KFTAQMEEKL DLVEEGKLNW KSVVYEFMEK IFGKELEMVR
Length:540
Mass (Da):63,427
Last modified:August 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i89C0604DFF5B042E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC06848.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A70358

NCBI Reference Sequences

More...
RefSeqi
NP_213453.1, NC_000918.1
WP_010880391.1, NC_000918.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC06848; AAC06848; aq_657

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
aae:aq_657

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224324.8.peg.534

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC06848.1
PIRiA70358
RefSeqiNP_213453.1, NC_000918.1
WP_010880391.1, NC_000918.1

3D structure databases

SMRiO66893
ModBaseiSearch...

Protein-protein interaction databases

STRINGi224324.aq_657

Proteomic databases

PRIDEiO66893

Genome annotation databases

EnsemblBacteriaiAAC06848; AAC06848; aq_657
KEGGiaae:aq_657
PATRICifig|224324.8.peg.534

Phylogenomic databases

eggNOGiCOG0550, Bacteria
HOGENOMiCLU_002929_4_3_0
InParanoidiO66893
OMAiPECKYTR
OrthoDBi223233at2

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-473-MONOMER

Family and domain databases

CDDicd00186, TOP1Ac, 1 hit
cd03363, TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 1 hit
2.70.20.10, 1 hit
HAMAPiMF_00952, Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR000380, Topo_IA
IPR003601, Topo_IA_2
IPR023406, Topo_IA_AS
IPR013497, Topo_IA_cen
IPR013824, Topo_IA_cen_sub1
IPR013825, Topo_IA_cen_sub2
IPR013826, Topo_IA_cen_sub3
IPR023405, Topo_IA_core_domain
IPR003602, Topo_IA_DNA-bd_dom
IPR005733, TopoI_bac-type
IPR028612, Topoisom_1_IA
IPR006171, TOPRIM_domain
IPR034149, TOPRIM_TopoI
PANTHERiPTHR42785, PTHR42785, 1 hit
PfamiView protein in Pfam
PF01131, Topoisom_bac, 1 hit
PF01751, Toprim, 1 hit
PRINTSiPR00417, PRTPISMRASEI
SMARTiView protein in SMART
SM00437, TOP1Ac, 1 hit
SM00436, TOP1Bc, 1 hit
SM00493, TOPRIM, 1 hit
SUPFAMiSSF56712, SSF56712, 1 hit
TIGRFAMsiTIGR01051, topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396, TOPOISOMERASE_I_PROK, 1 hit
PS50880, TOPRIM, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTOP1_AQUAE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O66893
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: December 2, 2020
This is version 130 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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