UniProtKB - O66841 (NUOF_AQUAE)
Protein
NADH-quinone oxidoreductase subunit F
Gene
nuoF
Organism
Aquifex aeolicus (strain VF5)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- FMNCuratedNote: Binds 1 FMN.Curated
- [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 347 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 350 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 353 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 | |
Metal bindingi | 393 | Iron-sulfur (4Fe-4S)Sequence analysis | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 65 – 74 | NADBy similarity | 10 | |
Nucleotide bindingi | 176 – 223 | FMNBy similarityAdd BLAST | 48 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- FMN binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- NADH dehydrogenase (ubiquinone) activity Source: InterPro
- quinone binding Source: UniProtKB-KW
Keywordsi
Molecular function | Translocase |
Ligand | 4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | AAEO224324:G1G15-418-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit F (EC:7.1.1.-)Alternative name(s): NADH dehydrogenase I subunit F NDH-1 subunit F |
Gene namesi | Name:nuoF Ordered Locus Names:aq_573 |
Organismi | Aquifex aeolicus (strain VF5) |
Taxonomic identifieri | 224324 [NCBI] |
Taxonomic lineagei | Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118570 | 1 – 426 | NADH-quinone oxidoreductase subunit FAdd BLAST | 426 |
Keywords - PTMi
QuinoneStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O66841 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the complex I 51 kDa subunit family.Curated
Phylogenomic databases
eggNOGi | COG1894, Bacteria |
HOGENOMi | CLU_014881_0_1_0 |
InParanoidi | O66841 |
OMAi | CREGTDW |
OrthoDBi | 688908at2 |
Family and domain databases
Gene3Di | 1.20.1440.230, 1 hit 3.40.50.11540, 1 hit |
InterProi | View protein in InterPro IPR001949, NADH-UbQ_OxRdtase_51kDa_CS IPR011538, Nuo51_FMN-bd IPR037225, Nuo51_FMN-bd_sf IPR019575, Nuop51_4Fe4S-bd IPR037207, Nuop51_4Fe4S-bd_sf IPR019554, Soluble_ligand-bd |
Pfami | View protein in Pfam PF01512, Complex1_51K, 1 hit PF10589, NADH_4Fe-4S, 1 hit PF10531, SLBB, 1 hit |
SMARTi | View protein in SMART SM00928, NADH_4Fe-4S, 1 hit |
SUPFAMi | SSF140490, SSF140490, 1 hit SSF142019, SSF142019, 1 hit |
PROSITEi | View protein in PROSITE PS00644, COMPLEX1_51K_1, 1 hit PS00645, COMPLEX1_51K_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O66841-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRSYPAIPRI YAETTLNMLL KRAKKPRVHS IDEYLKDGGY QALEKALNMS
60 70 80 90 100
PEEIIDWVDK STLRGRGGAG FPTGKKWKFA VQNPGPRYFI CNADESEPGT
110 120 130 140 150
FKDRIIIERD PHLLIEGIII SSYAIGANEA YIYIRGEYPA GYYILRDAIE
160 170 180 190 200
EAKKKGFLGK NILGSGFDLE IYVARGAGAY ICGEETALIE SLEGKRGHPR
210 220 230 240 250
LKPPYPVQKG LWGKPTVVNN VETIANVPFI ISMGWEEYRY IGPSDYAGPK
260 270 280 290 300
LFPVSGKVKK PGVYELPMNT TLREVIFKYA GGTLGNKKVK AVFSGALDCF
310 320 330 340 350
SSEELDIPMD YSPLGFGGTG TVIVLTEEDD IVEAALKIAE FYEHETCGQC
360 370 380 390 400
TPCRVGCYEQ ANLLEKIYKG EATEQDWEGF DFVNRNIQPT SICGLGAVAG
410 420
RLIRQTLEKF PEEWEKYRKK SASLPL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC06800.1 |
PIRi | E70351 |
RefSeqi | NP_213401.1, NC_000918.1 WP_010880339.1, NC_000918.1 |
Genome annotation databases
EnsemblBacteriai | AAC06800; AAC06800; aq_573 |
KEGGi | aae:aq_573 |
PATRICi | fig|224324.8.peg.468 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC06800.1 |
PIRi | E70351 |
RefSeqi | NP_213401.1, NC_000918.1 WP_010880339.1, NC_000918.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6HL2 | X-ray | 1.95 | B/D | 1-426 | [»] | |
6HL3 | X-ray | 2.04 | B/D | 1-426 | [»] | |
6HL4 | X-ray | 2.06 | B/D | 1-426 | [»] | |
6HLA | X-ray | 1.90 | B/D | 1-426 | [»] | |
6HLI | X-ray | 2.38 | B/D | 1-426 | [»] | |
6HLJ | X-ray | 2.10 | B/D | 1-426 | [»] | |
6HLM | X-ray | 1.80 | B/D | 1-426 | [»] | |
6Q9C | X-ray | 1.78 | B/D | 2-419 | [»] | |
6Q9G | X-ray | 2.10 | B/D | 1-426 | [»] | |
6Q9J | X-ray | 1.83 | B/D | 1-426 | [»] | |
6Q9K | X-ray | 1.99 | B/D | 2-419 | [»] | |
6R7P | X-ray | 3.22 | B/D | 1-426 | [»] | |
6SAQ | X-ray | 2.02 | B/D | 1-426 | [»] | |
SMRi | O66841 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224324.aq_573 |
Genome annotation databases
EnsemblBacteriai | AAC06800; AAC06800; aq_573 |
KEGGi | aae:aq_573 |
PATRICi | fig|224324.8.peg.468 |
Phylogenomic databases
eggNOGi | COG1894, Bacteria |
HOGENOMi | CLU_014881_0_1_0 |
InParanoidi | O66841 |
OMAi | CREGTDW |
OrthoDBi | 688908at2 |
Enzyme and pathway databases
BioCyci | AAEO224324:G1G15-418-MONOMER |
Family and domain databases
Gene3Di | 1.20.1440.230, 1 hit 3.40.50.11540, 1 hit |
InterProi | View protein in InterPro IPR001949, NADH-UbQ_OxRdtase_51kDa_CS IPR011538, Nuo51_FMN-bd IPR037225, Nuo51_FMN-bd_sf IPR019575, Nuop51_4Fe4S-bd IPR037207, Nuop51_4Fe4S-bd_sf IPR019554, Soluble_ligand-bd |
Pfami | View protein in Pfam PF01512, Complex1_51K, 1 hit PF10589, NADH_4Fe-4S, 1 hit PF10531, SLBB, 1 hit |
SMARTi | View protein in SMART SM00928, NADH_4Fe-4S, 1 hit |
SUPFAMi | SSF140490, SSF140490, 1 hit SSF142019, SSF142019, 1 hit |
PROSITEi | View protein in PROSITE PS00644, COMPLEX1_51K_1, 1 hit PS00645, COMPLEX1_51K_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOF_AQUAE | |
Accessioni | O66841Primary (citable) accession number: O66841 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | August 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families